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Gel electrophoresis - University of California, Santa Barbara
Gel electrophoresis - University of California, Santa Barbara

... translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
From DNA to Protein - Stevenson High School
From DNA to Protein - Stevenson High School

... How does the cell get the info to the workers? ...
Looking within human genome
Looking within human genome

... chromosomes during their evolution • Organisms that have many sets of chromosomes are Polyploid. • Polyploid organisms can have very large genomes. • Human have lots of repetitive sequences in their genomes which range from150 to 300 base pair called Alu • Alu occurs more than 1.1 million times in h ...
Ch. 13.4: DNA Applications
Ch. 13.4: DNA Applications

... 1. What does PCR (polymerase chain reaction) do? 2. How goes gel electrophoresis work? 3. What is a DNA fingerprint? Why are DNA fingerprints unique to each person? ...
Chapter 8
Chapter 8

... • Mutations in these sequences affect promoter function. • The σ subunit binds to both regions. ...
lecture-1-viruses-an-introduction
lecture-1-viruses-an-introduction

... such as some Salmonella species, are classified into strains on the basis of the spectrum of phages to which they are susceptible. • Sources of enzymes. A number of enzymes used in molecular biology are virus enzymes. • Pesticides. Some insect pests are controlled with baculoviruses and myxoma virus ...
Short title: create species named Phormidium phage Pf
Short title: create species named Phormidium phage Pf

... The Pf-WMP3 genome contains 43,249 bp with 234 bp direct terminal repeats (NC_009551). The overall genome organization and core genes of this phage are distantly related to the Autographivirinae. The GC content of Pf-WMP3 genome is 46.49%. Based on the results of BLAST searches and modified by visua ...
Name
Name

... A gene of interest is identified. The plasmid and gene of interest are both cut with the same restriction enzyme. The gene is then inserted into the bacteria and DNA ligase binds the two fragments together ...
Document
Document

... The genome of any organism contains all the information for making that organism. The information is encoded in various types of genes that are transcribed into 4 types of RNA: mRNA - Messenger RNA: Encodes amino acid sequence of a polypeptide tRNA - Transfer RNA: Brings amino acids to ribosomes du ...
The control of gene expression
The control of gene expression

... Positive control of a regulatory system occurs only if an activator molecule interacts directly with the genome to turn on transcription. C. An example of Positive gene regulation The lac operon is under dual regulation which includes negative control by repressor protein and positive control by ca ...
Adobe Acrobat Document
Adobe Acrobat Document

... represent DNA or RNA? …how can you tell? ______________________ ______________________ ______________________ ______________________ ...
12-3: RNA
12-3: RNA

... Organisms have evolved many ways to protect their DNA from changes. In spite of these mechanisms, however, changes in the ________ occasionally do occur Any change in DNA sequence is called a _____________________. Mutations can be caused by errors in replication, transcription, cell division, or by ...
Transcription and Translation notes We often talk about how DNA is
Transcription and Translation notes We often talk about how DNA is

... Transcription  and  Translation  notes   We  often  talk  about  how  DNA  is  the  blue  print  of  an  organism.  At  this  point,  we  know  that  DNA  is   found  in  the  nucleus  of  a  cell,  and  that  DNA  can  be  re ...
Reproduction
Reproduction

... Deoxyribonucleic acid (DNA) and bonucIeic acid (ANA) are two of the cell’s most Important molecules. These nucleic acids have a complex three-dimensional structure that enab les them to direct protein synthesis in the cell. • Study the structure of the DNA and RNA molecules shown below. Fill in the ...
Lecture 17 Functional Genetics III Basic Approaches
Lecture 17 Functional Genetics III Basic Approaches

... Functional genomics: Identify the function of each and every gene in the genome. Since the characterization of the function of a protein domain in one organism generally provides hint to its function in another organism, the first goal of functional genomics is to identify as many genes as possible ...
Regulation of Gene Expression
Regulation of Gene Expression

... Summary 28.3 Regulation of Gene expression in Eukaryotes  Hormones affect the regulation of gene expression in one of two ways. Steroid hormones interact directly with intracellular receptors that are DNA-binding regulatory proteins; binding of the hormone has either positive or negative effects o ...
cDNA Micoroarray Data Analysis
cDNA Micoroarray Data Analysis

... combine the medical ontologies with learned patterns on sentences to extract relationships among proteins. • Usability, Performance and Scalability: Determine if the system is usable by biologists, if it can be easily trained to extract new types of relationships and its recall and precision is at a ...
Operons: The Basic Concept
Operons: The Basic Concept

... the operon by inactivating the repressor. In this way, the enzymes for lactose utilization are induced. Figure 18.22b ...
Supplementary information
Supplementary information

... where g and d denote averages, and the summation runs over the number of cell lines. This procedure creates one data point for each of the 1066 node vectors on the GI50 map and provides a visual mean to identify correlated gene responses according to specific map regions. Each gene correlation map y ...
BIO208
BIO208

... 2. Is strain "a" in question #1 prototroph or an auxotroph? Strain "b"? (Assume the strains are wildtype for all genes but the one in question). 3. Describe 5 steps involved in the lytic life cycle of bacteriophages. 4. Fill in ...
Learning Objectives
Learning Objectives

... 3. Explain how RNA differs from DNA. 4. Briefly explain how information flows from gene to protein. 5. Distinguish between transcription and translation. 6. Compare where transcription and translation occur in bacteria and in eukaryotes. 7. Define “codon” and explain the relationship between the lin ...
Chapter 13
Chapter 13

... • MicroRNAs regulate gene expression by base pairing with complementary sequences in target mRNAs. • RNA interference triggers degradation or translation inhibition of mRNAs complementary to miRNA or siRNA. • dsRNA may cause silencing of host genes. ...
Chapter 11
Chapter 11

...  By altering the sequence, a ribozyme can be engineered to cleave any mRNA sequence. ...
View file - University of California San Diego
View file - University of California San Diego

... of early Earth such as hydrogen cyanide, glycine, methylamine, hydrogen sulfide, ammonia, and others. All reacted well under these conditions, and formed compounds that contained the functional groups of 13 amino acids that make up proteins. The results offer a potential explanation for the selectio ...
5 questions per round and 9 rounds with 10 team tourney
5 questions per round and 9 rounds with 10 team tourney

... 8. What enzyme lays down the first nucleotides in replication? (RNA primase/ part of the primosome) 9. Where does transcription take place? (nucleus) 10. Which end of a DNA molecule has a terminal hydroxyl group? (3’) 11. Given that there is 20% T in a sample of DNA, what % is each of the other nucl ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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