Press Release
... EMBL is Europe’s flagship laboratory for the life sciences, with more than 80 independent groups covering the spectrum of molecular biology. EMBL is international, innovative and interdisciplinary – its 1800 employees, from many nations, operate across five sites: the main laboratory in Heidelberg, ...
... EMBL is Europe’s flagship laboratory for the life sciences, with more than 80 independent groups covering the spectrum of molecular biology. EMBL is international, innovative and interdisciplinary – its 1800 employees, from many nations, operate across five sites: the main laboratory in Heidelberg, ...
Localized hypermutation and associated gene losses in legume
... ened constraints on protein sequences, rather than being indic(Supplemental Fig. S2B). All the repeats are species-specific, which ative of positive selection on multiple adjacent genes. suggests that these minisatellite-like sequences have a high turnWe may have slightly overestimated the divergenc ...
... ened constraints on protein sequences, rather than being indic(Supplemental Fig. S2B). All the repeats are species-specific, which ative of positive selection on multiple adjacent genes. suggests that these minisatellite-like sequences have a high turnWe may have slightly overestimated the divergenc ...
Transcriptional regulation of mammalian autophagy at a glance
... referred to as p53) has been described to inhibit mTORC1 and thus to activate autophagy, several studies have shown that cytoplasmic p53 is a potent inhibitor of autophagy. The mechanisms for this inhibition are largely unknown (Green and Kroemer, 2009); however, post-transcriptional downregulation ...
... referred to as p53) has been described to inhibit mTORC1 and thus to activate autophagy, several studies have shown that cytoplasmic p53 is a potent inhibitor of autophagy. The mechanisms for this inhibition are largely unknown (Green and Kroemer, 2009); however, post-transcriptional downregulation ...
Uniikki kuitu
... It also allows access to many analytical tools for the identification of proteins, the analysis of their sequence and the prediction of their tertiary structure. ExPASy also offers you many documents relevant to these field of research and you will find from the servers, links to most relevant sourc ...
... It also allows access to many analytical tools for the identification of proteins, the analysis of their sequence and the prediction of their tertiary structure. ExPASy also offers you many documents relevant to these field of research and you will find from the servers, links to most relevant sourc ...
Cell-Free Protein Expression Systems
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
Mechanisms for Creation of “Original Ancestor Genes”
... Fig. 8. New gene creation from original (SNS)n gene. New genes were easily produced from original (SNS)n genes through two routes as similarly as those described in Fig. 6, one is from sense sequence and the other is from antisense sequence of the original gene. “Original ancestor genes” could be pr ...
... Fig. 8. New gene creation from original (SNS)n gene. New genes were easily produced from original (SNS)n genes through two routes as similarly as those described in Fig. 6, one is from sense sequence and the other is from antisense sequence of the original gene. “Original ancestor genes” could be pr ...
View PDF - Sutro Biopharma, Inc.
... protein synthesis — are commonly used because of their ease of preparation and relatively high productivity. Though often considered a ‘black-box’, genome and process engineered cell-free extracts allow exquisite design and control. They contain the necessary components for transcription (template D ...
... protein synthesis — are commonly used because of their ease of preparation and relatively high productivity. Though often considered a ‘black-box’, genome and process engineered cell-free extracts allow exquisite design and control. They contain the necessary components for transcription (template D ...
The home stretch, a first analysis of the nearly completed genome of
... shotgun-sequencing of the entire genome and The University of Texas-Houston Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the genome when data from both of these groups are combined. Previous work had suggested that the two chr ...
... shotgun-sequencing of the entire genome and The University of Texas-Houston Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the genome when data from both of these groups are combined. Previous work had suggested that the two chr ...
TruSeq™ Sample Preparation Best Practices and Troubleshooting
... ` Small differences in volumes (±0.5 μl) can sometimes give rise to very large differences in cluster numbers (~100,000). ` Small volume pipetting can be a source of potential error in protocols that require generation of standard curves, such as PicoGreen assays or qPCR, or those that require sm ...
... ` Small differences in volumes (±0.5 μl) can sometimes give rise to very large differences in cluster numbers (~100,000). ` Small volume pipetting can be a source of potential error in protocols that require generation of standard curves, such as PicoGreen assays or qPCR, or those that require sm ...
Gene Section VRK1 (Vaccinia-related kinase 1) Atlas of Genetics and Cytogenetics
... In this translocation the breakpoint occurs in a DNAseI hypersensitive site located between VRK1 and BCL11B genes; but the structure, or expression, of VRK1 does not appear to be affected. In this translocation there is a dysregulation of TLX3 and NKX2-5 homeobox genes (both on chromosome 5). ...
... In this translocation the breakpoint occurs in a DNAseI hypersensitive site located between VRK1 and BCL11B genes; but the structure, or expression, of VRK1 does not appear to be affected. In this translocation there is a dysregulation of TLX3 and NKX2-5 homeobox genes (both on chromosome 5). ...
8679821 - Southern Illinois University System
... The invention also provides methods of protecting against bacterial contamination. A method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention include ...
... The invention also provides methods of protecting against bacterial contamination. A method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention include ...
Lysines 72, 80 and 213 and aspartic acid 210 of the
... Site-directed mutagenesis of the Lactococcus lactis lacR gene was performed to identify residues in the LacR repressor that are involved in the induction of lacABCDFEGX operon expression by tagatose-6-phosphate. A putative inducer binding domain located near the C-terminus was previously postulated ...
