Cas9 Nuclease NLS, S. pyogenes
... Disclaimer: The information provided on this Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guid ...
... Disclaimer: The information provided on this Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guid ...
shaping our food
... example noticeable in traits related to disease resistance in animals and to biomass in plants. A decade after the discovery of heterosis, the fact that many traits depend on many genes, so called quantitative traits, was understood and statistical models were developed to account for such traits in ...
... example noticeable in traits related to disease resistance in animals and to biomass in plants. A decade after the discovery of heterosis, the fact that many traits depend on many genes, so called quantitative traits, was understood and statistical models were developed to account for such traits in ...
Phylogenetic analysis of phytoplasmas based on sequences
... Previous analysis based primarily on the 16S rDNA gene has indicated that the aster yellows group phytoplasmas (16SrI) form a distinct subclade within the phytoplasma clade, and further analyses of this subclade using genes such as secY and tuf have subdivided this group into distinct lineages (Lee ...
... Previous analysis based primarily on the 16S rDNA gene has indicated that the aster yellows group phytoplasmas (16SrI) form a distinct subclade within the phytoplasma clade, and further analyses of this subclade using genes such as secY and tuf have subdivided this group into distinct lineages (Lee ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
... genome (P , 1e240). These are presumably orthologs that diverged from a common ancestor of E. coli and S. typhi, although it is also possible that a few of these sequences are lateral transfer events between these two lineages since their divergence. The latter events would be characterized by excep ...
... genome (P , 1e240). These are presumably orthologs that diverged from a common ancestor of E. coli and S. typhi, although it is also possible that a few of these sequences are lateral transfer events between these two lineages since their divergence. The latter events would be characterized by excep ...
PMC-AT Enzyme Engineering Research Overview.
... The actual processes would depend on what the mutations are and how many mutants are to be made. By synthetic gene method – Work with gene synthesis group to construct synthetic constructs, esp. in how to set up efficient oligonucleotides coverage for all the mutations. Clone all mutant constructs i ...
... The actual processes would depend on what the mutations are and how many mutants are to be made. By synthetic gene method – Work with gene synthesis group to construct synthetic constructs, esp. in how to set up efficient oligonucleotides coverage for all the mutations. Clone all mutant constructs i ...
The Organellar Genome and Metabolic Potential
... of genes and proteins have been lost, gained from various sources, or retargeted to other organelles, thus shaping the huge diversity of current mitochondria and their homologs in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mito ...
... of genes and proteins have been lost, gained from various sources, or retargeted to other organelles, thus shaping the huge diversity of current mitochondria and their homologs in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mito ...
Luciferase Reporter Vector Protocol
... factors, all of which are also covered by our Protein/DNA Arrays. See www.panomics.com for an up-to-date list of all of the Luciferase Reporter Vectors Principle of Luciferase Reporter Vectors The Luciferase Reporter Vectors have been specially constructed to report the binding activity of an indivi ...
... factors, all of which are also covered by our Protein/DNA Arrays. See www.panomics.com for an up-to-date list of all of the Luciferase Reporter Vectors Principle of Luciferase Reporter Vectors The Luciferase Reporter Vectors have been specially constructed to report the binding activity of an indivi ...
Candidate genes and single nucleotide polymorphisms associated
... the GHR gene and was also associated with a significant effect on the tertiary structure of the GHR protein (P < 0.05) as modeled using SWISSModel software. Least square means for each genotype were estimated and an over-dominance effect was observed for the SNP located in the GHR, CAST, ACAD11 and ...
... the GHR gene and was also associated with a significant effect on the tertiary structure of the GHR protein (P < 0.05) as modeled using SWISSModel software. Least square means for each genotype were estimated and an over-dominance effect was observed for the SNP located in the GHR, CAST, ACAD11 and ...
On the trail of protein sequences
... a number of computer aspects and included some newly identified homologies. Unhappily, there was a 6-year delay between submission and publication (Doolittle, 1979), during which time others had come to many of the same conclusions. Some of the predictions of homology made in the article have only r ...
