Use of lac regulatory elements for gene expression in
... been expressed in lactobacilli with different constitutive promoters, with or without secretion signals [11, 15, 17, 22, 32, 37]. However, more efficient expression systems have been developed for Lactococcus lactis that used different marker genes and regulatory elements [9]. Recently, a very remar ...
... been expressed in lactobacilli with different constitutive promoters, with or without secretion signals [11, 15, 17, 22, 32, 37]. However, more efficient expression systems have been developed for Lactococcus lactis that used different marker genes and regulatory elements [9]. Recently, a very remar ...
A phage library and two cosmid libraries were
... were used for transduction into E. coli 49OA. Screening of cosmid libraries Cosmid containing bacteria (E. coli HB 101 for library I, E. coli 49OA for library II) were plated directly onto nitrocellulose filters placed on L-agar plates (150 mm 0) at a density of 5-1Ox1O3 colonies/plate. A total of 2 ...
... were used for transduction into E. coli 49OA. Screening of cosmid libraries Cosmid containing bacteria (E. coli HB 101 for library I, E. coli 49OA for library II) were plated directly onto nitrocellulose filters placed on L-agar plates (150 mm 0) at a density of 5-1Ox1O3 colonies/plate. A total of 2 ...
EF-Tu PROTEIN DOMAINS
... In this work, we focused on the structure-function relationships between EF-Tu proteins and their domains. The domain effect had been before our studies tested mainly by truncated EF-Tu forms lacking one or two domains. In contrast, we decided to study the properties of individual domains within ful ...
... In this work, we focused on the structure-function relationships between EF-Tu proteins and their domains. The domain effect had been before our studies tested mainly by truncated EF-Tu forms lacking one or two domains. In contrast, we decided to study the properties of individual domains within ful ...
DNA Replication and Telomere Maintenance
... origins • Analysis by two-dimensional agarose gel electrophoresis. • Other techniques allow detection of the start site for DNA synthesis at the nucleotide level. • Data suggest that there is a single defined start point. ...
... origins • Analysis by two-dimensional agarose gel electrophoresis. • Other techniques allow detection of the start site for DNA synthesis at the nucleotide level. • Data suggest that there is a single defined start point. ...
Eukaryotic-type protein kinases in Streptomyces
... others in differentiation of S. coelicolor still remains speculative (Umeyama et al., 2002; Petrickova et al., 2000). The functions of some ESTPKs have been investigated in other streptomycete species: Pkg2 is required for aerial mycelium formation in Streptomyces granaticolor (Nadvornik et al., 199 ...
... others in differentiation of S. coelicolor still remains speculative (Umeyama et al., 2002; Petrickova et al., 2000). The functions of some ESTPKs have been investigated in other streptomycete species: Pkg2 is required for aerial mycelium formation in Streptomyces granaticolor (Nadvornik et al., 199 ...
CHAPTER 8 Recombinant DNA Technology
... c. DNA polymerase I makes new DNA fragments, and DNA ligase connects the new DNA fragments to make a complete chain. d. The resulting cDNA is a double-stranded copy of the starting ...
... c. DNA polymerase I makes new DNA fragments, and DNA ligase connects the new DNA fragments to make a complete chain. d. The resulting cDNA is a double-stranded copy of the starting ...
Lecture II - Baylor School of Engineering & Computer Science
... #Here’s one way to declare an array @bases = (‘A’,’C’,’G’,’T’); #Now print each element of the array in a row print “\nHere are all of the bases: “ , @bases; #This prints out: ‘Here are all of the bases: ACGT’ #But, you can print them out with spaces in between print “\nHere they are with spaces” , ...
... #Here’s one way to declare an array @bases = (‘A’,’C’,’G’,’T’); #Now print each element of the array in a row print “\nHere are all of the bases: “ , @bases; #This prints out: ‘Here are all of the bases: ACGT’ #But, you can print them out with spaces in between print “\nHere they are with spaces” , ...
Gustav Fischer Verlag Stuttgart • New York
... et a l . , 1982), of cell w a l l composition (Kandier, 1982) a n d membrane structure (review by Langworthy et a l . , 1982). In order to study phylogenetic relationships and evolution of different organisms it is desirable to compare cellular components that: (1) are present i n all organisms unde ...
... et a l . , 1982), of cell w a l l composition (Kandier, 1982) a n d membrane structure (review by Langworthy et a l . , 1982). In order to study phylogenetic relationships and evolution of different organisms it is desirable to compare cellular components that: (1) are present i n all organisms unde ...
