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Bio426Lecture19Mar8 - NAU jan.ucc.nau.edu web server
Bio426Lecture19Mar8 - NAU jan.ucc.nau.edu web server

... Reviewing the Calvin cycle and counting carbon (C) atoms associated with one carboxylation. 1. Carboxylation. 1 CO2 binds to 1 RuBP (5C) producing two molecules of 3-PGA (total of 6 C). 2. Reduction. The two 3-PGA (3 C each) are reduced to two glyceraldehyde 3 phosphate (G3P, 3 C each) using ATP an ...
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... Acetyl CoA condenses with a 4C compound called Oxalo acetic acid (OAA), forming a 6C citric acid. As the first compound formed during kreb’s cycle is citric acid, this cycle is also called ‘citric acid’ cycle. Orgnic acids containing three carboxylic acid groups (COOH) are also formed during this cy ...
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...  As NADPH is utilized in reductive synthetic pathways, the increasing concentration of NADP+ stimulates the Pentose Phosphate Pathway, to replenish NADPH. The rest of the pathway converts ribulose-5-P to the 5-C product ribose-5-P, or to 3-C glyceraldehyde-3-P & 6-C fructose-6-P. Additional enzymes ...
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... mitochondria (one of transport systems for NADH into mitochondria) • Cytochrome c oxidase – the complex mitochondrial enzyme in the electron transport chain (from cytochrome c to oxygen) • ATPases – (eg. Ca ATPase of muscle cells) • Carbamyl phosphate synthase – enzyme of urea cycle • Growth hormone ...
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... by an enzyme, luciferase, that contains two proteins, designated LuxA and LuxB (Fig. 1). LuxAB sequentially binds FMNH2, O2, and an aliphatic aldehyde (RCHO) that are converted to an aliphatic acid, FMN, and water. In turn, they are released from the enzyme with the concomitant production of light ( ...
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... dehydrogenation o f fatty acid acyl-CoAs and glutaryl-CoA. However, dehydrogenation o f (C6-C10) fatty acid acyl-CoA derivatives and glutaryl-CoA was normal when the dehydrogenases were measured in fibroblast homogenate with artificial electron acceptors. In vivo, these dehydrogenases transfer their ...
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... active site of which is called the ‘H-cluster’. In other words, the structure and atomic coordination of catalytically essential (Fe,Ni)S centres of (Fe,Ni)S proteins are not inventions of the biological world, rather they are mimics of minerals that are indisputably older and which themselves have ...
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... ATP, the principal energy currency of the cell, fuels most biosynthetic reactions in the cytoplasm by its hydrolysis into ADP and inorganic phosphate. Because resynthesis of ATP occurs in the mitochondrial matrix, ATP is exported into the cytoplasm while ADP is imported into the matrix. The exchange ...
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... At the final, 3rd step, carnitine acyl transferase II regenarates fatty acyl CoA in the matrix, the relased carnitine back to intermemb via same transporter. Linkage of CoA and fatty acyl CoA pools in the cytosol (Biosynthesis) and ...
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... scale of complexity of photosynthesis, contemporary cyanobacteria, resembles closely to higher plants (Mulkidjanian et al., 2006). The cyanobacterial oxygenic photosynthesis is a multiplex cumulative process performed by diverse pigmentprotein complex located in thylakoid membranes inside cyanobacte ...
Midterm #2 - UC Davis Plant Sciences
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... a) Xylitol, an artificial sweetener sold under the brand name Xylosweet and a supplement in chewing gum, has been proven to reduce cavities. Xylitol (see structure below) is a sugar alcohol, which is converted into xylulose-5-P (Xu5P) by two reactions. Use the following information: ADP is a product ...
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... the energy of absorbed visible light to make organic compounds. These organic compounds are the starting point for all other biosynthetic pathway. The products of photosynthesis provide not only the substrate material but also chemical energy for all subsequent biosynthesis. The light of appropriate ...
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... energy status of developing seeds was obtained in research aiming to enhance the nutritional quality of seeds by increasing Lys accumulation (Zhu and Galili, 2003). Lys is an essential amino acid that serves a vital role in human food and livestock feed, since humans and some livestock (such as chic ...
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Light-dependent reactions

In photosynthesis, the light-dependent reactions take place on the thylakoid membranes. The inside of the thylakoid membrane is called the lumen, and outside the thylakoid membrane is the stroma, where the light-independent reactions take place. The thylakoid membrane contains some integral membrane protein complexes that catalyze the light reactions. There are four major protein complexes in the thylakoid membrane: Photosystem II (PSII), Cytochrome b6f complex, Photosystem I (PSI), and ATP synthase. These four complexes work together to ultimately create the products ATP and NADPH.[.The two photosystems absorb light energy through pigments - primarily the chlorophylls, which are responsible for the green color of leaves. The light-dependent reactions begin in photosystem II. When a chlorophyll a molecule within the reaction center of PSII absorbs a photon, an electron in this molecule attains a higher energy level. Because this state of an electron is very unstable, the electron is transferred from one to another molecule creating a chain of redox reactions, called an electron transport chain (ETC). The electron flow goes from PSII to cytochrome b6f to PSI. In PSI, the electron gets the energy from another photon. The final electron acceptor is NADP. In oxygenic photosynthesis, the first electron donor is water, creating oxygen as a waste product. In anoxygenic photosynthesis various electron donors are used.Cytochrome b6f and ATP synthase work together to create ATP. This process is called photophosphorylation, which occurs in two different ways. In non-cyclic photophosphorylation, cytochrome b6f uses the energy of electrons from PSII to pump protons from the stroma to the lumen. The proton gradient across the thylakoid membrane creates a proton-motive force, used by ATP synthase to form ATP. In cyclic photophosphorylation, cytochrome b6f uses the energy of electrons from not only PSII but also PSI to create more ATP and to stop the production of NADPH. Cyclic phosphorylation is important to create ATP and maintain NADPH in the right proportion for the light-independent reactions.The net-reaction of all light-dependent reactions in oxygenic photosynthesis is:2H2O + 2NADP+ + 3ADP + 3Pi → O2 + 2NADPH + 3ATPThe two photosystems are protein complexes that absorb photons and are able to use this energy to create an electron transport chain. Photosystem I and II are very similar in structure and function. They use special proteins, called light-harvesting complexes, to absorb the photons with very high effectiveness. If a special pigment molecule in a photosynthetic reaction center absorbs a photon, an electron in this pigment attains the excited state and then is transferred to another molecule in the reaction center. This reaction, called photoinduced charge separation, is the start of the electron flow and is unique because it transforms light energy into chemical forms.
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