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Bioinformatics - Department of Computer Science
Bioinformatics - Department of Computer Science

... that do work in the cell. • An open problem is to determine structure computationally from the primary sequence of amino acids. A cartoon of the 3D structure of the myoglobin protein ...
A Comparative Gene Map of the Horse (Equus caballus)
A Comparative Gene Map of the Horse (Equus caballus)

... horse synteny groups established previously with a horse-mouse somatic cell hybrid panel (SCHP, UC Davis). This increased the number of coding genes mapped to the horse genome by over 2-fold and allowed refinements of the comparative mapping data available for this species. In conjunction with 57 pr ...
DNA Sequence Alignment - National Taiwan University
DNA Sequence Alignment - National Taiwan University

... 2.1 The edit distance between two strings We need a way to score an alignment to find the optimal sequence alignment. There is a common way called “edit distance” to measure what is the difference between the two strings. There are four edit operators in the edit distance --- insertion, deletion, re ...
UV-Targeted Dinucleotides Are Not Depleted in Light
UV-Targeted Dinucleotides Are Not Depleted in Light

... (e.g., Naya et al. 2002; Foerstner et al. 2005). In particular, Naya et al. (2002) have shown that aerobic bacteria have a higher G 1 C content than anaerobic bacteria. In addition, aerobic bacteria are more likely to be exposed to sunlight, which means that it could be difficult to distinguish betw ...
MICRO-MANIPULATION OF CHICKEN CHROM OSOMES AND
MICRO-MANIPULATION OF CHICKEN CHROM OSOMES AND

... complement is divided in two distinct groups of 10 macrochromosomes and 29 microchromosomes, for a total of 39 chromosomes per haploid genome. This translates to having about 65 % of the genome contained in the macrochromosomes and 35 % in the microchromosomes. Chromosome 1 contains about 17 % of th ...
Analysis of mRNA - quantitation (contd)
Analysis of mRNA - quantitation (contd)

... caffeine that it would kill any other insect - it is exposed to the equivalent of 500 espressos per day. The closely related species, Hypothenemus eruditus, aka the bark beetle doesn't like coffee and dies rather quickly when fed ground up coffee beans. a) (4 points) Starbucks has funded your lab to ...
Tutorial - QIAGEN Bioinformatics
Tutorial - QIAGEN Bioinformatics

... the reciprocal analysis of methylation, so that both hyper- and hypo-methylated regions can be identified in one workflow. These two instances are arbitrarily named "Call Methylation Levels" and "reverse methylation", and the order of inputs with regard to reads track and control reads track is reve ...
A CRISPR design for next-generation antimicrobials Chase L Beisel
A CRISPR design for next-generation antimicrobials Chase L Beisel

... natural environments, thereby stymying the further dissemination of resistance. Finally, eliminating contamination of batch fermentations without compromising the production host could combat a common and economically costly industrial problem. New ways of addressing this issue without discarding th ...
WALKTHROUGH FOR ANALYSIS OF CODON PAIRING USING
WALKTHROUGH FOR ANALYSIS OF CODON PAIRING USING

... columns (starts in cell A3). This is the observed codon pair data while the lower 64R X 130C block (starts in cell A68) is the expected codon pair count data. The data can be converted into a more manageable form using the spreadsheet ‘Codoncount clean.xls’ Select the entire results.txt worksheet, c ...
gmod-arthrobase-07dec - IUBio Archive for Biology
gmod-arthrobase-07dec - IUBio Archive for Biology

... • GFF Chado, GMODTools, Modware, XORT Chado input and output • LuceGene - Genome object/text search & report ...
Supplementary Methods.
Supplementary Methods.

