Application Note: Using the NanoDrop One to Quantify Protein and
... biomolecules (e.g., protein and nucleic acid preparations) as a routine part of their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification methods to choose from including gravimetric approaches, colorimet ...
... biomolecules (e.g., protein and nucleic acid preparations) as a routine part of their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification methods to choose from including gravimetric approaches, colorimet ...
Diapositive 1
... A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insertion of the protein in the membrane of an organelle (endoplasmic reticulum, mitochondria...) or translocation of the protein through one or several organelle membranes. When ...
... A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insertion of the protein in the membrane of an organelle (endoplasmic reticulum, mitochondria...) or translocation of the protein through one or several organelle membranes. When ...
Narcissus pseudonarcissus L. `Dutch Master`
... Results from an initial reverse Northern analysis that compared the abundance of the isolated sequences in the tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Ta ...
... Results from an initial reverse Northern analysis that compared the abundance of the isolated sequences in the tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Ta ...
Molecular cloning, characterization, and homologous
... morrisoni according to the methods of Cutting and van der Horn (10). PCR reaction mixtures contained 34 μL of dH2O, 5 μL of 10× buffer for Taq polymerase (Fermentas), 5 μL of 25 mM MgCl2, 50 pmol of each primer, 1 μL of 10 mM dNTP mix (Fermentas), 0.5 μg of chromosomal DNA and 3 units (1 μL) of Taq ...
... morrisoni according to the methods of Cutting and van der Horn (10). PCR reaction mixtures contained 34 μL of dH2O, 5 μL of 10× buffer for Taq polymerase (Fermentas), 5 μL of 25 mM MgCl2, 50 pmol of each primer, 1 μL of 10 mM dNTP mix (Fermentas), 0.5 μg of chromosomal DNA and 3 units (1 μL) of Taq ...
basic principles of isoelectric focusing in biomedical engineering
... electrical charge or the negative and positive charges are equal. Surfaces naturally charge to form a double layer. In the common case when the surface chargedetermining ions are H+/OH-, the net surface charge is affected by the pH of the liquid in which the solid is submerged. Again, the pI is the ...
... electrical charge or the negative and positive charges are equal. Surfaces naturally charge to form a double layer. In the common case when the surface chargedetermining ions are H+/OH-, the net surface charge is affected by the pH of the liquid in which the solid is submerged. Again, the pI is the ...
Nucleotide sequence of a cytomegalovirus single
... was estimated to be 3.9 kb in length (Anders & Gibson, 1988), consistent with the utilization of these flanking signals. Although the cap site has not been determined, upstream probes do not detect the 3.9 kb transcript, also consistent with the use of the T A T A box at - 43 to - 38 (Anders & Gibso ...
... was estimated to be 3.9 kb in length (Anders & Gibson, 1988), consistent with the utilization of these flanking signals. Although the cap site has not been determined, upstream probes do not detect the 3.9 kb transcript, also consistent with the use of the T A T A box at - 43 to - 38 (Anders & Gibso ...
Mean-field minimization methods for biological macromolecules
... method [6°°] is a direct application of the M F T described above. It is based on the rotamer library of Tuffery et a/. [28] and iteratively refines a conformational matrix (CM) of the side chains of a protein using Equations 9 and 10 ( C M is identical to the array v defined above). During the refi ...
... method [6°°] is a direct application of the M F T described above. It is based on the rotamer library of Tuffery et a/. [28] and iteratively refines a conformational matrix (CM) of the side chains of a protein using Equations 9 and 10 ( C M is identical to the array v defined above). During the refi ...
Conformational Analysis Protein Folding Protein Structure
... At the start, the algorithm creates random population of individuals (individual = conformer). Each individual is encoded by genes (gen = value of one dihedral angle). Fitness of individuals is evaluated (i.e. energy is calculated) and individuals with high fitness (low energy) are reproduced to ...
... At the start, the algorithm creates random population of individuals (individual = conformer). Each individual is encoded by genes (gen = value of one dihedral angle). Fitness of individuals is evaluated (i.e. energy is calculated) and individuals with high fitness (low energy) are reproduced to ...
Arabidopsis Contains Ancient Classes of Differentially Expressed
... Chua, 1999) examining both transcripts and promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclas ...
... Chua, 1999) examining both transcripts and promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclas ...
... constructs comprised of a promoter (to turn the recombinant gene ON or OFF), a signal peptide (to direct the translated protein to the chloroplast), a codon-optimized Ca1 gene (to match the high guanine/cytosine content of the algal genome), and an algal terminator (to ensure proper conclusion of mR ...
Food Chemistry
... (1976) method, adapted to micro-assays, uses bovine serum albumin as a standard. For evaluation, diluted samples and the Bradford reactive were placed in a 96-well micro-plate in triplicate. Absorbance was measured at 595 nm in a VersaMaxTM micro-plate reader (Molecular Devises, Sunnyvale, CA, USA). ...
... (1976) method, adapted to micro-assays, uses bovine serum albumin as a standard. For evaluation, diluted samples and the Bradford reactive were placed in a 96-well micro-plate in triplicate. Absorbance was measured at 595 nm in a VersaMaxTM micro-plate reader (Molecular Devises, Sunnyvale, CA, USA). ...
