RNA notes 2015 - OG
... Gene Sequencing – Determining the order of nucleotide bases within a gene DNA Fingerprinting – technique used in criminal investigations. DNA Fingerprinting takes the DNA out of a cell and separates it. This will allow investigators to distinguish body cells of different individuals (since they are ...
... Gene Sequencing – Determining the order of nucleotide bases within a gene DNA Fingerprinting – technique used in criminal investigations. DNA Fingerprinting takes the DNA out of a cell and separates it. This will allow investigators to distinguish body cells of different individuals (since they are ...
Lecture 1
... The leading strand is that strand of the DNA double helix that is oriented in a 5' to 3' manner. On the leading strand, a polymerase "reads" the DNA and adds nucleotides to it continuously. This polymerase is DNA polymerase III (DNA Pol III) in prokaryotes The lagging strand is that strand of the DN ...
... The leading strand is that strand of the DNA double helix that is oriented in a 5' to 3' manner. On the leading strand, a polymerase "reads" the DNA and adds nucleotides to it continuously. This polymerase is DNA polymerase III (DNA Pol III) in prokaryotes The lagging strand is that strand of the DN ...
Total RNA MinElute Cleanup - Yale Center for Genome Analysis
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
CHAPTER 17 FROM GENE TO PROTEIN
... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
3.4 C: Transcription Quiz PROCTOR VERSION
... The resulting RNA transcript is due to a single substitution in the DNA sequence. Distractor Rationale: This answer suggests the student may understand that a substitution in the DNA sequence will result in a change in the resulting RNA sequence, but does not understand that the given RNA transcript ...
... The resulting RNA transcript is due to a single substitution in the DNA sequence. Distractor Rationale: This answer suggests the student may understand that a substitution in the DNA sequence will result in a change in the resulting RNA sequence, but does not understand that the given RNA transcript ...
Document
... are utilized, with the aid of a ribosome, to form a polypeptide chain that, when finished, will be a protein. tRNA is fed into the ribosome and the anticodon is detached and added to the polypeptide chain. A ribosome is a complex of proteins and rRNA. ...
... are utilized, with the aid of a ribosome, to form a polypeptide chain that, when finished, will be a protein. tRNA is fed into the ribosome and the anticodon is detached and added to the polypeptide chain. A ribosome is a complex of proteins and rRNA. ...
The Origins of Life and Precambrian Evolution
... differences in the speed of self-replication or in chemical stability – In this case, the “genotype” is the chemical structure of the molecule, and the “phenotype” is the speed of selfreplication or stability of the molecule ...
... differences in the speed of self-replication or in chemical stability – In this case, the “genotype” is the chemical structure of the molecule, and the “phenotype” is the speed of selfreplication or stability of the molecule ...
This is an example of a slide
... Srb/mediator complex (conserved yeast – mammals) 24 subunits associated with RNA polymerase II involved in regulation of transcription Role of different subunits in yeast? CDK-Cyclin ...
... Srb/mediator complex (conserved yeast – mammals) 24 subunits associated with RNA polymerase II involved in regulation of transcription Role of different subunits in yeast? CDK-Cyclin ...
PASS MOCK EXAM
... A) Nonsense mutation; will. B) Missense mutation; will not. C) Nonsense mutation; will. D) Missense mutation; will not. E) Silent mutation; will not. ...
... A) Nonsense mutation; will. B) Missense mutation; will not. C) Nonsense mutation; will. D) Missense mutation; will not. E) Silent mutation; will not. ...
Nucleotide Functions Nucleic Acid Structure Nucleic Acid Sequence
... Histones are rich in the basic amino acids lysine and arginine, which have positive charges. These positively charged residues provide binding for the negatively charged ribose-phosphate chain of DNA. ...
... Histones are rich in the basic amino acids lysine and arginine, which have positive charges. These positively charged residues provide binding for the negatively charged ribose-phosphate chain of DNA. ...
Deamination of 5-methylcytosine yields thymine
... DNA but not RNA. 2. Provide a biochemical rationale for why AT-rich sequences are commonly found in zones of initiation of DNA replication and near sites of transcriptional termination. Only two hydrogen bonds needed for base-pairing between adenine and thymine versus three for guanine and cytosine. ...
... DNA but not RNA. 2. Provide a biochemical rationale for why AT-rich sequences are commonly found in zones of initiation of DNA replication and near sites of transcriptional termination. Only two hydrogen bonds needed for base-pairing between adenine and thymine versus three for guanine and cytosine. ...
