Journal of Bacteriology
... The nitrogenase of the free-living, microaerobic, N2-fixing bacterium AzospiriUum amazonense (strain Y1) was purified by chromatography on DEAE-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. The specific nitrogenase activities were 2,400 nmol of C21[4 formed ...
... The nitrogenase of the free-living, microaerobic, N2-fixing bacterium AzospiriUum amazonense (strain Y1) was purified by chromatography on DEAE-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. The specific nitrogenase activities were 2,400 nmol of C21[4 formed ...
Huntingtin: Its Role in Gene Expression
... transcripts undergo to remove unnecessary strings of sequence. During the process, RBPs recruit proteins to remove the extraneous sections of RNA (introns) and splice together the remaining sections (exons). The RNA can be spliced in several different ways to produce multiple transcripts from one ge ...
... transcripts undergo to remove unnecessary strings of sequence. During the process, RBPs recruit proteins to remove the extraneous sections of RNA (introns) and splice together the remaining sections (exons). The RNA can be spliced in several different ways to produce multiple transcripts from one ge ...
Articulins and epiplasmins - Journal of Cell Science
... For affinity selection, a rabbit antiserum (serum 018) raised against SDS-denatured epiplasm proteins of P. dubius was used (Peck et al., 1991). For the affinity selection of articulin VPV-repeat motif-specific antibodies the central VPV-repeat motif domain of P. dubius articulin p60 was expressed a ...
... For affinity selection, a rabbit antiserum (serum 018) raised against SDS-denatured epiplasm proteins of P. dubius was used (Peck et al., 1991). For the affinity selection of articulin VPV-repeat motif-specific antibodies the central VPV-repeat motif domain of P. dubius articulin p60 was expressed a ...
Theoretical studies of Membrane Proteins
... and 3D structures. Prediction of the membrane spanning regions by hydrophobicity analysis is a key technique used in several of the studies. A novel method for this is also presented and compared to other methods. The primary questions addressed in the thesis are: What properties are common to all m ...
... and 3D structures. Prediction of the membrane spanning regions by hydrophobicity analysis is a key technique used in several of the studies. A novel method for this is also presented and compared to other methods. The primary questions addressed in the thesis are: What properties are common to all m ...
Metabolic adaptation of Mycobacterium avium subsp
... GE Healthcare) using the differential in-gel analysis module. Quantification was applied for filter-confirmed spots with slope .1.4, area ,420 and volume ,130 000. Proteins were considered as differentially expressed if an at least 1.5-fold expression difference was determined between BDC and CDC. N ...
... GE Healthcare) using the differential in-gel analysis module. Quantification was applied for filter-confirmed spots with slope .1.4, area ,420 and volume ,130 000. Proteins were considered as differentially expressed if an at least 1.5-fold expression difference was determined between BDC and CDC. N ...
Article, Discoveries Variation in mutational robustness between
... (Schenk et al. 2012). The fitness increases for the mutations in araC and araE were relatively small (s ¼ 0.0061–0.019) and would not have been detected using less sensitive fitness assays, but as most mutations are expected from theory to have small effects (Fisher 1930) this again highlights the i ...
... (Schenk et al. 2012). The fitness increases for the mutations in araC and araE were relatively small (s ¼ 0.0061–0.019) and would not have been detected using less sensitive fitness assays, but as most mutations are expected from theory to have small effects (Fisher 1930) this again highlights the i ...
The significance of biochemical and molecular sample integrity in
... state and the rapidity of death, may play major roles in determining the postmortem condition of the sample [6–9]. It would therefore be of importance to determine the condition and quality of postmortem tissue, for example, through the quantification of a biological marker in such sample. In a prev ...
... state and the rapidity of death, may play major roles in determining the postmortem condition of the sample [6–9]. It would therefore be of importance to determine the condition and quality of postmortem tissue, for example, through the quantification of a biological marker in such sample. In a prev ...
Peptide Formulation: Challenges and Strategies
... in the case of Asn, deamidation of Asn tends to proceed more quickly than for Gln, although both have been observed (1). For peptides, the high degree of flexibility of the peptide chain leads to maximal rates of deamidation, although it is important to note that the nature of the succeeding amino a ...
