• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Supplementary Methods (a) Chemically
Supplementary Methods (a) Chemically

... proteins across the complete dataset were grouped into clusters by generating a similarity matrix ...
Gene Expression Profiling During Asexual Development of the Late
Gene Expression Profiling During Asexual Development of the Late

... be purified from hyphae, and each developmental stage described above can be induced without a plant host, which is not the case for many oomycetes (Hardham and Hyde 1997). Also, progress has begun to be made toward identifying genes and proteins involved in the different life stages. For example, e ...
Proteins
Proteins

... (fully assembled) ...
TNT SP6 High-Yield Wheat Germ Protein Expression
TNT SP6 High-Yield Wheat Germ Protein Expression

... The TnT® SP6 High-Yield Wheat Germ Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system designed to express up to 100µg/ml of protein. This cell-free expression system is prepared from an optimized wheat germ extract and contains all the components ...
Microarray on Germinating Yeast Spores (WP2)
Microarray on Germinating Yeast Spores (WP2)

... • The aim of my project is to uncover how eukaryotic cells maintain dormant stages and how they are again reactivated • We are using the ordinary baker’s yeast Saccharomyces cerevisiae as model organism • S. cerevisiae produces a dormant stage in the form of the yeast spore ...
Slide 1
Slide 1

... • The most important type of antibody bound by protein G and not normally by protein A is mouse IgG1 which is the most common subclass of monoclonal antibodies. However, the addition of high salt (2-3 M NaCl) with high pH (8-9) to the binding / wash buffer will cause these antibodies to bind. ...
Biol115_2014_Lecture 8_Protein Structure
Biol115_2014_Lecture 8_Protein Structure

... Domains are the functional toolkits of proteins • There are relatively few unique domains…maybe <500. • When mixed in different combinations, domains provide all of the biological functions for life. • e.g. fibronectin III domain – found in 3427 known proteins Insulin-like growth factor receptor (m ...
Lesson on Proteins
Lesson on Proteins

... What do you think might happen to an organism that stopped making copies of its DNA? The DNA would become damaged and the organism would have a difficult time producing proteins, it would eventually die. Why are proteins considered polymers? They are made from repeating sub-units that are linked tog ...
Protein kinase Protein kinases are enzymes that add a phosphate
Protein kinase Protein kinases are enzymes that add a phosphate

... other family of human enzymes except proteases. Workers have identified 478 typical and 40 atypical protein kinase genes in humans (total 518) that correspond to about 2% of all human genes (Table). Protein kinases have a similar overall three-dimensional structure and chemical mechanism. Protein-se ...
National  Library  of Medicine BuiMing  38A
National Library of Medicine BuiMing 38A

... matches between pairs of protein sequences. BLAST uses amino acid mutation scores for approximate stringmatching. It directly approximates the results that would be obtained by a dynamic programming algorithm, but is more than an order of magnitude faster. Given a pair of protein sequences, BLAST id ...
A conserved enhancer of the human and murine Hoxa
A conserved enhancer of the human and murine Hoxa

... Hox genes causes homeotic transformations to more anterior or posterior structures depending on the experimental system. Therefore identification of regulatory elements controlling the activity of homeotic genes, especially regulators defining the onset of expression in time and space, is of crucial ...
Stockholm University
Stockholm University

... effective for overexpression of membrane proteins [53-56, 61, 62]), there are also several examples where they have failed [63, 64]. Significantly, analyses of large data sets have failed to find a correlation between codon usage and overexpression levels of membrane proteins in E. coli and S. cerev ...
0 1R L Press Limited, Oxford, England.
0 1R L Press Limited, Oxford, England.

... gene, sak, was stably expressed in E. coli and the active product with a molecular weight and an antigenicity indistinguishable from those of authentic staphylokinase was efficiently secreted into the periplasmic space. Thus it can be a useful tool to investigate the mechanism of protein secretion i ...
protein folding - Federation of American Societies for Experimental
protein folding - Federation of American Societies for Experimental

... the degenerating nerve cells of Alzheimer’s disease eventually provided a key to understanding the disorder. It was development of the biotechnology industry that unexpectedly spurred interest in insoluble protein gunk. This industry can produce proteins (often otherwise difficult-to-obtain human pr ...
Protein-Protein Interactions
Protein-Protein Interactions

... the cytoplasm or even in membranes (such as Notch). They can be activated by translocation to the nucleus where they interact with other transcription factors in order to activate gene transcription. Transport is also important for proteins that are exported from cells such as peptide hormones. Stab ...
Computational Pharmacology - Carnegie Mellon School of
Computational Pharmacology - Carnegie Mellon School of

... have we understood how aspirin works, and how it might be improved http://www.rcsb.org/pdb/molecules/pdb17_1.html ...
Expanding the Genetic Code of Escherichia coli
Expanding the Genetic Code of Escherichia coli

... A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli. When introduced into E. coli, this pair leads to the in vivo incorporation of the synthetic amino acid O-methyl-L-tyrosine into protein in re ...
2 -1 -2 -1 1 2 K
2 -1 -2 -1 1 2 K

... units – domain family members are structurally and functionally conserved and recombined in complex ways during evolution  Domains can be seen as the units of evolution  Novelty in protein function often arises as a result of gain or loss of domains, or by re-shuffling existing domains along seque ...
Complete nucleotide sequence of RNA 4 of rice stripe virus isolate T
Complete nucleotide sequence of RNA 4 of rice stripe virus isolate T

... al., 1991). A large amount of a non-structural protein, the stripe disease-specific protein (S protein), is produced in RSV-infected rice plants (Kiso & Yamamoto, 1973; Toriyama, 1986); this protein has been shown to be encoded by the fourth longest segment, R N A 4 (Hayano et al., 1990). Toriyama ( ...
A1114-CFS-SD1 - Food Standards Australia New Zealand
A1114-CFS-SD1 - Food Standards Australia New Zealand

... the latter of which are used, for example, in breakfast cereals, baking products, extruded confectionery and food coatings. In 2011, Australia and New Zealand imported, respectively, 856 and 5,800 tonnes of corn grain, 10,600 and 306 tonnes of frozen sweet corn and 8,427 and 900 tonnes of otherwise- ...
91.510_ch9_2
91.510_ch9_2

... For >50% identity, RMSD is often only 1 Å. ...
jan15
jan15

... process… - If the mutations complement—they must be in ...
Identification of Plant Virus Movement-Host Protein
Identification of Plant Virus Movement-Host Protein

... structural protein, termed NSm, is translated from this cistron and that this protein represents the MP of the Tospoviruses (Kormelink et al., 1994). It is still a m atter of discussion whether the appearance of this specified additional cistron opened the door to attack organisms of the plant kingd ...
Comparative Analysis of Protein Content in Selected Meat Samples
Comparative Analysis of Protein Content in Selected Meat Samples

... sequence of amino acid residues in a protein is defined by the sequence of a gene, which is encoded in the genetic code. In general, the genetic code can include selenocysteine and in certain archea-pyrrolysine. Shortly after one or even during synthesis, the residues in a protein are often chemical ...
Identification of Plant Virus Movement
Identification of Plant Virus Movement

... structural protein, termed NSm, is translated from this cistron and that this protein represents the MP of the Tospoviruses (Kormelink et al., 1994). It is still a m atter of discussion whether the appearance of this specified additional cistron opened the door to attack organisms of the plant kingd ...
< 1 ... 106 107 108 109 110 111 112 113 114 ... 221 >

Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report