novel 4E-interacting protein in Leishmania is involved in stage
... at the 50 -end of mRNAs. eIF4F consists of a cap-binding protein, eIF4E; a large scaffold MIF4G-domain protein, eIF4G; and a DEAD-box RNA helicase, eIF4A, which promotes RNA secondary structure unfolding in the 50 -UTR for scanning by the complex until the first AUG codon is reached. In higher eukary ...
... at the 50 -end of mRNAs. eIF4F consists of a cap-binding protein, eIF4E; a large scaffold MIF4G-domain protein, eIF4G; and a DEAD-box RNA helicase, eIF4A, which promotes RNA secondary structure unfolding in the 50 -UTR for scanning by the complex until the first AUG codon is reached. In higher eukary ...
Molecular cloning, characterization, and homologous
... Chitin, a polysaccharide composed of β-1,4-linked N-acetyl-D-glucosamine (GlcNAc), is highly distributed in nature as a major structural component of the exoskeleton of insects and crustaceans (1). It is ...
... Chitin, a polysaccharide composed of β-1,4-linked N-acetyl-D-glucosamine (GlcNAc), is highly distributed in nature as a major structural component of the exoskeleton of insects and crustaceans (1). It is ...
Gene Section BRCA1 (breast cancer 1, early onset) in Oncology and Haematology
... many of which are rare. These mutations are distributed uniformly along the entire coding region and intronic sequences flanking each exon. The mutations are at a high penetrance therefore women who carry these mutations have a lifetime risk of 80-90% to develop breast cancer. Founder mutations such ...
... many of which are rare. These mutations are distributed uniformly along the entire coding region and intronic sequences flanking each exon. The mutations are at a high penetrance therefore women who carry these mutations have a lifetime risk of 80-90% to develop breast cancer. Founder mutations such ...
Lec 19
... Marker genes Marker genes and reporter genes are utilized for selection and identification of the clones. These use phenotypic markers, identification from a gene library and DNA sequencing. DNA sequencing helps in determining the precise order of nucleotides in a piece of DNA. Construction and Iden ...
... Marker genes Marker genes and reporter genes are utilized for selection and identification of the clones. These use phenotypic markers, identification from a gene library and DNA sequencing. DNA sequencing helps in determining the precise order of nucleotides in a piece of DNA. Construction and Iden ...
Chapter 20
... • One method of introducing recombinant DNA into eukaryotic cells is electroporation, applying a brief electrical pulse to create temporary holes in plasma membranes • Alternatively, scientists can inject DNA into cells using microscopically thin needles • Once inside the cell, the DNA is incorpora ...
... • One method of introducing recombinant DNA into eukaryotic cells is electroporation, applying a brief electrical pulse to create temporary holes in plasma membranes • Alternatively, scientists can inject DNA into cells using microscopically thin needles • Once inside the cell, the DNA is incorpora ...
... amino acid and nudeotide sequence homology to a human eDNA (Kaumeyer, J.F., Polazzi, J.O. and Kotiek, M.P. (1986) Nucleic Acids Res. 14, 7839-7850), the 5' amino terminus was found to code for at-miernglebulin (at-M), a 183 amino acid protein belonging to the lipocalin protein superfamily (Pervaiz, ...
The polymerase chain reaction
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
Intracellular Distribution of Radioactivity in Nucleic Acid tration of
... ous structural and functional elements of the cell. For this purpose, @32and glycine-@-C'4 were chosen. It is now well established that nibonucleic acid (RNA) behaves in an intracellulanly heterogeneous fashion. In 1948 Marshak (36) reported that con siderably more @32 was incorporated into the RNA ...
... ous structural and functional elements of the cell. For this purpose, @32and glycine-@-C'4 were chosen. It is now well established that nibonucleic acid (RNA) behaves in an intracellulanly heterogeneous fashion. In 1948 Marshak (36) reported that con siderably more @32 was incorporated into the RNA ...
Cancer Prone Disease Section Pallister Hall syndrome (PHS) in Oncology and Haematology
... Mutations Germinal: Over 36 mutations have been identified in individuals with PHS. All mutations identified to date predict a truncated protein. Mutations that cause PHS are thought to result in the production of a constituitive repressor protein. The majority of truncating mutations in the middle ...
... Mutations Germinal: Over 36 mutations have been identified in individuals with PHS. All mutations identified to date predict a truncated protein. Mutations that cause PHS are thought to result in the production of a constituitive repressor protein. The majority of truncating mutations in the middle ...
SURVEY AND SUMMARY Origins of tmRNA: the
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
Sequence±structure±function studies of tRNA
... diverse class of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases. The enzymes characterized to date include members of two unrelated superfamilies: `classical' Rossmann-fold-like (1,2) and SPOUT (3). The relatively small SPOUT superfamily includes only a few characterized RNAspeci®c en ...
