Transcription & Translation
... Messenger RNA (mRNA) – carries the genetic information (codons) from DNA Transfer RNAs (tRNAs) – carries amino acids contains anti-codon Ribosomal RNA (rRNA) – a structural component of ribosomes ...
... Messenger RNA (mRNA) – carries the genetic information (codons) from DNA Transfer RNAs (tRNAs) – carries amino acids contains anti-codon Ribosomal RNA (rRNA) – a structural component of ribosomes ...
Supplemental Material 1 Simultaneous isolation of mRNA, miRNA
... target islands are penalized because they may introduce spurious predictions. Second, it rewards instances that maximize the minimum distance between the same MREs. The MREs that are targeted by miR-n must be spaced out enough that miR-n+RISC can sit on the mRNA target without steric interference by ...
... target islands are penalized because they may introduce spurious predictions. Second, it rewards instances that maximize the minimum distance between the same MREs. The MREs that are targeted by miR-n must be spaced out enough that miR-n+RISC can sit on the mRNA target without steric interference by ...
Bacterial transformation
... bacteria cost little to maintain and are quick and easy to grow in the lab. However, in order for the bacteria to copy the DNA it needs to be circular, contain a bacterial origin of replication (ori), and contain a selectable marker in addition to your piece of DNA (often generically referred to as ...
... bacteria cost little to maintain and are quick and easy to grow in the lab. However, in order for the bacteria to copy the DNA it needs to be circular, contain a bacterial origin of replication (ori), and contain a selectable marker in addition to your piece of DNA (often generically referred to as ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... 0.5 or 1 ml of acetate solution (NH4-acetate/Mg-acetate; 2.5 g each/100 ml). The final acetate concentration of the medium was 0.05%. Depending on the species, NaCl was added to the medium to a final concentration of 0–10% (medium for marine isolates contained 2% NaCl). After inoculation, the bottle ...
... 0.5 or 1 ml of acetate solution (NH4-acetate/Mg-acetate; 2.5 g each/100 ml). The final acetate concentration of the medium was 0.05%. Depending on the species, NaCl was added to the medium to a final concentration of 0–10% (medium for marine isolates contained 2% NaCl). After inoculation, the bottle ...
Editing the Mushroom
... THE TELLTALE SIGN of any transformational technology is how quickly researchers apply it to their own scientific problems. By that standard, CRISPR ranks among the most powerful additions to biology’s tool kit in the past half a century. The geneedited mushroom is a case in point. Yinong Yang—his fi ...
... THE TELLTALE SIGN of any transformational technology is how quickly researchers apply it to their own scientific problems. By that standard, CRISPR ranks among the most powerful additions to biology’s tool kit in the past half a century. The geneedited mushroom is a case in point. Yinong Yang—his fi ...
A Genetic Link Between an mRNA-Specific Translational
... To check that the mutant cells had remained rho+, aliquots of the mutant cultures grown at nonpermissive temperature were diluted and plated on YPD for single colonies. The colonies were then mated to a Pet+, rho" tester strain and the ability of the resulting diploids to grow on YPEG medium was sco ...
... To check that the mutant cells had remained rho+, aliquots of the mutant cultures grown at nonpermissive temperature were diluted and plated on YPD for single colonies. The colonies were then mated to a Pet+, rho" tester strain and the ability of the resulting diploids to grow on YPEG medium was sco ...
transcription
... • Most genes do not simply stay “on,” but instead are transcribed in accordance with the needs of an organism. ...
... • Most genes do not simply stay “on,” but instead are transcribed in accordance with the needs of an organism. ...
Protocol S1.
... The accumulation of GM-dipeptide in the peptidoglycan of H. pylori during the transition from spiral into coccoid forms might be a consequence of an increase in the cytoplasm of the amount of PG precursors carrying a dipeptide (ultimately incorporated in periplasmic PG) or carboxy/endopeptidase acti ...
... The accumulation of GM-dipeptide in the peptidoglycan of H. pylori during the transition from spiral into coccoid forms might be a consequence of an increase in the cytoplasm of the amount of PG precursors carrying a dipeptide (ultimately incorporated in periplasmic PG) or carboxy/endopeptidase acti ...
