13059_2010_2366_MOESM1_ESM
... molecular chaperones, alcohol dehydrogenases, thioredoxins, elicitin-like proteins, and a calcium binding protein. Genes coding for trehalose-phosphatase were also up-regulated (up to 10 fold) during heat stress, but not under the other abiotic conditions tested. In yeast and other organisms, treha ...
... molecular chaperones, alcohol dehydrogenases, thioredoxins, elicitin-like proteins, and a calcium binding protein. Genes coding for trehalose-phosphatase were also up-regulated (up to 10 fold) during heat stress, but not under the other abiotic conditions tested. In yeast and other organisms, treha ...
Last update: 06/22/2015
... homologs if they come from the same ancestral gene. Mutations in genes with an important biological function have a higher probability of being harmful to the organism and are less likely to become fixed in a population. Such sequences are said to be under negative selection, which causes them to be ...
... homologs if they come from the same ancestral gene. Mutations in genes with an important biological function have a higher probability of being harmful to the organism and are less likely to become fixed in a population. Such sequences are said to be under negative selection, which causes them to be ...
Localized hypermutation and associated gene losses in legume
... Point mutations result from errors made during DNA replication or repair, so they are usually expected to be homogeneous across all regions of a genome. However, we have found a region of chloroplast DNA in plants related to sweetpea (Lathyrus) whose local point mutation rate is at least 20 times hi ...
... Point mutations result from errors made during DNA replication or repair, so they are usually expected to be homogeneous across all regions of a genome. However, we have found a region of chloroplast DNA in plants related to sweetpea (Lathyrus) whose local point mutation rate is at least 20 times hi ...
Last update: 06/22/2015 Page 1 of 7 Introduction to BLAST using
... homologs if they come from the same ancestral gene. Mutations in genes with an important biological function have a higher probability of being harmful to the organism and are less likely to become fixed in a population. Such sequences are said to be under negative selection, which causes them to be ...
... homologs if they come from the same ancestral gene. Mutations in genes with an important biological function have a higher probability of being harmful to the organism and are less likely to become fixed in a population. Such sequences are said to be under negative selection, which causes them to be ...
A systematic search for DNA methyltransferase polymorphisms
... are de novo DNMTs which are responsible for establishing DNA methylation patterns during gametogenesis and early embryogenesis (15). The DNMT1 enzyme acts primarily as a maintenance DNMT ensuring the faithful replication of DNA methylation patterns at each cell division (16,17). In addition to these ...
... are de novo DNMTs which are responsible for establishing DNA methylation patterns during gametogenesis and early embryogenesis (15). The DNMT1 enzyme acts primarily as a maintenance DNMT ensuring the faithful replication of DNA methylation patterns at each cell division (16,17). In addition to these ...
Supplementary METHODS
... hour at 37ºC. Samples were irradiated with UVA light (365 nm, 1.8 J/cm2) to induce psoralen ICLs at the target site in the plasmid DNA. A 188 bp plasmid fragment surrounding the site-specific ICL was released by digestion with EcoRI and SacI enzymes, radiolabeled at the 5’ ends using T4 polynucleoti ...
... hour at 37ºC. Samples were irradiated with UVA light (365 nm, 1.8 J/cm2) to induce psoralen ICLs at the target site in the plasmid DNA. A 188 bp plasmid fragment surrounding the site-specific ICL was released by digestion with EcoRI and SacI enzymes, radiolabeled at the 5’ ends using T4 polynucleoti ...
Protein Synthesis 06-07
... Codon – a sequence of 3 bases on mRNA that specifies a specific amino acid that will be added to the polypeptide chain ...
... Codon – a sequence of 3 bases on mRNA that specifies a specific amino acid that will be added to the polypeptide chain ...
emboj201294-sup
... (A) Schematic representation of the generation of the Cdk7lox and Cdk7mut alleles from the Cdk7loxfrt allele present in ES clone D032B11 (German Gene Trap Consortium). (Top) Representation of the Cdk7loxfrt null allele generated by the insertion of the rFlipROSAbgeo gene-trap cassette within intron ...