... diverse class of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases. The enzymes characterized to date include members of two unrelated superfamilies: `classical' Rossmann-fold-like (1,2) and SPOUT (3). The relatively small SPOUT superfamily includes only a few characterized RNAspeci®c en ...
Structure and expression of the PHO80 gene of Saccharomyces
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
Handout 14, 15 - U of L Class Index
... Proteins equivalent to chaperons and chaperonins have been identified. Eukaryotic folding makes less use of chaperonins, and more depends upon the action of Hsp70 chaperons. ...
... Proteins equivalent to chaperons and chaperonins have been identified. Eukaryotic folding makes less use of chaperonins, and more depends upon the action of Hsp70 chaperons. ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Anabaena - Oxford Academic
... exchange mechanism. In the case of Anahaena sp. PCC 7120, the ral gene, encoding transaldolase, lies between ;M?f and &J. ...
... exchange mechanism. In the case of Anahaena sp. PCC 7120, the ral gene, encoding transaldolase, lies between ;M?f and &J. ...
Green Fluorescent Protein: A Reporter Molecule
... Study of biological processes (example: synthesis of proteins) ...
... Study of biological processes (example: synthesis of proteins) ...
TNT SP6 High-Yield Wheat Germ Protein Expression
... prepared from an optimized wheat germ extract and contains all the components (tRNA, ribosomes, amino acids, polymerase, and translation initiation, elongation and termination factors) necessary for protein synthesis directly from DNA templates. The TnT® SP6 High-Yield Wheat Germ Protein Expression ...
... prepared from an optimized wheat germ extract and contains all the components (tRNA, ribosomes, amino acids, polymerase, and translation initiation, elongation and termination factors) necessary for protein synthesis directly from DNA templates. The TnT® SP6 High-Yield Wheat Germ Protein Expression ...
TbMP42 is a structure-sensitive ribonuclease that likely follows a
... reaction platform for the individual steps of the processing cycle (1–3). An editing cycle starts with the annealing of a pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mR ...
... reaction platform for the individual steps of the processing cycle (1–3). An editing cycle starts with the annealing of a pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mR ...
Putrescine oxidase of Micrococcus rubens : primary
... ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transformants were selected on 2 x YT medium containing 50 pg ampicillin ml-'. Colony hybridization (Grunstein & Hogness, 1975) with the 32P-labelledprobe designed for the NH,-terminal amino acid seque ...
... ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transformants were selected on 2 x YT medium containing 50 pg ampicillin ml-'. Colony hybridization (Grunstein & Hogness, 1975) with the 32P-labelledprobe designed for the NH,-terminal amino acid seque ...
ATP citrate lyase – biology and implication in human
... and cholesterol. Since its discovery half a century ago, many of the mechanisms involved in the regulation of ACLY activity have remained unclear. Various attempts to biochemically characterize the enzyme gave highly heterogeneous results. The development of expression systems for the recombinant hu ...
... and cholesterol. Since its discovery half a century ago, many of the mechanisms involved in the regulation of ACLY activity have remained unclear. Various attempts to biochemically characterize the enzyme gave highly heterogeneous results. The development of expression systems for the recombinant hu ...
Defective HIV-1 Proviruses Can Be Transcribed Upon Activation
... Methods. To understand how T cell activation affects the transcription of HIV-1 proviruses, resting CD4+ T cells from aviremic patients under suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine w ...
... Methods. To understand how T cell activation affects the transcription of HIV-1 proviruses, resting CD4+ T cells from aviremic patients under suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine w ...
The Cytoskeleton
... GTPases: cdc42: its activation triggers actin polymerization and bundling at ...
... GTPases: cdc42: its activation triggers actin polymerization and bundling at ...
Supplementary document Trehalose/2
... the cell-free, protein-free, extract was used for quantification of intracellular solutes and ...
... the cell-free, protein-free, extract was used for quantification of intracellular solutes and ...
PDF
... Genes for the primary replication machinery, the DNA polydifferent transposases flanking a hypothetical protein. The merase III complex in T. tengcongensis, are similar to those of most abundant one, a 1596-bp repeat (TLR008), consisting of well-characterized components in Escherichia coli, which is ...
... Genes for the primary replication machinery, the DNA polydifferent transposases flanking a hypothetical protein. The merase III complex in T. tengcongensis, are similar to those of most abundant one, a 1596-bp repeat (TLR008), consisting of well-characterized components in Escherichia coli, which is ...
PDF only - at www.arxiv.org.
... [2]. Some strains of haloarchaea have bacteriorhodopsin bilayer membrane components that serve as light-driven proton pumps. Light energy is used to pump protons out of the cell and thereby generate an electrochemical potential. This, in turn drives ATP synthesis. This is similar in nature to the me ...
... [2]. Some strains of haloarchaea have bacteriorhodopsin bilayer membrane components that serve as light-driven proton pumps. Light energy is used to pump protons out of the cell and thereby generate an electrochemical potential. This, in turn drives ATP synthesis. This is similar in nature to the me ...