Precise insertion and guided editing of higher plant
... off target sites within the rice genome, both of which contained a 4 bp mismatch. Both genomic regions were amplified via PCR from multiple GE0001 events as well as from wild type rice. These fragments were subcloned, sequenced via Sanger sequencing and compared to the wild type sequences. No off-ta ...
... off target sites within the rice genome, both of which contained a 4 bp mismatch. Both genomic regions were amplified via PCR from multiple GE0001 events as well as from wild type rice. These fragments were subcloned, sequenced via Sanger sequencing and compared to the wild type sequences. No off-ta ...
Adaptation to nocturnality - learning from avian genomes
... such that using equal or longer scaffolds produces half the bases of the assembly. That is the N50 value denotes that 50% of the entire assembly length is contained in scaffolds that have an equal or bigger length than N50), which measures assembly contiguity [13]. Most currently assembled genomes a ...
... such that using equal or longer scaffolds produces half the bases of the assembly. That is the N50 value denotes that 50% of the entire assembly length is contained in scaffolds that have an equal or bigger length than N50), which measures assembly contiguity [13]. Most currently assembled genomes a ...
1995 Broad et al: CURRENT STATE OF THE NEW ZEALAND
... The increased fecundity of sheep with the Booroola gene is attributable to a mutation at a single locus which segregates in the classical Mendelian fashion. However, it is known that many of the production and performance traits segregate in a more complex, quantitative manner. The “whole genome scr ...
... The increased fecundity of sheep with the Booroola gene is attributable to a mutation at a single locus which segregates in the classical Mendelian fashion. However, it is known that many of the production and performance traits segregate in a more complex, quantitative manner. The “whole genome scr ...
Slide 1
... Frequencies of individual alleles also do not tell us the whole story. Indeed, distributions of alleles of different loci (states of different traits) can dependent on each other. Independent joint distribution of alleles at several loci means that the frequency of a genotype is equal to the produc ...
... Frequencies of individual alleles also do not tell us the whole story. Indeed, distributions of alleles of different loci (states of different traits) can dependent on each other. Independent joint distribution of alleles at several loci means that the frequency of a genotype is equal to the produc ...
Supplemental Material
... PF00059) and SMART (http://smart.embl-heidelberg.de/; SRCR: SM00202; CTLD: SM00034) databases (SCHULTZ et al. 1998; SONNHAMMER et al. 1998). Only protein domains identified by at least two domain identification databases were scored as positive. The presence of accessory domains in these proteins wa ...
... PF00059) and SMART (http://smart.embl-heidelberg.de/; SRCR: SM00202; CTLD: SM00034) databases (SCHULTZ et al. 1998; SONNHAMMER et al. 1998). Only protein domains identified by at least two domain identification databases were scored as positive. The presence of accessory domains in these proteins wa ...
Identification of large-scale human-specific copy number
... et al. 2003; Liu et al. 2003; Fortna et al. 2004). Furthermore, copy number differences (CNDs) on the scale of up to hundreds of kilobases have also been described (Locke et al. 2003). As a means to establish the frequency of such largescale CNDs, inter-hominoid array comparative genomic hybridizatio ...
... et al. 2003; Liu et al. 2003; Fortna et al. 2004). Furthermore, copy number differences (CNDs) on the scale of up to hundreds of kilobases have also been described (Locke et al. 2003). As a means to establish the frequency of such largescale CNDs, inter-hominoid array comparative genomic hybridizatio ...
Supplemental file S8 to
... (“PDZomes”) of several vertebrate and invertebrate species. PDZ domains are indentified by various databases (e.g., SMART and Pfam; see Material Methods), but a caveat often associated with these automated compilations are a high degree of redundancy and the presence of (predicted) genes and various ...
... (“PDZomes”) of several vertebrate and invertebrate species. PDZ domains are indentified by various databases (e.g., SMART and Pfam; see Material Methods), but a caveat often associated with these automated compilations are a high degree of redundancy and the presence of (predicted) genes and various ...
How Does Replication-Associated Mutational Pressure Influence
... Genome Research www.genome.org ...
... Genome Research www.genome.org ...
