Altering substrate specificity of catechol 2,3
... C23O for tested catecholic compounds is shown in Table 1. The lowest activity towards catechol and catechols with substituent in para-position (4-chlorocatechol, 4-methylcatechol) was found for mutant C23OB58 (Table 1). In this mutant three amino acid changes were localized: H24R, F168S, Q275R. Base ...
... C23O for tested catecholic compounds is shown in Table 1. The lowest activity towards catechol and catechols with substituent in para-position (4-chlorocatechol, 4-methylcatechol) was found for mutant C23OB58 (Table 1). In this mutant three amino acid changes were localized: H24R, F168S, Q275R. Base ...
13059_2010_2366_MOESM1_ESM
... The final P. ultimum DAOM BR144 genome assembly used for analysis consisted of 975 scaffolds that were comprised of 1,747 contigs with an average depth of coverage of ~8X. The N50 contig length is 124 kb and the N50 scaffold length is 773,464 bp. There are 772 scaffold gaps comprising 2.1Mb of N’s i ...
... The final P. ultimum DAOM BR144 genome assembly used for analysis consisted of 975 scaffolds that were comprised of 1,747 contigs with an average depth of coverage of ~8X. The N50 contig length is 124 kb and the N50 scaffold length is 773,464 bp. There are 772 scaffold gaps comprising 2.1Mb of N’s i ...
Cloning in bacteria other than Escherichia coli
... used. The reasons for this are threefold. First, it is relatively simple to do, particularly now that competent cells are commercially available. Secondly, it can be very efficient. Efficiencies of 108–109 transformants/ µg plasmid DNA are readily achievable and are more than adequate for most appli ...
... used. The reasons for this are threefold. First, it is relatively simple to do, particularly now that competent cells are commercially available. Secondly, it can be very efficient. Efficiencies of 108–109 transformants/ µg plasmid DNA are readily achievable and are more than adequate for most appli ...
BioInformatics at FSU
... we’ve only got about twice as many as a fruit fly, between 25,000 and 35,000! The protein coding region of our genome is only about 1% or so, much of the remainder ‘junk’ is ‘jumping,’ ‘selfish DNA’ of which much may be involved in regulation and control. Understanding this network is a huge challen ...
... we’ve only got about twice as many as a fruit fly, between 25,000 and 35,000! The protein coding region of our genome is only about 1% or so, much of the remainder ‘junk’ is ‘jumping,’ ‘selfish DNA’ of which much may be involved in regulation and control. Understanding this network is a huge challen ...
Plant Physiology
... Figure 4C. Dg93 transcripts accumulated to high levels in a band of cells proximal to the nodule meristem, some of which contained visible Frankia (Fig. 4, C and D). A region of less-intense blue can be seen below the first zone (Fig. 4, C and D). The peripheral, doughnut-shaped configuration of Fra ...
... Figure 4C. Dg93 transcripts accumulated to high levels in a band of cells proximal to the nodule meristem, some of which contained visible Frankia (Fig. 4, C and D). A region of less-intense blue can be seen below the first zone (Fig. 4, C and D). The peripheral, doughnut-shaped configuration of Fra ...
Identification of sixteen single-nucleotide polymorphism markers in
... (AS-PCR) (Newton et al. 1989), is more flexible with a reasonable efficiency and expense (Huang et al. 2005; Gaudet et al. 2007), and has also been applied the identification of SNP markers in mollusks, such as Meretrix meretrix (Li et al. 2010), Crassostrea gigas (Wang et al. 2010) and Mytilus gall ...
... (AS-PCR) (Newton et al. 1989), is more flexible with a reasonable efficiency and expense (Huang et al. 2005; Gaudet et al. 2007), and has also been applied the identification of SNP markers in mollusks, such as Meretrix meretrix (Li et al. 2010), Crassostrea gigas (Wang et al. 2010) and Mytilus gall ...
Bacterial Culturing of Sterile Body Fluids Using the VersaTREK
... Over a 5 month period we cultured 175 samples (pleural fluid = 53, hip fluid = 11, pleural fluid = 53, peritoneal fluid = 28, and pericardial fluid = 15) from 159 different patients using VersaTREK® blood culture bottles in parallel with solid media. Overall 121 (69%) of the samples were sterile by ...
... Over a 5 month period we cultured 175 samples (pleural fluid = 53, hip fluid = 11, pleural fluid = 53, peritoneal fluid = 28, and pericardial fluid = 15) from 159 different patients using VersaTREK® blood culture bottles in parallel with solid media. Overall 121 (69%) of the samples were sterile by ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
Consortia of cyanobacteria/microalgae and bacteria
... complex relationships with trophically-related bacterial groups and the physiologies of habitat-forming (edificatory) cyanobacteria. These cyanobacterial mats were credited with self-cleaning properties in the Arabian Gulf Coasts (Sorkhoh et al., 1992). ‘Symbiosis,’ which was defined as two or more di ...
... complex relationships with trophically-related bacterial groups and the physiologies of habitat-forming (edificatory) cyanobacteria. These cyanobacterial mats were credited with self-cleaning properties in the Arabian Gulf Coasts (Sorkhoh et al., 1992). ‘Symbiosis,’ which was defined as two or more di ...
A1114-CFS-SD1 - Food Standards Australia New Zealand
... Figure 1: The corn wet milling process (diagram taken from CRA (2006)) ............................. 6 Figure 2: Genes and regulatory elements contained in plasmid PV-ZMAP5714 .................... 8 Figure 3: Breeding diagram for MON87403 ............................................................. ...
