Control of Chromosome Pairing and Genome Evolution in Disomic
... telomere) are derived from 1Am. RSL104 has a CS centromere and both telomeres are from CS, but has two interstitial segments derive from 1Am. See Figure 1 from Luo et al. (1996). Each of these lines was crossed to CS with Ph1 to form a mapping population (in which case only recombination within the ...
... telomere) are derived from 1Am. RSL104 has a CS centromere and both telomeres are from CS, but has two interstitial segments derive from 1Am. See Figure 1 from Luo et al. (1996). Each of these lines was crossed to CS with Ph1 to form a mapping population (in which case only recombination within the ...
08.seg_dup_els - NYU Computer Science
... each of such genome-evolution process occurs in history-independent manner and can be encoded faithfully using a Markov process. After the passage of sufficiently long time and assuming stationarity in evolutionary rates, the repeat configurations in the flanking regions reach a stationary distribut ...
... each of such genome-evolution process occurs in history-independent manner and can be encoded faithfully using a Markov process. After the passage of sufficiently long time and assuming stationarity in evolutionary rates, the repeat configurations in the flanking regions reach a stationary distribut ...
14 PCA and K-Means Decipher Genome
... distributions from the first case (a). The third case (c) produces only one distribution, which is symmetrical with respect to the ‘shifts’ (or rotations) in the first two cases, and there is a hypothesis that this is a result of genomic sequence evolution. This can be explained as follows. Vitality o ...
... distributions from the first case (a). The third case (c) produces only one distribution, which is symmetrical with respect to the ‘shifts’ (or rotations) in the first two cases, and there is a hypothesis that this is a result of genomic sequence evolution. This can be explained as follows. Vitality o ...
computation for chIP-seq and rNA-seq studies
... After these ‘regions’ are identified, processFigure 2 | ChIP-seq peak types from various experiments. (a–c) Data shown are from remapping of a ing ensues to identify the most likely source previously published human ChIP-seq dataset7. Proteins that bind DNA in a site-specific fashion, such point(s) ...
... After these ‘regions’ are identified, processFigure 2 | ChIP-seq peak types from various experiments. (a–c) Data shown are from remapping of a ing ensues to identify the most likely source previously published human ChIP-seq dataset7. Proteins that bind DNA in a site-specific fashion, such point(s) ...
Sequences of the Nucleocapsid Genes from Two Strains of Avian
... independently derived clones. In the case of Beaudette eight independently derived clones have restriction maps which show that they have this stretch of sequence, all containing a HindlII site at position 1436, which is within the 184 base region (data not shown). The deletion in M41 occurs only fo ...
... independently derived clones. In the case of Beaudette eight independently derived clones have restriction maps which show that they have this stretch of sequence, all containing a HindlII site at position 1436, which is within the 184 base region (data not shown). The deletion in M41 occurs only fo ...
Array CGH for detection of chromosome imbalance
... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
Genome Sequence of an Extremely Halophilic Archaeon
... The genomes of Halobacterium species were originally studied a half-century ago; they are composed of two components, a major fraction that is G+C-rich and a relatively A+T-rich (58% G+C) satellite (5). Subsequent studies showed that the satellite deoxyribonucleic acid (DNA) corresponded mainly to l ...
... The genomes of Halobacterium species were originally studied a half-century ago; they are composed of two components, a major fraction that is G+C-rich and a relatively A+T-rich (58% G+C) satellite (5). Subsequent studies showed that the satellite deoxyribonucleic acid (DNA) corresponded mainly to l ...
West Nile Neuroinvasive Disease Case Definitions Reproduced
... more than 10% of genotypes missing. To minimize the confounding effects likely to be introduced by differences in coverage between the experiments, we restricted both call sets to consensus coding sequences (CCDS), which were included in both our exon capture kit and those used at various 1000 Genom ...
... more than 10% of genotypes missing. To minimize the confounding effects likely to be introduced by differences in coverage between the experiments, we restricted both call sets to consensus coding sequences (CCDS), which were included in both our exon capture kit and those used at various 1000 Genom ...
