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... Every 3 bases (triplet) on mRNA (codon) specifies an amino acid into a growing polypeptide chain (chain of protein) ○ 61 codons- code for amino acids ○ 3 codons- code to stop protein synthesis ○ 1 codon- codes to start protein synthesis (AUG- ...
CHNOPS Lab
CHNOPS Lab

... Genes are the units that determine inherited characteristics, such as hair color and blood type. Genes are lengths of DNA molecules that determine the structure of polypeptides (the building blocks of proteins) that our cells make. The sequence of nucleotides in DNA determines the sequence of amino ...
Topic 6 – Making Recombinant DNA Recombinant DNA – fragment
Topic 6 – Making Recombinant DNA Recombinant DNA – fragment

... Polymerase chain reaction (PCR) ‐ a technique for amplifying a DNA sequence by  repeated cycles of strand separation and replication o Allows the production of billions of pieces of DNA from small amounts of DNA ...
Chapter 5 Gases
Chapter 5 Gases

... • A typical cell in your body uses only about 10 percent of its genes at one time – Some genes affect structural features and metabolic pathways and are expressed in many cell types – Others genes are expressed only by certain subsets of cells (e.g., globin in RBCs) – Control over gene expression al ...
21 356 Molecular Biology Spring 2017
21 356 Molecular Biology Spring 2017

... start an exam after 15-minutes from the start time of the exam! No exceptions. There are NO make-up exams in this course; if you miss an exam then it will be counted as your dropped grade (Note: Everyone must take the final exam). ...
Trends in Biotechnology
Trends in Biotechnology

... plaques represents a library. g) Can calculate how many clones are needed to represent a genome. ...
Learning Objectives
Learning Objectives

... codons on mRNA and the linear sequence of amino acids in a polypeptide. 9. Explain the early techniques used to identify what amino acids are specified by the triplets UUU, AAA, GGG, and CCC. 10. Explain why polypeptides begin with methionine when they are synthesized. 11. Explain what it means to s ...
Learning Objectives
Learning Objectives

... codons on mRNA and the linear sequence of amino acids in a polypeptide. 9. Explain the early techniques used to identify what amino acids are specified by the triplets UUU, AAA, GGG, and CCC. 10. Explain why polypeptides begin with methionine when they are synthesized. 11. Explain what it means to s ...
Chapter 14 Guided Reading
Chapter 14 Guided Reading

... Chapter 14: Gene Expression from Gene to Protein 1. What is a gene? ...
Sai_Presentation
Sai_Presentation

... underexpressed • Gene characteristics ...
RNA & Protein Synthesis
RNA & Protein Synthesis

... RNA carries copies of genes – acts as “messengers” ◦ Messenger RNA or mRNA ...
THE lac OPERON
THE lac OPERON

... lactose are present This explains how the lac operon is transcribed only when lactose is present.  BUT….. this does not explain why the operon is not transcribed when both glucose and lactose are present. ...
Gene Section TOP1 (topoisomerase (DNA) 1) Atlas of Genetics and Cytogenetics
Gene Section TOP1 (topoisomerase (DNA) 1) Atlas of Genetics and Cytogenetics

... Location: 20q12-q13.1 Location (base pair): 39090K-39190K chromosome 20 Local order: centromer to telomer. ...
Introduction to Biotechnology
Introduction to Biotechnology

... It can be used to identify genetic disorders, either from karyotyping or from genetic analysis (mapping of genome) ...
Yeast microbes are probably one of the earliest
Yeast microbes are probably one of the earliest

... that allows the yeast to bring sugar into the cell (called S1). Once in the cell, the bonds in sugar are broken in a process called glycolysis. If the cells are living in a low oxygen environment (anaerobic), and the second gene of interest is functioning (called F1) then fermentation occurs and the ...
Document
Document

... glycine ...
The Importance of DNA and RNA - Emmanuel Biology 12
The Importance of DNA and RNA - Emmanuel Biology 12

... The unwound DNA exposes two parental strands of DNA which are antiparallel. This means they are orientated in different directions and must be replicated by different mechanisms. The leading strand elongates towards the replication fork (in the direction of unwinding) by the simple addition of nucle ...
RNA & Transcription
RNA & Transcription

... 5) RNA IS EDITED: sections removed are called Introns while the parts that stay are called exons. The parts of the primary transcript called introns are cut out. Introns appear to match noncoding regions of DNA. In order for this to happen, “Snurps” (snRNA & Protein complexes) bind to form spliceoso ...
Genetic Variation: Horizontal Gene Transfer
Genetic Variation: Horizontal Gene Transfer

... • One of the unexpected findings of the first comparisons of complete bacterial genomes has been the near lack of gene order conservation, beyond the level of operons, even between relatively close species such as Escherichia coli and Haemophilus influenzae (Koonin et al. 1996; Tatusov et al. 1996) ...
Chapter 1
Chapter 1

... Fig. 1.3 Diagram of the experiment demonstrating that DNA is the active material in bacterial transformation © 2006 Jones & Bartlett Publishers ...
App1PCR - FSU Biology
App1PCR - FSU Biology

... to yield microgram quantities for subsequent biochemical analysis. In order to perform PCR, the DNA sequence flanking both sides of the target must be known. (Note that PCR cannot be used as a substitute for cloning unknown genes!) This information is used to synthesize short oligonucleotides (singl ...
Unit 8.3: Biotechnology
Unit 8.3: Biotechnology

... strands. This yields two single strands of DNA. 1. Annealing involves cooling the single strands of DNA and mixing them with short DNA segments called primers. Primers have base sequences that are complementary to segments of the single DNA strands. As a result, bonds form between the DNA strands an ...
Chapter 12 DNA and RNA - Northwestern High School
Chapter 12 DNA and RNA - Northwestern High School

... • At any point DNA can begin replication (replication fork) • Helicases separate the DNA strands. • Enzyme called DNA polymerase start the replication • Semi-conservative – not all of it is new. • Replicates in both directions until done, read in only one direction. ...
PDF
PDF

... In molecular terms, epigenetics is concerned with the study of chromatin organisation in the eukaryotic nucleus, with respect to the regulation of gene expression during the development of plants, fungi, insects and animals through embryogenesis to adulthood, and in disease states. A major intellect ...
PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... Screen progeny DNA for KO genotype (X-/X-). ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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