Signals and mechanisms for protein retention in the endoplasmic
... the human erd2 gene is not. It was first thought that the ER-resident ERD receptors would bind the K/HDEL retention signal to divert reticuloplasmins from the bulk flow of secreted proteins. There is now some evidence, at least in mammals and yeast, that these receptors do not stricto sensu retain r ...
... the human erd2 gene is not. It was first thought that the ER-resident ERD receptors would bind the K/HDEL retention signal to divert reticuloplasmins from the bulk flow of secreted proteins. There is now some evidence, at least in mammals and yeast, that these receptors do not stricto sensu retain r ...
Supplementary Figure 1
... HSA-binding proteins can interfere with the fluorescence linearity from FITC-HSA. To assess the fluorescence linearity by input FITC-HSA, the band intensities of FITC-HSA were calculated using densitometry software (Phoretix 2D Expression program, Nonlinear Dynamics, Durham, NC). The background was ...
... HSA-binding proteins can interfere with the fluorescence linearity from FITC-HSA. To assess the fluorescence linearity by input FITC-HSA, the band intensities of FITC-HSA were calculated using densitometry software (Phoretix 2D Expression program, Nonlinear Dynamics, Durham, NC). The background was ...
05. Amino acids, Protein
... Enzymes, the proteins that direct and accelerate thousands of biochemical reactions Structure. Some proteins function as structural materials that provide protection and support. Movement. Proteins are involved in all types of cell movement. For example, actin, tubulin, and а variety of other prot ...
... Enzymes, the proteins that direct and accelerate thousands of biochemical reactions Structure. Some proteins function as structural materials that provide protection and support. Movement. Proteins are involved in all types of cell movement. For example, actin, tubulin, and а variety of other prot ...
Polymer - Deans Community High School
... Condensation Polymers Polymers are very large molecules made by joining small molecules, monomers, in long chains or networks. Condensation polymers are made from monomers with two functional groups per molecule. Starch is made by polymerisation of about 300 glucose molecules ...
... Condensation Polymers Polymers are very large molecules made by joining small molecules, monomers, in long chains or networks. Condensation polymers are made from monomers with two functional groups per molecule. Starch is made by polymerisation of about 300 glucose molecules ...
Molecular and Cellular Biology, December 2001, p
... a nonremovable N-terminal ubiquitin (Ub) moiety had a short half-life (19). The protein degradation pathway involved was called UFD, for Ub fusion degradation. A genetic approach was used to dissect this pathway, and five genes termed UFD1 to UFD5 were discovered to be involved in the degradation of ...
... a nonremovable N-terminal ubiquitin (Ub) moiety had a short half-life (19). The protein degradation pathway involved was called UFD, for Ub fusion degradation. A genetic approach was used to dissect this pathway, and five genes termed UFD1 to UFD5 were discovered to be involved in the degradation of ...
Lecture outlines: RNA to proteins
... range in size from 73-93 nucleotides long, with substantial duplex regions and elaborate structure (Fig. 7.27). Contain anticodon loops with specific sequence that will base-pair with the codons on mRNA. 3’ end is the site where the appropriate amino acid will be attached. ...
... range in size from 73-93 nucleotides long, with substantial duplex regions and elaborate structure (Fig. 7.27). Contain anticodon loops with specific sequence that will base-pair with the codons on mRNA. 3’ end is the site where the appropriate amino acid will be attached. ...
Document
... identified by photometric plate reader (SpectraMax 250) and pooled for further analysis. Quantify F/P ratio is done by the 2nd derivativeanalysis of measured spectra: (494nm fluorescien)/(301nm protein). ...
... identified by photometric plate reader (SpectraMax 250) and pooled for further analysis. Quantify F/P ratio is done by the 2nd derivativeanalysis of measured spectra: (494nm fluorescien)/(301nm protein). ...
2012-ISB-symposium
... exploration of mass spectrometry detected peptides and their structural locations. Studying patterns of peptide location across a protein can be used for many purposes: exploring PTM (post translational modification) locations with respect to putative protein active sites and protein-protein interac ...
