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"Using Model Organism Databases (MODs)". In: Current - SGD-Wiki
"Using Model Organism Databases (MODs)". In: Current - SGD-Wiki

... This unit provides basic protocols for accessing information about genes in MODs. The growing number of MODs and the various types of data and analysis tools available from them cannot all be covered in this unit. The aim of this set of protocols is to provide a general introduction to enable the n ...
Rice Bioinformatics. Analysis of Rice Sequence Data and
Rice Bioinformatics. Analysis of Rice Sequence Data and

... of total bases of DNA/RNA (ftp://ncbi.nlm.nih. gov/GenBank/gbrel.txt; GenBank release 120.0). With the completion of the Arabidopsis genome, plant biologists will have the opportunity to assess the entire gene complement of a plant for the first time. New avenues of research have begun that will cul ...
Figure S1 The yellow color of the Mimulus lewisii nectar
Figure S1 The yellow color of the Mimulus lewisii nectar

... GUIDELESS, the more SL9 reads will be found. Upon reaching a point that is completely unlinked with GUIDELESS, the two genotypes (LF10 and SL9) will be randomly segregating in an expected proportion of 50%:50%. The 157,551 raw SNPs were first filtered by depth of coverage. SNPs with >120-fold covera ...
New Perspectives on Rickettsial Evolution from New
New Perspectives on Rickettsial Evolution from New

... A wide variety of repetitive sequence elements are found in bacteria. These range from duplicated genes which exhibit varying degrees of homology like the ATP/ADP translocase genes, proline-betaine transporters, and sca gene families in Rickettsia, to the small palindromic repeat elements which are ...
Touring Ensembl: A practical guide to genome browsing Open Access
Touring Ensembl: A practical guide to genome browsing Open Access

... Cambs, CB10 1SD, UK Full list of author information is available at the end of the article ...
Extracting Haplotypes from Diploid Organisms
Extracting Haplotypes from Diploid Organisms

... groups of organisms, from viruses to prokaryotic bacteria and archaea, from simple microscopic eukaryotes to complex large plants and animals. At present, by far the biggest increase has come from human population studies. Over 10 million common single nucleotide polymorphisms (SNPs) have been ident ...
Genomic Databases for Tomato
Genomic Databases for Tomato

... Information concerning various biological aspects such as functional annotations of genes and gene products, intron-exon structures, gene expressions and metabolic pathways is available from various tomatorelated web sites. These public data advance not only functional genomics but also the emerging ...
Sequencing Grade Modified Trypsin, Frozen, Product Information
Sequencing Grade Modified Trypsin, Frozen, Product Information

... Description: Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC peptide analysis. In addition, auto-proteolysis can result in th ...
Glencoe Biology
Glencoe Biology

...  The ends of the DNA fragments, called sticky ends, contain singlestranded DNA that is ...
Pyrosequencing Technology
Pyrosequencing Technology

... which is used instead of dATP. This enzyme also hydrolyzes ATP. • The rate of dNTP degradation by apyrase is slower than the rate of dNTP incorporation by the polymerase, favouring sufficient incorporation of dNTPs. • The rate of ATP synthesis by the sulfurylase is faster than the rate of ATP hydrol ...
Potential of promotion of alleles by genome editing for improving
Potential of promotion of alleles by genome editing for improving

... Overall hypothesis of GS2.0 • Sequence data has huge potential in livestock and plant breeding • Huge volumes of sequence needed to realize ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
Comparison of Sample Sequences of the Genome to the Sequence K-12

... from their common ancestor, and these regions share an average of about 85% identity at the nucleotide level and are even more conserved at the amino acid level (50). Previous studies used discrepancies in the alignments of the genetic maps of Salmonella and E. coli or DNA-DNA hybridization between ...
XSL Formatter - H:\XML
XSL Formatter - H:\XML

... What defines a set? Environmental sample, population, phylogenetic, and mutation sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from diffe ...
Inheritance of Organelle DNA Sequences in a Citrus–Poncirus
Inheritance of Organelle DNA Sequences in a Citrus–Poncirus