The Complete Chloroplast and Mitochondrial DNA Sequence of
... variation in gene content, due to massive gene loss, genome erosion, and gene transfer to the nucleus (Grzebyk and Schofield 2003). All cp genomes described so far are circular. Previous studies have shown that, although in green algae (e.g., C. reinhardtii) more genes have been transferred to the n ...
... variation in gene content, due to massive gene loss, genome erosion, and gene transfer to the nucleus (Grzebyk and Schofield 2003). All cp genomes described so far are circular. Previous studies have shown that, although in green algae (e.g., C. reinhardtii) more genes have been transferred to the n ...
Bacteriophages use an expanded genetic code on
... amber stop codons (TAG). No mutations that reached an appreciable frequency (>5%) in the sequenced bacteriophage populations were observed to affect translation termination for four of these genes, which have in-frame non-amber stop codons within 8 codons downstream. Readthrough of the amber stop co ...
... amber stop codons (TAG). No mutations that reached an appreciable frequency (>5%) in the sequenced bacteriophage populations were observed to affect translation termination for four of these genes, which have in-frame non-amber stop codons within 8 codons downstream. Readthrough of the amber stop co ...
Decomposition of DNA Sequence Complexity
... pattern was true for sequences without long-range correlations (Fig. 2). As a result, the complexity differences between both sequences are expected to be maximized when the 兵R, Y 其 mapping rule is used, which is just what other authors have observed [13]. (2) Four-symbol decomposition, allowing one ...
... pattern was true for sequences without long-range correlations (Fig. 2). As a result, the complexity differences between both sequences are expected to be maximized when the 兵R, Y 其 mapping rule is used, which is just what other authors have observed [13]. (2) Four-symbol decomposition, allowing one ...
Supplementary Text 1 (doc 52K)
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
Comparative Genomics of Microbes
... • Tandem repeats: regions of repeated DNA in immediate succession but with different copy number in different genomes. – A repeat can occur 2.5 times October 2K5 ...
... • Tandem repeats: regions of repeated DNA in immediate succession but with different copy number in different genomes. – A repeat can occur 2.5 times October 2K5 ...
Ledbetter Presentation 8/15/05
... the sensitivity and accuracy of CGH-arrays since we detected 100% of all imbalances (n=17) identified by FISH; ...
... the sensitivity and accuracy of CGH-arrays since we detected 100% of all imbalances (n=17) identified by FISH; ...
Brooker Chapter 20
... An individual found to be heterozygous, 7.6/13, is fairly likely to be heterozygous (HbAHbS), and thus a carrier of the mutant allele An individual found to be homozygous, 7.6/7.6, is fairly likely to be homozygous for the normal HbA allele Copyright ©The McGraw-Hill Companies, Inc. Permission requi ...
... An individual found to be heterozygous, 7.6/13, is fairly likely to be heterozygous (HbAHbS), and thus a carrier of the mutant allele An individual found to be homozygous, 7.6/7.6, is fairly likely to be homozygous for the normal HbA allele Copyright ©The McGraw-Hill Companies, Inc. Permission requi ...
The Aspergillus Genome Database, a curated comparative
... veA), the systematic name assigned during the genome sequence assembly and genome annotation (such as AN1052), and any other synonyms or aliases. All names and aliases are searchable, and collection of all of the aliases for each gene ensures that users can find a gene of interest even when confusion ...
... veA), the systematic name assigned during the genome sequence assembly and genome annotation (such as AN1052), and any other synonyms or aliases. All names and aliases are searchable, and collection of all of the aliases for each gene ensures that users can find a gene of interest even when confusion ...
mRNA Expression Analysis
... satisfy the needs of diverse experimental designs. By taking advantage of several innovative underlying technologies, an optimal solution is available regardless of which features are primary, such as highest sample throughput or maximal unbiased discovery. ...
... satisfy the needs of diverse experimental designs. By taking advantage of several innovative underlying technologies, an optimal solution is available regardless of which features are primary, such as highest sample throughput or maximal unbiased discovery. ...
Next Generation Sequencing Panel for Severe Congenital
... system. Sequencing is performed using Illumina technology and reads are aligned to the reference sequence. Variants are identified and evaluated using a custom collection of bioinformatic tools and comprehensively interpreted by our team of directors and genetic counselors. All novel and/or potentia ...
