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投影片 1
投影片 1

... of Axin. This region is required for GSK3b binding. Expression of GSKIP inhibits the capacity of GSK-3 to phosphorylate certain substrates such as b-catenin. ...
The role of xylulokinase in Saccharomyces cerevisiae xylulose
The role of xylulokinase in Saccharomyces cerevisiae xylulose

... 3.2. Growth on D-xylulose To study whether the xylulokinase expression level is limiting the D-xylulose fermentation and whether an over-expression is detrimental for growth on D-xylulose, we over-expressed the XKS1 gene in the S. cerevisiae strain W303-1B. The doubling time of the W303-1B parent st ...
Np73 is capable of inducing apoptosis by co
Np73 is capable of inducing apoptosis by co

... N isoforms, lacking the TA domain but harbouring a DNAbinding domain and an oligomerization domain (OD), act as dominant negatives of the TA isoforms, binding to them and competing for the binding sites, but impairing transactivation. Thus, by interfering the proapoptotic and cell cycle regulatory ...
POSTER Vp-1
POSTER Vp-1

... Aspergillus niger is well known for its ability to secrete a wide variety of plant Carbohydrate Modifying Enzymes (CMEs). The secreted CMEs are able to degrade the plant cell wall materials as well as its storage carbohydrates, like starch and inulin. Based on its recently finished genomic sequence ...
PROTEIN EXTRACTION AND PURIFICATION
PROTEIN EXTRACTION AND PURIFICATION

... phosphate buffer at pH 6.6 rises to 6.9. Another 10-fold dilution increases the pH to 7.1. *Tris is particularly notorious for its large dissociation constant shift with concentration and temperature. Tris decreases approximately 0.1 pH unit per 10-fold dilution. So does temperature. If you happen t ...
Expanded protein information at SGD: new pages and proteome browser.
Expanded protein information at SGD: new pages and proteome browser.

... The main Protein Information page has been redesigned to provide basic protein information clearly and concisely with a familiar and readily navigable layout similar to that of the SGD Locus Summary page. The top section of the page, devoted to nomenclature, provides standard and systematic protein ...
Identification and characterization of heavy metal induced
Identification and characterization of heavy metal induced

... A second heavy metal marker gene analysed is homologous to a ClpD protein. Most knowledge about the Clp protease is derived from the studies of the model enzyme of Escherichia coli. The Clp holoenzyme consists of two adjacent heptameric rings of the proteolytic subunit known as ClpP, which are flank ...
Plant Enzyme Structure. Explaining Substrate
Plant Enzyme Structure. Explaining Substrate

... or more amino acid residues are likely to have similar 3D conformations. If the 3D structure of one such protein is known, the structure of the other can be deduced by homology modeling (9). Structures obtained by modeling will be less reliable than those determined experimentally by x-ray crystallo ...
Molecular and General Genetics
Molecular and General Genetics

... As shown in Table 3, OmpF protein is also required specifically for the activity of colicin N. To determine whether OmpF protein is directly involved in the colicin N receptor site, the binding of radioactively labeled colicin N to isogenic E. coli K12 strains was studied. Strains producing no porin ...
File - Pi Beta Philes!
File - Pi Beta Philes!

... since about 100 genes, and therefore proteins, are involved in weight, successful drug therapy is unlikely with the completion of the Human Genome Project, we have now identified the genes responsible for weight gain, so successful drug therapy is inevitable drugs have so many side effects that they ...
Phylogenetic and genetic analysis of envelope gene of the
Phylogenetic and genetic analysis of envelope gene of the

... A fresh wave of Dengue infection, particularly Dengue serotype 1and 3, have been observed all across India in recent times and has led to several fatalities. Since the surface situated envelope protein of the dengue virion is responsible for virus entry into the host cell, we have laid special empha ...
- Wiley Online Library
- Wiley Online Library

... NHase encoded by gene AB-strepP(C-sixH) was purified (Fig. 1c) and used to compare with the wild-type NHase. 70% activity of the wild-type NHase still remained in this mutant (Table 1), indicating that these His residues only partially participate in P14K function. In addition, residue Arg96, which ...
The N-end rule pathway as a nitric oxide sensor controlling the
The N-end rule pathway as a nitric oxide sensor controlling the

