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Caffeine as a cause of coral bleaching: Effects of caffeine on
Caffeine as a cause of coral bleaching: Effects of caffeine on

... marine life. Studies have shown that low concentrations of caffeine can induce bleaching in coral; i.e. caffeine causes coral to release their algal symbionts (zooxanthellae). Corals may recover from this, but bleaching events often lead to death. We hypothesized that caffeine causes the zooxanthell ...
antisense orfs, codon bias and the evo lu tion of the ge netic code
antisense orfs, codon bias and the evo lu tion of the ge netic code

... bias with an AT-drift. This AT-drift pattern occurs in HSP-70 proteins of AT-rich species and illustrates the role of the wobble base in the evolution of genes for specific proteins from a GC-rich to an AT-rich species. Together with Carter (Cell 10, 705-808, 2002) we have suggested that (a) the exi ...
TRANSLASI - alanindra
TRANSLASI - alanindra

... initiation sites. There can be several different initiation sites on a messenger RNA: a prokaryotic mRNA can code for several different proteins. Translation begins at an AUG codon, or sometimes a GUG. The modified amino acid Nformyl methionine is always the first amino acid of the new polypeptide. ...
Chapter 19 Regulation of Gene Expression in Prokaryotes and Their
Chapter 19 Regulation of Gene Expression in Prokaryotes and Their

... • In positive control mechanisms, the product of a regulator gene, an activator, is required to turn on the expression of the structural gene(s). • In negative control mechanisms, the product of a regulator gene, a co-repressor, is required to turn off the expression of the structural gene(s). • Act ...
Macromolecules: Proteins Chapter 3 pages 44
Macromolecules: Proteins Chapter 3 pages 44

... protein, actin, for example. Tau is a protein found with cell structure microtubules.) • Healing: ßAPP triggers inflammatory response and acts as anti-coagulant to prevent blood clots. • May increase the expression of specific genes inside the cells. • Why would releasing ß-APP cause harm? ...
Expression profiles of Ciona tailbud embryos - Development
Expression profiles of Ciona tailbud embryos - Development

... muscle. Many genes were also specifically expressed in multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were ...
Procedure and Troubleshooting
Procedure and Troubleshooting

... Continue with step 2! C. Third option: Seamless cloning using restriction free methods can be used to insert DNA sequences at any place of MultiLabel vectors. Add a 15–20 bases 5′-tail to the primers containing DNA sequences that are homologous to the sequences flanking the site of insertion into th ...
Protein and Amino Acids
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... potentially available to contribute to the amino acid pool, whereas those that pass to the ________ are not.  Does quality of protein generally dictate where ...
SAM Teachers Guide - RI
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...  What are some specific jobs of proteins that require them to have a distinct 3D structure? (Possible answers: enzymes, roles in signal transduction, DNA synthesis, etc.)  What types of situations may impact how a protein would function? Generate ideas about temperature, whether it is surrounded b ...
Spnr, a Murine RNA-binding Protein That Is Localized to
Spnr, a Murine RNA-binding Protein That Is Localized to

... Since 3' UTR sequences are involved in many aspects of mRNA metabolism, including translational control, we were interested in finding proteins that could bind to sequences within the 3' UTR of the spermatid-specific protamine 1 (Prmd) mRNA. The mouse Prmd gene encodes a male germ cell-specific mRNA ...
A mutation which disrupts the hydrophobic core of the signal peptide
A mutation which disrupts the hydrophobic core of the signal peptide

... concentrations of 2 8 6 + 4 4 and 286+ 16 respectively. Serum bilirubin of the patients currently is 1 9 5 + 2 ~tM, patient A, and 197 +3 p.M, patient B. Both patients are treated with phenobarbital, and patient A also receives phototherapy. Treatment with phenobarbital reduced serum bilirubin by 49 ...
plantcell.org - Schnable Lab
plantcell.org - Schnable Lab

... Furthermore, this method is limited to surface cells, because it is difficult to identify specific cell types in the center of intact organs. To identify nonsurface cells, Brandt et al. (1999) developed transgenic plants that carry a nonsurface cell (e.g., companion cell)–specific promoter fused to ...
Crenarchaeal CdvA Forms Double-Helical Filaments Containing
Crenarchaeal CdvA Forms Double-Helical Filaments Containing

