RT-PCR Master Mix (2X)
... RNA compared to contaminating genomic DNA. For primers crossing an exon-exon border, PCR product should not be generated from genomic DNA. Be aware that common housekeeping genes such as β-actin or GAPDH have intron-less pseudogenes in many organisms. In those cases, it is important to have RNA whic ...
... RNA compared to contaminating genomic DNA. For primers crossing an exon-exon border, PCR product should not be generated from genomic DNA. Be aware that common housekeeping genes such as β-actin or GAPDH have intron-less pseudogenes in many organisms. In those cases, it is important to have RNA whic ...
Glycine-rich proteins as structural components of plant cell walls
... The primary structure of GRPs GRPs are characterized by their high content of glycine residues. GRPs, however, are not necessarily structural proteins, as RNA-binding proteins also have glycine-rich domains [7–9]. Since this article is focused on structural GRPs localized in the cell wall, only prot ...
... The primary structure of GRPs GRPs are characterized by their high content of glycine residues. GRPs, however, are not necessarily structural proteins, as RNA-binding proteins also have glycine-rich domains [7–9]. Since this article is focused on structural GRPs localized in the cell wall, only prot ...
Extracting and Explaining Biological Knowledge in Microarray Data
... (an example of such genes are shown in the first column in Table 2), we reclustered them using terms in the Gene Ontology (the GO:nnnnnnn labels in the right column in Table 2) into groups of similarly described genes, for example, genes that control signal transduction, or genes associated with tra ...
... (an example of such genes are shown in the first column in Table 2), we reclustered them using terms in the Gene Ontology (the GO:nnnnnnn labels in the right column in Table 2) into groups of similarly described genes, for example, genes that control signal transduction, or genes associated with tra ...
Three-Point Binding Model
... template synthesis): Ribosome holds pieces together Ribosome is cellular “workbench” ...
... template synthesis): Ribosome holds pieces together Ribosome is cellular “workbench” ...
local copy pdf
... ing RNA chains that were too big to diffuse of different compounds and short peptides help define the chemistry that must have back out. A year later, Szostak and his gradu- to the mix. “Nothing worked,” she says. “It been involved at some level to get a selfate student Ting Zhu found that adding ex ...
... ing RNA chains that were too big to diffuse of different compounds and short peptides help define the chemistry that must have back out. A year later, Szostak and his gradu- to the mix. “Nothing worked,” she says. “It been involved at some level to get a selfate student Ting Zhu found that adding ex ...
Identification of Virgibacillus species using 16S rRNA gene Sequence
... A comparison of the 16s rRNA gene sequence of the test strain with the non -redundant collection (Genbank, DDBJ, EMBL & PDB) of sequences was performed using BLAST . A number of sequences of the genus Virgibacillus aligned with 16S rRNA gene sequence of test strain. We then developed a multiple sequ ...
... A comparison of the 16s rRNA gene sequence of the test strain with the non -redundant collection (Genbank, DDBJ, EMBL & PDB) of sequences was performed using BLAST . A number of sequences of the genus Virgibacillus aligned with 16S rRNA gene sequence of test strain. We then developed a multiple sequ ...
Amino Acid Limitation Induces the Amino Acid
... rat samples had a much larger expression rate within the first 4 hours. Most of the declines were very rapid and sudden for both the rat and human samples. At the end of 24 hours, every single sample still had a higher expression rate than what they started with. They also always had higher expressi ...
... rat samples had a much larger expression rate within the first 4 hours. Most of the declines were very rapid and sudden for both the rat and human samples. At the end of 24 hours, every single sample still had a higher expression rate than what they started with. They also always had higher expressi ...
tRNA & Ribosomes
... There is a selenocysteine tRNA that differs from other tRNAs, e.g., in having a slightly longer acceptor stem & a unique modified base in the anticodon loop. tRNASec is loaded with serine via Seryl-tRNA Synthetase. The serine moiety is then converted to selenocysteine by another enzyme, in a reactio ...