... Site-directed mutagenesis of the Lactococcus lactis lacR gene was performed to identify residues in the LacR repressor that are involved in the induction of lacABCDFEGX operon expression by tagatose-6-phosphate. A putative inducer binding domain located near the C-terminus was previously postulated ...
Chromatin Dynamics during Lytic Infection with Herpes Simplex
... for a total of 200 bp and ~2 turns. The linear chain of nucleosomes is then folded into complex higher order structures to form the chromatin fibers. Chromatin is essential to compact the long eukaryotic genomic DNA to fit within the cell nucleus. For example, the more than 1m of human genomic DNA i ...
... for a total of 200 bp and ~2 turns. The linear chain of nucleosomes is then folded into complex higher order structures to form the chromatin fibers. Chromatin is essential to compact the long eukaryotic genomic DNA to fit within the cell nucleus. For example, the more than 1m of human genomic DNA i ...
FOG1 recruits the NuRD repressor complex to mediate
... suggesting that histone deacetylation once initiated might spread across the GATA-2 locus independent of GATA-1. We next examined whether FOG-1 binding by GATA-1 is required for GATA-1 occupancy at the GATA-2 and c-kit genes. As measured by ChIP, levels of GATA-1(V205M)-ER at the GATA-2 and c-kit lo ...
... suggesting that histone deacetylation once initiated might spread across the GATA-2 locus independent of GATA-1. We next examined whether FOG-1 binding by GATA-1 is required for GATA-1 occupancy at the GATA-2 and c-kit genes. As measured by ChIP, levels of GATA-1(V205M)-ER at the GATA-2 and c-kit lo ...
Applied Environmnetal Microbiology
... fivefold in 10 mM Tris-HCl (pH 8.0) buffer containing 1 mM phenylmethylsulfonyl fluoride, and the cells were disrupted by ultrasonic treatment until lysis was complete. The lysates were then centrifuged at 10,000 3 g to collect the inclusion bodies, and, finally, the pellet was suspended at 1/10 the ...
... fivefold in 10 mM Tris-HCl (pH 8.0) buffer containing 1 mM phenylmethylsulfonyl fluoride, and the cells were disrupted by ultrasonic treatment until lysis was complete. The lysates were then centrifuged at 10,000 3 g to collect the inclusion bodies, and, finally, the pellet was suspended at 1/10 the ...
Chapter 10 - People Server at UNCW
... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
Avian Infectious Bronchitis Virus (IBV) genesig Standard Kit
... recommend the use of a one step qRT-PCR protocol. One step qRT-PCR combines the reverse transcription and real-time PCR reaction in a simple closed tube protocol. This saves significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because ...
... recommend the use of a one step qRT-PCR protocol. One step qRT-PCR combines the reverse transcription and real-time PCR reaction in a simple closed tube protocol. This saves significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because ...
Answers - Study of Life
... Insulin injected by diabetics to control blood sugar levels is derived from bacteria whose DNA has been modified by the addition of the human gene for insulin, which is then produced by the prokaryotes. This is an example of: A. acid therapy B. cloning C. genetic engineering D. gene therapy E. pluri ...
... Insulin injected by diabetics to control blood sugar levels is derived from bacteria whose DNA has been modified by the addition of the human gene for insulin, which is then produced by the prokaryotes. This is an example of: A. acid therapy B. cloning C. genetic engineering D. gene therapy E. pluri ...
Relationship between codon biased genes, microarray expression
... from laboratory-grown cultures. High mRNA amounts were observed in 32?5 % of PHE genes and in 64 % of the 25 genes with the highest CAI values. However, high relative amounts of RNA were also detected in 10?4 % of non-PHE genes, such as those encoding fatty acid metabolism enzymes and proteases, sug ...
... from laboratory-grown cultures. High mRNA amounts were observed in 32?5 % of PHE genes and in 64 % of the 25 genes with the highest CAI values. However, high relative amounts of RNA were also detected in 10?4 % of non-PHE genes, such as those encoding fatty acid metabolism enzymes and proteases, sug ...
Full Text - PDF
... Following the T cell activation, cytotoxic T lymphocyte antigen-4 (CTLA-4), a negative regulatory molecule, will be expressed on T cells 1,2. CTLA-4 is a homolog of CD28 but binds B-7 molecules with greater affinity. Inhibitory signals of this molecule inhibit cell cycle progression and IL-2 product ...
... Following the T cell activation, cytotoxic T lymphocyte antigen-4 (CTLA-4), a negative regulatory molecule, will be expressed on T cells 1,2. CTLA-4 is a homolog of CD28 but binds B-7 molecules with greater affinity. Inhibitory signals of this molecule inhibit cell cycle progression and IL-2 product ...
Unveiling the Transcriptome using High
... Genome, Transcriptome, Proteome Schematic illustration of a eukaryotic cell ...
... Genome, Transcriptome, Proteome Schematic illustration of a eukaryotic cell ...
Sugar Transport in (Hyper-)Thermophilic Archaea
... Sonja M. Koning, Sonja-Verena Albers, Wil N. Konings and Arnold J. M. Driessen ...
... Sonja M. Koning, Sonja-Verena Albers, Wil N. Konings and Arnold J. M. Driessen ...
Red/ET Recombination
... freedom than any other technology and has become an essential component of the molecular biological tool kit. Recombineering can easily be deployed alongside conventional DNA methodologies to give you time to do your research. From its headquarters in Heidelberg, Gene Bridges provides recombineering ...
... freedom than any other technology and has become an essential component of the molecular biological tool kit. Recombineering can easily be deployed alongside conventional DNA methodologies to give you time to do your research. From its headquarters in Heidelberg, Gene Bridges provides recombineering ...