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Trends in Genetics 9:375. [pdf reprint 109 kb]
... p\�1529 (Ref. 2). In our experience, the method failed with pUC8-1, pUC8-2, pUC9-2, pUC12 and pUC18 (Ref. 2). The differ ent results obtained with very similar vectors, such as pUC18 and pUC19, are probably the result of sequence differences in the amino termini of the Lacla proteins they produce; ...
... p\�1529 (Ref. 2). In our experience, the method failed with pUC8-1, pUC8-2, pUC9-2, pUC12 and pUC18 (Ref. 2). The differ ent results obtained with very similar vectors, such as pUC18 and pUC19, are probably the result of sequence differences in the amino termini of the Lacla proteins they produce; ...
MCB Lecture 2 – Protein Metabolism
... o Some proteins fold spontaneously into the correct conformation as they are being translated. What are heat shock proteins? o They are synthesized in large amount on heat exposure. o They have an affinity for exposed hydrophobic patches of incompletely folded proteins and use ATP to fold them corre ...
... o Some proteins fold spontaneously into the correct conformation as they are being translated. What are heat shock proteins? o They are synthesized in large amount on heat exposure. o They have an affinity for exposed hydrophobic patches of incompletely folded proteins and use ATP to fold them corre ...
Identification of the Missing Links in Prokaryotic Pentose Oxidation
... as a sole carbon and energy source. At present, three main catabolic pathways have been described for pentoses. The first is present in Bacteria and uses isomerases, kinases, and epimerases to convert D- and L-arabinose (Ara) and D-xylose (Xyl) into D-xylulose 5-phosphate (Fig. 1A), which is further ...
... as a sole carbon and energy source. At present, three main catabolic pathways have been described for pentoses. The first is present in Bacteria and uses isomerases, kinases, and epimerases to convert D- and L-arabinose (Ara) and D-xylose (Xyl) into D-xylulose 5-phosphate (Fig. 1A), which is further ...
Identification of a novel testis‐specific gene and its potential roles in
... Aim: To identify and characterize a novel gene with potential roles in testis development and spermatogenesis. Methods: A cDNA microarray was constructed from a human testis large insert cDNA library and hybridized with probes of human or mouse adult and fetal testes. Differentially expressed genes ...
... Aim: To identify and characterize a novel gene with potential roles in testis development and spermatogenesis. Methods: A cDNA microarray was constructed from a human testis large insert cDNA library and hybridized with probes of human or mouse adult and fetal testes. Differentially expressed genes ...
Determination of the Binding Site-Size of the Protein
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
Infrared spectroscopic studies: from small molecules to large.
... Infrared (IR) spectroscopy has been around since the discovery of IR light by Friedrich Wilhelm Herschel in 1800. However, until 1940’s, IR studies involved only water and small organic molecules, because of the long measurement times and inaccessibility of the instruments. In 1940s came the first c ...
... Infrared (IR) spectroscopy has been around since the discovery of IR light by Friedrich Wilhelm Herschel in 1800. However, until 1940’s, IR studies involved only water and small organic molecules, because of the long measurement times and inaccessibility of the instruments. In 1940s came the first c ...
Nucleosome depletion at yeast terminators is not
... leading to the suggestion that intrinsic histone–DNA interactions appear to be more important for nucleosome depletion at terminator regions in vivo (13, 14). The conclusion that intrinsic histone–DNA interactions are important for nucleosome depletion at promoters and terminators is based on the si ...
... leading to the suggestion that intrinsic histone–DNA interactions appear to be more important for nucleosome depletion at terminator regions in vivo (13, 14). The conclusion that intrinsic histone–DNA interactions are important for nucleosome depletion at promoters and terminators is based on the si ...
Go ontology
... The information in the annotation file includes evidence information which serves as a source to validate /the annotation information. ...
... The information in the annotation file includes evidence information which serves as a source to validate /the annotation information. ...
