Identification of genes from flat oyster Ostrea
... quantitative real time PCR. Quantitative real time PCR is commonly used to study gene expression due to high sensitivity, specificity, reproducibility and broad dynamic range [3-5]. Level of gene expression is determined by quantifying the relative amount of target mRNA between different conditions, ...
... quantitative real time PCR. Quantitative real time PCR is commonly used to study gene expression due to high sensitivity, specificity, reproducibility and broad dynamic range [3-5]. Level of gene expression is determined by quantifying the relative amount of target mRNA between different conditions, ...
... binding sequence). Cell-free systems (eukaryotic [e.g., TNT® systems] and prokaryotic [e.g., S30 systems]) are fast, amenable to high-throughput, and often used for pull-down experiments and enzymatic assays (2,3); these systems require a bacteriophage promoter such as T7 or SP6. Promoter and vector ...
Changes in gene expression associated with acclimation to constant
... have been generated as a result of this study. The most daily temperature fluctuations. Control of cell growth and notable of these hypotheses is the possibility that the high proliferation appears to be an important part of the mobility group b1 (HMGB1) protein, which plays key response to change i ...
... have been generated as a result of this study. The most daily temperature fluctuations. Control of cell growth and notable of these hypotheses is the possibility that the high proliferation appears to be an important part of the mobility group b1 (HMGB1) protein, which plays key response to change i ...
here. - the DeRisi Lab
... of synthetic oligonucleotide probes eliminates the need for PCR. By carefully selecting probes from the unique regions, this platform provides a means to readily distinguish ...
... of synthetic oligonucleotide probes eliminates the need for PCR. By carefully selecting probes from the unique regions, this platform provides a means to readily distinguish ...
CRISPR/Cas9: Tools and Applications for Eukaryotic Genome Editing
... Knowing now that Cas9 works well, one of the immediate concerns in everyone’s mind was the specificity of the system. Figure 12 shows that Cas9 does have off-target activities and these tend to occur when there are mismatches between the guide RNA and the target DNA on the PAM-distal side of Cas9. ...
... Knowing now that Cas9 works well, one of the immediate concerns in everyone’s mind was the specificity of the system. Figure 12 shows that Cas9 does have off-target activities and these tend to occur when there are mismatches between the guide RNA and the target DNA on the PAM-distal side of Cas9. ...
The common northern periwinkle, Littorina littorea
... 1990). The critical pO2 values that stimulate these transitions differ between species and contribute to the differential success of various species in hypoxic or polluted environments (de Zwaan et al., 1992). Metabolic rate depression greatly extends the time that a fixed reserve of internal fuels ...
... 1990). The critical pO2 values that stimulate these transitions differ between species and contribute to the differential success of various species in hypoxic or polluted environments (de Zwaan et al., 1992). Metabolic rate depression greatly extends the time that a fixed reserve of internal fuels ...
Jhe in Gryllus assimilis: Cloning, sequence
... To determine whether the JHE coding sequence differs between crickets selected for high or low hemolyph JHE activity, total RNA was isolated individually from four high activity and four low activity G. assimilis using Trizol (Invitrogen). RNA was treated with DNAase, converted into cDNA using 200u ...
... To determine whether the JHE coding sequence differs between crickets selected for high or low hemolyph JHE activity, total RNA was isolated individually from four high activity and four low activity G. assimilis using Trizol (Invitrogen). RNA was treated with DNAase, converted into cDNA using 200u ...
DNA Structure Changes Coupled to Protein Binding
... encoding proteins, however, DNA contains noncoding sequences that regulate the use and propagation of that information. These regulatory processes involve proteins that ensure the correct conversion and processing of the DNA information, thus providing timely and spaceintegrated execution of the dev ...
... encoding proteins, however, DNA contains noncoding sequences that regulate the use and propagation of that information. These regulatory processes involve proteins that ensure the correct conversion and processing of the DNA information, thus providing timely and spaceintegrated execution of the dev ...
Biological ontologies for human functional annotation and
... “Sources are biological locations where substances are found …” ...
... “Sources are biological locations where substances are found …” ...
A major root-associated acid phosphatase in
... cross using the T-DNA insertion lines of GK-151C09 (atpap12), SALK_061597 (atpap15), and SALK_152821 (atpap26). Analysis of root-associated APase activity For histochemical staining of APase activity on the root surface of Arabidopsis seedlings, an agar solution (0.5%, w/v) containing 0.01% (w/v) BC ...
... cross using the T-DNA insertion lines of GK-151C09 (atpap12), SALK_061597 (atpap15), and SALK_152821 (atpap26). Analysis of root-associated APase activity For histochemical staining of APase activity on the root surface of Arabidopsis seedlings, an agar solution (0.5%, w/v) containing 0.01% (w/v) BC ...
Expanded roles of leucine-responsive regulatory protein in
... novel target genes for utilization of amino acids for protein synthesis, including tRNAs, aminoacyl-tRNA synthases and rRNAs. Northern blot analysis indicated alteration of mRNA levels for at least some novel targets, including the aminoacyltRNA synthetase genes. Phenotype MicroArray of the lrp muta ...
... novel target genes for utilization of amino acids for protein synthesis, including tRNAs, aminoacyl-tRNA synthases and rRNAs. Northern blot analysis indicated alteration of mRNA levels for at least some novel targets, including the aminoacyltRNA synthetase genes. Phenotype MicroArray of the lrp muta ...
Signaling pathways in myocyte hypertrophy. Role of GATA4
... and β-myosin heavy chain (β-MHC), and by increasing expression and secretion of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP). Initially, the response is beneficial, but when prolonged, it leads to pathological cardiomyocyte hypertrophy. In this study, cardiomyocyte hypertrop ...
