The octamer binding factor Oct6: cDNA cloning and expression in
... of differential regulation of expression of the genetic content of the cell. That is, genes are turned on and off in response to intraor extra cellular cues. Regulation of gene expression can operate at the transcriptional and/or posttranscriptional level. Although some well documented examples of r ...
... of differential regulation of expression of the genetic content of the cell. That is, genes are turned on and off in response to intraor extra cellular cues. Regulation of gene expression can operate at the transcriptional and/or posttranscriptional level. Although some well documented examples of r ...
Protein Functional Annotation - Institute for Genome Sciences
... arisen due to a gene duplication within one species! –! paralogs will initially have the same function (just after the duplication) but as time goes by, one copy will be free to evolve new functions, as the other copy will maintain the original function. This process is called “neofunctionalization” ...
... arisen due to a gene duplication within one species! –! paralogs will initially have the same function (just after the duplication) but as time goes by, one copy will be free to evolve new functions, as the other copy will maintain the original function. This process is called “neofunctionalization” ...
Pierce Antibodies - Fisher Scientific
... Over 30,000 antibodies in 42 research areas. Thermo Scientific Pierce Primary Antibodies are developed for a wide variety of application needs. All of our antibodies are validated and guaranteed to perform in the stated application and species. Also, our Innovator’s Program rewards researchers for s ...
... Over 30,000 antibodies in 42 research areas. Thermo Scientific Pierce Primary Antibodies are developed for a wide variety of application needs. All of our antibodies are validated and guaranteed to perform in the stated application and species. Also, our Innovator’s Program rewards researchers for s ...
Protein and Glycoprotein Characterisation by Mass
... A novel final purification step involving fractional distillation using a source temperature gradient was all that was then required to produce the first successful sequence analysis of protein-derived peptides in 19682,3, in a study of the unknown sequence of silk fibroin (later found to be over1mi ...
... A novel final purification step involving fractional distillation using a source temperature gradient was all that was then required to produce the first successful sequence analysis of protein-derived peptides in 19682,3, in a study of the unknown sequence of silk fibroin (later found to be over1mi ...
View PDF - OMICS International
... Proteins are vital for health. They act like saviour of each cell by participating in all anabolic as well catabolic processes in the body. They are known to build the muscle mass and repair the damaged cells. However, the human body has limited capacity to store excess protein hence need regular su ...
... Proteins are vital for health. They act like saviour of each cell by participating in all anabolic as well catabolic processes in the body. They are known to build the muscle mass and repair the damaged cells. However, the human body has limited capacity to store excess protein hence need regular su ...
Mouse CNTF / Ciliary Neurotrophic Factor Protein (His Tag)
... biotinylated rat CNTFR-His (Cat:80019-R08B). The EC50 of biotinylated rat CNTFR-His (Cat:80019-R08B) is 5-12 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL. ...
... biotinylated rat CNTFR-His (Cat:80019-R08B). The EC50 of biotinylated rat CNTFR-His (Cat:80019-R08B) is 5-12 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL. ...
A green glow
... brain, thus giving an indication on cerebral development. GFP is also used to visualize something even smaller than cells: proteins. Several techniques have been developed to study their function. One such technique is used to find out where and when a particular protein is produced – in the brain, ...
... brain, thus giving an indication on cerebral development. GFP is also used to visualize something even smaller than cells: proteins. Several techniques have been developed to study their function. One such technique is used to find out where and when a particular protein is produced – in the brain, ...
INF380 – Proteomics
... characterization of posttranslational modifications, and comparison of proteins, we are primarily interested in attributes that can be of help in solving these tasks. For such attributes there exist some desirable requirements. – The value of the attribute should be (easily) measurable. – The attrib ...
... characterization of posttranslational modifications, and comparison of proteins, we are primarily interested in attributes that can be of help in solving these tasks. For such attributes there exist some desirable requirements. – The value of the attribute should be (easily) measurable. – The attrib ...
can detect white spot syndrome virus (wssv
... serious diseases in penaeid shrimp farming world wide. It is caused by white spot syndrome virus (WSSV). Mortality rates can reach 100% within 3-10 days after outbreaks begin. The first report was from Taiwan in 1992.1 Later the disease was reported from various parts of the world.2 WSSV infects not ...
