Bacteriophages
... Vectors based on Bacteriophage λ The λ genome is 48.5 kb, in which 15 kb or so is ‘optional’ it contains genes that are only needed for integration into the E. coli chromosome (controlling lysogenic properties) These segments can therefore be deleted without impairing the ability of the phage to in ...
... Vectors based on Bacteriophage λ The λ genome is 48.5 kb, in which 15 kb or so is ‘optional’ it contains genes that are only needed for integration into the E. coli chromosome (controlling lysogenic properties) These segments can therefore be deleted without impairing the ability of the phage to in ...
1 - marric.us
... 31. What are the differences between a prokaryotic and eukaryotic cell? (pg 185-186) 32. Make a sketch of the nitrogen cycle. (pg 48) 33. Describe how cells change when placed in isotonic, hypertonic and hypotonic solutions. (pg 204-205) 34. Compare active transport with passive transport. (pg 201- ...
... 31. What are the differences between a prokaryotic and eukaryotic cell? (pg 185-186) 32. Make a sketch of the nitrogen cycle. (pg 48) 33. Describe how cells change when placed in isotonic, hypertonic and hypotonic solutions. (pg 204-205) 34. Compare active transport with passive transport. (pg 201- ...
Microbiology Exam II - University of Evansville Faculty Web sites
... 6. Which of the following best describes a plasmid? a. A gene within the chromosome b. Small circular piece of DNA outside the chromosome c. The genetic material of a bacteriophage d. Part of bacterial ribosomes e. A single, linear strand of DNA 7. Which of the following is NOT involved in bacteria ...
... 6. Which of the following best describes a plasmid? a. A gene within the chromosome b. Small circular piece of DNA outside the chromosome c. The genetic material of a bacteriophage d. Part of bacterial ribosomes e. A single, linear strand of DNA 7. Which of the following is NOT involved in bacteria ...
Ch 10
... The similarity in the amino acid sequences of the various globin proteins supports this model of gene duplication and mutation ...
... The similarity in the amino acid sequences of the various globin proteins supports this model of gene duplication and mutation ...
Small-Molecule Detection and Enantiopurity Measurement using
... enabling them to direct nearly all of the processes that make life possible. These capabilities have been fine-tuned by billions of years of evolution, and more recently, have been harnessed in the laboratory to enable the use of DNA and RNA for applications that are completely unrelated to their ca ...
... enabling them to direct nearly all of the processes that make life possible. These capabilities have been fine-tuned by billions of years of evolution, and more recently, have been harnessed in the laboratory to enable the use of DNA and RNA for applications that are completely unrelated to their ca ...
Characterization of head-hunter proteins for exchange of genetic information between cells.
... environment. This is particularly prevalent in bacteria as they exchange DNA molecules like plasmids at high frequencies; and this is important for bacteria to acquire resistance to antibiotics. The machinery for such an exchange of genetic information is highly conserved among bacteria. During evol ...
... environment. This is particularly prevalent in bacteria as they exchange DNA molecules like plasmids at high frequencies; and this is important for bacteria to acquire resistance to antibiotics. The machinery for such an exchange of genetic information is highly conserved among bacteria. During evol ...
Final Review
... 31. 3 bases together are called a ____________________. Each of these code for one ____________________________, which string together to form ________________________. Two or more of these make a _______________________. 32. Name 3 types of mutations. 33. What is recombinant DNA? How are scientists ...
... 31. 3 bases together are called a ____________________. Each of these code for one ____________________________, which string together to form ________________________. Two or more of these make a _______________________. 32. Name 3 types of mutations. 33. What is recombinant DNA? How are scientists ...
Microarrays = Gene Chips
... 6. Wash off any unstuck PCR products 7. Use a laser to detect the fluorescent dyes and create a visual image of the pattern of the dyes 8. If the PCR product has stuck on it will glow 9. The computer can then say which of the bacterial species the PCR products have stuck to and this indicates which ...
... 6. Wash off any unstuck PCR products 7. Use a laser to detect the fluorescent dyes and create a visual image of the pattern of the dyes 8. If the PCR product has stuck on it will glow 9. The computer can then say which of the bacterial species the PCR products have stuck to and this indicates which ...
File - MRS. WILSON Science
... of replication. The double helix unzips in both directions along the strand. Eukaryotic chromosomes are very long, so they have many origins of replication to help speed the process. Other proteins hold the two strands apart. • The unzipping exposes the bases on the DNA strands and enables free-floa ...
... of replication. The double helix unzips in both directions along the strand. Eukaryotic chromosomes are very long, so they have many origins of replication to help speed the process. Other proteins hold the two strands apart. • The unzipping exposes the bases on the DNA strands and enables free-floa ...
8-3 Notes with Power point
... ___________________________copies the genetic information. A single strand of DNA serves as a template for a new strand. The rules of base pairing direct replication. DNA is replicated during the ______________________________stage of the cell cycle. Each body cell gets a complete set of ___________ ...