... exploration of mass spectrometry detected peptides and their structural locations. Studying patterns of peptide location across a protein can be used for many purposes: exploring PTM (post translational modification) locations with respect to putative protein active sites and protein-protein interac ...
H - Free
... Between charged groups on the biological receptor and oppositely charged groups on the transducer surface. These are mainly used for immobilisation of DNA. 3.2 Physical adsorption to the surface Many materials (e.g. glass, gold, silica gel) adsorb proteins on their surfaces. No reagents are required ...
... Between charged groups on the biological receptor and oppositely charged groups on the transducer surface. These are mainly used for immobilisation of DNA. 3.2 Physical adsorption to the surface Many materials (e.g. glass, gold, silica gel) adsorb proteins on their surfaces. No reagents are required ...
Ex vivo analysis of splicing assays
... how to improve our understanding of new disease-causing mutations, that do not affect the coding potential of a gene, but that affect pre-mRNA splicing. In order to analyse precise mechanism of alternative splicing most laboratories use socalled mini-gene reporters. These reporters can easily be tra ...
... how to improve our understanding of new disease-causing mutations, that do not affect the coding potential of a gene, but that affect pre-mRNA splicing. In order to analyse precise mechanism of alternative splicing most laboratories use socalled mini-gene reporters. These reporters can easily be tra ...
Gene Section USP15 (ubiquitin specific peptidase 15) Atlas of Genetics and Cytogenetics
... may underpin recent reports that USP15 levels can influence the taxol sensitivity of cancer cells (Xu et al., 2009; Xie et al., 2010). VCP/p97 is a large AAA+-type ATPase that acts as a chaperone in many cellular processes. Its basic function is to segregate ubiquitinated proteins from macromolecula ...
... may underpin recent reports that USP15 levels can influence the taxol sensitivity of cancer cells (Xu et al., 2009; Xie et al., 2010). VCP/p97 is a large AAA+-type ATPase that acts as a chaperone in many cellular processes. Its basic function is to segregate ubiquitinated proteins from macromolecula ...
Nucleolar translocalization of GRA10 of Toxoplasma gondii
... nents and subcellular organelles across the toxoplasmal PVM. In the PV and PVM, many dense granular proteins are found to be secreted to decorate the TVN of PV and PVM in addition to rhoptry proteins. These GRA proteins are suggested to be the key proteins in the maintenance of relationship between ...
... nents and subcellular organelles across the toxoplasmal PVM. In the PV and PVM, many dense granular proteins are found to be secreted to decorate the TVN of PV and PVM in addition to rhoptry proteins. These GRA proteins are suggested to be the key proteins in the maintenance of relationship between ...
Transcription. (Ms. Shivani Bhagwat)
... by RNA polymerase II. Transcription copies the DNA code of a gene and converts it to high mol mass nuclear RNA (hnRNA), which is precessed to mRNA. The mRNA will be used at the ribosome to make polypeptides (proteins). RNA polymerase II is a multisubunit enzyme-complex.The yeast enzyme has 12 subuni ...
... by RNA polymerase II. Transcription copies the DNA code of a gene and converts it to high mol mass nuclear RNA (hnRNA), which is precessed to mRNA. The mRNA will be used at the ribosome to make polypeptides (proteins). RNA polymerase II is a multisubunit enzyme-complex.The yeast enzyme has 12 subuni ...
Proteins, the Essence of Life
... point the mRNA is an immature mRNA molecule and must be processed. In eukaryotic genes, there are nucleotides that are not used or expressed. One might view these DNA nucleotides as “junk DNA”. They are called introns because they are intervening sequences. RNA polymerase can not tell the difference ...
... point the mRNA is an immature mRNA molecule and must be processed. In eukaryotic genes, there are nucleotides that are not used or expressed. One might view these DNA nucleotides as “junk DNA”. They are called introns because they are intervening sequences. RNA polymerase can not tell the difference ...
Membrane Proteins: Capturing the signal | eLife
... existence of this signal peptide (Blobel and Dobberstein, 1975), and the signal recognition particle (SRP), which actively targets the proteins to the endoplasmic reticulum, was also eventually identified (Walter and Blobel, 1981a). The SRP is thought to bind to and protect the signal peptide (which ...