... (A) Schematic representation of the generation of the Cdk7lox and Cdk7mut alleles from the Cdk7loxfrt allele present in ES clone D032B11 (German Gene Trap Consortium). (Top) Representation of the Cdk7loxfrt null allele generated by the insertion of the rFlipROSAbgeo gene-trap cassette within intron ...
Engineering Nitrogen Use Efficient Crop Plants
... involved in N metabolism, but may not play a direct role in NUE. Transcription Factors and Other Regulatory Proteins Dof1, a plant-specific transcription factor, is involved in the activation of non-photosynthetic, C4-related PEPc, as well as other organic acid metabolism proteins, and is up-regulat ...
... involved in N metabolism, but may not play a direct role in NUE. Transcription Factors and Other Regulatory Proteins Dof1, a plant-specific transcription factor, is involved in the activation of non-photosynthetic, C4-related PEPc, as well as other organic acid metabolism proteins, and is up-regulat ...
Wool Tech. and Sheep Breed. 50
... gel electrophoresis technology is probably too expensive. However, discovering which specific proteins are expressed in superior animals enables the use of high-throughput, immunochemistry laboratory or in-field assays to detect those protein markers. Alternatively, the process of gene detection and ...
... gel electrophoresis technology is probably too expensive. However, discovering which specific proteins are expressed in superior animals enables the use of high-throughput, immunochemistry laboratory or in-field assays to detect those protein markers. Alternatively, the process of gene detection and ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008.
... Therefore, chitin degradation and chitinases are of biotechnological importance. Chitinases degrade chitin into mainly mono and dimeric units of GlcNac. These enzymes are ubiquitous and are reported from bacteria, yeasts, higher fungi, plants and animals. In plants and animals, their role is mainly ...
... Therefore, chitin degradation and chitinases are of biotechnological importance. Chitinases degrade chitin into mainly mono and dimeric units of GlcNac. These enzymes are ubiquitous and are reported from bacteria, yeasts, higher fungi, plants and animals. In plants and animals, their role is mainly ...
Chapter 15
... • Francis Crick and Sydney Brenner determined how the order of nucleotides in DNA encoded amino acid order • Codon – block of 3 DNA nucleotides corresponding to an amino acid • Introduced single nulcleotide insertions or deletions and looked for mutations ...
... • Francis Crick and Sydney Brenner determined how the order of nucleotides in DNA encoded amino acid order • Codon – block of 3 DNA nucleotides corresponding to an amino acid • Introduced single nulcleotide insertions or deletions and looked for mutations ...
uric acid stimulates vascular smooth muscle cell proliferation by
... Literatures indicate that uric acid stimulates vascular smooth muscle cell (VSMC) proliferation in vitro. However, the related intracellular mechanisms of uric acids in VSMCs remain to be determined. Cultured rat aortic SMCs were stimulated with uric acid, [3H]thymidine incorporation and the endothe ...
... Literatures indicate that uric acid stimulates vascular smooth muscle cell (VSMC) proliferation in vitro. However, the related intracellular mechanisms of uric acids in VSMCs remain to be determined. Cultured rat aortic SMCs were stimulated with uric acid, [3H]thymidine incorporation and the endothe ...
Bolsum and PAM Matrix
... matches but there is now a need to include the bias associated with possible substitutions. • However, similarity does not necessarily imply common ancestor or visa versa Zvelebil and Baum (2008 p. 74) suggest this can occur in convergent evolution/divergent evolution. • So the results need to be co ...
... matches but there is now a need to include the bias associated with possible substitutions. • However, similarity does not necessarily imply common ancestor or visa versa Zvelebil and Baum (2008 p. 74) suggest this can occur in convergent evolution/divergent evolution. • So the results need to be co ...
