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Slide 1
Slide 1

... 1.5 Construct in both cases sequence logo and frequency plot. Can you identify (regulatory) sequence motifs? ...
Exercise 2: Sentence length Determine the distribution of words per
Exercise 2: Sentence length Determine the distribution of words per

... secret but also that they are all counted. In quantum cryptography, as in most long-distance data transmission, the information is carried by photons, the particles which compose light and other sorts of electromagnetic radiation. These particular photons, however, are manipulated in a special way. ...
DNase I (AMPD1) - Technical Bulletin - Sigma
DNase I (AMPD1) - Technical Bulletin - Sigma

... reactions should be run without adding reverse transcriptase to check for amplification of contaminating DNA. These precautions are especially recommended if PCR primers do not span an intron, if pseudogenes that lack the intron may be present in the target cells or tissue,1 or if the RNA will be us ...
Lecture 1: Molecular Biology
Lecture 1: Molecular Biology

... Transcription • Transcription is a complex process involving several steps and many proteins (enzymes) • RNA polymerase synthesizes a single strand of RNA against the DNA template strand (antisense strand), adding nucleotides to the 3’ end of the RNA chain • Initiation is regulated by transcription ...
Design and Operation of Large Scale RNA production v2
Design and Operation of Large Scale RNA production v2

... • Basic Synthetic Cycle Steps • The Detritylation Step (deblocking) • The Coupling (Activation) Step ...
DNA
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... How Proteins are made by the cell: There are 4 kinds of RNA. 3 of these are used in protein synthesis 1. Messenger RNA (mRNA) – carries an RNA version of a gene to a ribosome in order to make a protein. ...
Non-coding RNAs - Structural Biology Labs
Non-coding RNAs - Structural Biology Labs

... Genetic imprinting and shRNAs • Genetic imprinting is a process which results in expression on only one allele of gene, while the allele originating from the other parent is silenced • Process is somewhat similar to dosage compensation • The differences of expression from both alleles are due to di ...
DNA STRUCTURE (Sections 10.1 – 10.3)
DNA STRUCTURE (Sections 10.1 – 10.3)

... AP Biology Reading Guide for Chapter 10 Molecular Biology of the Gene This chapter has a LOT of information. In order to understand, learn, and remember all this information (and there's a lot), you'll need to get all you can out of class, and supplement it with both the review and the new material ...
RNA
RNA

... qPCR measures quantities of target sequences Targets can be DNA, cDNA or RNA DNA: present in a sample ? number of copies in a sample ? -> copy number analysis ...
Document
Document

... sequences. The ribosome has constantly read the mRNA in the 5' ---> 3' direction. The result is a growing chain of amino acids, all bonded together to make a polypeptide chain. When a codon with the nonsense sequence UAA, UAG (seen here), or UGA is exposed, that is a signal that translocation is to ...
Genes - University of Arizona | Ecology and Evolutionary Biology
Genes - University of Arizona | Ecology and Evolutionary Biology

... • Introns can interrupt ORFs. The introns are spliced out of the mRNA leaving only the exons which form a continuous ORF; but DNA sequences will still have the introns. Partial solution: look for sequences that often flank introns. Designing computer programs to seach complete genome sequences is a ...
Transcription and Translation
Transcription and Translation

... AG C C T G T GAAC UCGGACACUUG ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... control your characteristics? DNA contains instructions for all the proteins your body makes. Proteins, in turn, determine the structure and function of all your cells. What determines a protein’s structure? It begins with the sequence of amino acids that make up the protein. Instructions for making ...
CHAPTER 17 FROM GENE TO PROTEIN
CHAPTER 17 FROM GENE TO PROTEIN

... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
Class Notes
Class Notes

... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
BACTERIAL VIRUSES ("Bacteriophage") “Mein Gott!” They`ve got
BACTERIAL VIRUSES ("Bacteriophage") “Mein Gott!” They`ve got

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MGB_LNA_Substitutes
MGB_LNA_Substitutes

... The above melting curves of a molecular beacon (FAM-BHQ) show that the incorporation of 3 propynyl-dC bases into its hairpin region increase its melting temperature by 4.5°C. It is important to note that the effective increase of melting temperature per single nucleotide exchange is subject to varia ...
Chapter 4 powerpoint file
Chapter 4 powerpoint file

...  They function at an optimal pH and Temperature  They are denatured or deactivated if exposed to extreme pH and temperature  They only bind a specific molecule  They only perform one specific reaction  While they change the reactants into new products enzymes themselves are not changed during a ...
THE lac OPERON
THE lac OPERON

... lactose are present This explains how the lac operon is transcribed only when lactose is present.  BUT….. this does not explain why the operon is not transcribed when both glucose and lactose are present. ...
3.1 Class Notes Powerpoint
3.1 Class Notes Powerpoint

... protein that is used within a cell. Proteins are made up of hundreds of amino acids in a specific sequence. When they get “out of order’ a mutation occurs. ...
Chapter 18 PPT
Chapter 18 PPT

... • Histone acetylation seems to loosen chromatin structure, enhancing transcription. ...
apbio ch 17 study guide
apbio ch 17 study guide

... Many eukaryotic genes code for a set of closely related polypeptides in a process called alternative splicing. ...
Solid Tumor
Solid Tumor

... in proprietary Anchored Multiplex PCR (AMP)™-based enrichment. This chemistry enables detection of all fusions associated with the genes in this panel in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints. ...
Ribonucleic acids are found in both the nucleus and the cytoplasm
Ribonucleic acids are found in both the nucleus and the cytoplasm

... Ribonucleic acid (RNA) functions in converting genetic information from genes into the amino acid sequences of proteins. The three universal types of RNA include transfer RNA (tRNA), messenger RNA (mRNA), and ribosomal RNA (rRNA). Messenger RNA acts to carry genetic sequence information between DNA ...
The RNA polymerase factory: a robotic in vitro assembly platform for
The RNA polymerase factory: a robotic in vitro assembly platform for

... strand (7)], whereas other mechanisms have been proposed by comparing the conformations of protein domains in different crystals or by co-crystallizing polymerases in the presence of various substrates or cofactors (e.g. 8,9). It is important to recognize that X-ray structures can only provide indivi ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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