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A differential response element for the homeotics at the Antennapedia P1 promoter of Drosophila. Proc. Natl. Acad. Sci. USA 91, 7420-7424 .pdf
A differential response element for the homeotics at the Antennapedia P1 promoter of Drosophila. Proc. Natl. Acad. Sci. USA 91, 7420-7424 .pdf

... The distinct developmental functions of the homeoproteins presumably result from differences in target gene regulation: either they regulate different target genes or they regulate the same target genes differently. Such regulatory differences could be due to use of different regulatory elements (ta ...
TEACHER Mr - Woodland Hills School District
TEACHER Mr - Woodland Hills School District

... Explain how different genes are used to express different traits on cows, monkeys, cats or sharks. ...
How Scientists Think
How Scientists Think

... reappear, segregating in the F2 progeny as Mendel had predicted? In the F2, there were 3470 red-eyed flies and 782 white-eyed flies, roughly a 3:1 ratio. Allowing for some deficiency in recessives, this was not unlike what Mendel’s theory predicted. But in this first experiment, there was a result t ...
02 Cholesterol Metabolism2012-03-18 01:50617 KB
02 Cholesterol Metabolism2012-03-18 01:50617 KB

... • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage-activating protein (SCAP) is an intracellular cholesterol sensor ...
Nucleic Acids Research
Nucleic Acids Research

... nucleotide sequence of pCCI and pCC9 were identical. Further restriction enzyme analysis of Charon 4A-CC1 and Charon 4A-CC9 revealed that these 2 recombinant phage contain overlapping fragments of the same chicken cytochrome c gene. Restriction enzyme analysis of Ch4A-CCl, for instance, showed that ...
Enhancement of Nuclear Factor- B Acetylation by Coactivator p300
Enhancement of Nuclear Factor- B Acetylation by Coactivator p300

... tightly regulated by interaction with inhibitory I␬B proteins. The best studied Rel-I␬B interaction is that of I␬B ␣ with the NF-␬B p50-RelA dimer, and this interaction blocks the ability of NF-␬B to enter the nucleus and bind to DNA (4). Thus, in most cells, NF-␬B is present as a latent, inactive, ...
Abiogenesis
Abiogenesis

... • RNA is (probably) still too complex to have arisen in one step • Plausible precursors are required • This is an area of ongoing research ...
2 C
2 C

... Low insulin levels cause the hypothalamus to feel hungry You would seek out food to increase blood glucose levels The liver would enlarge as it stores more and more glycogen ...
handout
handout

... 2. The bond attaching the amino acid chain to the tRNA in the P site is broken and a peptide bond is formed to the new tRNA amino acid in the A site moving the growing chain from the P site to the A site. 3. The large subunit of the ribosome shifts one codon, moving the tRNA with the growing amino ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
A Study of the Asp110–Glu112 Region of EcoRII Restriction

... “Pro111” codons of the ecoRII gene was performed using the pR224H plasmid. To facilitate initial selection of mutants, site-directed mutagenesis of the “Glu112” codon of the ecoRII gene was performed using the previously constructed mutant plasmid pR224HB (“Pro111” → “Ala111”), which had no BamHI re ...
Biochem10 - Amit Kessel Ph.D
Biochem10 - Amit Kessel Ph.D

... A. digest DNA duplex molecules from the 5'-OH ends. B. attack only single stranded DNA. C. have base sequence specificity for palindromes. D. digest double stranded DNA molecules randomly. E. can only act on DNA-RNA double helices. 46. Codons on mRNA may be accurately described by all of the followi ...
Isolation, Cloning, and Sequencing of the Salmonella typhimurium dd1A Gene with Purification and Characterization of its Product, D-Alanine:D-Alanine Ligase (ADP Forming).
Isolation, Cloning, and Sequencing of the Salmonella typhimurium dd1A Gene with Purification and Characterization of its Product, D-Alanine:D-Alanine Ligase (ADP Forming).

... Figure 1). The temperature-sensitive mutation in ST640, however, was never backcrossed into a nonmutagenized background. Thus, it has not been shown whether or not ddl is an essential structural gene for cell wall synthesis and cell viability in E. coli. The goal of this work, therefore, was 2-fold: ...


... FIG.1. Pathways for the incorporation of exogenous fatty ac- methano1:acetic acid (3:6:1, v/v) to remove unreacted [3H]palmitic acid ids into phospholipids. Exogenous fatty acids transit theouter membrane via interaction with the FadL protein. The fatty acids then d i f f i e and to precipitate [3Hl ...
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA

... subtilis by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin-kieselguhr (MAK). The intrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of ...
TRANSLATION: How to make proteins?
TRANSLATION: How to make proteins?

... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
Cloning and characterization of the Xenopus laevis p8 gene
Cloning and characterization of the Xenopus laevis p8 gene

... (Fig. 2c) and, given that Xp8 is localized to the nucleus, it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-co ...
Primary sequence analysis of Xac catalases
Primary sequence analysis of Xac catalases

... identity) [6], which have not been characterized so far (Figure S3). Amino acid sequence ...
Comparison of modeling options for the mRNA Life cycle
Comparison of modeling options for the mRNA Life cycle

... transcription process, which produces copies of the genetic information in the form of mRNA molecules. The mRNA molecules are then transcribed into proteins, which are the machines that carry out all the functions in a living organism. The level of the single proteins is what determines the phenotyp ...
Fastest, Easiest Adenoviral System Ever
Fastest, Easiest Adenoviral System Ever

... (Figure 3, Panel B), with only a small loss in cloning efficiency (Table II). A ...
Appendix – Biology for Bioinformatics Fig A1.1 A typical
Appendix – Biology for Bioinformatics Fig A1.1 A typical

... Fig A1.25 Simplified presentation for the chain elongation. The vertical line represents the pentose and the slanting line denotes the phosphodiester bond. Bases are designated as N1, N2, etc. ...
ARF-Aux/IAA interactions through domain III/IV are not strictly
ARF-Aux/IAA interactions through domain III/IV are not strictly

... Figure 4. Dose response for 1-NAA on the expression of an integrated 2XD0:GUS auxinof an active chromatin state through histone responsive reporter gene in Arabidopsis nph4-1/arf7 protoplasts transfected with full-length acetylation and methylation/demethylation) or CTD truncated ARF5 or the ARF5 DB ...
Caenorhabditis elegans unc-60 gene encodes
Caenorhabditis elegans unc-60 gene encodes

... Two unc-60 transcripts, 1.3 and 0.7 kb in size, were detected. The transcripts share a single exon encoding only the initial methionine, yet encode proteins with homologous sequences. The predicted protein products are 165 and 152 amino acids in length and their sequences are 38% identical. Both pro ...
The identification of transcription factors expressed in the notochord
The identification of transcription factors expressed in the notochord

... The notochord is the distinctive characteristic of chordates; however, the knowledge of the complement of transcription factors governing the development of this structure is still incomplete. Here we present the expression patterns of seven transcription factor genes detected in the notochord of th ...
Amiito acid sequence of the testosterone
Amiito acid sequence of the testosterone

... of the protein by introducing bends and distorting helical regions. Sequences that have a high probabiiiity of forming alpha-helix and beta-sheet structures (23) are indicated by shaded bars and open bars, respectively, in Figure 3. The regions which could form either structure are indicated by disc ...
Molecular Cloning, Sequencing, and Expression of the Glutamine
Molecular Cloning, Sequencing, and Expression of the Glutamine

... had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits the use of G or C in the third position in codons for all 20 amino acids and in the first position for Arg and Leu; no choice exists in the secon ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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