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Principles of Life - bli-research-synbio-2014-session-2
Principles of Life - bli-research-synbio-2014-session-2

... In-Text Art, Ch. 10, p. 195 ...
Coliform indicators - Longwood University
Coliform indicators - Longwood University

... commodities (Ashbolt et al.,2001). Although alternative bacterial indicators have been suggested (e.g., fecal streptococci, sulphite-reducing Clostridia), various forms of coliform bacteria have been used since the 1920’s as standard microbial indicators for quality assessments. The cultivation and ...
nitrogen transformations
nitrogen transformations

... The key to understanding mineralization–immobilization is to “think like a microbe” that is attempting to make a living by obtaining energy and C from detritus. Sometimes the detritus has all the N that the microbe needs, so as C is consumed, any extra N is released (mineralized) to the soil solutio ...
- Waynesburg University
- Waynesburg University

... carries  the  bacteria  away.  As  a  result,  it  is  possible  to  get  sick  from  drinking  from  a  stream  or  other  body  of  water  near  farmland.  Contamination  can  also  come  from  areas  where  the  ...
Next Step Bio Supplement
Next Step Bio Supplement

... recognize different promoter regions. For example, E. coli can produce seven different factors that initiate transcription of a factor-specific subset of genes. Some sigma factors, distinguished from one another by their molecular weight, bind to the promoters of genes that encode proteins or RNA mo ...
AZT resistance of simian foamy virus reverse transcriptase is based
AZT resistance of simian foamy virus reverse transcriptase is based

... We then used the heteropolymeric single-stranded M13 substrate with a 32P-endlabeled DNA-primer for polymerization in the absence of inhibitor or in the presence of 5 and 50 mM AZTTP and analyzed the polymerization products on denaturing polyacrylamide gels (Figure 2B). As already described for the ...
12–4 Mutations - Gravette School District
12–4 Mutations - Gravette School District

... genetic variability in a species. Some of this variation may be highly beneficial. Mutations are the causes of many genetic disorders, including sickle cell disease and cystic fibrosis, both discussed in Chapter 14. Harmful mutations are also associated with many types of cancer. In contrast, benefi ...
PROBIOTICS IN YELLOW PERCH AND TILAPIA CULTURE
PROBIOTICS IN YELLOW PERCH AND TILAPIA CULTURE

Narcissus pseudonarcissus L. `Dutch Master`
Narcissus pseudonarcissus L. `Dutch Master`

... Megalign, and SeqMan programs (DNASTAR, Madison, WI). Sequences were compared to sequences in the GenBank database using the BLASTX algorithm [22]. If the sequence was registered as an unknown protein, but had a conserved domain, the sequence was labeled according to its conserved domain. Because th ...
economic perspectives
economic perspectives

... of genetically engineered organisms in food and agriculture have illustrated that this boundary is not so clear. Not only are there safety concerns about genetically engineered organisms, but there are also cultural differences in acceptance of the products. International contexts for technologies a ...
A phylogenomic study of DNA repair genes, proteins
A phylogenomic study of DNA repair genes, proteins

Sequencing genomes
Sequencing genomes

... Consider a result with an E = 1. This value indicates that in a database of this particular size one match with a similar score is expected to occur by chance. If the database were twice as big, there would be twice the likelihood of finding a score equal to or greater than S by chance. ...
Amino Acid Analysis Quick Reference Card For Hydrolysate
Amino Acid Analysis Quick Reference Card For Hydrolysate

... This document supports amino acid analysis for hydrolysate samples using iTRAQ™ Reagents and the AB SCIEX Amino Acid 20/20 Analyzer. The labeling protocol labels a peptide hydrolysate, protein hydrolysate, or a hydrolysate from animal feed sample (dry ≈ 10 nmol amino acid) with iTRAQ Reagent 117. An ...
Number 53, 2006 5 m sh4
Number 53, 2006 5 m sh4