NCEA Level 2 Biology (91159) 2013
... grow as well / big as those with high nutrients / limit potential genotype expression Not all environmental factors are mutagens, eg wind is not a mutagen because it doesn’t change the DNA sequence but does change phenotype. Genotype is not the only factor influencing gene expression however, as the ...
... grow as well / big as those with high nutrients / limit potential genotype expression Not all environmental factors are mutagens, eg wind is not a mutagen because it doesn’t change the DNA sequence but does change phenotype. Genotype is not the only factor influencing gene expression however, as the ...
8 DNA GENETIC TESTING - Centre for Genetics Education
... mutations that have been detected to date, at different places along the length of the gene involved in cystic fibrosis (CF) (see Genetics Fact Sheet 33). It is also likely that there are other mutations that have not yet been identified Laboratories often test for only some of the more commonly kno ...
... mutations that have been detected to date, at different places along the length of the gene involved in cystic fibrosis (CF) (see Genetics Fact Sheet 33). It is also likely that there are other mutations that have not yet been identified Laboratories often test for only some of the more commonly kno ...
Molecular evolution of the major chemosensory gene families in
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
Amino acid sequence homology in gag region of reverse
... The NBPcys amino acid sequences of reverse transcribing elements are shown in Fig. 2 together with adjacent amino acids. Some general features of a few retroviral NBPcys sequences have been noted previously [7] and the comparison is extended here to other elements. The arrangement of 3 cysteine resi ...
... The NBPcys amino acid sequences of reverse transcribing elements are shown in Fig. 2 together with adjacent amino acids. Some general features of a few retroviral NBPcys sequences have been noted previously [7] and the comparison is extended here to other elements. The arrangement of 3 cysteine resi ...
Phylogenetic, amino acid content and indel analyses
... sequences encoding conserved amino acid sequences in the rpoB genes of Mycobacterium tuberculosis (EMBL accession no. U12205), Mycobacterium leprae (Z14314), Mycobacterium smegmatis (U24494), Bacillus subtilis (L24376) and Staphylococcus aureus (X64172) and the rplL genes of Escherichia coli (V00339 ...
... sequences encoding conserved amino acid sequences in the rpoB genes of Mycobacterium tuberculosis (EMBL accession no. U12205), Mycobacterium leprae (Z14314), Mycobacterium smegmatis (U24494), Bacillus subtilis (L24376) and Staphylococcus aureus (X64172) and the rplL genes of Escherichia coli (V00339 ...
Worksheet 2
... The lowest position no. of these four numbers is: __________________ The highest position no. of these four numbers is: __________________ These two numbers stretch a region of how many nucleotides? __________________ Record the gi-number for this entry: _______________________________________ What ...
... The lowest position no. of these four numbers is: __________________ The highest position no. of these four numbers is: __________________ These two numbers stretch a region of how many nucleotides? __________________ Record the gi-number for this entry: _______________________________________ What ...
Lecture 6: Sequence Alignment – Local Alignment
... • This step uses a Smith-Waterman algorithm to create an optimised score (opt) for local alignment of query sequence to a each database sequence. • It takes a band of 32 letters centered on the init1 segment for calculating the optimal local alignment. • After all sequences in the database are searc ...
... • This step uses a Smith-Waterman algorithm to create an optimised score (opt) for local alignment of query sequence to a each database sequence. • It takes a band of 32 letters centered on the init1 segment for calculating the optimal local alignment. • After all sequences in the database are searc ...
GRASP-DNA: A Web Application to Screen Prokaryotic Genomes for
... Abstract The ability to control multiple genes at the transcriptional level often relies on the existence of short stretches of well-defined DNA sequences, to which regulatory proteins and transcription factors bind. In this article we present a freely accessible webbased application (GRASP-DNA), th ...
... Abstract The ability to control multiple genes at the transcriptional level often relies on the existence of short stretches of well-defined DNA sequences, to which regulatory proteins and transcription factors bind. In this article we present a freely accessible webbased application (GRASP-DNA), th ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.