... a number of computer aspects and included some newly identified homologies. Unhappily, there was a 6-year delay between submission and publication (Doolittle, 1979), during which time others had come to many of the same conclusions. Some of the predictions of homology made in the article have only r ...
PDF - International Journal of Medical Sciences
... variation and immune evasion. However, in our previous studies, proteins MPT64, PstS1, Rv0309 and Rv2945c all harbored higher numbers of amino acid substitutions in their T cell epitopes, which suggests their roles in ongoing immune evasion. Here, we used the same set of 180 clinical M. tuberculosis ...
... variation and immune evasion. However, in our previous studies, proteins MPT64, PstS1, Rv0309 and Rv2945c all harbored higher numbers of amino acid substitutions in their T cell epitopes, which suggests their roles in ongoing immune evasion. Here, we used the same set of 180 clinical M. tuberculosis ...
Human Genetics
... • Human genome information has tremendous potential for the entire globe • World organizations are discussing how nations can share new diagnostic tests and therapeutics • Individual nations are adopting guidelines to use genetic information to suit their strengths ...
... • Human genome information has tremendous potential for the entire globe • World organizations are discussing how nations can share new diagnostic tests and therapeutics • Individual nations are adopting guidelines to use genetic information to suit their strengths ...
Two fatty acid ∆9-desaturase genes, ole1 and ole2
... ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ∆9-1 gene encoded a protein of 445 aa which shared 99 % identity with the LM9 gene product. These proteins also showed 40–60 % identity to the ∆9desa ...
... ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ∆9-1 gene encoded a protein of 445 aa which shared 99 % identity with the LM9 gene product. These proteins also showed 40–60 % identity to the ∆9desa ...
Gene Section SDHB (succinate dehydrogenase complex II,
... fumarate + ubiquinol) and carries electrons from FADH to CoQ. It is composed of four nuclear-encoded subunits. The subunit B protein or iron-sulfur protein, which binds three different iron-sulfur clusters, is directly involved in the catalytic activity of succinate dehydrogenase. ...
... fumarate + ubiquinol) and carries electrons from FADH to CoQ. It is composed of four nuclear-encoded subunits. The subunit B protein or iron-sulfur protein, which binds three different iron-sulfur clusters, is directly involved in the catalytic activity of succinate dehydrogenase. ...
Z. Naturforsch. 66c
... probability for a successful establishment of HGT (Nielsen et al., 1998). Until now the cultivation of GM plants in the field has not revealed any evidence that transgenes have been transferred to bacteria (Gebhard and Smalla, 1998; Paget et al., 1998; Badosa et al., 2004; Demanèche et al., 2008; Wag ...
... probability for a successful establishment of HGT (Nielsen et al., 1998). Until now the cultivation of GM plants in the field has not revealed any evidence that transgenes have been transferred to bacteria (Gebhard and Smalla, 1998; Paget et al., 1998; Badosa et al., 2004; Demanèche et al., 2008; Wag ...
Identification of Pseudomonas proteins coordinately
... when supplied as the sole source of carbon and nitrogen (Sonawane et al., 2003). All of these amino acids strongly and specifically induce periplasmic glutaminase/asparaginase (PGA) (Hüser et al., 1999). On the other hand, PGA is subject to carbon catabolite repression by glucose and dicarboxylic a ...
... when supplied as the sole source of carbon and nitrogen (Sonawane et al., 2003). All of these amino acids strongly and specifically induce periplasmic glutaminase/asparaginase (PGA) (Hüser et al., 1999). On the other hand, PGA is subject to carbon catabolite repression by glucose and dicarboxylic a ...
NRT2 - Clark University
... H.velutipes clade has, in fact, been demonstrated (Aanen, 2001). ∆ Problems with these hypotheses include the likelihood of these events being of some debate, and also the lack of additional corroborative molecular evidence such as additional ITS sequences in H. helodes. These are not necessarily fa ...