Mitochondrial DNA Mutations and Disease
... in which, the NADH and FADH2 produced through TCA cycle and fatty acid betaoxidation in mitochondria are oxidized. Electrons from these reducing agents are going through the electron transport chain and finally given to oxygen, creating a protongradient, which drives ATP synthesis. Most patients wit ...
... in which, the NADH and FADH2 produced through TCA cycle and fatty acid betaoxidation in mitochondria are oxidized. Electrons from these reducing agents are going through the electron transport chain and finally given to oxygen, creating a protongradient, which drives ATP synthesis. Most patients wit ...
BMC Genomics - LCBB
... d(G1, G2) as the minimum number of events required to transform one genome into the other. The breakpoint distance [17] is not a direct evolutionary distance measurement. A breakpoint in G1 is defined as an ordered pair of genes (gi, gj) such that gi and gj are adjacent in G1 but not in G2. The brea ...
... d(G1, G2) as the minimum number of events required to transform one genome into the other. The breakpoint distance [17] is not a direct evolutionary distance measurement. A breakpoint in G1 is defined as an ordered pair of genes (gi, gj) such that gi and gj are adjacent in G1 but not in G2. The brea ...
![P[acman]: A BAC Transgenic Platform for Targeted Insertion of](http://s1.studyres.com/store/data/015771109_1-230af42a78d308ddb0e06d89019d2ef2-300x300.png)
P[acman]: A BAC Transgenic Platform for Targeted Insertion of
... copy number, facilitating plasmid maintenance and recombineering, but is induced to high copy number for plasmid isolation. Recombineering allows gap repair and mutagenesis in bacteria. Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones. fC31mediated transg ...
... copy number, facilitating plasmid maintenance and recombineering, but is induced to high copy number for plasmid isolation. Recombineering allows gap repair and mutagenesis in bacteria. Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones. fC31mediated transg ...
(CCDG CVD) Working Group Plan - Centers for Common Disease
... each gene, containing many hundreds of bases pinpointed with high specificity - in effect, a noncoding equivalent of the exons of a gene. To do so, we will exploit rich sources of information about non-coding regions. (4) RVAS across the genome: Structural variation. Structural variants (SVs) - incl ...
... each gene, containing many hundreds of bases pinpointed with high specificity - in effect, a noncoding equivalent of the exons of a gene. To do so, we will exploit rich sources of information about non-coding regions. (4) RVAS across the genome: Structural variation. Structural variants (SVs) - incl ...
Milestone2
... Many eukaryotic genes contain a DNA sequence, called a TATA box, in their promoter region, i.e., just upstream of their start codon. The TATA box is so named because it often consists of the following hexamer (i.e., 6 nucleotide) motif: “TATAAA”. Write a Python program named TATA.py, which reads in ...
... Many eukaryotic genes contain a DNA sequence, called a TATA box, in their promoter region, i.e., just upstream of their start codon. The TATA box is so named because it often consists of the following hexamer (i.e., 6 nucleotide) motif: “TATAAA”. Write a Python program named TATA.py, which reads in ...
htr1A - Utrecht University Repository
... 5-HT functions as a hormone, a mitogen, and a neurotransmitter. Its significance is not only evident from its evolutionary age but also demonstrated by the fact that it is used by one of the most extensive signaling systems found in the brain: from the raphe nuclei of the brain stem, serotonergic ne ...
... 5-HT functions as a hormone, a mitogen, and a neurotransmitter. Its significance is not only evident from its evolutionary age but also demonstrated by the fact that it is used by one of the most extensive signaling systems found in the brain: from the raphe nuclei of the brain stem, serotonergic ne ...
Preparation of PCR Products for DNA Sequencing
... into the Gel Nebulizer inside the Micropure separator (Figure 1). The device was capped and centrifuged for 10 min at 14 000× g. The filtrate from Micropure was sequenced without further purification or concentration. The agarose gels were prepared in different electrophoresis buffers to assess buff ...
... into the Gel Nebulizer inside the Micropure separator (Figure 1). The device was capped and centrifuged for 10 min at 14 000× g. The filtrate from Micropure was sequenced without further purification or concentration. The agarose gels were prepared in different electrophoresis buffers to assess buff ...
Analysis of SV - Genome Analysis Wiki
... Mappers will not be able to place reads correctly in the inversion, or across the breakpoints ...
... Mappers will not be able to place reads correctly in the inversion, or across the breakpoints ...