Lecture 13 Lytic vs. Lysogenic cycles:
... Lytic cycle = the phage attaches to the cell wall of the bacteria and injects DNA into the cell. The DNA forms into a circle and recruits the host machinery to produce more and more phage components. Eventually the cell wall ruptures and all the new phage come out after being assembled and having DN ...
... Lytic cycle = the phage attaches to the cell wall of the bacteria and injects DNA into the cell. The DNA forms into a circle and recruits the host machinery to produce more and more phage components. Eventually the cell wall ruptures and all the new phage come out after being assembled and having DN ...
Vectors and Libraries
... are restricted to genes that encode proteins. cDNA sequences help "decode" the genome sequence by identifying specific protein-coding regions. For example, the genome of the soil nematode C. elegans is thought to encode approximately 19,000 genes. However, there is a lot of DNA that is not devoted t ...
... are restricted to genes that encode proteins. cDNA sequences help "decode" the genome sequence by identifying specific protein-coding regions. For example, the genome of the soil nematode C. elegans is thought to encode approximately 19,000 genes. However, there is a lot of DNA that is not devoted t ...
Full Text - Genome Biology and Evolution
... active ERVs in bovine conceptuses during the implantation period. Recent advances in DNA sequencing technology have enabled us to process increasingly large numbers of nucleotide sequences (Schuster 2008). Previously, we used microarrays to determine genes involved in the process of implantation in ...
... active ERVs in bovine conceptuses during the implantation period. Recent advances in DNA sequencing technology have enabled us to process increasingly large numbers of nucleotide sequences (Schuster 2008). Previously, we used microarrays to determine genes involved in the process of implantation in ...
Document
... other genes, the transcriptionally active domain is separated from SREBP by proteolytic cleavage. When cholesterol levels are high, SREBPs are inactive, secured to ER in a complex with another protein called SREBP cleavage- activating protein (SCAP). SCAP binds cholesterol and a number of other ...
... other genes, the transcriptionally active domain is separated from SREBP by proteolytic cleavage. When cholesterol levels are high, SREBPs are inactive, secured to ER in a complex with another protein called SREBP cleavage- activating protein (SCAP). SCAP binds cholesterol and a number of other ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... The mfold package can also be used to assign promiscuity to any nucleotide within a user defined energy window, and structures can be colour coded with this information (14). P-num describes the promiscuity of a nucleotide, which is the number of different base pairs for a given nucleotide in all fol ...
... The mfold package can also be used to assign promiscuity to any nucleotide within a user defined energy window, and structures can be colour coded with this information (14). P-num describes the promiscuity of a nucleotide, which is the number of different base pairs for a given nucleotide in all fol ...
Genetic backgrounds of each Escherichia coli strain used
... Δ(lacZ)M15: This E. coli strain carries the lacZ deletion mutant which contains the ω-peptide: a mutant βgalactosidase derived from the M15 strain of E. coli that has its N-terminal residues 11—41 deleted and is unable to form a tetramer so it is inactive. The plasmids used in transformation process ...
... Δ(lacZ)M15: This E. coli strain carries the lacZ deletion mutant which contains the ω-peptide: a mutant βgalactosidase derived from the M15 strain of E. coli that has its N-terminal residues 11—41 deleted and is unable to form a tetramer so it is inactive. The plasmids used in transformation process ...
BMC Genomics Expansion of the Bactericidal/Permeability Increasing-like (BPI-like) protein locus in cattle
... sequence similarity, exon segmentation and predicted secondary structure [10,11]. All but two of these are found as a gene cluster at a single locus on human chromosome 20 or the syntenic region of mouse chromosome 2. The similarity of the products of these genes to BPI and LBP, their expression in ...
... sequence similarity, exon segmentation and predicted secondary structure [10,11]. All but two of these are found as a gene cluster at a single locus on human chromosome 20 or the syntenic region of mouse chromosome 2. The similarity of the products of these genes to BPI and LBP, their expression in ...
Proteins and Their Synthesis
... Answer: It would be unlikely that the 23S rRNA of prokaryotes would be able to substitute for the 28S rRNA of eukaryotes. Its different size implies a different folding pattern which would affect its affinity for protein components of the 60S subunit. Also, the 60S subunit of eukaryotes contains 49 ...