Prokaryotic DNA organization • Circular DNA • Condensed by packaging proteins
... Polymerase chain reaction (PCR) • Requires DNA polymerase that is not inactivated by high temperatures • Taq, Vent polymerases isolated from thermophiles Fig,14.8 ...
... Polymerase chain reaction (PCR) • Requires DNA polymerase that is not inactivated by high temperatures • Taq, Vent polymerases isolated from thermophiles Fig,14.8 ...
CRONOS: the cross-reference navigation server
... In order to detect gene and protein names which are assigned to products of different genes and thus result in erroneous cross-references, dedicated lists are created for each organism separately. Organism-specific lists are necessary, since terms that are ambiguous in one organism might be explicit ...
... In order to detect gene and protein names which are assigned to products of different genes and thus result in erroneous cross-references, dedicated lists are created for each organism separately. Organism-specific lists are necessary, since terms that are ambiguous in one organism might be explicit ...
Varicella-Zoster Virus Specifies a Thymidylate Synthetase
... The inhibitor 5-fluoro-2'-deoxyuridylate (FdUMP) has been used successfully to identify the TS protein in crude extracts of human cells (Lockshin et aL, 1979) and mouse cells (Ayusawa et al., 1981) and, by virtue of the difference in molecular weight between the cellular and viral proteins, the HVS ...
... The inhibitor 5-fluoro-2'-deoxyuridylate (FdUMP) has been used successfully to identify the TS protein in crude extracts of human cells (Lockshin et aL, 1979) and mouse cells (Ayusawa et al., 1981) and, by virtue of the difference in molecular weight between the cellular and viral proteins, the HVS ...
Sytenol®A
... Sytenol®A for Skin Protection Extended life-span, more spare time and excessive exposure to UV radiation from sunlight or tanning devices, especially in the Western population, has resulted in an ever increasing demand to protect human skin against the detrimental effects of UV-exposure. It is well ...
... Sytenol®A for Skin Protection Extended life-span, more spare time and excessive exposure to UV radiation from sunlight or tanning devices, especially in the Western population, has resulted in an ever increasing demand to protect human skin against the detrimental effects of UV-exposure. It is well ...
The Three Domains of Life:
... obscure genomic sequences. The criteria being used for these analyses were: 1. The protein must be functional for the whole cell. Thus, with very few exceptions, we excluded putative sequences as well as proteins from mitochondria or chloroplasts. 2. Diverse proteins were selected with at least 4 sp ...
... obscure genomic sequences. The criteria being used for these analyses were: 1. The protein must be functional for the whole cell. Thus, with very few exceptions, we excluded putative sequences as well as proteins from mitochondria or chloroplasts. 2. Diverse proteins were selected with at least 4 sp ...
Chpt10_TxnRNAPol.doc
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
See more scientific discussion on this here.
... effect. It was not selected against by Natural Selection. What other explanation could there be? It is also a definite piece of evidence for evolution in the sense just described but also because this non-functional gene has the same missing parts in all of the anthropoid primates. ...
... effect. It was not selected against by Natural Selection. What other explanation could there be? It is also a definite piece of evidence for evolution in the sense just described but also because this non-functional gene has the same missing parts in all of the anthropoid primates. ...
Latent Semantic Analysis of the Languages of Life
... setting the majority of the singular values to zero and keeping only the few influential singular values. The latter approach allows us to create a reduced space from which words used in the same context with one another are represented close together. ...
... setting the majority of the singular values to zero and keeping only the few influential singular values. The latter approach allows us to create a reduced space from which words used in the same context with one another are represented close together. ...
1. Introduction - diss.fu
... Transposons play a special role in bacterial evolution because of their ability to move between the chromosome and various plasmid and integrated phage DNA. They vary in size, structure, and the way they move. Generally, bacterial transposons can be classified into four groups: the first is the IS s ...
... Transposons play a special role in bacterial evolution because of their ability to move between the chromosome and various plasmid and integrated phage DNA. They vary in size, structure, and the way they move. Generally, bacterial transposons can be classified into four groups: the first is the IS s ...
Evaluation and Comparison of the GUS, LUC and GFP Reporter
... a bright green fluorescence when folded correctly and illuminated with UV or blue light (Fig. 1 D; Chalfie et al., 1994). For effective expression in plants, the GFP coding sequence was adapted to remove regions with high AT content (cryptic intron sequences and splice sites) and a cryptic nuclear i ...