... Eukarya and Bacteria [1]. Although Archaea resemble Bacteria in terms of size, cell structure and genome organization, they are often much more similar to Eukarya at the molecular level. This is true both for informational mechanisms, such as replication, transcription and translation, as well as fo ...
Expression and V (D) J recombination activity of mutated RAG
Expression and V (D) J recombination activity of mutated RAG

... unmodified RAG-1 expression plasmid, pJH548, gave 0.4% recombination. Truncation of the C-terminal 31 residues to amino acid 1009 (plasmid pMS106) had little measurable effect. However, further truncation to residue 994 (plasmid pMS108) eliminated recombination activity. This result and that of anot ...
Nerve activates contraction
Nerve activates contraction

... • A functional proteins consists of one or more polypeptides that have been precisely twisted, folded, and coiled into a unique shape. • It is the order of amino acids that determines what the three-dimensional conformation will be. ...
Differential gene expression in ES/PNET with type 1 vs type 2 EWS
Differential gene expression in ES/PNET with type 1 vs type 2 EWS

... Performance of microarray-based predictor 136/137 sarcomas were accurately predicted Model Prediction ...
mschi
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... engineering goal for the past several years, primarily due to the purported health-promoting effects of these compounds. Although significant strides have been made recently in improving strain titers and yields, current fermentation strategies suffer from two major drawbacks—(1) the requirement for ...
novel 4E-interacting protein in Leishmania is involved in stage
novel 4E-interacting protein in Leishmania is involved in stage

... nucleotides (m7Gpppm36,6,2 Apm2 Apm2 Cpm23,2 U), as compared to the simple m7GTP cap used in higher eukaryotes, and is denoted cap-4 (14). Since protein coding genes in Leishmania are not regulated by transcriptional activation, translation regulation is believed to play a key role in driving the di ...
Case study - Castle High School
Case study - Castle High School

... CQ#3: Proteins such as the ß amyloid and the mutant ß amyloid that results in earlyonset Alzheimer’s differ from one another because: A. Peptide bonds linking the amino acids differ from one protein to another. B. The two proteins have a different combination of amino acids along the chain of the p ...
Protein design as an inverse problem
Protein design as an inverse problem

... • Proteins are used as treatments for many diseases. – Protein engineering has helped improve proteins that are given to cancer patients on radiation or chemo-therapy. – Work in the Green lab is on-going to design proteins for use as anti-HIV prophylatics. ...
Lecture 33
Lecture 33

... …in sickness and in health protein synthesis is tightly regulated by environmental stimuli as well as intrinsic processes (e.g., hormonal, developmental). dysregulation can cause disease. many antibiotics act at the level of protein synthesis. ...
Structure and function of proteins controlling strain
Structure and function of proteins controlling strain

... Pseudomonas syringae AvrPto proteins, domain swap experiments between Pto and another closely related gene, Fen, at the complex Pto locus and yeast two-hybrid analysis of the chimeric genes have identified a small region of Pto involved in the interaction with AvrPto and thus also involved in the de ...
Structure of the Gene Coding for the a Polypeptide Chain of
Structure of the Gene Coding for the a Polypeptide Chain of

... The human gene coding for the 70-kD polypeptide of the complement regulatory component C4b-binding protein (C4BPa) spans over 40 kb of DNA and is composed of twelve exons. Upon transcription in liver, or in Hep-G2 cells, this gene produces a single transcript of 2,262 nucleotides, excepting the poly ...
List of protein families currently covered by SVMProt
List of protein families currently covered by SVMProt

... Appendix S2 Method for computing the feature vector of a protein sequence A protein sequence is represented by specific feature vector assembled from encoded representations of tabulated residue properties including amino acid composition, hydrophobicity, normalized Van der Waals volume, polarity, p ...
Moving Proteins into Membranes and Organelles Moving Proteins
Moving Proteins into Membranes and Organelles Moving Proteins

... ensuring that misfolded proteins do not leave ER. In addition to co-translational modifications, the correct folding/assembly may require the presence of a group of proteins called chaperones. Some chaperones (e.g. BiP) have high affinity toward unfolded proteins in general, yet others (e.g. calreti ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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