... ___________________________copies the genetic information. A single strand of DNA serves as a template for a new strand. The rules of base pairing direct replication. DNA is replicated during the ______________________________stage of the cell cycle. Each body cell gets a complete set of ___________ ...
Genetics
... Categorize the different kinds of mutations that can occur in DNA Compare the effects of different kinds of mutations on cells and organisms. ...
... Categorize the different kinds of mutations that can occur in DNA Compare the effects of different kinds of mutations on cells and organisms. ...
Fertilisation, development and DNA
... I can state that human males produce sex cells called sperm and human females produce sex cells called eggs. I can label and describe the structures and functions of the main parts of the reproductive organs i.e. ovary, testes, vagina, penis, uterus, oviduct and sperm. I can state that both sex cell ...
... I can state that human males produce sex cells called sperm and human females produce sex cells called eggs. I can label and describe the structures and functions of the main parts of the reproductive organs i.e. ovary, testes, vagina, penis, uterus, oviduct and sperm. I can state that both sex cell ...
Copying DNA: Southern Blotting
... 1 DNA is heated into separate strands 2 The mixture is cooled, and primers bind to strands 3 DNA polymerase adds nucleotides to strands, producing two ...
... 1 DNA is heated into separate strands 2 The mixture is cooled, and primers bind to strands 3 DNA polymerase adds nucleotides to strands, producing two ...
Unit 4: DNA, RNA and Protein Synthesis
... • anticodon results in the • Chargaff’s transmission Rule and/or • chromosomes conservation of • codon the genetic • complimentary information. strand • Explain the • cytosine structural • deletion relationships • deoxyribonucle between DNA, ic acid (DNA) genes, and • deoxyribose chromosomes. • DNA ...
... • anticodon results in the • Chargaff’s transmission Rule and/or • chromosomes conservation of • codon the genetic • complimentary information. strand • Explain the • cytosine structural • deletion relationships • deoxyribonucle between DNA, ic acid (DNA) genes, and • deoxyribose chromosomes. • DNA ...
00_BioBackground
... The corresponding amino acid is then added to a growing chain that comprise the protein ...
... The corresponding amino acid is then added to a growing chain that comprise the protein ...
Lecture 2
... in rep of pMB1; (2) bla gene, coding for beta-lactamase that confers resistance to ampicillin (source – plasmid pBR322); (3) region of E.coli operon lac containing CAP protein binding site, promoter Plac, lac repressor binding site and 5’-terminal part of the lacZ gene encoding the N-terminal fragme ...
... in rep of pMB1; (2) bla gene, coding for beta-lactamase that confers resistance to ampicillin (source – plasmid pBR322); (3) region of E.coli operon lac containing CAP protein binding site, promoter Plac, lac repressor binding site and 5’-terminal part of the lacZ gene encoding the N-terminal fragme ...
Document
... One of the basic tools of modern biotechnology is DNA restriction enzyme digestion. This is the process of cutting DNA into smaller fragments using enzymes. This allows scientists to analyze and compare DNA from different sources, and to study the smaller more manageable pieces of DNA. Becau ...
... One of the basic tools of modern biotechnology is DNA restriction enzyme digestion. This is the process of cutting DNA into smaller fragments using enzymes. This allows scientists to analyze and compare DNA from different sources, and to study the smaller more manageable pieces of DNA. Becau ...
Document
... D.) No difference in transcription rate when an activator protein was present. E.) Negative control of transcription. ...
... D.) No difference in transcription rate when an activator protein was present. E.) Negative control of transcription. ...
A document that can help for writing your lab report: www
... Plasmids used in genetic engineering are called vectors. They are used to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype that can be selected for or against. Most also contain a polylinker or multiple cloning site (MCS), which is a sh ...
... Plasmids used in genetic engineering are called vectors. They are used to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype that can be selected for or against. Most also contain a polylinker or multiple cloning site (MCS), which is a sh ...
1.3 Ten themes unify the study of life
... Evolution= generation-to-generation change in the proportion of different inherited genes in a population ...
... Evolution= generation-to-generation change in the proportion of different inherited genes in a population ...
Genetics
... Relate the concept of the gene to the sequences of nucleotides in DNA Sequence the steps involving protein synthesis Categorize the different kinds of mutations that can occur in DNA Compare the effects of different kinds of mutations on cells and organisms. ...
... Relate the concept of the gene to the sequences of nucleotides in DNA Sequence the steps involving protein synthesis Categorize the different kinds of mutations that can occur in DNA Compare the effects of different kinds of mutations on cells and organisms. ...
CHAPTER 13 Frontiers of Genetics
... repressor turns the operator off by binding to it. This process enables prokaryotes to match their cell chemistry to different conditions. Eukaryotic cells have more complicated ways of regulating genes. Gene expression is the transcription and translation of genes into proteins. Some genes have pro ...
... repressor turns the operator off by binding to it. This process enables prokaryotes to match their cell chemistry to different conditions. Eukaryotic cells have more complicated ways of regulating genes. Gene expression is the transcription and translation of genes into proteins. Some genes have pro ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.