Journal of Bacteriology
... ropA encodes a 36-kDa outer membrane protein of Rhizobium leguminosarum bv. viciae strain 248 which constitutes the low-Mr part of antigen group III (R. A. de Maagd, I. H. M. Mulders, H. C. J. Canter Cremers, and B. J. J. Lugtenberg, J. Bacteriol. 174:214–221, 1992). We observed that genes homologou ...
... ropA encodes a 36-kDa outer membrane protein of Rhizobium leguminosarum bv. viciae strain 248 which constitutes the low-Mr part of antigen group III (R. A. de Maagd, I. H. M. Mulders, H. C. J. Canter Cremers, and B. J. J. Lugtenberg, J. Bacteriol. 174:214–221, 1992). We observed that genes homologou ...
document
... excision determinants and plasmid transfer genes. INTEGRATE EXCISE -TRANSFER ON PLASMID (not covered in detail here). ...
... excision determinants and plasmid transfer genes. INTEGRATE EXCISE -TRANSFER ON PLASMID (not covered in detail here). ...
Alu Background Information
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
A1114-CFS-SD1 - Food Standards Australia New Zealand
... Mature corn (Zea mays) plants contain both female and male flowers and usually reproduce sexually by wind-pollination. This provides for both self-pollination and natural out-crossing between plants, both of which are undesirable since the random nature of the crossing leads to lower yields (CFIA 19 ...
... Mature corn (Zea mays) plants contain both female and male flowers and usually reproduce sexually by wind-pollination. This provides for both self-pollination and natural out-crossing between plants, both of which are undesirable since the random nature of the crossing leads to lower yields (CFIA 19 ...
AUG
... Ribosome - site of protein synthesis • ribosome provides the environment for controlling the interaction between mRNA and aminoacyl-tRNA ...
... Ribosome - site of protein synthesis • ribosome provides the environment for controlling the interaction between mRNA and aminoacyl-tRNA ...
Expression Profiling of Fixed and Unfixed Tissue - Sigma
... OmniPlex library synthesis followed by library amplification. To make the library, isolated RNA from fixed or unfixed tissue is first annealed to a nonself complementary primer comprised of a quasi random 3' end and a universal 5' end. The primer is extended by polymerase, displacing a single strand ...
... OmniPlex library synthesis followed by library amplification. To make the library, isolated RNA from fixed or unfixed tissue is first annealed to a nonself complementary primer comprised of a quasi random 3' end and a universal 5' end. The primer is extended by polymerase, displacing a single strand ...
Highly Efficient and Specific Multiplex PCR usingTaKaRa
... TaKaRa Taq DNA Polymerase Hot Start Version contains a mixture of highpurity TaKaRa Taq DNA Polymerase and a monoclonal antibody to Taq DNA Polymerase, which binds to the polymerase until the temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming ...
... TaKaRa Taq DNA Polymerase Hot Start Version contains a mixture of highpurity TaKaRa Taq DNA Polymerase and a monoclonal antibody to Taq DNA Polymerase, which binds to the polymerase until the temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming ...
Forever 100bp Ladder Personalizer
... Our unique 100 bp endless usage ladder system (patent pending) is clearly distinguished from any existing commercialized consumables 100 bp DNA ladder. This system supplies templates (plasmids) which will be used to amplify size markers. Since 12 differently sized fragments were cloned into plasmids ...
... Our unique 100 bp endless usage ladder system (patent pending) is clearly distinguished from any existing commercialized consumables 100 bp DNA ladder. This system supplies templates (plasmids) which will be used to amplify size markers. Since 12 differently sized fragments were cloned into plasmids ...
by plasmid
... In most plasmids, the genes for proteins required for replication are located very close to the ori sequences at which they act. The genes in the ori region often determine many other properties of the plasmid. Therefore any DNA molecule with the ori region of a particular plasmid will have most of ...
... In most plasmids, the genes for proteins required for replication are located very close to the ori sequences at which they act. The genes in the ori region often determine many other properties of the plasmid. Therefore any DNA molecule with the ori region of a particular plasmid will have most of ...
Dehydrogenase in Saccharomyces cerevisiae
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
Principles of transcriptional control in the metabolic
... the directionality of the associated reactions, such junctions could requirements, at both the transcriptional and the post-transcriptional integrate metabolic flow (convergent junction) or could allow the flow levels. to diverge in two directions (divergent junction; see junction classification in ...
... the directionality of the associated reactions, such junctions could requirements, at both the transcriptional and the post-transcriptional integrate metabolic flow (convergent junction) or could allow the flow levels. to diverge in two directions (divergent junction; see junction classification in ...
Dehydrogenase in Saccharomyces cerevisiae
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
... -571), which would be read in the opposite sense, is capable of encoding a polypeptide of 77 residues; the other (sequence not complete, terminating at - 858) could encode a polypeptide of at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predi ...
Article Parallel Histories of Horizontal Gene
... Identification of Preferentially Expressed Genes in the Psyllid Bacteriome by mRNA-Seq Deep strand-specific sequencing of polyadenylated transcripts (mRNA-seq) in P. venusta nymphs isolated from hackberry galls was used to broadly identify and characterize psyllid genes. Furthermore, by comparing ex ...
... Identification of Preferentially Expressed Genes in the Psyllid Bacteriome by mRNA-Seq Deep strand-specific sequencing of polyadenylated transcripts (mRNA-seq) in P. venusta nymphs isolated from hackberry galls was used to broadly identify and characterize psyllid genes. Furthermore, by comparing ex ...