... existence of this signal peptide (Blobel and Dobberstein, 1975), and the signal recognition particle (SRP), which actively targets the proteins to the endoplasmic reticulum, was also eventually identified (Walter and Blobel, 1981a). The SRP is thought to bind to and protect the signal peptide (which ...
Defining the inner membrane proteome of E coli
... • Incorporation of experimental topology information improves the topology models • These papers have been a nice cooperation between experimentalists and bioinformaticians, where both have benefited from each others results ...
... • Incorporation of experimental topology information improves the topology models • These papers have been a nice cooperation between experimentalists and bioinformaticians, where both have benefited from each others results ...
structural
... 20 AA’s found in proteins, with different chemical properties. Of note is cysteine, which can form covalent bonds to other cysteines through a disulfide linkage. ...
... 20 AA’s found in proteins, with different chemical properties. Of note is cysteine, which can form covalent bonds to other cysteines through a disulfide linkage. ...
Chapter 3 Amino Acids, Peptides, Proteins
... Need a few mg of protein Some acids destroyed in base, others in acids, so usually need both acid and base hydrolysis can double check results against MW to be sure you have everything Note - this only tells you overall % composition, NOT sequence. For that you need to dig harder C. Sequence Determi ...
... Need a few mg of protein Some acids destroyed in base, others in acids, so usually need both acid and base hydrolysis can double check results against MW to be sure you have everything Note - this only tells you overall % composition, NOT sequence. For that you need to dig harder C. Sequence Determi ...
L1 Protein composition-amino acids - e
... 20 common amino acids build all proteins in living cells. All of them are α-amino acids. All of them have a carboxyl and an amino group bonded to α-carbon atom. α -amino acids differ from each other by their side chains, or R-groups. R-groups are different in structure, size, and electric ...
... 20 common amino acids build all proteins in living cells. All of them are α-amino acids. All of them have a carboxyl and an amino group bonded to α-carbon atom. α -amino acids differ from each other by their side chains, or R-groups. R-groups are different in structure, size, and electric ...
Thesis - u
... presenting the characteristics of non-cell autonomous proteins (NCAP). The PP2 proteins have also been shown to be RNA-binding proteins, suggesting that in addition to the shuttling of proteins from companion cells to the sieve elements, they can also be part of large ribonucleoprotein phloem comple ...
... presenting the characteristics of non-cell autonomous proteins (NCAP). The PP2 proteins have also been shown to be RNA-binding proteins, suggesting that in addition to the shuttling of proteins from companion cells to the sieve elements, they can also be part of large ribonucleoprotein phloem comple ...
PINdb: a database of nuclear protein complexes from human and
... co-activators, co-repressors and chromatin remodelers as well as sequence-specific transcriptional factors. As is the norm for proteins extracted from the cell, those involved in the transcription process often co-purify as large macromolecular complexes, and it is not unusual for some complexes to ...
... co-activators, co-repressors and chromatin remodelers as well as sequence-specific transcriptional factors. As is the norm for proteins extracted from the cell, those involved in the transcription process often co-purify as large macromolecular complexes, and it is not unusual for some complexes to ...
Proteins with
... 2) Sequences above a certain threshold (< specified E-value) are included. Assumed to be related proteins. This group of sequences is used to define a “profile” that contains the sequence “essence” of the protein family. 3) Now with the important sequence positions highlighted, can look for more dis ...
... 2) Sequences above a certain threshold (< specified E-value) are included. Assumed to be related proteins. This group of sequences is used to define a “profile” that contains the sequence “essence” of the protein family. 3) Now with the important sequence positions highlighted, can look for more dis ...
Identification of cAMP-dependent phosphorylated proteins involved
... of Perkinsus marinus, and actin of Karlodinium micrum, respectively (Table 1).The hypothetical protein (p21) failed to be identified, because no proteins highly homologous to the T. thermophila hypothetical protein were found. RISP has been suggested to have functions other than a core polypeptide o ...
... of Perkinsus marinus, and actin of Karlodinium micrum, respectively (Table 1).The hypothetical protein (p21) failed to be identified, because no proteins highly homologous to the T. thermophila hypothetical protein were found. RISP has been suggested to have functions other than a core polypeptide o ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.