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Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... run, including at least one ladder per comb. Be sure to vortex the loading buffer prior to aliquoting. Space all of the aliquots out so that they will not cross-contaminate. 12. For each PCR reaction, mix 5 µl of PCR product with a 1 µl aliquot of loading buffer on the Parafilm, and load the entire ...
... run, including at least one ladder per comb. Be sure to vortex the loading buffer prior to aliquoting. Space all of the aliquots out so that they will not cross-contaminate. 12. For each PCR reaction, mix 5 µl of PCR product with a 1 µl aliquot of loading buffer on the Parafilm, and load the entire ...
Chapter 4 Notes
... • When DNA replicates (is copied) the base pairs will only pair up with the complementary base (A to T, C to G). • When DNA is being copied it is similar to the DNA being unzipped and rezipped up with a new set of bases. Again the bases matching with the correct complementary base. ...
... • When DNA replicates (is copied) the base pairs will only pair up with the complementary base (A to T, C to G). • When DNA is being copied it is similar to the DNA being unzipped and rezipped up with a new set of bases. Again the bases matching with the correct complementary base. ...
Restriction Enzymes
... DAN sequence between them may change. Thus if two DNA molecules differ in sequence, they likely have different lengths for the fragments produced following treatment with restriction enzymes. Typical digestions included a unit of enzyme per microgram of starting DNA, and one enzyme unit usually (dep ...
... DAN sequence between them may change. Thus if two DNA molecules differ in sequence, they likely have different lengths for the fragments produced following treatment with restriction enzymes. Typical digestions included a unit of enzyme per microgram of starting DNA, and one enzyme unit usually (dep ...
Genomic_DNA - McMaster Chemistry
... hazardous reagents. The protocol we present here works for the isolation of genomic DNA from both Gramnegative bacteria (Ref. 7) and, with very small adaptions, for a broad range of Grampositive prokaryotes (Table 1). The purification scheme is simple, reproducible, fast and safe, since hazardous so ...
... hazardous reagents. The protocol we present here works for the isolation of genomic DNA from both Gramnegative bacteria (Ref. 7) and, with very small adaptions, for a broad range of Grampositive prokaryotes (Table 1). The purification scheme is simple, reproducible, fast and safe, since hazardous so ...
8.1-8.2 TAKE DOWN NOTES AND SKETCH MOLECULES
... Hershey & Chase confirmed that DNA is the material that passes on traits. ...
... Hershey & Chase confirmed that DNA is the material that passes on traits. ...
1. Explain how a gene directs the synthesis of an mRNA molecule
... the words and phrases: base-pairing rule, complementary nucleotides, cytoplasm, DNA, gene, messenger RNA, nucleotide, nucleus, RNA polymerase, amino acid, anti-codon, codon, cytoplasm, DNA, mRNA, nucleotide, nucleus, ribosome, RNA polymerase, tRNA, transcription, and translation. ...
... the words and phrases: base-pairing rule, complementary nucleotides, cytoplasm, DNA, gene, messenger RNA, nucleotide, nucleus, RNA polymerase, amino acid, anti-codon, codon, cytoplasm, DNA, mRNA, nucleotide, nucleus, ribosome, RNA polymerase, tRNA, transcription, and translation. ...
Chapter 5
... Restriction enzymes • Proteins; • Restriction endonucleases, recognize specific DNA sequence and cleave at specific DNA sequence (scalpels or scissors); • Discovered by Werner Arber and Hamilton Smith and first used by Daniel Nathans; • Usually recognize palindromic sequences. A man a plan a ca ...
... Restriction enzymes • Proteins; • Restriction endonucleases, recognize specific DNA sequence and cleave at specific DNA sequence (scalpels or scissors); • Discovered by Werner Arber and Hamilton Smith and first used by Daniel Nathans; • Usually recognize palindromic sequences. A man a plan a ca ...
DNA Fill in the blank notes.
... 3. Two new strands of DNA are formed. Because there is always one strand of old DNA, the number of ________________ is decreased. ...
... 3. Two new strands of DNA are formed. Because there is always one strand of old DNA, the number of ________________ is decreased. ...
Prescott`s Microbiology, 9th Edition Chapter 17 – Recombinant DNA
... Exonucleases cleave from the ends. Table 17.2 Which of the above enzymes yield blunt ends? Which yield sticky ends with a 5’ overhang? What about a 3’ overhang? The enzymes with blunt products is AluI. Enzymes with sticky 5’ overhang products include all the other enzymes. There are no enzymes pictu ...
... Exonucleases cleave from the ends. Table 17.2 Which of the above enzymes yield blunt ends? Which yield sticky ends with a 5’ overhang? What about a 3’ overhang? The enzymes with blunt products is AluI. Enzymes with sticky 5’ overhang products include all the other enzymes. There are no enzymes pictu ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.