... and β-myosin heavy chain (β-MHC), and by increasing expression and secretion of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP). Initially, the response is beneficial, but when prolonged, it leads to pathological cardiomyocyte hypertrophy. In this study, cardiomyocyte hypertrop ...
Correlating mRNA and protein Abundance
... yeast cell favors the expression of shorter ORFs over longer ones (as opposed to long lightweight ORFs – see MW of aa) This selection is happening, for the most part at the transcriptome level -------------------------------------------------------------------------------------------------Neg Correl ...
... yeast cell favors the expression of shorter ORFs over longer ones (as opposed to long lightweight ORFs – see MW of aa) This selection is happening, for the most part at the transcriptome level -------------------------------------------------------------------------------------------------Neg Correl ...
Full contents - Scion Publishing
... Important Note from the Publisher The information contained within this book was obtained by Scion Publishing Ltd from sources believed by us to be reliable. However, while every effort has been made to ensure its accuracy, no responsibility for loss or injury whatsoever occasioned to any person act ...
... Important Note from the Publisher The information contained within this book was obtained by Scion Publishing Ltd from sources believed by us to be reliable. However, while every effort has been made to ensure its accuracy, no responsibility for loss or injury whatsoever occasioned to any person act ...
Transcriptional Regulation of the Human Toll
... transcriptional start sites of TLR2 mRNA were determined. The human TLR2 gene was found to consist of two 5ⴕ noncoding exons followed by a third coding exon. Alternative splicing of exon II was detected primarily in human blood monocytes. The proximal promoter, exon I, and part of intron I were foun ...
... transcriptional start sites of TLR2 mRNA were determined. The human TLR2 gene was found to consist of two 5ⴕ noncoding exons followed by a third coding exon. Alternative splicing of exon II was detected primarily in human blood monocytes. The proximal promoter, exon I, and part of intron I were foun ...
PDF - Biotechnology for Biofuels
... different pH conditions studied. The relative expression levels were presented as a fold change (log2) between the control sample (pH 7.3) and the test sample (pH 6.3). In total more than 290 genes were found to be differentially expressed, with 72% being up-regulated and only 28% down-regulated at ...
... different pH conditions studied. The relative expression levels were presented as a fold change (log2) between the control sample (pH 7.3) and the test sample (pH 6.3). In total more than 290 genes were found to be differentially expressed, with 72% being up-regulated and only 28% down-regulated at ...
access full article - Caister Academic Press
... become elucidated, more innovative efforts have been placed on producing these molecules in heterologous hosts such as E. coli, that are more amenable to physiological and genetic manipulations (Kealer et al., 1998; Gehring et al., 1997). However, a frequent problem with recombinant expression syste ...
... become elucidated, more innovative efforts have been placed on producing these molecules in heterologous hosts such as E. coli, that are more amenable to physiological and genetic manipulations (Kealer et al., 1998; Gehring et al., 1997). However, a frequent problem with recombinant expression syste ...
TNT SP6 High-Yield Wheat Germ Protein Expression System
... 5.B. Denaturing Gel Analysis of Radioactive-Labeled Translation Products A protocol for gel analysis of radiolabeled proteins is given below. For fluorescent detection of proteins using FluoroTect™ GreenLys tRNA, refer to the FluoroTect™ GreenLys in vitro Translation Labeling System Technical Bullet ...
... 5.B. Denaturing Gel Analysis of Radioactive-Labeled Translation Products A protocol for gel analysis of radiolabeled proteins is given below. For fluorescent detection of proteins using FluoroTect™ GreenLys tRNA, refer to the FluoroTect™ GreenLys in vitro Translation Labeling System Technical Bullet ...
Trends in Plant Science
... crystallography is that many proteins are made up of distinct structural units known as domains [7]. In most cases, these spatially defined domains are associated with assigned functions. Domains are usually defined by the presence and/or the combination of various protein secondary structures or sp ...
... crystallography is that many proteins are made up of distinct structural units known as domains [7]. In most cases, these spatially defined domains are associated with assigned functions. Domains are usually defined by the presence and/or the combination of various protein secondary structures or sp ...
Embryonic activation of the myoD gene is regulated
... although myogenin knockout mice show severe muscle differentiation defects, myoblasts are present in approximately normal numbers and positions in these embryos (Hasty et al., 1993; Nabeshima et al., 1993). These data indicate that myoD and myf5 serve upstream functions in the myogenic developmental ...
... although myogenin knockout mice show severe muscle differentiation defects, myoblasts are present in approximately normal numbers and positions in these embryos (Hasty et al., 1993; Nabeshima et al., 1993). These data indicate that myoD and myf5 serve upstream functions in the myogenic developmental ...
Normalization between a pair of arrays
... •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g diseased versus healthy tissues) However background correction and normalization are necessary before making useful decisions or conclusions ...
... •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g diseased versus healthy tissues) However background correction and normalization are necessary before making useful decisions or conclusions ...
Lecture 9
... •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g diseased versus healthy tissues) However background correction and normalization are necessary before making useful decisions or conclusions ...
... •Microarray technology can be used to learn which genes are expressed differently in a target sample compared to a control sample (e.g diseased versus healthy tissues) However background correction and normalization are necessary before making useful decisions or conclusions ...
Identification and characterisation of SMIM1
... one studying the disease multiple myeloma. Three studies have been published in Nature Genetics, Scientific Reports and Blood and one is an almost submissionready manuscript. The Vel blood group antigen is present on red blood cells from all humans except rare Vel-negative individuals who can form a ...
... one studying the disease multiple myeloma. Three studies have been published in Nature Genetics, Scientific Reports and Blood and one is an almost submissionready manuscript. The Vel blood group antigen is present on red blood cells from all humans except rare Vel-negative individuals who can form a ...