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Course: Immunology Lecturer: Dr. Weam Saad Practical Lecture
... 3. A ring of precipitation around the Ag well will be formed after the Ag-Ab reaction. This ring represents the immunocomplex that formed at equivalence zoon. 4. The gel is prepared by mixing the antibodies with the agarose gel then poured into plate. After the gel become solid, different dilutions ...
... 3. A ring of precipitation around the Ag well will be formed after the Ag-Ab reaction. This ring represents the immunocomplex that formed at equivalence zoon. 4. The gel is prepared by mixing the antibodies with the agarose gel then poured into plate. After the gel become solid, different dilutions ...
Summer 2007
... DNA/RNA, Protein Synthesis and Mutations - REVIEW I. Understand all vocabulary. II. Understand Cell Reproduction III. Understand the scientific process involved in establishing DNA as the heredity molecule. IV. Describe the structure of DNA V. Understand the processes of replication, transcription a ...
... DNA/RNA, Protein Synthesis and Mutations - REVIEW I. Understand all vocabulary. II. Understand Cell Reproduction III. Understand the scientific process involved in establishing DNA as the heredity molecule. IV. Describe the structure of DNA V. Understand the processes of replication, transcription a ...
I. virAL CHROMOSOMES
... (1) The number of times the two strands cross each other (2) T = L in non-supercoiled molecules d) L = W + T (1) If T decreases, either L will decrease or W will increase (2) The strain of underwiding can be accommodated by negative supercoiling (a) Single stranded region increases with temperature ...
... (1) The number of times the two strands cross each other (2) T = L in non-supercoiled molecules d) L = W + T (1) If T decreases, either L will decrease or W will increase (2) The strain of underwiding can be accommodated by negative supercoiling (a) Single stranded region increases with temperature ...
Chapter Objectives: Chapter 20 Biotechnology
... such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products 16. Describe how gene manipulation has practical applications for agriculture 17. Describe ho plant genes can be manipulated using the Ti plasmid carried by Agrobacterium ...
... such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products 16. Describe how gene manipulation has practical applications for agriculture 17. Describe ho plant genes can be manipulated using the Ti plasmid carried by Agrobacterium ...
ANSWERS TO REVIEW QUESTIONS
... 6. One end of a strand of nucleotides has a phosphate group attached to the 5' carbon of deoxyribose. The other end has a hydroxyl group attached to the 3' carbon. Phosphodiester bonds (which bind the sugar-phosphate backbone) form between the 3' OH of the nucleotide chain and the 5' phosphate of an ...
... 6. One end of a strand of nucleotides has a phosphate group attached to the 5' carbon of deoxyribose. The other end has a hydroxyl group attached to the 3' carbon. Phosphodiester bonds (which bind the sugar-phosphate backbone) form between the 3' OH of the nucleotide chain and the 5' phosphate of an ...
CHAPTER 12
... 1. Explain how the many types of adult human cells are formed. 2. Explain how RNA is processed in eukaryotes before it leaves the nucleus. Explain how this processing can result in different proteins from the same gene. 3. Explain how homeotic genes help us understand animal evolution and developmen ...
... 1. Explain how the many types of adult human cells are formed. 2. Explain how RNA is processed in eukaryotes before it leaves the nucleus. Explain how this processing can result in different proteins from the same gene. 3. Explain how homeotic genes help us understand animal evolution and developmen ...
Chapter 20 – DNA Technology - Fort Thomas Independent Schools
... 8. In polymerase chain reaction technology, the two strands of DNA are separated by: a) gel electrophoresis b) treating them with restriction enzymes c) centrifugation d) exposing them to high pH e) heating them until they “melt” 9. ________ is a technique that can be used to separate DNA molecules ...
... 8. In polymerase chain reaction technology, the two strands of DNA are separated by: a) gel electrophoresis b) treating them with restriction enzymes c) centrifugation d) exposing them to high pH e) heating them until they “melt” 9. ________ is a technique that can be used to separate DNA molecules ...
Goal 3: Learner will develop an understanding of the continuity of
... c. What weak bonds hold the complementary bases together? ________________________ 8. If the strand of DNA above undergoes transcription, what will the sequence of the mRNA be? ...
... c. What weak bonds hold the complementary bases together? ________________________ 8. If the strand of DNA above undergoes transcription, what will the sequence of the mRNA be? ...
LabChip GX/GXII Automated Electrophoresis Systems
... reproducible data than gels. • Faster time to result – Quantitative sizing and concentration data are automatically reported as each sample is analyzed. ...
... reproducible data than gels. • Faster time to result – Quantitative sizing and concentration data are automatically reported as each sample is analyzed. ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.