A Drosophila Third Chromosome Minute Locus Encodes
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
Predicting functional linkages from gene fusions with
... Stone linkages. Each of the two component regulator proteins is linked with each of the two component sensor proteins because of the presence in the sequence database of a single Rosetta Stone protein from Synechocystis (GenBank identifier 1653468) that encodes fused regulator and sensor domains. As ...
... Stone linkages. Each of the two component regulator proteins is linked with each of the two component sensor proteins because of the presence in the sequence database of a single Rosetta Stone protein from Synechocystis (GenBank identifier 1653468) that encodes fused regulator and sensor domains. As ...
A biochemical portrait of the nidovirus RNA polymerases and helicase
... two (or more) active sites are positioned opposite each other. A striking demonstration of these consequences was visualised in fluorescence resonance energy transfer (FRET) studies of the HIV-1 RT [173,174]. This enzyme needs to catalyse a multi-step process, including i) RNA-templated minus strand ...
... two (or more) active sites are positioned opposite each other. A striking demonstration of these consequences was visualised in fluorescence resonance energy transfer (FRET) studies of the HIV-1 RT [173,174]. This enzyme needs to catalyse a multi-step process, including i) RNA-templated minus strand ...
and Mountain Lions (Puma concolor) in North America
... using the genetic algorithm for recombination detection (GARD), with a general discrete distribution of sites into two rate classes (23). Breakpoints identified by improved Akaike information criterion (AIC) values were considered significant only if subsequently supported by a Kishino-Hasegawa test ...
... using the genetic algorithm for recombination detection (GARD), with a general discrete distribution of sites into two rate classes (23). Breakpoints identified by improved Akaike information criterion (AIC) values were considered significant only if subsequently supported by a Kishino-Hasegawa test ...
90718 Internal v2 3.6 A2 Generic 2006
... DNA profiling is being used as part of an international research project to identify twenty thousand marine fish species by the year 2010. Scientists will identify the sequence of bases in a specific region of mitochondrial DNA which does not show much variation within species, but between species i ...
... DNA profiling is being used as part of an international research project to identify twenty thousand marine fish species by the year 2010. Scientists will identify the sequence of bases in a specific region of mitochondrial DNA which does not show much variation within species, but between species i ...
Tps1 regulates the pentose phosphate pathway, nitrogen
... mutant in vivo. We subcloned the 8 kb PstI fragment into the sulphonylurea-carrying vector pCB1532 (Carroll et al., 1994) to give pRAW8, and removed a 2 kb SmaI fragment to give pRAW9. To facilitate site directed mutagenesis, a 2.8 kb NotI and NdeI fragment was digested from pRAW9 and subcloned int ...
... mutant in vivo. We subcloned the 8 kb PstI fragment into the sulphonylurea-carrying vector pCB1532 (Carroll et al., 1994) to give pRAW8, and removed a 2 kb SmaI fragment to give pRAW9. To facilitate site directed mutagenesis, a 2.8 kb NotI and NdeI fragment was digested from pRAW9 and subcloned int ...
Report of euro-ATAXIA research conference (3 November 2012) By
... ways. He described next generation sequencing as a technological advance that will help in providing patients with specific diagnoses and highlighted how many new types of recessive ataxia are emerging, often very rare forms. Some newer conditions were described in some more detail. ARCA1, for examp ...
... ways. He described next generation sequencing as a technological advance that will help in providing patients with specific diagnoses and highlighted how many new types of recessive ataxia are emerging, often very rare forms. Some newer conditions were described in some more detail. ARCA1, for examp ...
Microarray on Germinating Yeast Spores (WP2)
... The experimental outline • Diploid cells were sporulated in 1% KAc • The spores were left resting at 4C for at least 14 days in 0.5% TritonX-100 solution • Spores were then put to rich nutrient growth medium containing 2% glucose • Samples for total RNA extraction were taken in a logarithmic timefa ...
... The experimental outline • Diploid cells were sporulated in 1% KAc • The spores were left resting at 4C for at least 14 days in 0.5% TritonX-100 solution • Spores were then put to rich nutrient growth medium containing 2% glucose • Samples for total RNA extraction were taken in a logarithmic timefa ...