... Mammalian R-transferases are strong sequelogs1 of yeast (fungal) ATE1 R-transferases. However, while the inactivation of mouse ATE1 results in embryonic lethality2, a deletion of S. cerevisiae ATE1 renders cells unable to degrade reporters with N-terminal Asp or Glu but has not been found to cause a ...
Molecular Weight Determination by SDS-PAGE - Bio-Rad
Molecular Weight Determination by SDS-PAGE - Bio-Rad

... structure on migration. In addition, a strong ionic detergent such as SDS is a required component of the sample buffer. SDS provides two functions: It denatures secondary, tertiary, and quaternary structures by binding to hydrophobic protein regions, and its binding confers a net negative charge on ...
Plant and Soil 287:
Plant and Soil 287:

... Morganella morganii was transferred to Burkholderia cepacia IS-16, a strain used as a biofertilizer, using the broad-host range vector pRK293 (Fraga et al, 2001). An increase in extracellular phosphatase activity of the recombinant strain was achieved. Insertion of the transferred genes into the bac ...
sc-101769 (Page 1) - Santa Cruz Biotechnology, Inc.
sc-101769 (Page 1) - Santa Cruz Biotechnology, Inc.

... The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p5 ...
Unit 10 web
Unit 10 web

... • 15 different PNAH’s have been isolated from the outer layer of charcoal broiled steak • 8 micrograms of 1,2-benzopyrene per kilo of steak • Arise from decomposition of fat that drips on to the glowing charcoal and the subsequent vaporization of the hydrocarbons and deposition on the surface of the ...
03_Physical-chemical properties of proteins
03_Physical-chemical properties of proteins

... Glacial Acetic acid and Reddish violet ...
Document
Document

... Now not all genes are going to be silenced for the life of the cell/organism… Ex. The genes coding for enzymes that make glycogen in the liver… If the blood glucose concentration is low, the liver will be releasing glucose, not building glycogen from it. Therefore, the genes should be off. Likewise ...
Thiol regulation of pro-inflammatory cytokines and innate immunity
Thiol regulation of pro-inflammatory cytokines and innate immunity

... http://ngrams.googlelabs.com). Both inflammatory cytokines, particularly IL (interleukin)-1 and TNF (tumour necrosis factor), and oxidative stress have been implicated in so many diseases that it would be difficult to find one where neither has been involved. Both are interpreted with an ‘axis-of-ev ...
Cdc45: the missing RecJ ortholog in eukaryotes?
Cdc45: the missing RecJ ortholog in eukaryotes?

... Fig. 1. Sequence analysis of the Cdc45 and RecJ protein families. (A) Domain architecture of human Cdc45 and Escherichia coli RecJ proteins. For the E.coli RecJ protein, domains were assigned according to the RecJ core structure (Yamagata et al., 2002) and the Pfam domain database (Finn et al., 2008 ...
Archives of microbiology
Archives of microbiology

... affects the nitrogenase activity and the expression of nifH and nifA genes, (Rosconi et al. 2006). Iron is one of the most abundant elements in the Earth’s crust, but it is biologically unavailable. In an oxidative environment at a neutral pH, ferric iron (Fe3+) predominates and it is the stable for ...
Intrinsically unstructured proteins
Intrinsically unstructured proteins

... TATA-box-associated factors (TAFs) in the assembly of the transcription preinitiation complex and is also subtly regulated by phosphorylation. In general, the IUPs in all five functional classes are involved in the regulation of key cellular processes, such as transcription, translation, signal tran ...
INSILICO MODELING OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS PROTEIN STREPTOCOCCUS PNEUMONIAE LIGAND IDENTIFICATION
INSILICO MODELING OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS PROTEIN STREPTOCOCCUS PNEUMONIAE LIGAND IDENTIFICATION

... genes are also involved in modulation of capsule synthesis in Streptococcus pneumonia[2]. These genes codes capsular polysaccharide biosynthesis proteins. Proteins coded by these genes are tyrosine phosphatase, capsular polysaccharide biosynthesis protein and tyrosine kinase respectively [3]. Strept ...
Protein
Protein

... DEVELOPMENT OF E-COURSES FOR B.F.Sc COURSES TOPIC: Structure of protein COURSE ID: ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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