... bioinformatic analysis used tBlastn to identify a region with homology to MSH4 sequences of S. cerevisiae, Mouse and Human (Borkovich et al., 2004). Alignment of the predicted protein sequence with human and S. cerevisiae MSH4 protein sequences using ClustalW showed that Neurospora MSH4 shares conse ...
Sequencing genomes
Sequencing genomes

... Consider a result with an E = 1. This value indicates that in a database of this particular size one match with a similar score is expected to occur by chance. If the database were twice as big, there would be twice the likelihood of finding a score equal to or greater than S by chance. ...
DNA-Directed Antibody Immobilization for
DNA-Directed Antibody Immobilization for

... piezoelectric arrayer. All antibodies were spotted in PBS with 50 mM Trehalose. All ssDNA surface probes were spotted at a concentration of 30 μM in sodium phosphate buffer (150 mM, pH = 8.5). The spotted chips were kept overnight in the spotter chamber at 67% humidity. Following the overnight immobi ...
Course description - Faculty Members Websites
Course description - Faculty Members Websites

... introduced to the light microscopy and electronic microscopy. A.3 To get introduced to the common types of microbial stains, and the functions and results of each steps in the Gram staining procedure A.4 know the dichotomous key used for naming microorganisms, and the significance of Bergey’s manual ...
11.7 Repressor binds cooperatively at each operator using a helix
11.7 Repressor binds cooperatively at each operator using a helix

... large number of phage particles, lysis of the cell, and release of the viruses. 2. Lytic infection falls typically into three phases. In the first phase a small number of phage genes are transcribed by the host RNA polymerase. 3. In phage lambda, the genes are organized into groups whose expression ...
BMC Evolutionary Biology D- and L-lactate dehydrogenases during invertebrate evolution
BMC Evolutionary Biology D- and L-lactate dehydrogenases during invertebrate evolution

... enzyme. This hypothesis emerged from phylogenetic reconstructions and from the observation that the single LDH isozyme of the primitive agnathan, the sea lamprey, is immunologically more similar to LDH-C in teleost fish than to LDH-A or LDH-B [14-16]. Our phylogenetic analyses of L-LDH isozymes are ...
British Journal of Dairy Sciences 3(2): 9-13, 2013
British Journal of Dairy Sciences 3(2): 9-13, 2013

... Bovine based milk and milk products represent a good source of protein (Korhonen et al., 1998; Clare and Swaisgood, 2000), lipids (Jensen and Newburg, 1995), amino acids (Nilson et al., 2007), vitamins (Hayes et al., 2001; Forssen et al., 2000) and minerals (Brain, 2008). The nutritional benefits of ...
Complete Protocol
Complete Protocol

... The FluoroTect™ GreenLys in vitro Translation Labeling System(a) allows fluorescent labeling of in vitro translation products through the use of a modified charged lysine transfer RNA labeled with the fluorophore BODIPY®-FL. Using this system, fluorescently labeled lysine residues are incorporated into ...
Characterization of Acetyl-CoA Carboxylases in the Basal
Characterization of Acetyl-CoA Carboxylases in the Basal

... over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed  over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed near  the  light:dark  transition  [22,23].  The  ACC  transcripts  closely  followed  this  patter ...
P.abyssi PDF version
P.abyssi PDF version

... These DNA rearrangements do not correlate with replication but are instead confined to one replichore (Zivanovic et al., 2002). Other rearrangements correspond to the mobility of long clusters of repeated sequences that could play a role in chromosome segregation (Mojica et al., 2000; Zivanovic et a ...
Altering substrate specificity of catechol 2,3
Altering substrate specificity of catechol 2,3

... C23O for tested catecholic compounds is shown in Table 1. The lowest activity towards catechol and catechols with substituent in para-position (4-chlorocatechol, 4-methylcatechol) was found for mutant C23OB58 (Table 1). In this mutant three amino acid changes were localized: H24R, F168S, Q275R. Base ...
Optical Tweezers: Measuring Piconewton Forces
Optical Tweezers: Measuring Piconewton Forces

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