An improved protocol for pulsed-field gel electrophoresis typing of
... at 37 8C. The lysozyme was removed and the plugs were rinsed with sterile water. The plugs were incubated with .20 U ml 1 proteinase K solution (100 ìl of .600 U ml 1 proteinase K stock in 2.5 ml of proteinase K reaction buffer) at 50 8C for 24–96 h. The plugs were washed four times in 13 wash buffe ...
... at 37 8C. The lysozyme was removed and the plugs were rinsed with sterile water. The plugs were incubated with .20 U ml 1 proteinase K solution (100 ìl of .600 U ml 1 proteinase K stock in 2.5 ml of proteinase K reaction buffer) at 50 8C for 24–96 h. The plugs were washed four times in 13 wash buffe ...
Biotechnology
... replaced with the diploid nucleus of a body cell, a process termed nuclear transplantation. The ability of a body cell to successfully form a cone decreases with embryonic development and cell differentiation. ...
... replaced with the diploid nucleus of a body cell, a process termed nuclear transplantation. The ability of a body cell to successfully form a cone decreases with embryonic development and cell differentiation. ...
CH 11 Study Guide: DNA, RNA, and Proteins
... 4. List the three types of RNA and explain the function of each. mRNA: carries the DNA message from the nucleus to the cytoplasm (to the ribosome) rRNA: forms the ribosome tRNA: carries amino acids to the ribosome so that proteins can be made 5. Who discovered the structure of DNA? Watson & Crick 6. ...
... 4. List the three types of RNA and explain the function of each. mRNA: carries the DNA message from the nucleus to the cytoplasm (to the ribosome) rRNA: forms the ribosome tRNA: carries amino acids to the ribosome so that proteins can be made 5. Who discovered the structure of DNA? Watson & Crick 6. ...
A. Nucleic Acid = polymer of nucleotides 1. nucleotide = molecule
... run in opposite directions 2. each terminal phosphate group is attached to a 5 ´ carbon, while the terminal OH groups are attached to 3 ´ carbons. 3. as a result the complementary strand must run in the opposite direction ...
... run in opposite directions 2. each terminal phosphate group is attached to a 5 ´ carbon, while the terminal OH groups are attached to 3 ´ carbons. 3. as a result the complementary strand must run in the opposite direction ...
1 - contentextra
... DNA includes covalent and hydrogen bonds. The hydrogen bonds allow linkage of the two chains of DNA between a purine and a pyrimidine nitrogenous base. Covalent bonds occur everywhere else within the DNA molecule. The covalent bonds are much stronger than the hydrogen bonds. Because of the weak hydr ...
... DNA includes covalent and hydrogen bonds. The hydrogen bonds allow linkage of the two chains of DNA between a purine and a pyrimidine nitrogenous base. Covalent bonds occur everywhere else within the DNA molecule. The covalent bonds are much stronger than the hydrogen bonds. Because of the weak hydr ...
Characterisation of DNA by Agarose Gel Electrophoresis and
... of a sugar moiety (de-oxyribose), a phosphoric acid residue and a base. Amongst the bases one can differentiate between the purines (adenine and guanine) and the pyrimidines (thymine und cytosine). Deoxyribose and phosphate residue are linked alternatively by 3’-5’-phosphodiester bonds and play an i ...
... of a sugar moiety (de-oxyribose), a phosphoric acid residue and a base. Amongst the bases one can differentiate between the purines (adenine and guanine) and the pyrimidines (thymine und cytosine). Deoxyribose and phosphate residue are linked alternatively by 3’-5’-phosphodiester bonds and play an i ...
DNA and Protein Synthesis Concept Questions
... 11. Proofreading enzymes scan DNA to check for base pairing errors. Explain why these enzymes are important. 12. Describe the technique of DNA profiling. 13. As a research biologist, you know of a bacterium that produces an antifungal molecule that is quite effective against a certain crop plant fun ...
... 11. Proofreading enzymes scan DNA to check for base pairing errors. Explain why these enzymes are important. 12. Describe the technique of DNA profiling. 13. As a research biologist, you know of a bacterium that produces an antifungal molecule that is quite effective against a certain crop plant fun ...
Section 1.1 Name:
... Review of Old Information: Recall that the DNA is the hereditary information for all living things. In this molecule is the code for all of our traits. However, one important question remains… how do we get from the genetic code from DNA in the nucleus, to the production of our phenotypes (or what w ...
... Review of Old Information: Recall that the DNA is the hereditary information for all living things. In this molecule is the code for all of our traits. However, one important question remains… how do we get from the genetic code from DNA in the nucleus, to the production of our phenotypes (or what w ...
DNA Review Questions (answers) no applications
... 17. Why do certain strands of DNA move farther than others in a gel? Why do they move at all? They move because DNA has a negative charge and is attracted to the positive end of the gel. Smaller strands can move faster through the porous gel than larger ones can. 18. Outline how you could extract DN ...
... 17. Why do certain strands of DNA move farther than others in a gel? Why do they move at all? They move because DNA has a negative charge and is attracted to the positive end of the gel. Smaller strands can move faster through the porous gel than larger ones can. 18. Outline how you could extract DN ...
Lecture 4, Exam III Worksheet Answers
... 1. What is the purpose of telomeres? What type of cells are they most important within? What types of cells are they least important in? What enzyme creates telomeres and how? What is special about the enzyme that allows it to carry out its function? There is a portion of a cell’s complementary DNA ...
... 1. What is the purpose of telomeres? What type of cells are they most important within? What types of cells are they least important in? What enzyme creates telomeres and how? What is special about the enzyme that allows it to carry out its function? There is a portion of a cell’s complementary DNA ...
What is a DNA?
... 4- Precipitate DNA • Add an equal volume of isopropanol. • DNA will be precipitated by gentle swirling & observed as a white thread like strand. • Using a sterile spatula or loop transfer the DNA strand into a sterile micro centrifuge tube containing 1 mL of 75% ethanol. ...
... 4- Precipitate DNA • Add an equal volume of isopropanol. • DNA will be precipitated by gentle swirling & observed as a white thread like strand. • Using a sterile spatula or loop transfer the DNA strand into a sterile micro centrifuge tube containing 1 mL of 75% ethanol. ...
LNUC IV.A - UTK-EECS
... ¶1. The Polymerase Chain Reaction (PCR) is a method for exponentially amplifying the concentration of a selected DNA sequence in a sample. ¶2. Primers: The sequence to be replicated is identified by a known short sequence (about 20 bases) at its beginning (50 end). Short DNA strands called primers, ...
... ¶1. The Polymerase Chain Reaction (PCR) is a method for exponentially amplifying the concentration of a selected DNA sequence in a sample. ¶2. Primers: The sequence to be replicated is identified by a known short sequence (about 20 bases) at its beginning (50 end). Short DNA strands called primers, ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.