EPIgeneousTM Binding Domain Assays
... of reader domain binding. Binding to the biotinylated peptide substrate, the GST-tagged reader domain protein is detected by a conjugate comprised of a donor Cryptate-labeled antibody raised against the donor GST tag, and a streptavidin-acceptor, to detect biotin-peptide. Reader domain interaction w ...
... of reader domain binding. Binding to the biotinylated peptide substrate, the GST-tagged reader domain protein is detected by a conjugate comprised of a donor Cryptate-labeled antibody raised against the donor GST tag, and a streptavidin-acceptor, to detect biotin-peptide. Reader domain interaction w ...
P4-0065 RNA/DNA/Protein Purification Kit
... Norgen’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus only the genomic DNA and RNA will bind to the column while the proteins are removed in the flowthrough. Next, the bound RNA is washed with the provided RNA Wash Solution to remove impurities, and the purified R ...
... Norgen’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus only the genomic DNA and RNA will bind to the column while the proteins are removed in the flowthrough. Next, the bound RNA is washed with the provided RNA Wash Solution to remove impurities, and the purified R ...
The Role of Histone Modifications in Transcriptional Regulation upon Stress
... transcription is performed by three different RNA polymerases (RNA Pol), each of them specialized in a subset of mRNA molecules. RNA Pol I is responsible for transcription of ribosomal RNA genes, RNA Pol II transcribes most of the protein-coding genes and RNA Pol III transcribes tRNA genes, 5S RNA g ...
... transcription is performed by three different RNA polymerases (RNA Pol), each of them specialized in a subset of mRNA molecules. RNA Pol I is responsible for transcription of ribosomal RNA genes, RNA Pol II transcribes most of the protein-coding genes and RNA Pol III transcribes tRNA genes, 5S RNA g ...
Human Influenza A virus (M1) genesig Standard Kit
... recommend the use of a one step qRT-PCR protocol. One step qRT-PCR combines the reverse transcription and real-time PCR reaction in a simple closed tube protocol. This saves significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because ...
... recommend the use of a one step qRT-PCR protocol. One step qRT-PCR combines the reverse transcription and real-time PCR reaction in a simple closed tube protocol. This saves significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because ...
exam2review_s09.cwk (WP)
... the fate of pyruvate changes. Rather than proceeding on to the transition step and TCA, pyruvate or a derivative of pyruvate acts as an electron acceptor, thus producing commercially useful products like ethanol. This allows for the regeneration of NAD+ and thus glycolysis can continue to occur and ...
... the fate of pyruvate changes. Rather than proceeding on to the transition step and TCA, pyruvate or a derivative of pyruvate acts as an electron acceptor, thus producing commercially useful products like ethanol. This allows for the regeneration of NAD+ and thus glycolysis can continue to occur and ...
Worksheet 2
... What chromosome is the amplicon located on? ____ Click on the number underneath the tagged chromosome. Zoom all the way out. How long is the chromosome? _____ Mbp (Mega bp) or _______________ cM Where about on the chromosome is the bronze locus located? _______________ Zoom step-wise into the chromo ...
... What chromosome is the amplicon located on? ____ Click on the number underneath the tagged chromosome. Zoom all the way out. How long is the chromosome? _____ Mbp (Mega bp) or _______________ cM Where about on the chromosome is the bronze locus located? _______________ Zoom step-wise into the chromo ...
Biotechnology: Principles, Applications, and Social Implications From Protein to Product
... General Definition The application of technology to improve a biological organism Detailed Definition The application of the technology to modify the biological function of an organism by adding genes from another organism NDSU ...
... General Definition The application of technology to improve a biological organism Detailed Definition The application of the technology to modify the biological function of an organism by adding genes from another organism NDSU ...
sequence analysis of the 5` coi gene region from dama
... Comparative studies of mitochondrial DNA among different organisms have revealed both a general conserved organization across metazoa, and the existance of significant differences between groups (Morlais & Severson, 2002). These differences allowed the use of the DNA sequence of some specific mitoch ...
... Comparative studies of mitochondrial DNA among different organisms have revealed both a general conserved organization across metazoa, and the existance of significant differences between groups (Morlais & Severson, 2002). These differences allowed the use of the DNA sequence of some specific mitoch ...
Molecular and Immunological Methods
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
Standard 5 Lesson Plans
... What is the function and difference of DNA, RNA and Proteins? DNA is a template to make RNA. DNA is a deoxyribose sugar and has thymine as one of its bases. RNA is a ribose sugar and has uracil as one of its bases. Ribonucleic acid or RNA is a nucleic acid polymer consisting of nucleotide monomers t ...
... What is the function and difference of DNA, RNA and Proteins? DNA is a template to make RNA. DNA is a deoxyribose sugar and has thymine as one of its bases. RNA is a ribose sugar and has uracil as one of its bases. Ribonucleic acid or RNA is a nucleic acid polymer consisting of nucleotide monomers t ...
Towards evolving a better repressor
... as specificity of the operator. In the absence of discrimination by the repressor for its target DNA sequence, the repressor protein would wreak havoc in the cell by interfering with alternate regulatory networks. In addition, plasticity in the recognition of repressors by the operator site would le ...
... as specificity of the operator. In the absence of discrimination by the repressor for its target DNA sequence, the repressor protein would wreak havoc in the cell by interfering with alternate regulatory networks. In addition, plasticity in the recognition of repressors by the operator site would le ...