DNA, RNA and Protein Synthesis 1. Define: Nucleotide
... require the DNA double-helix to unwind until a new primase binding site is made available. Primase builds a small RNA segment that can serve as a primer and then DNA polymerase can add nucleotides to it. Each Okazaki fragment contains a small section of RNA nucleotides that must be removed (by DNA p ...
... require the DNA double-helix to unwind until a new primase binding site is made available. Primase builds a small RNA segment that can serve as a primer and then DNA polymerase can add nucleotides to it. Each Okazaki fragment contains a small section of RNA nucleotides that must be removed (by DNA p ...
I. Biology (35 points total) The following questions cover some of the
... An eukaryotic gene starts with a promoter, where various transcription factors and RNA polymerase bind to initiate transcription. It’s usually upstream of the exon; Exon is the coding sequence of the gene; Some times, a gene has introns, which are intervening sequences between exons and are spiced o ...
... An eukaryotic gene starts with a promoter, where various transcription factors and RNA polymerase bind to initiate transcription. It’s usually upstream of the exon; Exon is the coding sequence of the gene; Some times, a gene has introns, which are intervening sequences between exons and are spiced o ...
Inquiry into Life Twelfth Edition
... to form the preinitiation complex • Activators and general transcription factors also interact • Activators usually interact with one another in activating a gene – Individual factors interact to form a protein dimer facilitating binding to a single DNA target site – Specific factors bound to differ ...
... to form the preinitiation complex • Activators and general transcription factors also interact • Activators usually interact with one another in activating a gene – Individual factors interact to form a protein dimer facilitating binding to a single DNA target site – Specific factors bound to differ ...
Topic 4 Genetics
... [Gene: a heritable factor that controls a specific characteristic. ( The difference between structural genes, regulator genes, and genes coding for tRNA and rRNA are not expected at SL] Humans have around 25,000 different genes. Note that another reason different ...
... [Gene: a heritable factor that controls a specific characteristic. ( The difference between structural genes, regulator genes, and genes coding for tRNA and rRNA are not expected at SL] Humans have around 25,000 different genes. Note that another reason different ...
DNA Transcription and Translation
... nucleus so that the cell can make a protein out of the information obtained from the DNA in the gene Every 3 nitrogen bases in the DNA that makes up a gene is called a codon, and codes for a specific amino acid DNA does not leave the nucleus so a carrier molecule called messanger RNA (mRNA) i ...
... nucleus so that the cell can make a protein out of the information obtained from the DNA in the gene Every 3 nitrogen bases in the DNA that makes up a gene is called a codon, and codes for a specific amino acid DNA does not leave the nucleus so a carrier molecule called messanger RNA (mRNA) i ...
key
... 8. First strand cDNA is synthesized from mRNA by means of: a. DNA polymerase b. RNA polymerase c. DNA ligase d. S1 nuclease e. None of the above 9. To date, the type of enzyme used in the PCR reaction is: a. DNA polymerase I b. Klenow fragment c. a heat-stable DNA polymerase d. DNA ligase e. topoiso ...
... 8. First strand cDNA is synthesized from mRNA by means of: a. DNA polymerase b. RNA polymerase c. DNA ligase d. S1 nuclease e. None of the above 9. To date, the type of enzyme used in the PCR reaction is: a. DNA polymerase I b. Klenow fragment c. a heat-stable DNA polymerase d. DNA ligase e. topoiso ...
Prok transcription
... α, β, and β’ subunits make up the core. This core enzyme is potentially capable of copying DNA from any source to RNA In order to initiate transcription with high efficiency on bacterial promoters, the enzyme must combine with another polypeptide unit, - a sigma factor the sigma factor increas ...
... α, β, and β’ subunits make up the core. This core enzyme is potentially capable of copying DNA from any source to RNA In order to initiate transcription with high efficiency on bacterial promoters, the enzyme must combine with another polypeptide unit, - a sigma factor the sigma factor increas ...
One Step Quantitative Real-Time PCR Protocol
... DNA polymerase, which reverse transcribes RNA to cDNA in the presence of Mn2+ ion and polymerizes DNA during the PCR amplification. High-temperature (60–70°C) reverse transcription with rTth DNA polymerase permits efficient cDNA synthesis from RNA templates that contain a complex secondary structure ...
... DNA polymerase, which reverse transcribes RNA to cDNA in the presence of Mn2+ ion and polymerizes DNA during the PCR amplification. High-temperature (60–70°C) reverse transcription with rTth DNA polymerase permits efficient cDNA synthesis from RNA templates that contain a complex secondary structure ...
genomebiology.com
... in live cells, and highlights the dynamic nature of transcriptional regulation. ...
... in live cells, and highlights the dynamic nature of transcriptional regulation. ...
EMBARGOED UNTIL 00:01 (BST) WEDNESDAY 10 JUNE 2015
... EMBARGOED UNTIL 00:01 (BST) WEDNESDAY 10 JUNE ...
... EMBARGOED UNTIL 00:01 (BST) WEDNESDAY 10 JUNE ...
Transcription, RNA Processing, and
... Nascent RNA strand synthesis (elongation) occurs only in the 5’ 3’ direction, with new nucleotides added to the 3’ end of the nascent strand Transcription is catalyzed by DNA-directed RNA polymerases ...