... a bright green fluorescence when folded correctly and illuminated with UV or blue light (Fig. 1 D; Chalfie et al., 1994). For effective expression in plants, the GFP coding sequence was adapted to remove regions with high AT content (cryptic intron sequences and splice sites) and a cryptic nuclear i ...
Recombinant DNA technology
... When bacteria take up a plasmid, we say they have been transformed Bacteria are encouraged to take up foreign DNA by: 1.heat-shock the bacteria at 42 C. followed by placing them on ice. 2. Place them in an electric field “electroporation” Then selection through selectable markers on the plasmid. ...
... When bacteria take up a plasmid, we say they have been transformed Bacteria are encouraged to take up foreign DNA by: 1.heat-shock the bacteria at 42 C. followed by placing them on ice. 2. Place them in an electric field “electroporation” Then selection through selectable markers on the plasmid. ...
Nature Methods
... thereby creating MacQ-mOrange2 and MacQ-mCitrine. This strategy combines the fast kinetic properties of the rhodopsin with the brightness conferred by the fluorescent proteins. On the other hand, Lin’s ASAP1 sensor is designed from scratch (St-Pierre et al., 2014). The researchers inserted a circula ...
... thereby creating MacQ-mOrange2 and MacQ-mCitrine. This strategy combines the fast kinetic properties of the rhodopsin with the brightness conferred by the fluorescent proteins. On the other hand, Lin’s ASAP1 sensor is designed from scratch (St-Pierre et al., 2014). The researchers inserted a circula ...
File
... When bacteria take up a plasmid, we say they have been transformed Bacteria are encouraged to take up foreign DNA by: 1.heat-shock the bacteria at 42 C. followed by placing them on ice. 2. Place them in an electric field “electroporation” Then selection through selectable markers on the plasmid. ...
... When bacteria take up a plasmid, we say they have been transformed Bacteria are encouraged to take up foreign DNA by: 1.heat-shock the bacteria at 42 C. followed by placing them on ice. 2. Place them in an electric field “electroporation” Then selection through selectable markers on the plasmid. ...
Document
... The nucleotide sequence in DNA determines the amino acid sequence in proteins. A single change in that DNA sequence can affect a single amino acid, and may affect the structure and function of that protein. Because all biological processes are catalyzed by either RNA or protienaceous enzymes, and be ...
... The nucleotide sequence in DNA determines the amino acid sequence in proteins. A single change in that DNA sequence can affect a single amino acid, and may affect the structure and function of that protein. Because all biological processes are catalyzed by either RNA or protienaceous enzymes, and be ...
An archaebacterial homolog of pelota, a meiotic cell division protein
... are monophyletic [2,3], there is some evidence that they may be paraphyletic [4], with the crenarchaeotes as the sister-group of eukaryotes. In either case, archaebacteria and eukaryotes are thought to share a common ancestor more recent than that shared with eubacteria [2,4]. The specific common an ...
... are monophyletic [2,3], there is some evidence that they may be paraphyletic [4], with the crenarchaeotes as the sister-group of eukaryotes. In either case, archaebacteria and eukaryotes are thought to share a common ancestor more recent than that shared with eubacteria [2,4]. The specific common an ...
Isolation of a Complementary DNA Clone for the Human
... used to isolate the clone. Despite this error and the complexity of the oligonucleotide mixture, a weak hybridization signal was obtained in the initial screening of the cDNA library. The differences between the published and derived amino acid sequence at positions 6 and 10 could be the result of e ...
... used to isolate the clone. Despite this error and the complexity of the oligonucleotide mixture, a weak hybridization signal was obtained in the initial screening of the cDNA library. The differences between the published and derived amino acid sequence at positions 6 and 10 could be the result of e ...
Document
... The Hirschberg algorithm (Hirschberg, 1975) reduces the space requirements of a standard alignment algorithm from O(n2) to O(n) while leaving the time complexity O(n2), via a recursive procedure in which a decoding pass is made over the two halves of the matrix to determine the crossing point of the ...
... The Hirschberg algorithm (Hirschberg, 1975) reduces the space requirements of a standard alignment algorithm from O(n2) to O(n) while leaving the time complexity O(n2), via a recursive procedure in which a decoding pass is made over the two halves of the matrix to determine the crossing point of the ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.