... that these configurations resulted from influence of the nuclear genome. Nuclear alleles might alter mtDNA organization in the progeny, producing P. trifoliata configurations. Alternatively, the P. trifoliata mtDNA configurations observed in the progeny might result from nuclear copies of these mito ...
Homologous and Nonhomologous Rearrangements: Interactions
Homologous and Nonhomologous Rearrangements: Interactions

... The Aevol model was developed in our team to study the evolution of genome structure. It simulates the evolution of a population of N artificial haploid organisms with flexible genomes. Although a description of the model has already been published (see Knibbe et al. (2008) and its supp. mat.), we t ...
Chromosomal Microarray (CGH+SNP)
Chromosomal Microarray (CGH+SNP)

... There are two reasons for this: 1) UPD may be of the heterodisomy type, which is not detected by the array; and 2) for some of the disorders, mechanisms other than UPD can cause the disorder.  Failure to detect ROH does not exclude the clinical diagnosis of a recessive disorder.  Failure to dete ...
Analysis of Flanking Sequences from Dissociation
Analysis of Flanking Sequences from Dissociation

... quency of intrachromosomal transpositions. Similar observations were made with Ac element transposition in maize (Dooner et al., 1994), where many genetically unlinked transpositions in fact proved to have occurred within the same chromosome as that occupied by the donor site. The overall lower numb ...
Analysis of Flanking Sequences from Dissociation
Analysis of Flanking Sequences from Dissociation

... quency of intrachromosomal transpositions. Similar observations were made with Ac element transposition in maize (Dooner et al., 1994), where many genetically unlinked transpositions in fact proved to have occurred within the same chromosome as that occupied by the donor site. The overall lower numb ...
Basic Genetics and Genomics: A Primer for Nurses
Basic Genetics and Genomics: A Primer for Nurses

... common cancers. Genome-wide association studies (GWAS) are one such research approach. GWAS involve the use of gene chips that can look at the complete sets of variable DNA markers (up to about a million!) in many people to find genetic variations associated with a particular disease. When these new ...
Genetic Disorder Powerpoint Project
Genetic Disorder Powerpoint Project

... Objective: To incorporate your knowledge about cells, cell division, genetics, and DNA to research and present a 12 slide presentation using multimedia presentation software such as PowerPoint on a specific genetic disorder. Background knowledge: You have already completed your basic study about the ...
Investigation 1: Identify the Transcriptional Unit
Investigation 1: Identify the Transcriptional Unit

... Termination of mRNA transcription is different in eukaryotes than in prokaryotes. In eukaryotes, RNA polymerase II passes through one or more AATAAA sequences, which lie beyond the 3' end of the coding region (i.e. thick black boxes in the FlyBase Genes track). The pre-mRNA molecule will thus carry ...
Comparison between Human and Mouse genomes
Comparison between Human and Mouse genomes

... Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in g ...
Genetic Disorder Powerpoint Project
Genetic Disorder Powerpoint Project

... Objective: To incorporate your knowledge about cells, cell division, genetics, and DNA to research and present a 12 slide presentation using multimedia presentation software such as PowerPoint on a specific genetic disorder. Background knowledge: You have already completed your basic study about the ...
module 2: transcription part i
module 2: transcription part i

... Termination of mRNA transcription is different in eukaryotes than in prokaryotes. In eukaryotes, RNA polymerase II passes through one or more AATAAA sequences, which lie beyond the 3' end of the coding region (i.e. thick black boxes in the FlyBase Genes track). The pre-mRNA molecule will thus carry ...
Intra-isolate genome variation in arbuscular mycorrhizal
Intra-isolate genome variation in arbuscular mycorrhizal

... recombination is rare, the failure to purge deleterious mutations could lead to the presence of pseudogenes. This process is known as Muller’s ratchet (Muller, 1964). Second, if multiple gene copies within the AMF ...
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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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