Sequence and transcription analysis of the Petunia mitochondrial
... nor anther tissues (lanes 2,5) nor in mitochondrial RNA preparations from suspension cultures (lane 7 ) . The 3mall overall increase in protection by ovary RNA (lane 6) is probably due to a higher proportion of mtRNA to total RNA, since thi3 phenomenon is seen with other genes analyzed^. To distingu ...
... nor anther tissues (lanes 2,5) nor in mitochondrial RNA preparations from suspension cultures (lane 7 ) . The 3mall overall increase in protection by ovary RNA (lane 6) is probably due to a higher proportion of mtRNA to total RNA, since thi3 phenomenon is seen with other genes analyzed^. To distingu ...
w0405_tutorial13
... Given a genome with a set of genes. Recall that each gene is represented by a probe (a short segment) of the corresponding cDNA. The length of a probe can be 20, 50, 70! The probe design problem tries to identify a probe for every gene. ...
... Given a genome with a set of genes. Recall that each gene is represented by a probe (a short segment) of the corresponding cDNA. The length of a probe can be 20, 50, 70! The probe design problem tries to identify a probe for every gene. ...
A conserved enhancer of the human and murine Hoxa
... from the endogenous Hoxa-7 gene during the period of development when Hoxa-7 expression is established (Püschel et al., 1990). Furthermore, at least three distinct elements responsible for different levels of control, being active at different stages of development, were identified (Püschel et al., ...
... from the endogenous Hoxa-7 gene during the period of development when Hoxa-7 expression is established (Püschel et al., 1990). Furthermore, at least three distinct elements responsible for different levels of control, being active at different stages of development, were identified (Püschel et al., ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... PSB constitute the order Chromatiales, and comprise the Chromatiaceae and Ectothiorhodospiraceae families, which can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phyloge ...
... PSB constitute the order Chromatiales, and comprise the Chromatiaceae and Ectothiorhodospiraceae families, which can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phyloge ...
A novel atlas of gene expression in human skeletal
... We selected a subset of 361 arrays from the compendium to study the effect of aging on gene expression, i.e., 211 arrays from the HG-133A array and 150 from the HGU133 + 2 array that had specific annotation of age and gender, ranging from <1 year up to 83-year-old individuals (Figure S2 in Additiona ...
... We selected a subset of 361 arrays from the compendium to study the effect of aging on gene expression, i.e., 211 arrays from the HG-133A array and 150 from the HGU133 + 2 array that had specific annotation of age and gender, ranging from <1 year up to 83-year-old individuals (Figure S2 in Additiona ...
Two Closely Related Human Members of Chitinase
... PC12 cells gave the sustained activation together with the nuclear translocation of ERK1/2, which led to the neuronal differentiation, while EGF gave short activation of ERK1/2 without change in cellular localization, resulted in a proliferative signal [2]. Plenty of ligands provoked significantly s ...
... PC12 cells gave the sustained activation together with the nuclear translocation of ERK1/2, which led to the neuronal differentiation, while EGF gave short activation of ERK1/2 without change in cellular localization, resulted in a proliferative signal [2]. Plenty of ligands provoked significantly s ...
A picorna-like virus from the red imported fire ant, Solenopsis invicta
... Fig. 2. Comparisons of predicted amino acid sequences of nonstructural and structural proteins of SINV-1, picorna-like viruses (ABPV, SBV, BQCV), and viruses representative of the Picornaviridae (HAV) and Comoviridae (CPMV). Alignments are of the conserved regions of the putative helicase (A), cyste ...
... Fig. 2. Comparisons of predicted amino acid sequences of nonstructural and structural proteins of SINV-1, picorna-like viruses (ABPV, SBV, BQCV), and viruses representative of the Picornaviridae (HAV) and Comoviridae (CPMV). Alignments are of the conserved regions of the putative helicase (A), cyste ...
Gene Section GRPR (Gastrin-Releasing Peptide Receptor) Atlas of Genetics and Cytogenetics
... chromosome and the GRP-R has 7 transmembrane (TM) domains, an extracellular N-terminal and intracellular C-terminal. Exon 1 codes for TM 1, 2 and 3 domains with splice site in IC loop 2 (Asp137). Exon 2 codes for TM 4 and 5 with a Gln255 splice site. Exon 3 codes for TM 6-7 domains and the cytoplasm ...