... H.velutipes clade has, in fact, been demonstrated (Aanen, 2001). ∆ Problems with these hypotheses include the likelihood of these events being of some debate, and also the lack of additional corroborative molecular evidence such as additional ITS sequences in H. helodes. These are not necessarily fa ...
Oncometabolites: tailoring our genes
... and modulation of the epigenome. They include enzymes that are involved in regulating gene expression via DNA and histone tail demethylation. In this review, we will focus on the link between metabolism and epigenetics, and how we may target oncometabolite-induced tumorigenesis in the future. ...
... and modulation of the epigenome. They include enzymes that are involved in regulating gene expression via DNA and histone tail demethylation. In this review, we will focus on the link between metabolism and epigenetics, and how we may target oncometabolite-induced tumorigenesis in the future. ...
innate, adaptive and intrinsic immunity in human immunodeficiency
... dendritic cells partially restores impaired antigen specific CD4+T cell responses associated with HIV-1 infection (Donaghy et al., 2001). Thus there is a belief emanating from these studies that immunization strategies, which target dendritic cells and other antigen presenting cells such as monocyte ...
... dendritic cells partially restores impaired antigen specific CD4+T cell responses associated with HIV-1 infection (Donaghy et al., 2001). Thus there is a belief emanating from these studies that immunization strategies, which target dendritic cells and other antigen presenting cells such as monocyte ...
Genetic Mutations SDK Nov 2, 2012
... Give examples of deletions, duplications, and insertions in genes Define trinucleotide repeat expansions and how they cause neurological diseases ...
... Give examples of deletions, duplications, and insertions in genes Define trinucleotide repeat expansions and how they cause neurological diseases ...
Proc. Natl. Acad. Sci. USA 96, 4718-4723.
... Proc. Natl. Acad. Sci. USA 96 (1999) AtCBL3. The AtCBL2 and AtCBL3 cDNAs are both 1,043 bp long and contain ORFs of 216 aa each, corresponding to potential polypeptides of 25.8 kDa. As shown in Fig. 1B, the amino acid sequences of AtCBL proteins are highly conserved (63% identity and 79% similarity ...
... Proc. Natl. Acad. Sci. USA 96 (1999) AtCBL3. The AtCBL2 and AtCBL3 cDNAs are both 1,043 bp long and contain ORFs of 216 aa each, corresponding to potential polypeptides of 25.8 kDa. As shown in Fig. 1B, the amino acid sequences of AtCBL proteins are highly conserved (63% identity and 79% similarity ...
Bacterial Regulation
... (a) Lactose present, glucose scarce (cAMP level high): abundant lac mRNA synthesized. If glucose is scarce, the high level of cAMP activates CAP, and the lac operon produces large amounts of mRNA for the lactose pathway. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
... (a) Lactose present, glucose scarce (cAMP level high): abundant lac mRNA synthesized. If glucose is scarce, the high level of cAMP activates CAP, and the lac operon produces large amounts of mRNA for the lactose pathway. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
Multiple Sequence Alignment
... Alignment of two sequences which has maximum base matches and minimum gap by using the algorithm of Needleman and Wunsch. ...
... Alignment of two sequences which has maximum base matches and minimum gap by using the algorithm of Needleman and Wunsch. ...
Evolutionary molecular genetic clocks—a perpetual exercise in
... this discrepancy is that the “intra-cellular role of scavenging oxygen radicals would seem likely to have remained the same through time and across lineages over the last 650 My [Ma]”. His conclusion is “that we are left with no predictive power and no clock proper”. Another significant problem is t ...
... this discrepancy is that the “intra-cellular role of scavenging oxygen radicals would seem likely to have remained the same through time and across lineages over the last 650 My [Ma]”. His conclusion is “that we are left with no predictive power and no clock proper”. Another significant problem is t ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.