• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
DNA and Protein Synthesis
DNA and Protein Synthesis

Dioxyribose Nucleic Acid
Dioxyribose Nucleic Acid

... MRNA – Messenger RNA (mRNA) carries the amino acid code from the nucleus to the ribosomes in the cytoplasm. – When the ribosomes get the code, they can start making proteins. ...
ANSWERS TO REVIEW QUESTIONS
ANSWERS TO REVIEW QUESTIONS

... heredity, and applies to all species. Sequencing the human genome applies only to us and has so far helped researchers more than it has led to treatments. 6. The film GATTACA depicts a society based on knowing genome sequences. Crime television shows such as the Law and Order and CSI programs regula ...
BioSc 231 Exam 5 2008
BioSc 231 Exam 5 2008

... Read the fragments on the gel from top to bottom, in order of increasing size. Read the fragments on the gel from bottom to top, in order of increasing size. Read each lane individually from top to bottom and pool the results. All of the above are legitimate approaches. None of the above ...
BACKGROUND INFORMATION:
BACKGROUND INFORMATION:

... of the molecules at that sequence. The result is a set of double-stranded DNA fragments with singlestranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the single stranded ends do easily form base pairs with the complementary bases on other DNA molecules. Bec ...
Chapter 11: DNA
Chapter 11: DNA

... In the beginning… • Is the genetic material in cells protein or DNA? • In order to be the genetic material, it must be… 1. Able to store information that pertains to the development, structure and metabolic activities of the cell 2. Stable so that it can be replicated 3. Able to undergo changes (mu ...
DNA
DNA

... • DNA is found in the mitochondria. • mDNA is only found in the egg. Sperm  has no mitochondria so mDNA is passed  to offspring from the mother. • One sequence of DNA is a genome or  gene. • Unwind all our DNA, it will stretch from the moon  and back 6000X. ...
1 BIOL 213 Fourth Exam All atoms, chemical bonding and structures
1 BIOL 213 Fourth Exam All atoms, chemical bonding and structures

... A mutation in the gene for ferredoxin occurs such that ferredoxin cannot pass its electrons to FAD yet is functional otherwise. The photosynthetic organism is still able to continue growth. Explain. ...
File
File

... b. Human body is composed of trillions of cells. c. Cells provide structure for a body, take in nutrients from food, convert nutrients into energy, and carry out specialised functions. ...
Promoter Analysis
Promoter Analysis

... depends on co-factors • E.g. ER sits on the DNA but requires estrogen as a co-factor to function • Myc requires Max as a cofactor to stimulate transcription • If Max is coupled with Mad instead, the genes are repressed ...
BIO I Review Packet Protein Synthesis 2017
BIO I Review Packet Protein Synthesis 2017

... 18. What are the three forms of RNA, what do they stand for, and what is their function? ...
Biotechnology
Biotechnology

... genetic engineering) of living organisms or their components to produce useful commercial products  Recombinant DNA (rDNA) technology: ...
DNA Structure and Function Notes
DNA Structure and Function Notes

... Since then, scientists have discovered that genes are the instructions for inherited traits. ...
CH 16 PPT
CH 16 PPT

... Chapter 16 The Molecular Basis of Inheritance ...
Practice Quizzes for Honors Biology Unit 3
Practice Quizzes for Honors Biology Unit 3

... Chapter  26:  Control  of  Gene  Expression  and  Cancer   1. How  do  cells  become  specialized  when  they  all  contain  the  exact  same  DNA?   2. For  the  operon;  name  the  participant  that:   a. transcribes  the  DNA  into   ...
Genetic Engineering and Biotechnology
Genetic Engineering and Biotechnology

... Stem Cell Research (we will come back to this one later) Gene Therapy DNA Fingerprinting (and other Forensics applications) ...
Dna And Forensics Essay Research Paper DNA
Dna And Forensics Essay Research Paper DNA

HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA
HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA

... of circular ssDNA. The plasmid contains SP6 and T7 RNA polymerase promoters flanking the multiple cloning sites. The multiple cloning site (or polylinker) includes unique restriction sites for Apa I, Aat II, Sph I, Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Hind III, BamH I, Sac I, BstX I a ...
BioSc 231 Exam 5 2003
BioSc 231 Exam 5 2003

... C. identification of a gene D. a restriction fragment length polymorphism _____ Which of the following is NOT necessary in order for a population to maintain Hardy-Weinberg equilibrium? A. random mating B. high rate of migration C. large population size D. allele frequencies are the same in males an ...
Big slides
Big slides

... Biological Diversity • Many different types of Life on earth • All with different morphologies…. ...
Lecture 9
Lecture 9

Aim: What are some techniques used in DNA engineering?
Aim: What are some techniques used in DNA engineering?

... DNA from tiny amount of blood or semen found at the scenes of violent crimes, DNA from single embryonic cells for rapid prenatal diagnosis of genetic disorders, DNA of viral genes from cells infected with difficult-to-detect viruses such as HIV. ...
Exp 4 Lecture - Seattle Central College
Exp 4 Lecture - Seattle Central College

... • Genetic transformation involves the insertion of some new DNA into the E. coli cells. In addition to one large chromosome, bacteria often contain one or more small circular pieces of DNA called plasmids. • Plasmid DNA usually contains genes for more than one trait. Genetic engineering involves ins ...
DNA Barcoding
DNA Barcoding

... All eukaryotes contain mitochondria; COI encodes a mitochondrial protein needed for cells to make ATP. COI is almost identical within a species but varies between different species. Agreement among scientists that the COI gene is used for animal barcoding. ...
Systematic Implications of DNA variation in subfamily
Systematic Implications of DNA variation in subfamily

... Early methods relied on chromatography to separate complex mixtures of secondary metabolites, detect them, and then compare between taxa “spot botanists” – very phenetic Better separation and identification methods developed – used pathway stages as cladistic characters - phytochemistry Move away fr ...
< 1 ... 584 585 586 587 588 589 590 591 592 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report