... There is a selenocysteine tRNA that differs from other tRNAs, e.g., in having a slightly longer acceptor stem & a unique modified base in the anticodon loop. tRNASec is loaded with serine via Seryl-tRNA Synthetase. The serine moiety is then converted to selenocysteine by another enzyme, in a reactio ...
Genome Sequence of an Extremely Halophilic Archaeon
... was the finding of an extremely acidic complement of encoded proteins, which is likely directly related to protein function in its hypersaline (>4M KCl) cytoplasm (11). Calculated isoelectric points (pIs) for predicted proteins showed an average pI of approx 5, a prediction confirmed by proteomic an ...
... was the finding of an extremely acidic complement of encoded proteins, which is likely directly related to protein function in its hypersaline (>4M KCl) cytoplasm (11). Calculated isoelectric points (pIs) for predicted proteins showed an average pI of approx 5, a prediction confirmed by proteomic an ...
Platinum DNA polymerases
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
RNA Isolation and Technology Applications
... Techniques for RNA analysis: Microarrays for Gene Expression Profiling • Microarrays enable researchers to examine the expression levels of thousands of genes in a single experiment • As many as 50,000 or more unique DNA sequences are spotted on to a glass slide – Each spot represents one unique, k ...
... Techniques for RNA analysis: Microarrays for Gene Expression Profiling • Microarrays enable researchers to examine the expression levels of thousands of genes in a single experiment • As many as 50,000 or more unique DNA sequences are spotted on to a glass slide – Each spot represents one unique, k ...
2. Identifying coding sequences (genes)
... - RSPs: single nucleotide polymorphism may cause a loss or gain in a restriction site generating an RSP. Used in identifying carriers for some disease causing genes. - VNTR: use of PCR or Southern blot hybridization to identify differences in the number of microsatellite tandem repeats. ...
... - RSPs: single nucleotide polymorphism may cause a loss or gain in a restriction site generating an RSP. Used in identifying carriers for some disease causing genes. - VNTR: use of PCR or Southern blot hybridization to identify differences in the number of microsatellite tandem repeats. ...
Locked Nucleic Acid - LNA™
... Exiqon Products: Products are for research use only and not for diagnostic or therapeutic use. The products may be used only for the buyer’s internal research purposes and not for commercial use. The buyer may not resell products in their original or any modified form. The purchase of products does ...
... Exiqon Products: Products are for research use only and not for diagnostic or therapeutic use. The products may be used only for the buyer’s internal research purposes and not for commercial use. The buyer may not resell products in their original or any modified form. The purchase of products does ...
Ch. 1 Plasmids
... are restricted to genes that encode proteins. cDNA sequences help "decode" the genome sequence by identifying specific protein-coding regions. For example, the genome of the soil nematode C. elegans is thought to encode approximately 19,000 genes. However, there is a lot of DNA that is not devoted t ...
... are restricted to genes that encode proteins. cDNA sequences help "decode" the genome sequence by identifying specific protein-coding regions. For example, the genome of the soil nematode C. elegans is thought to encode approximately 19,000 genes. However, there is a lot of DNA that is not devoted t ...
Comparative analyses of Saccharomyces cerevisiae RNAs using
... Fig. 1. Estimation of RNA concentration using RNA Nano Assay and EtBr-stained agarose electrophoresis. A: Concentration calibration curve of diluted RNAs performed on Nano Assay. Numbers within the graph indicate amounts of analysed RNA in ng. Arrows indicate the range of concentrations reproducibly ...
... Fig. 1. Estimation of RNA concentration using RNA Nano Assay and EtBr-stained agarose electrophoresis. A: Concentration calibration curve of diluted RNAs performed on Nano Assay. Numbers within the graph indicate amounts of analysed RNA in ng. Arrows indicate the range of concentrations reproducibly ...