... Figure 1: The corn wet milling process (diagram taken from CRA (2006)) ............................. 6 Figure 2: Genes and regulatory elements contained in plasmid PV-ZMAP5714 .................... 8 Figure 3: Breeding diagram for MON87403 ............................................................. ...
DNA sequences I I. Sequence editing
... further ML analysis of your data. The advantage of this approach is the possibility to load single dataset that can be divided into the partitions (as described above). Software then test all DNA models for all partitions and further decide, which partitions ...
... further ML analysis of your data. The advantage of this approach is the possibility to load single dataset that can be divided into the partitions (as described above). Software then test all DNA models for all partitions and further decide, which partitions ...
Nitrifyers in constructed wetlands treating landfill leachates Carina Sundberg
... by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full- ...
... by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full- ...
Initiates file download
... • Possible secondary effects from expression of the inserted gene or the disruption of host DNA or metabolic pathways, including composition of critical macro- and micronutrients, antinutrients, endogenous toxicants, allergens, and physiologically active substances. • The inherent differences betwee ...
... • Possible secondary effects from expression of the inserted gene or the disruption of host DNA or metabolic pathways, including composition of critical macro- and micronutrients, antinutrients, endogenous toxicants, allergens, and physiologically active substances. • The inherent differences betwee ...
No Evidence of Neandertal mtDNA Contribution to Early Modern
... excluded at the 5% level (Figure S3). A more realistic scenario may be that the spread of modern humans was accompanied by an increase in population size before and during their migration out of Africa and subsequent colonization of western Eurasia (see Figure 4B). In that case, the Neandertal contr ...
... excluded at the 5% level (Figure S3). A more realistic scenario may be that the spread of modern humans was accompanied by an increase in population size before and during their migration out of Africa and subsequent colonization of western Eurasia (see Figure 4B). In that case, the Neandertal contr ...
ADVANCING JUSTICE THROUGH DNA TECHNOLOGY
... DNA technology is increasingly vital to ensuring accuracy and fairness in the criminal justice system. DNA can be used to identify criminals with incredible accuracy when biological evidence exists, and DNA can be used to clear suspects and exonerate persons mistakenly accused or convicted of crimes ...
... DNA technology is increasingly vital to ensuring accuracy and fairness in the criminal justice system. DNA can be used to identify criminals with incredible accuracy when biological evidence exists, and DNA can be used to clear suspects and exonerate persons mistakenly accused or convicted of crimes ...
Clustering Method for Repeat Analysis in DNA sequences
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
PPT
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
Measuring Arsenite using E. coli reporter strains
... Under conditions during which the E. coli cells do not encounter arsenite, the ArsR protein will inhibit synthesis of the eGFP. When arsenite enters the cell, the ArsR protein will release from its binding site, similar as for the original arsenite resistance mechanism. However, in the arsenite test ...
... Under conditions during which the E. coli cells do not encounter arsenite, the ArsR protein will inhibit synthesis of the eGFP. When arsenite enters the cell, the ArsR protein will release from its binding site, similar as for the original arsenite resistance mechanism. However, in the arsenite test ...
Implementation of a Rapid Microarray Assay for the Detection of
... Gram-negative bacteria which may cause bloodstream infections. This type of technology significantly reduces the time, by approximately about 48 hours, required for pathogen identification and susceptibility testing. However, the Verigene technology is indicated in conjunction with other clinical an ...
... Gram-negative bacteria which may cause bloodstream infections. This type of technology significantly reduces the time, by approximately about 48 hours, required for pathogen identification and susceptibility testing. However, the Verigene technology is indicated in conjunction with other clinical an ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
... increased rapidly from being rare and expensive pieces of equipment used primarily by the business elite, to a common low-cost personal item. Majority of the mobile phones are hand- held. In developed countries and in many developing countries , many childrens now own a personal mobile phone. With t ...
... increased rapidly from being rare and expensive pieces of equipment used primarily by the business elite, to a common low-cost personal item. Majority of the mobile phones are hand- held. In developed countries and in many developing countries , many childrens now own a personal mobile phone. With t ...
Expression of floral identity genes in Clianthus
... late July, the size of the petals increased rapidly and further differentiated into distinct vexillum, wing and keel, with the height of the vexillum exceeding that of the carpel (Fig. 2K, L). At this stage, the height of the gynoecium still exceeded that of the androecium. By August, the volume of ...
... late July, the size of the petals increased rapidly and further differentiated into distinct vexillum, wing and keel, with the height of the vexillum exceeding that of the carpel (Fig. 2K, L). At this stage, the height of the gynoecium still exceeded that of the androecium. By August, the volume of ...
Sequences of the Nucleocapsid Genes from Two Strains of Avian
... cDNA chining. Three cDNA clones, derived from RNA isolated from gradient-purified virus, have been used for sequencing. For Beaudene the oligo(dT)-primed clone C5.322 was used, which includes the poly(A) tail derived from the 3' terminus of the viral genome and extends for 1930base pairs in the 5' d ...
... cDNA chining. Three cDNA clones, derived from RNA isolated from gradient-purified virus, have been used for sequencing. For Beaudene the oligo(dT)-primed clone C5.322 was used, which includes the poly(A) tail derived from the 3' terminus of the viral genome and extends for 1930base pairs in the 5' d ...