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Part 2 - Laboratory of Aquaculture & Artemia Reference Center
Part 2 - Laboratory of Aquaculture & Artemia Reference Center

... system for its survival even under extreme conditions. Artemia seems to be a promising system for stress response studies as observed in our laboratory since it shows a swift adaptation to harsh environments. A variety of Heat shock proteins (HSPs) were expressed which when characterized will give a ...
ppt - University of Illinois at Urbana
ppt - University of Illinois at Urbana

... • What is the evolutionary scenario for transforming one genome into the other? ...
Study of Hypertension in Spontaneous Hypertensive Rats by
Study of Hypertension in Spontaneous Hypertensive Rats by

... Briefly, 4 hypertensive SHR, 2 normotensive Wistar-Kyoto rats, 1 normotensive Dahl-S and 1 normotensive Dahl-R rat, all aged 18-20 weeks and weighing 320-350g, were anaesthetised with pentobarbital (50 mg/kg intra-peritoneal) and 3 ml blood was taken from the descending aorta of each animal. In the ...
click to - White Rose Research Online
click to - White Rose Research Online

... recent study produced low coverage sequence data for 199 B. rapa and 119 B. oleracea accessions to identify SNPs and trace parallel selection signals in the two subgenomes (Cheng et al. 2016). Regions undergoing positive selection could be identified (25 in B. rapa and 58 in B. oleracea), out of whi ...
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual

... et al. 2012), and Vidania fulgoroideae of fulgorid planthoppers (Gonella et al. 2011) form a clade within Betaproteobacteria in 16S rDNA-based phylogenies. This BetaSymb lineage has been proposed to represent the ancestral partner of Sulcia that has been replaced in several descendant lineages of th ...
Heterogeneity of Genome and Proteome Content in Bacteria
Heterogeneity of Genome and Proteome Content in Bacteria

... investigate the rigor and value of the current classification system, it is, therefore, of interest to catalog important genes and proteins in each domain and to show strong similarities and differences within and between domains. In this post-genomic era we can, in principle, study the complete inve ...
Andrew Farmer NCGR Paul Scott Iowa State Bill
Andrew Farmer NCGR Paul Scott Iowa State Bill

... • Variation in genotypic expression of the ramosa genes in the ear shoot spikeletpair meristem is highly correlated with genotypic variation in kernels per row in the mature ear. • Reactive oxygen species may serve as a signal acting on a gene network centered on C/EBP and CREB that regulates initia ...
Application of whole genome sequencing to fully characterise
Application of whole genome sequencing to fully characterise

... bacterial pathogen amongst patients presenting to primary care. Although there was little variation in the burden of illness between the two studies, the molecular epidemiology of the Campylobacter isolates from these studies has not been investigated previously. The aim of this study was to use who ...
Defining a pipeline to use a next generation sequencing
Defining a pipeline to use a next generation sequencing

... load is decreased thanks to the bypass of visual classification of variants detected in ...
Chapter 5. Homology 3D Structure Prediction Chapter 6. Ab Initio
Chapter 5. Homology 3D Structure Prediction Chapter 6. Ab Initio

... (SSEs matching, residue environment matching) Post-processing of the results need due to the low accuracy (50%) finding the correct fold (filtering by other predictions or known experimental data) Goal From native fold approximation of the energy or part of it and comparison with the energy of the n ...
Genome Research 17
Genome Research 17

... Consortium (2004), while the zebra finch has 2n = 80 (Pigozzi and Solari 1999). As judged from the analysis of the chicken genome sequence, the microchromosomes differ from the larger chromosomes by showing higher gene density, fewer repeats, shorter introns, higher GC content, and much higher recom ...
UK Statutory Basis for and Judicial Application of a Utility
UK Statutory Basis for and Judicial Application of a Utility

... understood, and no disease has been identified which is attributable to an excess or a deficiency of it, and no other practical use is suggested for it, then the requirement of industrial applicability is not satisfied Using the claimed invention to find out more about its own activities is not in i ...
Supplementary Information (docx 2885K)
Supplementary Information (docx 2885K)