... Nascent RNA strand synthesis (elongation) occurs only in the 5’ 3’ direction, with new nucleotides added to the 3’ end of the nascent strand Transcription is catalyzed by DNA-directed RNA polymerases ...
The Organization and Control of Eukaryotic Genomes
... Gene Amplification, Loss, or Rearrangement Gene amplification, loss, or rearrangement ...
... Gene Amplification, Loss, or Rearrangement Gene amplification, loss, or rearrangement ...
Chapter 19
... DNA contain a desired gene. A radioactive DNA Hybird is made (a single strand of a portion of DNA that is the desired gene, or part of it) If it binds to a sample of denatured (untwisted and unwound) DNA then you know the gene is in that sample. (fig 20.4) ...
... DNA contain a desired gene. A radioactive DNA Hybird is made (a single strand of a portion of DNA that is the desired gene, or part of it) If it binds to a sample of denatured (untwisted and unwound) DNA then you know the gene is in that sample. (fig 20.4) ...
3.4: Transcription and Translation
... sugar is deoxyribose in DNA and ribose in RNA; DNA is double stranded and RNA is single stranded; DNA has a (double) helix; DNA has thymine while RNA has uracil; (require full names written out) both contain four nitrogenous bases / A, G, C, T for DNA and A, G, C, U for RNA; [4 max] ...
... sugar is deoxyribose in DNA and ribose in RNA; DNA is double stranded and RNA is single stranded; DNA has a (double) helix; DNA has thymine while RNA has uracil; (require full names written out) both contain four nitrogenous bases / A, G, C, T for DNA and A, G, C, U for RNA; [4 max] ...
teachers.oregon.k12.wi.us
... Genes in the DNA sequence encode information about proteins and how those ...
... Genes in the DNA sequence encode information about proteins and how those ...
... a highly dynamic process [2], which sets obvious experimental challenges to most biochemical methods. In this context, imaging techniques have emerged as important tools to study the rapid succession of events that constitute transcription in real time [3]. Watching single cells over time also discl ...
File - Ms. Poole`s Biology
... sequence of DNA molecules that can direct the synthesis of a molecule product. • Genes do not all code for a protein, but all do code for an RNA molecule. • Some of those RNAs are translated into ...
... sequence of DNA molecules that can direct the synthesis of a molecule product. • Genes do not all code for a protein, but all do code for an RNA molecule. • Some of those RNAs are translated into ...
2054, Chap. 12, page 1 I. Genes: Expression and Regulation A
... 3. regulon = collection of genes or operons controlled by the same regulatory protein a. operons usually associated with a single pathway or function b. e.g., heat-shock proteins, glycerol catabolism 4. modulon = operons controlled by their own regulators that are also under the control of a common ...
... 3. regulon = collection of genes or operons controlled by the same regulatory protein a. operons usually associated with a single pathway or function b. e.g., heat-shock proteins, glycerol catabolism 4. modulon = operons controlled by their own regulators that are also under the control of a common ...
Protein Synthesis - mvhs
... REVIEW: PROTEIN SYNTHESIS TERMS Protein Synthesis Transcription Translation DNA Amino acids RNA Polymerase Enzymes Protein Ribosome ...
... REVIEW: PROTEIN SYNTHESIS TERMS Protein Synthesis Transcription Translation DNA Amino acids RNA Polymerase Enzymes Protein Ribosome ...
Activation sites and enhancer proteins
... poly binding at promoter •Activation sites and enhancer proteins = also aid in RNA poly binding; 1000s of bp away ...
... poly binding at promoter •Activation sites and enhancer proteins = also aid in RNA poly binding; 1000s of bp away ...
Regulation
... Regulation at the level of gene structure (inversion, flagella phase variation, or movement of a section of DNA, degree of methylation of DNA, degree of supercoiling). Control at the level of transcriptional level (level of methylation, degree of supercoiling, new sigma subunits, negative/positive r ...
... Regulation at the level of gene structure (inversion, flagella phase variation, or movement of a section of DNA, degree of methylation of DNA, degree of supercoiling). Control at the level of transcriptional level (level of methylation, degree of supercoiling, new sigma subunits, negative/positive r ...
Gene Section CRTC1 (CREB regulated transcription coactivator 1) Atlas of Genetics and Cytogenetics
... MECT1-MAML2; in the fusion protein the first 171 aa including the basic domain of MAML2 are replaced by 42 aa of MECT1; there are no sequence similarities in the N-terminal domains of MAML2 and MECT1; the fusion protein activates transcription of the Notch target gene HES1 independently of both Notc ...
... MECT1-MAML2; in the fusion protein the first 171 aa including the basic domain of MAML2 are replaced by 42 aa of MECT1; there are no sequence similarities in the N-terminal domains of MAML2 and MECT1; the fusion protein activates transcription of the Notch target gene HES1 independently of both Notc ...
Document
... the result of different rate of synthesis OR different degree of completion, as some genes have “transcription attenuation” sites. Inhibition of new RNA synthesis with toxins (e.g. Actinomycin D) can be used to measure the stability (half-life) of a mature RNA species. Or drive transcription of spec ...
... the result of different rate of synthesis OR different degree of completion, as some genes have “transcription attenuation” sites. Inhibition of new RNA synthesis with toxins (e.g. Actinomycin D) can be used to measure the stability (half-life) of a mature RNA species. Or drive transcription of spec ...