E. Nucleotide sequences that define an intron. Mutations in
... C. General Transcription factors participate in recruiting RNA polymerase to the gene. Model for Pre-initiation Complex (PIC) Assembly on a TATA-containing Core Promoter. TFIID, TFIIA and TFIIB bind to the promoter in an early step. TFIID is composed of the TATA binding protein (TBP) and TBP-associ ...
... C. General Transcription factors participate in recruiting RNA polymerase to the gene. Model for Pre-initiation Complex (PIC) Assembly on a TATA-containing Core Promoter. TFIID, TFIIA and TFIIB bind to the promoter in an early step. TFIID is composed of the TATA binding protein (TBP) and TBP-associ ...
Understanding the regulation of surfactant gene expression EDITORIAL W. Jacot, J. Bousquet
... Among a number of biochemical and morphological criteria, alveolar type-II epithelial cells are defined by the synthesis of surfactant proteins. These molecules are implicated in numerous human diseases such as neonatal respiratory distress syndrome [1] and possibly acute respiratory distress syndro ...
... Among a number of biochemical and morphological criteria, alveolar type-II epithelial cells are defined by the synthesis of surfactant proteins. These molecules are implicated in numerous human diseases such as neonatal respiratory distress syndrome [1] and possibly acute respiratory distress syndro ...
Transcription Translation Notes
... What do we know about the genetic code (for a protein) There are four DNA bases: Adenine, Thymine, Cytosine, Guanine The DNA bases complimentary to each other: A-T & C-G Three nitrogenous bases code for one amino acid (triplet = codon, or a 3-base code) Gene is a section of DNA that codes for a spe ...
... What do we know about the genetic code (for a protein) There are four DNA bases: Adenine, Thymine, Cytosine, Guanine The DNA bases complimentary to each other: A-T & C-G Three nitrogenous bases code for one amino acid (triplet = codon, or a 3-base code) Gene is a section of DNA that codes for a spe ...
Analytical Questions
... 4. The primary structure of a protein is the linear order of amino acids in the polypeptide chain joined by covalent peptide bonds. The secondary structure refers to the formation of either -helices or pleated sheets by the peptide chain. -helices are stabilized by hydrogen bonding between the s ...
... 4. The primary structure of a protein is the linear order of amino acids in the polypeptide chain joined by covalent peptide bonds. The secondary structure refers to the formation of either -helices or pleated sheets by the peptide chain. -helices are stabilized by hydrogen bonding between the s ...
R N A & PROTEIN SYNTHESIS
... which takes place on ribosomes Amino Acids are transported by ribosomes & tRNA molecules, which have specific regions that bond to AA The loop attachment has a sequence of 3 nucleotides called anticodons. The tRNA anticodon is complementary & pairs with the mRNA codons. During translation or protein ...
... which takes place on ribosomes Amino Acids are transported by ribosomes & tRNA molecules, which have specific regions that bond to AA The loop attachment has a sequence of 3 nucleotides called anticodons. The tRNA anticodon is complementary & pairs with the mRNA codons. During translation or protein ...
et al
... Figure 3.11. Comparison of the transcriptomes of different types of human cell. The diagram shows human chromosome 11 aligned vertically. The bar charts indicate the expression levels in different cell types of the genes on this chromosome. The lengths of the blue bars are proportional to the exten ...
... Figure 3.11. Comparison of the transcriptomes of different types of human cell. The diagram shows human chromosome 11 aligned vertically. The bar charts indicate the expression levels in different cell types of the genes on this chromosome. The lengths of the blue bars are proportional to the exten ...
DNA, RNA, Protein Synthesis Notes
... 9-12.L.1.1. Students are able to relate cellular functions and processes to specialized structures within cells. • Role of enzymes • Storage and transfer of genetic information Examples: replication, transcription, and translation ...
... 9-12.L.1.1. Students are able to relate cellular functions and processes to specialized structures within cells. • Role of enzymes • Storage and transfer of genetic information Examples: replication, transcription, and translation ...
Transcription Translation 2017 p2.notebook
... A. Goal: Take a gene from DNA and make a mRNA copy B. Location: Nucleus (eukaryotic cell) C. Steps to Transcription: 1. Initiation: RNA Polymerase binds to the promoter (start sequence) of a gene on a DNA strand 2. Elongation: RNA Polymerase build a new RNA strand off the template str ...
... A. Goal: Take a gene from DNA and make a mRNA copy B. Location: Nucleus (eukaryotic cell) C. Steps to Transcription: 1. Initiation: RNA Polymerase binds to the promoter (start sequence) of a gene on a DNA strand 2. Elongation: RNA Polymerase build a new RNA strand off the template str ...