... in the case of Asn, deamidation of Asn tends to proceed more quickly than for Gln, although both have been observed (1). For peptides, the high degree of flexibility of the peptide chain leads to maximal rates of deamidation, although it is important to note that the nature of the succeeding amino a ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
... phosphoproteins involved in the heat shock response, cellular metabolism and protein synthesis. This work also identified changes in the phosphoproteome that were dependent upon the presence or absence of RdoA. Phosphoproteins that showed a significant change in phosphorylation were identified by ma ...
... phosphoproteins involved in the heat shock response, cellular metabolism and protein synthesis. This work also identified changes in the phosphoproteome that were dependent upon the presence or absence of RdoA. Phosphoproteins that showed a significant change in phosphorylation were identified by ma ...
Crossing borders to bind proteins—a new concept in protein
... molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein but only modest affinity is required. Conjugation to ...
... molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein but only modest affinity is required. Conjugation to ...
Doc S1.
... Mitochondrial RNAs (COX1, COX2) are used to normalise Q-PCR results and determine mitochondrial purification yield (η). This yield takes into consideration efficiency of different steps from biochemical purification to RT PCR. ...
... Mitochondrial RNAs (COX1, COX2) are used to normalise Q-PCR results and determine mitochondrial purification yield (η). This yield takes into consideration efficiency of different steps from biochemical purification to RT PCR. ...
Contribution of defined amino acid residues to the immunogenicity
... nated the pEHProSTC28-speci¢ed hybrid as the highest toxic chimera (Fig. 1). In contrast, the secreted fusion protein from pSTCDM1+pDSPH524 with glycine and leucine simultaneously substituted for Pro13 and Ala14 residues of STh revealed no toxin activity (Fig. 1). As assessed by immunoblotting analy ...
... nated the pEHProSTC28-speci¢ed hybrid as the highest toxic chimera (Fig. 1). In contrast, the secreted fusion protein from pSTCDM1+pDSPH524 with glycine and leucine simultaneously substituted for Pro13 and Ala14 residues of STh revealed no toxin activity (Fig. 1). As assessed by immunoblotting analy ...
proteins: three-dimensional structure
... polypeptides, each of which forms an a helix, twisting around each other to form a left-handed coil. The normal 5.4-Å repeat distance of each a helix in the pair is thereby tilted relative to the axis of this assembly, yielding the observed 5.1f Å spacing. The assembly is said to have a coiled coil ...
... polypeptides, each of which forms an a helix, twisting around each other to form a left-handed coil. The normal 5.4-Å repeat distance of each a helix in the pair is thereby tilted relative to the axis of this assembly, yielding the observed 5.1f Å spacing. The assembly is said to have a coiled coil ...
Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic
... amino acid meal, and then midgut epithelial cells were analyzed by electron microscopy and protein biochemistry. The size and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression ...
... amino acid meal, and then midgut epithelial cells were analyzed by electron microscopy and protein biochemistry. The size and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression ...
A Cyanobacterial Chlorophyll Synthase-HliD
... detected a specific signal for SecY for the FLAG-ChlG pulldown, as well as a signal for the Rpl1 ribosome subunit that is clearly stronger than in the control (Figure 1B). We also investigated the ChlG-Ycf39 interaction using an anti-Ycf39 antibody to immunoprecipitate Ycf39 from solubilized membrane ...
... detected a specific signal for SecY for the FLAG-ChlG pulldown, as well as a signal for the Rpl1 ribosome subunit that is clearly stronger than in the control (Figure 1B). We also investigated the ChlG-Ycf39 interaction using an anti-Ycf39 antibody to immunoprecipitate Ycf39 from solubilized membrane ...
Protein sorting at the ER–Golgi interface
... also known as coatomer, consists of seven subunits (α, β, β', γ, δ, ε, and ζ-COP), which are recruited as an intact complex to membranes (Hara-Kuge et al., 1994). Coatomer can be biochemically divided into two subcomplexes: a trimer composed of α, β', and ε, named the B-subcomplex; and a tetramer, c ...
... also known as coatomer, consists of seven subunits (α, β, β', γ, δ, ε, and ζ-COP), which are recruited as an intact complex to membranes (Hara-Kuge et al., 1994). Coatomer can be biochemically divided into two subcomplexes: a trimer composed of α, β', and ε, named the B-subcomplex; and a tetramer, c ...
