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PLASMID ISOLATIONS (MINIPREPS)
... second is to use ethidium bromide in cesium chloride gradients. Ethidium bromide can fit between the stacked bases in DNA, this is termed intercalation. As it does this it forces the bases apart, causing the DNA to unwind and lengthen. However, less of the dye can intercalate into supercoiled DNA th ...
... second is to use ethidium bromide in cesium chloride gradients. Ethidium bromide can fit between the stacked bases in DNA, this is termed intercalation. As it does this it forces the bases apart, causing the DNA to unwind and lengthen. However, less of the dye can intercalate into supercoiled DNA th ...
Manipulation DNA
... D. Scientists identify differences in DNA by measuring the length and number of fragments created by digestion with restriction enzymes. A technique called gel electrophoresis is used to separate fragments according to length. DNA fragments (cut with an appropriate restriction enzyme) are placed on ...
... D. Scientists identify differences in DNA by measuring the length and number of fragments created by digestion with restriction enzymes. A technique called gel electrophoresis is used to separate fragments according to length. DNA fragments (cut with an appropriate restriction enzyme) are placed on ...
mRNA
... DNA can originate from a variety of sources: genomic DNA - from organisms plasmid DNA - circular, cloned fragments amplified DNA - specific fragments from PCR Knowing the size of the DNA is beneficial in identifying the fragments – distance migrated is inversely proportional to the size of the molec ...
... DNA can originate from a variety of sources: genomic DNA - from organisms plasmid DNA - circular, cloned fragments amplified DNA - specific fragments from PCR Knowing the size of the DNA is beneficial in identifying the fragments – distance migrated is inversely proportional to the size of the molec ...
DNA and Its Proccesses
... • Create ONE strand of mRNA from a piece of DNA • Unzip strands • Add mRNA base pairs to one side • Base-pairing rules: ...
... • Create ONE strand of mRNA from a piece of DNA • Unzip strands • Add mRNA base pairs to one side • Base-pairing rules: ...
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... withdraw tissues and then replaced the treated cells. DNA of unaffected gene is extracted from donor cell. This fragment of DNA is replicated using Polymerase Chain Reaction (PCR). The target piece of DNA needs to be sequenced which will have a promoter region where copying of the gene will begin an ...
... withdraw tissues and then replaced the treated cells. DNA of unaffected gene is extracted from donor cell. This fragment of DNA is replicated using Polymerase Chain Reaction (PCR). The target piece of DNA needs to be sequenced which will have a promoter region where copying of the gene will begin an ...
Some Products Made Using Biotechnology
... • Bacteria can make human insulin or human growth hormone. • Bacteria can be engineered to “eat” oil spills. ...
... • Bacteria can make human insulin or human growth hormone. • Bacteria can be engineered to “eat” oil spills. ...
Exam 1 - Faculty Web Pages
... B. can always be distinguished from one another because of the simple band pattern of the PCR fingerprint. C. are similar in that they provide a limited amount of information about the nucleotide sequences examined. D. None of the above 5. Restriction enzymes A. Were discovered during study of bacte ...
... B. can always be distinguished from one another because of the simple band pattern of the PCR fingerprint. C. are similar in that they provide a limited amount of information about the nucleotide sequences examined. D. None of the above 5. Restriction enzymes A. Were discovered during study of bacte ...
DNA etcTest Rev 07
... 10. A section of DNA that codes for a protein is a(n) gene. 11. Chargaff’s rule says that for every 3 thymines in a section of DNA there are 3 adenines. 12. Franklin and Wilkins studied DNA by taking x-ray pictures of it. 13. DNA carries the genetic code. 14. The sequence of N-bases is the genetic c ...
... 10. A section of DNA that codes for a protein is a(n) gene. 11. Chargaff’s rule says that for every 3 thymines in a section of DNA there are 3 adenines. 12. Franklin and Wilkins studied DNA by taking x-ray pictures of it. 13. DNA carries the genetic code. 14. The sequence of N-bases is the genetic c ...
Guided Notes
... Restriction maps show the lengths of DNA fragments.∫ Gel electrophoresis is used to _______________________________________________________. A DNA sample is cut into fragments with restriction enzymes. Electrical current pulls DNA fragments through a gel. ____________________________________ ...
... Restriction maps show the lengths of DNA fragments.∫ Gel electrophoresis is used to _______________________________________________________. A DNA sample is cut into fragments with restriction enzymes. Electrical current pulls DNA fragments through a gel. ____________________________________ ...
9-1
... –Many people have the same number of repeats in a certain region of DNA. –The probability that two people share identical numbers of repeats in several locations is very small. ...
... –Many people have the same number of repeats in a certain region of DNA. –The probability that two people share identical numbers of repeats in several locations is very small. ...
Supporting information PCR amplification and DGGE analysis The
... 72 °C, and a final 6 min extension at 72 °C. These PCR amplifications were carried out in ...
... 72 °C, and a final 6 min extension at 72 °C. These PCR amplifications were carried out in ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.