... Answer: It would be unlikely that the 23S rRNA of prokaryotes would be able to substitute for the 28S rRNA of eukaryotes. Its different size implies a different folding pattern which would affect its affinity for protein components of the 60S subunit. Also, the 60S subunit of eukaryotes contains 49 ...
The Nucleolus under Stress
... posttranslational modification (Banerjee et al., 2005), while hepatitis C virus stimulates RNA Pol I activity, thereby promoting liver carcinogenesis (Kao et al., 2004). Despite the consensus that stress-dependent regulation of pre-rRNA synthesis mainly occurs by influencing the transcriptional rate ...
... posttranslational modification (Banerjee et al., 2005), while hepatitis C virus stimulates RNA Pol I activity, thereby promoting liver carcinogenesis (Kao et al., 2004). Despite the consensus that stress-dependent regulation of pre-rRNA synthesis mainly occurs by influencing the transcriptional rate ...
DNA Science - University of Missouri
... •We eat food which is composed of chemicals called proteins, ...
... •We eat food which is composed of chemicals called proteins, ...
475 S07 background questions
... 107. Distinguish between transposons and retrotransposons. 108. Describe the structure and location of Alu elements in primate genomes. 109. Describe the structure and possible function of simple sequence DNA. 110. Using the genes for rRNA as an example, explain how multigene families of identical g ...
... 107. Distinguish between transposons and retrotransposons. 108. Describe the structure and location of Alu elements in primate genomes. 109. Describe the structure and possible function of simple sequence DNA. 110. Using the genes for rRNA as an example, explain how multigene families of identical g ...
2-Oxoacid dehydrogenase multienzyme complexes
... coding regions overlap by 4 nt, with the ATG start codon of ORF2 sharing bases with the ORF1 TGA stop codon ; ORF2 and ORF3 are in-frame with each other, separated by three bases ; ORF3 and ORF4 (the DHLipDH gene) share a single nucleotide, with the TTA stop codon of ORF3 overlapping the ATG start c ...
... coding regions overlap by 4 nt, with the ATG start codon of ORF2 sharing bases with the ORF1 TGA stop codon ; ORF2 and ORF3 are in-frame with each other, separated by three bases ; ORF3 and ORF4 (the DHLipDH gene) share a single nucleotide, with the TTA stop codon of ORF3 overlapping the ATG start c ...
Lab 1 - DNA Isolation from Drosophila melanogaster (Fly DNA Mini
... 1. Freeze 50 Drosophila larvae or flies in a microcentrifuge Tube P (pellet) on dry ice or wet ice to slow down the fly movement. ...
... 1. Freeze 50 Drosophila larvae or flies in a microcentrifuge Tube P (pellet) on dry ice or wet ice to slow down the fly movement. ...
Reconstruction of Amino Acid Biosynthesis Pathways from the
... although this organism also seems to have all the amino acid biosynthesis pathways as shown by Tatusov et al. (1996) as well. This may suggest the possibility that an enzyme in H. influenzae plays multiple functional roles that are taken by different enzymes in E. coli. For example, E. coli has tyro ...
... although this organism also seems to have all the amino acid biosynthesis pathways as shown by Tatusov et al. (1996) as well. This may suggest the possibility that an enzyme in H. influenzae plays multiple functional roles that are taken by different enzymes in E. coli. For example, E. coli has tyro ...
PowerPoint 演示文稿
... little hands on time Relative ease of transfection Best for: Screening siRNA sequences or when the price of chemical siRNA synthesis is an obstacle • Not suitable for: Long term studies or studies that require large amounts of a single siRNA sequence ...
... little hands on time Relative ease of transfection Best for: Screening siRNA sequences or when the price of chemical siRNA synthesis is an obstacle • Not suitable for: Long term studies or studies that require large amounts of a single siRNA sequence ...
Suppression mechanisms
... cdc2-3w wee1-50 double mutant. The mik1 gene encodes a protein kinase that directly phosphorylates Cdc2 on Tyr15, inhibiting Cdc2 activity. Phosphorylation is a common regulatory modification in vivo, but other types of modifications have also been identified by suppressors. RAM1, for example, was i ...
... cdc2-3w wee1-50 double mutant. The mik1 gene encodes a protein kinase that directly phosphorylates Cdc2 on Tyr15, inhibiting Cdc2 activity. Phosphorylation is a common regulatory modification in vivo, but other types of modifications have also been identified by suppressors. RAM1, for example, was i ...