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universitetet i oslo
... are known for most genes in sequenced genomes 9. Telomers are located at the ends of ribosomal RNA in centromers in the middle of chromosomes at the ends of chromosomes in nuclear DNA in mitochondrial DNA in prokaryotes in eukaryotes ...
... are known for most genes in sequenced genomes 9. Telomers are located at the ends of ribosomal RNA in centromers in the middle of chromosomes at the ends of chromosomes in nuclear DNA in mitochondrial DNA in prokaryotes in eukaryotes ...
DNA – the heredity material DNA - genetic material Discovering
... Weak hydrogen bonds between base pairs hold DNA strands together. Each chain in the helix is a complimentary mirror image of the other. – Double helix unzips and undergoes semi-conservative replication. Confirmed ...
... Weak hydrogen bonds between base pairs hold DNA strands together. Each chain in the helix is a complimentary mirror image of the other. – Double helix unzips and undergoes semi-conservative replication. Confirmed ...
Restriction Enzymes
... • A restriction enzyme requires a specific double stranded recognition sequence of nucleotides to cut DNA. • Recognition sites are usually 4 to 8 base pairs in length. • Cleavage occurs within or near the site. • Both DNA strands in the site have the same base sequence when read 5' to 3'. Such sequ ...
... • A restriction enzyme requires a specific double stranded recognition sequence of nucleotides to cut DNA. • Recognition sites are usually 4 to 8 base pairs in length. • Cleavage occurs within or near the site. • Both DNA strands in the site have the same base sequence when read 5' to 3'. Such sequ ...
Studying and Manipulating Genomes
... DNA Gel Electrophoresis Technique by which molecules of different sizes are separated and analyzed Separated by electric charge - DNA is negatively charged and will migrate towards positive electric field Rate of migration depends on size of molecule ...
... DNA Gel Electrophoresis Technique by which molecules of different sizes are separated and analyzed Separated by electric charge - DNA is negatively charged and will migrate towards positive electric field Rate of migration depends on size of molecule ...
Life on Mars
... check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “false-positive” results. ...
... check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “false-positive” results. ...
Section 2: Figures
... The separation and visualization of DNA bands were performed as mentioned above. The band intensity was measured using “quantity one” software. The super-coiled plasmid DNA due to its compact structure was corrected by multiplying the intensity with 1.42. The intensity of different forms of plasmid ...
... The separation and visualization of DNA bands were performed as mentioned above. The band intensity was measured using “quantity one” software. The super-coiled plasmid DNA due to its compact structure was corrected by multiplying the intensity with 1.42. The intensity of different forms of plasmid ...
Deoxyribonucleic acid sodium salt from human placenta (D7011
... for modern molecular biochemistry and has been consistent with subsequent discoveries of RNA and protein biosynthesis. DNA was described as a double helix of a chain of nucleotides. Each nucleotide consists of a central carbohydrate moiety, 2’-deoxyribose, attached to a phosphate group on the 5-posi ...
... for modern molecular biochemistry and has been consistent with subsequent discoveries of RNA and protein biosynthesis. DNA was described as a double helix of a chain of nucleotides. Each nucleotide consists of a central carbohydrate moiety, 2’-deoxyribose, attached to a phosphate group on the 5-posi ...
DNA Structure and Function Notes
... What will be the MOST LIKELY effect of this on the reaction? ...
... What will be the MOST LIKELY effect of this on the reaction? ...
Objectives 2
... 1) List the types and principal functions of the nucleic acids. Deoxyribonucleic acid (DNA) is found in the cell nucleus and in the mitochondria and functions to store genetic information used for the synthesis of proteins and enzymes. Ribonucleic acid (RNA) is found in the nucleus, in the cytosol, ...
... 1) List the types and principal functions of the nucleic acids. Deoxyribonucleic acid (DNA) is found in the cell nucleus and in the mitochondria and functions to store genetic information used for the synthesis of proteins and enzymes. Ribonucleic acid (RNA) is found in the nucleus, in the cytosol, ...
MCDB 1030 – Spring 2003
... Phospholipids have polar and non-polar regions (they are amphipathic). In water they form bilayers so that the tails can associate with each other and “get away” from the water. The heads face the water side because they are polar. 7. (3 points) What is the common feature in the following molecules: ...
... Phospholipids have polar and non-polar regions (they are amphipathic). In water they form bilayers so that the tails can associate with each other and “get away” from the water. The heads face the water side because they are polar. 7. (3 points) What is the common feature in the following molecules: ...
Biology
... 1. Describe the structure of a chromosome. 2. Contrast the terms diploid and haploid. What types of cells have each? 3. Describe what occurs in each step of the cell cycle.(Interphase, Mitosis, and Cytokinesis) 4. Describe what occurs in each phase of mitosis. 5. Contrast cytokinesis in plant and an ...
... 1. Describe the structure of a chromosome. 2. Contrast the terms diploid and haploid. What types of cells have each? 3. Describe what occurs in each step of the cell cycle.(Interphase, Mitosis, and Cytokinesis) 4. Describe what occurs in each phase of mitosis. 5. Contrast cytokinesis in plant and an ...
Agarose gel electrophoresis
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Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.