Prokaryotic Annotation at TIGR
... proteins from other species which have been experimentally characterized in that other species • only such “characterized matches” can be used as evidence for functional annotation • to help in our annotation process we have created a database storing accessions of proteins known to be experimentall ...
... proteins from other species which have been experimentally characterized in that other species • only such “characterized matches” can be used as evidence for functional annotation • to help in our annotation process we have created a database storing accessions of proteins known to be experimentall ...
Amino Acids and Proteins
... protein. The structural formulas of di- and tripeptides are written. The secondary forms of protein structure include the alpha helix, pleated sheet and collagen. The interaction of side groups to form the cross-links of tertiary structure is discussed. The breakdown in the secondary and tertiary st ...
... protein. The structural formulas of di- and tripeptides are written. The secondary forms of protein structure include the alpha helix, pleated sheet and collagen. The interaction of side groups to form the cross-links of tertiary structure is discussed. The breakdown in the secondary and tertiary st ...
PROTEINS Proteins are unbranched polymers of amino acids linked
... C. DERIVED PROTEINS: This class of proteins includes those protein products formed from the simple and conjugated proteins. It is not a well-defined class of proteins. These are produced by various physical and chemical factors and are divided in two major groups. (a) Primary derived proteins: Denat ...
... C. DERIVED PROTEINS: This class of proteins includes those protein products formed from the simple and conjugated proteins. It is not a well-defined class of proteins. These are produced by various physical and chemical factors and are divided in two major groups. (a) Primary derived proteins: Denat ...
... GM34496, Cancer Center (CORE) Support Grant CA21765from the determine if all of the biochemical reactions and physiological National Cancer Institute, and the American Lebanese Syrian Associated Charities. Thecosts of publication of this article were defrayed in roles attributed to 2-acyl-GPE acyltr ...
From DNA to Protein Structure and Function - Science Take-Out
... To help you understand how proteins are produced and how they function, you will model the processes of protein synthesis and protein folding. ...
... To help you understand how proteins are produced and how they function, you will model the processes of protein synthesis and protein folding. ...
A1981LF07700001
... that I received my postdoctoral training. "In the 1960s the emphasis was on the 'mechanism' of phytochrome action, i.e., the biophysical and molecular steps leading from the formation of active phytochrome to the final displays (photoresponses). As a convenient guiding principle in studies about the ...
... that I received my postdoctoral training. "In the 1960s the emphasis was on the 'mechanism' of phytochrome action, i.e., the biophysical and molecular steps leading from the formation of active phytochrome to the final displays (photoresponses). As a convenient guiding principle in studies about the ...
The anti-adipogenic effect of vitexin is associated with regulation of
... the conversion process and it is thus used as a classical marker for adipose cell differentiation (Pairault and Green, 1979). (3) Oxidative phosphorylation (OXPHOS). Before the introduction of free oxygen into the atmosphere, life on earth depended on glycolysis for energy production. With the rise ...
... the conversion process and it is thus used as a classical marker for adipose cell differentiation (Pairault and Green, 1979). (3) Oxidative phosphorylation (OXPHOS). Before the introduction of free oxygen into the atmosphere, life on earth depended on glycolysis for energy production. With the rise ...
Quality Components of Feeds
... fibre digestion and cause diarrhoea. Silages (additional quality indicators) 8. pH This is a measure of acidity. It is normally measured for silage and baleage. Well preserved and wetter silages normally have a lower pH, meaning more acid has been produced during the fermentation process. Drier sila ...
... fibre digestion and cause diarrhoea. Silages (additional quality indicators) 8. pH This is a measure of acidity. It is normally measured for silage and baleage. Well preserved and wetter silages normally have a lower pH, meaning more acid has been produced during the fermentation process. Drier sila ...
Structural Bioinformatics
... If we assume three possible states for every flexible dihedral angle in the backbone of a 100-residue protein, the number of possible backbone configurations is 3200. Even an incredibly fast computational or physical sampling in 10-15 s would mean that a complete sampling would take 1080 s, which ex ...
... If we assume three possible states for every flexible dihedral angle in the backbone of a 100-residue protein, the number of possible backbone configurations is 3200. Even an incredibly fast computational or physical sampling in 10-15 s would mean that a complete sampling would take 1080 s, which ex ...
Protein Structure - Laboratory of Molecular Modelling
... minimization (apply to proteins that have high sequence similarity to those in the database) • Threading = knowledge-based approach, given a structure database of interest (e.g. one that provides a limited set of possible structures per given sequence for fold recognition, one that provides a one st ...
... minimization (apply to proteins that have high sequence similarity to those in the database) • Threading = knowledge-based approach, given a structure database of interest (e.g. one that provides a limited set of possible structures per given sequence for fold recognition, one that provides a one st ...
Protein Structure
... minimization (apply to proteins that have high sequence similarity to those in the database) • Threading = knowledge-based approach, given a structure database of interest (e.g. one that provides a limited set of possible structures per given sequence for fold recognition, one that provides a one st ...
... minimization (apply to proteins that have high sequence similarity to those in the database) • Threading = knowledge-based approach, given a structure database of interest (e.g. one that provides a limited set of possible structures per given sequence for fold recognition, one that provides a one st ...
Diversity of proteins
... Covalent cross links ( R-S-S-R) and ionic interactions may stabilize some globular proteins ...
... Covalent cross links ( R-S-S-R) and ionic interactions may stabilize some globular proteins ...