Nucleolus: the fascinating nuclear body
... to Wnally form a central body associated with caps (Hadjiolova et al. 1995). In the caps are several proteins related to the RNA polymerase (pol) I transcription machinery such as UBF, close to Wbrillarin-containing caps. In the central body are proteins derived from the GC, some of which are progre ...
... to Wnally form a central body associated with caps (Hadjiolova et al. 1995). In the caps are several proteins related to the RNA polymerase (pol) I transcription machinery such as UBF, close to Wbrillarin-containing caps. In the central body are proteins derived from the GC, some of which are progre ...
Genes required for Lactococcus garvieae survival in a fish host
... selection will select against replication of mutant strains where a transposon-mediated disruption of genes related to virulence has occurred. Second, as each mutagenizing transposon carries ‘signature tags’ (DNA fragments consisting of a central variable region that is flanked by two invariant arms ...
... selection will select against replication of mutant strains where a transposon-mediated disruption of genes related to virulence has occurred. Second, as each mutagenizing transposon carries ‘signature tags’ (DNA fragments consisting of a central variable region that is flanked by two invariant arms ...
Applications of Recombinant DNA to Pathologic Diagnosis
... shown by Northern analysis. All patients lacked HPRT enzymlc activity ...
... shown by Northern analysis. All patients lacked HPRT enzymlc activity ...
chapter 3
... et al., 1987). This method can produce large amount of a specific DNA sequence from a complex DNA template in a simple enzymatic reaction. This method utilizes a DNA polymerase and two oligonucleotide primers to synthesize a specific DNA from a single stranded template sequence. The oligonucleotides ...
... et al., 1987). This method can produce large amount of a specific DNA sequence from a complex DNA template in a simple enzymatic reaction. This method utilizes a DNA polymerase and two oligonucleotide primers to synthesize a specific DNA from a single stranded template sequence. The oligonucleotides ...
Isolation and Purification of Nucleic Acids
... Knew that you could expose template DNA by boiling ds DNA to produce ss DNA Knew that you could use primers to initiate DNA synthesis Knew that a cheap, commercial enzyme was available (Klenow fragment of E. coli DNA polymerase) ...
... Knew that you could expose template DNA by boiling ds DNA to produce ss DNA Knew that you could use primers to initiate DNA synthesis Knew that a cheap, commercial enzyme was available (Klenow fragment of E. coli DNA polymerase) ...
Infectious Pancreatic Necrosis Virus genesig Standard Kit
... this genome segment overlaps the N-terminal region of VP2 and encodes the small nonstructural protein VP5. The smaller genomic segment, B, is 2784 nucleotides long and encodes VP1, the virion-associated RNA-dependent RNA polymerase. Transmission of this virus occurs via contact with infected bodily ...
... this genome segment overlaps the N-terminal region of VP2 and encodes the small nonstructural protein VP5. The smaller genomic segment, B, is 2784 nucleotides long and encodes VP1, the virion-associated RNA-dependent RNA polymerase. Transmission of this virus occurs via contact with infected bodily ...
Document
... The ribosome now advances one codon along the mRNA. EF-G is used in translocation in prokaryotes. EF-G-GTP binds the ribosome, GTP is hydrolyzed, and the ribosome moves one codon while the uncharged tRNA leaves the P site. In eukaryotes factor is called eEF-2. Release of the uncharged tRNA involves ...
... The ribosome now advances one codon along the mRNA. EF-G is used in translocation in prokaryotes. EF-G-GTP binds the ribosome, GTP is hydrolyzed, and the ribosome moves one codon while the uncharged tRNA leaves the P site. In eukaryotes factor is called eEF-2. Release of the uncharged tRNA involves ...
FREE Sample Here
... Ans: Meselson and Stahl grew bacteria in the presence of 15N, or “heavy” nitrogen. They rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined ...
... Ans: Meselson and Stahl grew bacteria in the presence of 15N, or “heavy” nitrogen. They rapidly replaced the 15N media with 14N, or light nitrogen. DNA was extracted at various time intervals during the growth of the bacteria, representing different stages of replication (generations). They examined ...