... serious diseases in penaeid shrimp farming world wide. It is caused by white spot syndrome virus (WSSV). Mortality rates can reach 100% within 3-10 days after outbreaks begin. The first report was from Taiwan in 1992.1 Later the disease was reported from various parts of the world.2 WSSV infects not ...
Protein Unit Study Guide/Review Sheets
... If you have questions, make sure to ask them. Come after school for extra help. Review: these topics are not completely inclusive of test questions. You must be able to synthesize responses using this information and also to apply this information in different ways or contexts. GENERAL PROTEIN STRUC ...
... If you have questions, make sure to ask them. Come after school for extra help. Review: these topics are not completely inclusive of test questions. You must be able to synthesize responses using this information and also to apply this information in different ways or contexts. GENERAL PROTEIN STRUC ...
Nuclear gene expression 1
... Enhancers and Silencers 1. Enhancers stimulate transcription, while Silencers inhibit. 2. Orientation-independent – Flip 180 degrees, still work 3. Position-independent (mostly) – Can work at a distance from promoter core – Enhancers have been found all over 4. Bind regulatory transcription factors ...
... Enhancers and Silencers 1. Enhancers stimulate transcription, while Silencers inhibit. 2. Orientation-independent – Flip 180 degrees, still work 3. Position-independent (mostly) – Can work at a distance from promoter core – Enhancers have been found all over 4. Bind regulatory transcription factors ...
Bacterial enzymes that can deglycate glucose
... Amadori product of glucose-modified protein into fructosamine 3-phosphate [9]. Van Schaftingen and co-workers [7] cloned and fully characterized the enzyme, and reported that it had deglycating activity, resulting in 3-deoxyglucosone formation while regenerating the free lysine residue. Thus the pho ...
... Amadori product of glucose-modified protein into fructosamine 3-phosphate [9]. Van Schaftingen and co-workers [7] cloned and fully characterized the enzyme, and reported that it had deglycating activity, resulting in 3-deoxyglucosone formation while regenerating the free lysine residue. Thus the pho ...
Effects of rare codon clusters on high-level expression
... protein and frameshift fragment represented -5% and 2% o f total cell protein, respectively. In defined medium, total expression decreased to <1%, and production o f the frameshift fragment equaled that o f the full-length protein. Addition o f arginine to theminimal medium did not restore expressio ...
... protein and frameshift fragment represented -5% and 2% o f total cell protein, respectively. In defined medium, total expression decreased to <1%, and production o f the frameshift fragment equaled that o f the full-length protein. Addition o f arginine to theminimal medium did not restore expressio ...
Moonlighting proteins—an update
... proteins make use of the general methods seen in previously identified moonlighting proteins to switch between functions, although the details differ, for example how interacting with a different protein partner or cofactor results in a conformational change that then affects function. For some of the n ...
... proteins make use of the general methods seen in previously identified moonlighting proteins to switch between functions, although the details differ, for example how interacting with a different protein partner or cofactor results in a conformational change that then affects function. For some of the n ...
Protein Sulfenylation in Mitochondria: Biochemistry and
... they are very often evolutionary conserved pointing at a functional relevance of the cysteine at these positions. Cysteine oxidation processes serve many different purposes but in general two different functional roles can be distinguished (Fig. 1). In some cellular compartments such as in the bacte ...
... they are very often evolutionary conserved pointing at a functional relevance of the cysteine at these positions. Cysteine oxidation processes serve many different purposes but in general two different functional roles can be distinguished (Fig. 1). In some cellular compartments such as in the bacte ...
Title goes here
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
... Galton left his home and headed for a country fair… He believed that only a very few people had the characteristics necessary to keep societies healthy. He had devoted much of his career to measuring those characteristics, in fact, in order to prove that the vast majority of people did not have them ...
The Arabidopsis chloroplast ribosomal protein L21 is
... adapt the synthesis of the RPL21c gene to chloroplast development in mesophyll cells, in parallel with leaf development. As shown in Fig. 1, the mitochondrial RPL21m gene shows a different expression pattern compared to the chloroplast RPL21c gene. The RPL21c is preferentially expressed in photosynt ...
... adapt the synthesis of the RPL21c gene to chloroplast development in mesophyll cells, in parallel with leaf development. As shown in Fig. 1, the mitochondrial RPL21m gene shows a different expression pattern compared to the chloroplast RPL21c gene. The RPL21c is preferentially expressed in photosynt ...
