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Gene Expression - Phillips Scientific Methods
Gene Expression - Phillips Scientific Methods

... _____ GTP is used to attach the large subunit of the ribosome to the mRNA initiation complex. _____ The next tRNA matches its anti-codon to the codon of the “A” site. _____ Spliceosome adheres to snRNPs and excises introns while sealing exons into a continuous strand of mRNA. _____ Two GTPs are used ...
Document
Document

... 4. List, in order, the tRNA anticodons that are complementary to the mRNA sequence AUGCAUGCAAGUUAG. How many amino acids will be in the polypeptide that is initially formed when this mRNA sequence is translated? ...
transcription and rna
transcription and rna

...  factor (fifth subunit) Holoenzyme binds to promoter Initiates transcription  factor releases from core enzyme during transcription Transcription in Eukaryotes Eukaryotic polymerases Three different RNA polymerases Ten or more subunits Regulatory elements Eukaryotic promoters bind transcription fa ...
Replication, Transcription, and Translation
Replication, Transcription, and Translation

... nucleus and RNA polymerase binds to a specific section where a mRNA will be synthesized ...
BIO CH 13 Test Review
BIO CH 13 Test Review

... 8. RNA polymerase binds to DNA during transcription and separates the DNA strands. It then uses one strand of DNA as a template from which to assemble 9. nucleotides into a complementary strand of RNA. 10. Promoters are signals in the DNA molecule that show RNA polymerase exactly where to begin maki ...
No Slide Title
No Slide Title

... RNA Metabolism Transcription - process by which DNA (genetic info) gets made into RNA (mRNA, rRNA, or tRNA) by an RNA polymerase mRNA - messenger RNA - encodes the amino acid sequence of > 1 protein specified by a gene(s) rRNA - ribosomal RNA - constituents of ribosomes (proteins synthesized here) - ...
RNA Metabolism - Transcription
RNA Metabolism - Transcription

... RNA Metabolism Transcription - process by which DNA (genetic info) gets made into RNA (mRNA, rRNA, or tRNA) by an RNA polymerase mRNA - messenger RNA - encodes the amino acid sequence of > 1 protein specified by a gene(s) rRNA - ribosomal RNA - constituents of ribosomes (proteins synthesized here) - ...
Lac repressor - The i gene product of the lac operon is a
Lac repressor - The i gene product of the lac operon is a

... repressor which, in the active form binds to the operator, thereby blocking transcription. The repressor also has a binding site for inducer. Binding of IPTG, allolactose, or some other inducer at this site inactivates the repressor by vastly decreasing its affinity for DNA. Inactivating the repress ...
From DNA to Protein - Southington Public Schools
From DNA to Protein - Southington Public Schools

... in a few ways. It is a single strand, has ribose sugar as part of its backbone and it contains Uracil as one of its 4 nitrogen bases instead of Thymine. Remember it this way: U R single (You are Single). Uracil, Ribose and a Single strand. How does the information on DNA in the nucleus end up as a p ...
File - EUREKA! Science
File - EUREKA! Science

... Starting Transcription  Many elements work together to control the start of transcription ...
Lecture 3 - Transcription (student)
Lecture 3 - Transcription (student)

... *structural component of ribosome that is used ...
A Tale of Three Inferences
A Tale of Three Inferences

... statistical mechanical (hence concentration dependent) way. • Controversial: interaction among different transcription factor-binding events. ...
Prok transcription
Prok transcription

... 2. Gives regulatory opportunity (all cells have the same DNA but not the same genes are expressed) 3. In Eukaryotes the DNA is located in the nucleus and RNA transports the information out to the protein synthesis apparatus in the cytoplasm ...
The Central Dogma of Molecular Biology
The Central Dogma of Molecular Biology

...  2. the DNA codes for the production of messenger RNA (mRNA) during transcription  3. In eucaryotic cells, the mRNA is processed and migrates from the nucleus to the cytoplasm  4. Messenger RNA carries coded information to the ribosomes. The ribosomes “read” this information and use it for protei ...
Lecture 40_GeneRegulationI_transcriptional_control_RoadMap
Lecture 40_GeneRegulationI_transcriptional_control_RoadMap

... 7. Transcriptional regulation (e.g. LacI binding operator) vs post-transcriptional regulation (e.g. allolactose binding LacI) 8. Genetics (complementation testing) can help reveal molecular mechanism Major differences between prokaryotic and eukaryotic gene regulation 1. The Nucleus: Transcription a ...
Rad24 Interaction with Yeast RPA Table S4. Other novel putative
Rad24 Interaction with Yeast RPA Table S4. Other novel putative

... Ubiquitin C-terminal hydrolase that cleaves ubiquitinprotein fusions to generate monomeric ubiquitin ...
Overview of Transcription
Overview of Transcription

... Transition to the elongation complex involves partial dissociation of the holoenzyme. The sigma factor is left at the promoter complex, and the core RNA polymerase proceeds downstream. Conformational changes in the core enzyme result in the flap segment of subunit β clamping around the DNA, so that ...
Chapter_17_answers
Chapter_17_answers

... o Mediate binding of RNA polymerase and initiation of transcription o Transcription factors + RNA polymerase = transcription initiation complex  TATA box o Transcription factor binds here TATAAAA ATATTTT 2. Elongation  RNA polymerase moves down DNA sequence, unwinding it 10 – 20 bases at a time  ...
Topic 7 The Discovery of DNA & Its Roles
Topic 7 The Discovery of DNA & Its Roles

... 2’-OH group, and the nitrogenous bases are A, C, G, & Uracil ...
3.4: Transcription and Translation
3.4: Transcription and Translation

... carries codons / triplet of bases each coding for one amino acid; transfer / tRNA each have specific anticodon; triplet of bases for specific amino acid; tRNA carries specific amino acid; tRNA binds to ribosomes; to corresponding triplet base / codon; a second tRNA binds to next codon; two amino aci ...
File - Mrs. Badger`s Honors Biology Class
File - Mrs. Badger`s Honors Biology Class

... _____ 2. The main function of tRNA is to a. carry a message that, when translated, forms proteins. b. form a portion of ribosomes, a cell’s protein factories. c. string together complementary RNA and DNA strands. d. bring amino acids from the cytoplasm to the ribosomes. _____ 3. What is the term for ...
7.3 Transcription (AHL)
7.3 Transcription (AHL)

... 3. Termination: Eventually, the polymerase transcribes a terminator sequence, which signal the end of the transcription unit. Shortly thereafter, the RNA transcript is released and the polymerase detaches from DNA. ...
BCH 401G Lecture 44 Eukaryotic gene expression Andres
BCH 401G Lecture 44 Eukaryotic gene expression Andres

... 1). The activation of transcription is associated with multiple changes in the structure of the chromatin in the region being transcribed. 2). Although both positive and negative regulatory elements exist, positive regulatory mechanisms predominate in the systems characterized to date. 3). Finally, ...
Transcription
Transcription

... and a kinase activity that can phosphorylate the CTD tail of RNA polymerase II; it is also involved in repair of damage to DNA. Phosphorylation of the CTD by the kinase activity of TFIIH may be needed to release RNA polymerase to start transcription. ...
GENE to PROTEIN
GENE to PROTEIN

... • First codon decipher was UUU • There are 64 codons • A codon codes for only 1 amino acid The genetic code must have evolved very early in the history of life because it is nearly universal among living organisms. ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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