Medical Genetics: An Overview
... resorted to in managing patients and carriers of these diseases. The wide spectrum of medical genetics includes six main fields: basic, clinical, diagnostic, prophylactic, therapeutic and applied genetics. The scopes of most of these fields are self-explanatory with few exceptions. Pathogenetics, a ...
... resorted to in managing patients and carriers of these diseases. The wide spectrum of medical genetics includes six main fields: basic, clinical, diagnostic, prophylactic, therapeutic and applied genetics. The scopes of most of these fields are self-explanatory with few exceptions. Pathogenetics, a ...
Schedulling - GEOCITIES.ws
... programming can find the lowest-cost mix of ingredients, and the economic order quantity with noninstantaneous replenishment can determine the optimum length of a production run. For mass production, the schedule of production is pretty much determined when the assembly line is laid out. Products si ...
... programming can find the lowest-cost mix of ingredients, and the economic order quantity with noninstantaneous replenishment can determine the optimum length of a production run. For mass production, the schedule of production is pretty much determined when the assembly line is laid out. Products si ...
Query Results
... I.- Genome Explore Module: This module allows the user to browse and explore a genome, in terms of the context where potential or virtual SAGE tags are found for a given anchoring-tagging enzyme pair. ...
... I.- Genome Explore Module: This module allows the user to browse and explore a genome, in terms of the context where potential or virtual SAGE tags are found for a given anchoring-tagging enzyme pair. ...
Genome Analysis Excerpt from Chapter 11
... (prokaryotes). The size and number of introns in particular genes can be tracked in the genomes of closely related species. The function of introns is not known, but it has been suggested that they play an important role in genome evolution of eukaryotic organisms. The birth of new introns and the d ...
... (prokaryotes). The size and number of introns in particular genes can be tracked in the genomes of closely related species. The function of introns is not known, but it has been suggested that they play an important role in genome evolution of eukaryotic organisms. The birth of new introns and the d ...
The Difference Makers
... messing with the genome? [Yes, but it would probably be slower, relying on ordinary mutations that arise during DNA replication when cells divide or genetic recombination of chromosomes in a new generation.] 5. Why do transposons appear to have been important for the evolution of the immune system? ...
... messing with the genome? [Yes, but it would probably be slower, relying on ordinary mutations that arise during DNA replication when cells divide or genetic recombination of chromosomes in a new generation.] 5. Why do transposons appear to have been important for the evolution of the immune system? ...
Construction of a set of convenient saccharomyces cerevisiae
... replacement. For each marker, we determined that for the European Union Yeast Genome Sequencthe auxotrophy segregated 2 : 2 in tetrads (data not ing Programme. This DNA has been or is curshown) and confirmed the gene replacement by rently being used for sequencing chromosomes Southern analysis (data ...
... replacement. For each marker, we determined that for the European Union Yeast Genome Sequencthe auxotrophy segregated 2 : 2 in tetrads (data not ing Programme. This DNA has been or is curshown) and confirmed the gene replacement by rently being used for sequencing chromosomes Southern analysis (data ...
ch4-TheGenomicBiologistsToolKit_1.3
... The typical simple cloning vector will accommodate DNA fragments up to about 3,000 bp in length. However, there are needs to clone significantly longer fragments of DNA for study. Typically DNA genomic sequencing is easiest with the longest fragments possible. Two vector systems, i.e. BAC vectors (b ...
... The typical simple cloning vector will accommodate DNA fragments up to about 3,000 bp in length. However, there are needs to clone significantly longer fragments of DNA for study. Typically DNA genomic sequencing is easiest with the longest fragments possible. Two vector systems, i.e. BAC vectors (b ...
CH4. The Genomic Biologists Toolkit
... The typical simple cloning vector will accommodate DNA fragments up to about 3,000 bp in length. However, there are needs to clone significantly longer fragments of DNA for study. Typically DNA genomic sequencing is easiest with the longest fragments possible. Two vector systems, i.e. BAC vectors (b ...
... The typical simple cloning vector will accommodate DNA fragments up to about 3,000 bp in length. However, there are needs to clone significantly longer fragments of DNA for study. Typically DNA genomic sequencing is easiest with the longest fragments possible. Two vector systems, i.e. BAC vectors (b ...
Whole genome sequencing
Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.