... 5. Alternative strategy for filtering rare variants Base on previous knowledge of highly genetic heterogeneity9 and the contribution of rare variants in schizophrenia10-12, we focus on the difference of very rare variants distribution between cases and controls. So we design a personality analysis p ...
Slides on chromosomal changes
Slides on chromosomal changes

... isolation between species. 2) A number of crop plants have undergone such changes and chromosome manipulation may be important in agriculture (breeding). 3) A number of such changes are responsible for human genetic diseases. 4) They may disrupt gene function directly if a break occurs in a gene. 5) ...
Expanding Yeast Knowledge Online.
Expanding Yeast Knowledge Online.

... Genetics, Stanford University, Stanford, CA 94305-5120, U.S.A. Tel: 1-650-723-7541; fax: 1-650-723-7016; e-mail: [email protected] ...
Hb Malmö [ß-97(FG-4)His]Gln] leading to polycythemia in a
Hb Malmö [ß-97(FG-4)His]Gln] leading to polycythemia in a

... decreases of MCH and MCV. At any given PCV value, whole-blood viscosity is not influenced by the volume and hemoglobin content of red cells [20]. However, at identical packed red cell volumes, patients with a low MCH have also lower Hb and oxygen-carrying capacity. This is likely to be detrimental i ...
Enthusiasm mixed with scepticism about single
Enthusiasm mixed with scepticism about single

... dissecting the genetics behind complex diseases. A number of more specific questions remain contentious, however, including how to ensure that information about human genetic variation is made widely available, how best to ascertain such markers in large patient cohorts and, figuring prominently at ...
Whole genome analyses using PopGenome and VCF files
Whole genome analyses using PopGenome and VCF files

... This module provides a wide range of FST as well as diversity measurenments. There exists two main classes. First, calculations which are either based on haplotypes mode=ḧaplotype¨ or second, the sequence based methods focussing on nucleotides mode="nucleotide". Note, be careful with haplotype base ...
Day 1. General aspects for genetic map construction
Day 1. General aspects for genetic map construction

... SOLiD (Sequencing by Ligation), Illumina (Sequencing by synthesis), 454 (Pyrosequencing) ...
Investigating a Eukaryotic Genome
Investigating a Eukaryotic Genome

... contains no insert. By using this control in a transformation reaction, they can get a background reading of the frequency of vector reclosure without insert by counting any resulting colonies. Since the vector is treated with phosphatase, this background is usually very low. Sample data is provided ...
How to submit samples - McGill University and Génome Québec
How to submit samples - McGill University and Génome Québec

... Primer names must be entered in the ‘Primer’ column. Only one primer is permitted per cell. If a sample is to be sequenced with more than one primer then that sample name must be entered in the ‘Sample name’ column as many times as it needs to be sequenced. Then all associated primers must be listed ...
The UCSC Genome Browser
The UCSC Genome Browser

... choose a mouse assembly in the Genome Browser Gateway and enter ADAM2 in the position field, just as we did for human. However, this approach is risky, since orthologs do not always have the same names. In this case, it turns out that the intuitive approach gives you a clue as to where the mouse ort ...
MCB 371/372 - Gogarten Lab | UConn
MCB 371/372 - Gogarten Lab | UConn

... (frequencies) and one with final data (integer scores). Blastpgp can only use the intermediate data scoremats, and unfortunately the scoremats on the ftp side are final data scoremats. We are in the process of trying to make this easier, perhaps by placing the intermediate scoremats on the ftp site ...
The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is
The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is

... rubripes (Elgar et al. 1996) and is about four times as small as the human genome. Xiphophorus genomic DNA contains approximately 90% single-copy sequences, with the remaining 10% being predominantly multiple-copy elements (Schwab 1982). Except for the D locus, which is repeated as 20–50 copies per ...
towards synthetic plant genomes, transcriptomes and epigenomes
towards synthetic plant genomes, transcriptomes and epigenomes

... Received 6 November 2015; revised 23 November 2015; accepted 25 November 2015. *For correspondence (e-mail [email protected]). ...
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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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