7.3 Translation (HL ONLY)
... • Bound ribosomes synthesize proteins primarily for secretion or for use in lysosomes. • Translation can occur immediately after transcription in prokaryotes due to the absence of a nuclear membrane. ...
... • Bound ribosomes synthesize proteins primarily for secretion or for use in lysosomes. • Translation can occur immediately after transcription in prokaryotes due to the absence of a nuclear membrane. ...
vts_6580_8973.
... bound to GDP and an active one that is bound to GTP. The switching from one state to the other is regulated by upstream signals, which control the transition by favouring the exchange of GDP for GTP. In the active GTP-bound state, the Ras ...
... bound to GDP and an active one that is bound to GTP. The switching from one state to the other is regulated by upstream signals, which control the transition by favouring the exchange of GDP for GTP. In the active GTP-bound state, the Ras ...
PDF + SI - GenScript
... lthough in recent years there has been significant progress in our understanding of the molecular mechanisms of eukaryotic DNA replication, the identity of the primary activity that unravels duplex DNA at the growing fork has remained unclear. Based on a range of indirect data, there is widespread s ...
... lthough in recent years there has been significant progress in our understanding of the molecular mechanisms of eukaryotic DNA replication, the identity of the primary activity that unravels duplex DNA at the growing fork has remained unclear. Based on a range of indirect data, there is widespread s ...
The Other Lives of Ribosomal Proteins - PDXScholar
... are major components of the ribosome and play a significant role in the initiation, elongation, or termination phase of protein translation (Wang et al., 2014; Nissen et al., 2000). Therefore, this indicates that ribosomal proteins are essential for both ribosome formation and protein translation. H ...
... are major components of the ribosome and play a significant role in the initiation, elongation, or termination phase of protein translation (Wang et al., 2014; Nissen et al., 2000). Therefore, this indicates that ribosomal proteins are essential for both ribosome formation and protein translation. H ...
Document
... >50 amino acids), the protein exceeds that which can be handled on a peptide synthesizer (∼ so the protein has to be expressed in E. coli. Second and more importantly, the amide I frequency is downshifted into a spectral range were many amino acid side chains absorb as well, in particular those cont ...
... >50 amino acids), the protein exceeds that which can be handled on a peptide synthesizer (∼ so the protein has to be expressed in E. coli. Second and more importantly, the amide I frequency is downshifted into a spectral range were many amino acid side chains absorb as well, in particular those cont ...
Coarse-Grained Modeling of ProteinDynamics
... 2010; Best and Hummer 2008). Despite their simplicity, in many cases they correctly capture unfolding pathways, FEL and mechanical stability of proteins. For example, a complete description of mechanical unfolding pathways of single and multidomain Ubiquitin at the level of secondary structure was o ...
... 2010; Best and Hummer 2008). Despite their simplicity, in many cases they correctly capture unfolding pathways, FEL and mechanical stability of proteins. For example, a complete description of mechanical unfolding pathways of single and multidomain Ubiquitin at the level of secondary structure was o ...
Activity of ribosomes and tmRNA of Streptomyces aureofaciens
... producer Streptomyces aureofaciens under temperature shift up and down and possible role of transtranslation system. Tetracycline inhibits protein synthesis by interfering with the binding of aminoacyltRNA to the A-site of ribosome [14]. There are at least two functionally important binding sites fo ...
... producer Streptomyces aureofaciens under temperature shift up and down and possible role of transtranslation system. Tetracycline inhibits protein synthesis by interfering with the binding of aminoacyltRNA to the A-site of ribosome [14]. There are at least two functionally important binding sites fo ...
OLSON LAB PROTOCOL: Working with RNA
... NOTES from the Qiagen RNeasy Mini Handbook 09/2010 Handling and storing starting material RNA in animal and plant tissues is not protected after harvesting until the sample is treated with RNAlater RNA Stabilization Reagent (animal tissues only), flash-frozen, or disrupted and homogenized in the pre ...
... NOTES from the Qiagen RNeasy Mini Handbook 09/2010 Handling and storing starting material RNA in animal and plant tissues is not protected after harvesting until the sample is treated with RNAlater RNA Stabilization Reagent (animal tissues only), flash-frozen, or disrupted and homogenized in the pre ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.