... Potential scoring bias. Because our motif conservation score is based on the number of conserved motifs, the top predictions tended to be more conserved and longer than the average. Since we had found that longer, more conserved fragments are more likely to function as enhancers in our assay, we con ...
New scientific and technological developments relevant
New scientific and technological developments relevant

... techniques such as aerosols and viral vectors have also made substantial progress. Drugs can be administered through aerosol inhalation, which can be of benefit in reducing recipient non-compliance. Viral vectors offer genetic therapy for many diseases by bringing about fundamental changes to diseas ...
Characterization and transcript mapping of a bovine herpesvirus
Characterization and transcript mapping of a bovine herpesvirus

... approximately 140 kbp which is divided into unique long (UL) and short (Us) regions. The latter is bounded by inverted repeat sequences (Rs) and can invert its orientation relative to UL, resulting in the existence of two isomeric forms (Farley et al., 1981). The genome encodes more than 40 polypept ...
Diapositiva 1
Diapositiva 1

... review, and some aspect of the RefSeq record is predicted. The RefSeq record has not yet been subject to individual review. The initial sequence-to-gene name associations have been established by outside collaborators or NCBI staff. The RefSeq record has been reviewed by NCBI staff or by a collabora ...
Ooplasmic donation in humans The potential for epigenic
Ooplasmic donation in humans The potential for epigenic

... and allele frequencies in the population. Nevertheless, taking into account the striking observations seen in mouse studies is important when debating possible future implications in the adaptation of this technique in the human, particularly as epigenetic modifications may affect future generations ...
- Wiley Online Library
- Wiley Online Library

... of the perturbations in the population(s) must be determined following physical separation of cells into two or more populations. Previously, this was often performed using hybridization techniques such as microarrays, but, more recently, massively parallel sequencing has become the method of choice ...
- ResearchOnline@JCU
- ResearchOnline@JCU

... 15–20% of sporadic colorectal cancer (CRC).2 The serrated polyposis syndrome is associated with CRC risk. Serrated polyposis shows familial clustering,3 and first and seconddegree relatives of index cases with serrated polyposis without CRC are more likely to have been diagnosed with CRC or pancreati ...
Unit 05 - Delivery guide
Unit 05 - Delivery guide

... One form of a gene; e.g. tall and dwarf are the alleles for the height of a pea plant. More than two alleles can exist for any specific gene, but only two of them will be found within any individual. ...
PDF only - at www.arxiv.org.
PDF only - at www.arxiv.org.

... biological objects, expressed, as the universality of their basic functions on the cellular level, as well as on the level of the whole organism. Any process occurring in the organism, including ontogenesis, is primarily determined by the genes of the DNA in each cell. If we consider a multicellular ...
Genomics - Pearson Canada
Genomics - Pearson Canada

... sequence with the sequences of known genes from well-studied species. If the ORF appears to be a gene that has not yet been described in any other species, further research is required before it can actually be considered a gene. A “hit,” in contrast, means that the ORF shares a significant amount o ...
Alternative Splicing Analysis Tools Through the UCSC Genome
Alternative Splicing Analysis Tools Through the UCSC Genome

... output to Galaxy" is checked (BED format consists of four columns: chromosome, start, end, and strand). 6. Hit "get output", and on the following screen hit "Send query to Galaxy", leaving the other parameters as they are. 7. You will be returned to the main page of Galaxy. On the right you can see ...
the list of 56 genes that the American College of Medical Genetics and Genomics
the list of 56 genes that the American College of Medical Genetics and Genomics

... here as incidental findings, the analysis may not be technically equivalent to examining these genes as a primary finding. For example, clinical sequencing could have areas of diminished or absent coverage in the genes examined for incidental findings that would be filled in by Sanger sequencing or ...
Exporter la page en pdf
Exporter la page en pdf

... at 40 kb intervals and fire as small clusters whose synchrony increases during S phase and that replication fork velocity (mean 0.7 kb/min, maximum 2.0 kb/min) remains constant and narrowly distributed through S phase. However, multi-scale analysis of a genome-wide replication timing profile shows a b ...
Advanced Techniques
Advanced Techniques

...  Labeled cDNA hybridizes with DNA on slide ...
Articles (Danaher) ) , short, fluorescently
Articles (Danaher) ) , short, fluorescently

... Homopolymer sequencing accuracy is one of the major challenges of methods with a pyrosequencing-type workflow such as fluorogenic pyrosequencing. The system achieved a single-read raw accuracy of >99%, and 80% of these HL-oligo sequencing traces were error-free. The origins of this accuracy can be v ...
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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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