... chromosome and the GRP-R has 7 transmembrane (TM) domains, an extracellular N-terminal and intracellular C-terminal. Exon 1 codes for TM 1, 2 and 3 domains with splice site in IC loop 2 (Asp137). Exon 2 codes for TM 4 and 5 with a Gln255 splice site. Exon 3 codes for TM 6-7 domains and the cytoplasm ...
DEFINITIONS - Microbiology Book
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
A transcription factor of lipid synthesis, sterol regulatory element
... N-terminal transactivation domain was deleted (DTA– SREBP-1a) [12], SREBP-1a-induced G1 arrest was abolished. Its action was also cancelled by the introduction of a point mutation (YR–SREBP-1a) through which SREBP-1 loses its ability to bind to an SRE, which is generally found in promoters of known ...
... N-terminal transactivation domain was deleted (DTA– SREBP-1a) [12], SREBP-1a-induced G1 arrest was abolished. Its action was also cancelled by the introduction of a point mutation (YR–SREBP-1a) through which SREBP-1 loses its ability to bind to an SRE, which is generally found in promoters of known ...
Molecular and Phylogenetic Characterization of Cytokine Genes
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
Isolation and characterization of cDNA clones encoding
... clones analyzed contained the 138 amino acid coding regions of their respective mature proteins, but only partial sequences of the signal peptides. Minor differences between the nucleotide sequences for clones belonging to the same class were detected. Comparison of the amino acid sequence for PR-la ...
... clones analyzed contained the 138 amino acid coding regions of their respective mature proteins, but only partial sequences of the signal peptides. Minor differences between the nucleotide sequences for clones belonging to the same class were detected. Comparison of the amino acid sequence for PR-la ...
701KB - NZQA
... phenotypes _of various traits in genetically identical organisms. Armadillos are ideal animals to use in such research, because they are born as quadmplets derived from a single fertilised egg. This means that all four armadillo pups share the same genetic sequence. In a number of experiments carrie ...
... phenotypes _of various traits in genetically identical organisms. Armadillos are ideal animals to use in such research, because they are born as quadmplets derived from a single fertilised egg. This means that all four armadillo pups share the same genetic sequence. In a number of experiments carrie ...
Chapter 2 - Chemical Engineering - Michigan Technological University
... Single or multi-celled organisms that contain chloroplasts and perform photosynthesis. Size is 10 - 30 µm. Diatoms contain silica in their cell walls and are used as filter aids in industry. Some algae are used in the wastewater treatment industry with simultaneous production of single-cell protein. ...
... Single or multi-celled organisms that contain chloroplasts and perform photosynthesis. Size is 10 - 30 µm. Diatoms contain silica in their cell walls and are used as filter aids in industry. Some algae are used in the wastewater treatment industry with simultaneous production of single-cell protein. ...
Amino Acid Differences in the Deduced 5
... shown in Figure 1. Because of the expression variability, the results of the activity assays had to be normalized to 5-LOX protein, as determined by densitometry of 5-LOX immunoblotting. To obtain reliable activity data, 3 clones were randomly selected for wt 5-LOX and for each mutant, and activity ...
... shown in Figure 1. Because of the expression variability, the results of the activity assays had to be normalized to 5-LOX protein, as determined by densitometry of 5-LOX immunoblotting. To obtain reliable activity data, 3 clones were randomly selected for wt 5-LOX and for each mutant, and activity ...
Innate immune detection of microbial nucleic acids
... Structural basis of self versus non-self RNA discrimination by RLRs It was unclear for some time how RLRs bind their ligands because none of the helicases contain a classical RNAbinding motif. However, recent studies solving the crystal structure of both RIG-I and MDA5 in complex with RNA ligands ha ...
... Structural basis of self versus non-self RNA discrimination by RLRs It was unclear for some time how RLRs bind their ligands because none of the helicases contain a classical RNAbinding motif. However, recent studies solving the crystal structure of both RIG-I and MDA5 in complex with RNA ligands ha ...
Transfer of genetic material between the chloroplast and nucleus
... The question, however, remains: is the reason for this that these sequences are more often inserted, less often removed or a combination of both? Matsou et al. (2005) using the rice nuclear genome data reported that the plant nuclear genome is in equilibrium between frequent integration and rapid el ...
... The question, however, remains: is the reason for this that these sequences are more often inserted, less often removed or a combination of both? Matsou et al. (2005) using the rice nuclear genome data reported that the plant nuclear genome is in equilibrium between frequent integration and rapid el ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.