Overview of Protein Structure • The three
... Proteins are only marginally stable at physiological temperature, and they are held together by a number of weak, interdependent forces. The thermodynamic hypothesis of protein stability states that the native conformation of a protein is thermodynamically the most stable conformation that a polypep ...
... Proteins are only marginally stable at physiological temperature, and they are held together by a number of weak, interdependent forces. The thermodynamic hypothesis of protein stability states that the native conformation of a protein is thermodynamically the most stable conformation that a polypep ...
Silver PA, Brent R, Ptashne M. DNA binding is not
... We do not know how much LexA or LexA-GAL4 is actually in the nucleus. However, since both proteins are able to function as transcriptional regulatory proteins, some must gain access to the interior of the nucleus and recognize the LexA operator. Because of its larger size, one might not expect LexA- ...
... We do not know how much LexA or LexA-GAL4 is actually in the nucleus. However, since both proteins are able to function as transcriptional regulatory proteins, some must gain access to the interior of the nucleus and recognize the LexA operator. Because of its larger size, one might not expect LexA- ...
From DNA to Protein: Transcription and Translation
... •A tRNA with an anticodon complementary to the mRNA codon pairs up, carrying the corresponding amino acid. •A second tRNA pairs with the next codon, and the two amino acids are joined by a peptide bond. •The mRNA is slide down the ribosome bringing the next codon to the tRNA binding site. •This pro ...
... •A tRNA with an anticodon complementary to the mRNA codon pairs up, carrying the corresponding amino acid. •A second tRNA pairs with the next codon, and the two amino acids are joined by a peptide bond. •The mRNA is slide down the ribosome bringing the next codon to the tRNA binding site. •This pro ...
Presentation (PowerPoint File)
... DNA (nucleotides, 4 types): information carrier/encoder RNA: bridge from DNA to protein Protein (amino acids, 20 types): action molecules. ...
... DNA (nucleotides, 4 types): information carrier/encoder RNA: bridge from DNA to protein Protein (amino acids, 20 types): action molecules. ...
Advances in selectable marker genes for plant transformation
... regenerate fully developed plants from the transformed cells. Selectable marker genes are pivotal for the development of plant transformation technologies because marker genes allow researchers to identify or isolate the cells that are expressing the cloned DNA, to monitor and select the transformed ...
... regenerate fully developed plants from the transformed cells. Selectable marker genes are pivotal for the development of plant transformation technologies because marker genes allow researchers to identify or isolate the cells that are expressing the cloned DNA, to monitor and select the transformed ...
Proteins
... complex when the R groups of amino acids far apart in the primary structure bond with one another. • This level of structure is called tertiary structure and is the highest level of structure that an individual polypeptide can attain. • However, many proteins require more than one chain to function. ...
... complex when the R groups of amino acids far apart in the primary structure bond with one another. • This level of structure is called tertiary structure and is the highest level of structure that an individual polypeptide can attain. • However, many proteins require more than one chain to function. ...
Characterization of the ves Gene, Which is Expressed at a Low
... upstream of the mRNA start site like the UP elements extensively examined (Ross et al., 1998). A sequence, UAUGCGUAAAAUGUCGG, similar to those of downstream boxes in cold-shock proteins is located at positions 351 to 367, and the matching number in this sequence to the 16S rRNA sequence (GC + AU + G ...
... upstream of the mRNA start site like the UP elements extensively examined (Ross et al., 1998). A sequence, UAUGCGUAAAAUGUCGG, similar to those of downstream boxes in cold-shock proteins is located at positions 351 to 367, and the matching number in this sequence to the 16S rRNA sequence (GC + AU + G ...
Application of SVM to predict membrane protein types
... As is well known, the independent data set test, subsampling test and jackknife test are the three methods often used for cross-validation in statistical prediction. Among these three, however, the jackknife test is deemed as the most effective and objective one; see, e.g. Chou and Zhang (1995) for ...
... As is well known, the independent data set test, subsampling test and jackknife test are the three methods often used for cross-validation in statistical prediction. Among these three, however, the jackknife test is deemed as the most effective and objective one; see, e.g. Chou and Zhang (1995) for ...