... system. Sequencing is performed using Illumina technology and reads are aligned to the reference sequence. Variants are identified and evaluated using a custom collection of bioinformatic tools and comprehensively interpreted by our team of directors and genetic counselors. All novel and/or potentia ...
Localized hypermutation and associated gene losses in legume
... Carlow, Ireland Point mutations result from errors made during DNA replication or repair, so they are usually expected to be homogeneous across all regions of a genome. However, we have found a region of chloroplast DNA in plants related to sweetpea (Lathyrus) whose local point mutation rate is at l ...
... Carlow, Ireland Point mutations result from errors made during DNA replication or repair, so they are usually expected to be homogeneous across all regions of a genome. However, we have found a region of chloroplast DNA in plants related to sweetpea (Lathyrus) whose local point mutation rate is at l ...
DNA and the Genome
... PCR helps to rapidly identify people. Specific areas of DNA known to vary between individuals is amplified. Giving different sized fragments in different people. CFE Higher Biology ...
... PCR helps to rapidly identify people. Specific areas of DNA known to vary between individuals is amplified. Giving different sized fragments in different people. CFE Higher Biology ...
The Genome of Methanosarcina mazei
... factor of 2.5 (4.09 vs. 1.66 Mbp). This is accounted for by the already mentioned non-coding regions (1.01 Mbp) and by 3,371 ORFs as compared to 1,738 identified ORFs in Mc. jannaschii (Bult et al., 1996). A striking feature of the M. mazei genome is that 1,043 of the 3,371 ORFs have their best blas ...
... factor of 2.5 (4.09 vs. 1.66 Mbp). This is accounted for by the already mentioned non-coding regions (1.01 Mbp) and by 3,371 ORFs as compared to 1,738 identified ORFs in Mc. jannaschii (Bult et al., 1996). A striking feature of the M. mazei genome is that 1,043 of the 3,371 ORFs have their best blas ...
here. - the DeRisi Lab
... between genes that have a high degree of sequence similarity and avoid other problematic regions such as the various types of repetitive sequences or secondary structures. Several competing platforms for producing oligonucleotide-based microarrays have emerged, differing in probe length, number of ...
... between genes that have a high degree of sequence similarity and avoid other problematic regions such as the various types of repetitive sequences or secondary structures. Several competing platforms for producing oligonucleotide-based microarrays have emerged, differing in probe length, number of ...
A proteogenomic toolkit
... Proteomic identifications rely on a comprehensive database in order to perform searches on mass spectra. The available proteomes, even for model organisms, are often incomplete [3]. Furthermore, these reference proteomes can never contain sequences that arise somatically and drive disease. RNA-seq e ...
... Proteomic identifications rely on a comprehensive database in order to perform searches on mass spectra. The available proteomes, even for model organisms, are often incomplete [3]. Furthermore, these reference proteomes can never contain sequences that arise somatically and drive disease. RNA-seq e ...
Chromosomal rearrangements in Salmonella spp. s2-2
... (though not all such loops may function'in pathogenicity); in many cases they include a block of genes contributing to a specific virulence phenotype. Two Salmonella pathogenicity islands ( SPf , both of about 40 kb, have been identified; SPI-I governs the ...
... (though not all such loops may function'in pathogenicity); in many cases they include a block of genes contributing to a specific virulence phenotype. Two Salmonella pathogenicity islands ( SPf , both of about 40 kb, have been identified; SPI-I governs the ...
CRISPR germline engineering—the community
... being edited. In some cases where gene mutations in mice have been studied in mosaics or in chimeras (where two embryos are joined together), the resulting phenotype is worse than when the gene is mutated in all cells. However, generally one expects a milder version of the phenotype. Unanticipated e ...
... being edited. In some cases where gene mutations in mice have been studied in mosaics or in chimeras (where two embryos are joined together), the resulting phenotype is worse than when the gene is mutated in all cells. However, generally one expects a milder version of the phenotype. Unanticipated e ...
Whole genome sequencing
Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.