Comparison of good-and bad-quality cork: application of high
... Cork is one of the most valuable non-wood forest products and plays an important role in Mediterranean economies. The production of high-quality cork is dependent on both genome and environment, posing constraints on the industry because an ever-growing amount of bad-quality cork (BQC) development h ...
... Cork is one of the most valuable non-wood forest products and plays an important role in Mediterranean economies. The production of high-quality cork is dependent on both genome and environment, posing constraints on the industry because an ever-growing amount of bad-quality cork (BQC) development h ...
Week
... 3. Given the genotype of a daughter cell, diagram how it was produced by mitosis or meiosis ...
... 3. Given the genotype of a daughter cell, diagram how it was produced by mitosis or meiosis ...
Chen-6-Translation
... • Initiation-- once per protein it gets the system in motion • Elongation-- repeated for each codon in the mRNA making a peptide bond • Termination-- finishes and releases the newly synthesized protein ...
... • Initiation-- once per protein it gets the system in motion • Elongation-- repeated for each codon in the mRNA making a peptide bond • Termination-- finishes and releases the newly synthesized protein ...
Translation
... As mRNA binds, IF-3 helps to correctly position the complex such that the tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ri ...
... As mRNA binds, IF-3 helps to correctly position the complex such that the tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ri ...
PHM 281N Pharmaceutical Biochemistry II
... *What descriptors are used to refer to the DNA strand that is copied in transcription? The other DNA strand? *How are transcribed regions of DNA recognized in prokaryotes? In eukaryotes? *What is the function of the sigma factor in prokaryote RNA polymerase? What carries out the corresponding functi ...
... *What descriptors are used to refer to the DNA strand that is copied in transcription? The other DNA strand? *How are transcribed regions of DNA recognized in prokaryotes? In eukaryotes? *What is the function of the sigma factor in prokaryote RNA polymerase? What carries out the corresponding functi ...
Use of Gene Replacement Transformation to Elucidate
... (GEEVERet al. 1989). T h e cluster includes five structural genes and two regulatory genes. Three of the structural genes, qa-2, qa-3 and qa-4 are involved in the first three steps of quinic acidcatabolism (GILESet a1 1985). A fourthgene, qa-y, is hypothesized to encode a quinic acid permease (GEEVE ...
... (GEEVERet al. 1989). T h e cluster includes five structural genes and two regulatory genes. Three of the structural genes, qa-2, qa-3 and qa-4 are involved in the first three steps of quinic acidcatabolism (GILESet a1 1985). A fourthgene, qa-y, is hypothesized to encode a quinic acid permease (GEEVE ...
Departamento de Clínica Médica
... Leonardo Domingues de Araújo e Profa. Dra. Margaret de Castro ...
... Leonardo Domingues de Araújo e Profa. Dra. Margaret de Castro ...
Biochemistry, Cell and Molecular Biology Test Practice Book
... the subject who are on undergraduate and graduate faculties in different types of institutions and in different regions of the United States and Canada. In selecting members for each committee, the GRE Program seeks the advice of appropriate professional associations in the subject. The content and ...
... the subject who are on undergraduate and graduate faculties in different types of institutions and in different regions of the United States and Canada. In selecting members for each committee, the GRE Program seeks the advice of appropriate professional associations in the subject. The content and ...
extracellular polysaccharide colanic acid. cluster responsible for
... noted that the CA repeat unit has a three-sugar side branch and it is possible that a mutation in one of the side branch transferases would not affect polymerization of the incomplete repeat unit. Thus, although it is not proven, it is still quite possible that orf20 is part of the CA gene cluster, ...
... noted that the CA repeat unit has a three-sugar side branch and it is possible that a mutation in one of the side branch transferases would not affect polymerization of the incomplete repeat unit. Thus, although it is not proven, it is still quite possible that orf20 is part of the CA gene cluster, ...
EF-G-GTP
... complex such that tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA, helping to position the 30S ribosomal subunit in relation to the initiation codon. ...
... complex such that tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA, helping to position the 30S ribosomal subunit in relation to the initiation codon. ...