Gene clusters for β-lactam antibiotics and control of their expression
... the hydroxylation at C-3 that produces deacetylcephalosporin C (DAC). However, in cephamycin- and cephabacin-producing organisms, two different genes, cefE and cefF, encode enzymes that carry out these two sequential steps. Genes cefE and cefF encode proteins with about 70% identity in amino acids, ...
... the hydroxylation at C-3 that produces deacetylcephalosporin C (DAC). However, in cephamycin- and cephabacin-producing organisms, two different genes, cefE and cefF, encode enzymes that carry out these two sequential steps. Genes cefE and cefF encode proteins with about 70% identity in amino acids, ...
Full text in pdf - International Microbiology
... the hydroxylation at C-3 that produces deacetylcephalosporin C (DAC). However, in cephamycin- and cephabacin-producing organisms, two different genes, cefE and cefF, encode enzymes that carry out these two sequential steps. Genes cefE and cefF encode proteins with about 70% identity in amino acids, ...
... the hydroxylation at C-3 that produces deacetylcephalosporin C (DAC). However, in cephamycin- and cephabacin-producing organisms, two different genes, cefE and cefF, encode enzymes that carry out these two sequential steps. Genes cefE and cefF encode proteins with about 70% identity in amino acids, ...
NMR analysis of protein interactions
... Not all RRM motifs, however, are involved in RNA binding; interactions with protein have been observed as well [44]. Another abundant single-stranded RNA binding motif is the KH domain, reviewed in [43]. A solution structure has been determined for the KH domain of splicing factor SF1 in complex wit ...
... Not all RRM motifs, however, are involved in RNA binding; interactions with protein have been observed as well [44]. Another abundant single-stranded RNA binding motif is the KH domain, reviewed in [43]. A solution structure has been determined for the KH domain of splicing factor SF1 in complex wit ...
Analysis of the Nitrous Oxide Reduction Genes, nosZDFYL, of
... The structural gene, nosZ, for the monomeric N2O reductase has been cloned and sequenced from the denitrifying bacterium Achromobacter cycloclastes. The nosZ gene encodes a protein of 642 amino acid residues and the deduced amino acid sequence showed homology to the previously derived sequences for ...
... The structural gene, nosZ, for the monomeric N2O reductase has been cloned and sequenced from the denitrifying bacterium Achromobacter cycloclastes. The nosZ gene encodes a protein of 642 amino acid residues and the deduced amino acid sequence showed homology to the previously derived sequences for ...
DreamTaq DNA Polymerase, 5x500U
... of template DNA in the PCR mixture and the expected PCR product yield. If less than 10 copies of the template are present in the reaction, about 40 cycles are required. For higher template amounts, 25-35 cycles are sufficient. Final extension After the last cycle, it is recommended to incubate the P ...
... of template DNA in the PCR mixture and the expected PCR product yield. If less than 10 copies of the template are present in the reaction, about 40 cycles are required. For higher template amounts, 25-35 cycles are sufficient. Final extension After the last cycle, it is recommended to incubate the P ...
Handout
... wound healing to viral replication, proteases can be broadly lumped into two camps based on what’s in their ...
... wound healing to viral replication, proteases can be broadly lumped into two camps based on what’s in their ...
letters Solution structure of the DNA-binding domain of MafG
... subdivided into two groups: large Mafs (26–39 kDa), which have a putative transactivation domain at their N-termini, and small Mafs (17–18 kDa), which lack such a domain1–3 (Fig. 1a). Maf family proteins play important roles in cellular differentiation and morphogenesis, and their unique functions s ...
... subdivided into two groups: large Mafs (26–39 kDa), which have a putative transactivation domain at their N-termini, and small Mafs (17–18 kDa), which lack such a domain1–3 (Fig. 1a). Maf family proteins play important roles in cellular differentiation and morphogenesis, and their unique functions s ...
