Isolation of a UV Endonuclease from the
... ( 0 - 0 2 M), NaCl (0.1 M), MgSO, ( l o F 3M) and gelatin (0.01 %, w/v) was lysed by adding 1 ml chloroform and shaking the suspension for 10 rnin at 37 "C.Bovine pancreatic DNAase (10 pl) (Sigma) at a concentration of 1 mg ml-I in water was added and shaken slowly for 10 rnin at 37 OC. The DNAase t ...
... ( 0 - 0 2 M), NaCl (0.1 M), MgSO, ( l o F 3M) and gelatin (0.01 %, w/v) was lysed by adding 1 ml chloroform and shaking the suspension for 10 rnin at 37 "C.Bovine pancreatic DNAase (10 pl) (Sigma) at a concentration of 1 mg ml-I in water was added and shaken slowly for 10 rnin at 37 OC. The DNAase t ...
Biotechnology and Agrobiodiversity
... Processing). However, as a result of the recent advances in molecular biology and genetics, existing biotechnological applications have been refined and many new biotechnological tools have been developed. Modern biotechnology has left the laboratory and reached the market place. The impact of biote ...
... Processing). However, as a result of the recent advances in molecular biology and genetics, existing biotechnological applications have been refined and many new biotechnological tools have been developed. Modern biotechnology has left the laboratory and reached the market place. The impact of biote ...
Hybrid Antibiotics
... The gene for 11-hydroxylase from the doxorubicin producer Streptomyces peuceticus ATCC 27952 was introduced into the aklavinone producer Streptomyces galilaeus ATCC 31133. The transformed strain produced a number of red colored metabolites such as 11hydroxyaclacinomycin A, B, T and X (Fig. 15). 11-H ...
... The gene for 11-hydroxylase from the doxorubicin producer Streptomyces peuceticus ATCC 27952 was introduced into the aklavinone producer Streptomyces galilaeus ATCC 31133. The transformed strain produced a number of red colored metabolites such as 11hydroxyaclacinomycin A, B, T and X (Fig. 15). 11-H ...
Genome-sequence analysis of Acinetobacter johnsonii MB44
... content of A. johnsonii MB44 was higher compared with those of A. baumannii AB307-0294 and A. pittii ANC 4052. Previously, ANI value between A. johnsonii MB44 and A. johnsonii ATCC 17909T was calculated and it was found as 95.65 % (Tian et al. 2016). Furthermore, the ANI value between A. johnsonii ...
... content of A. johnsonii MB44 was higher compared with those of A. baumannii AB307-0294 and A. pittii ANC 4052. Previously, ANI value between A. johnsonii MB44 and A. johnsonii ATCC 17909T was calculated and it was found as 95.65 % (Tian et al. 2016). Furthermore, the ANI value between A. johnsonii ...
powerpoint
... DNA Sequencing • Relatively short DNA fragments can be sequenced by the dideoxy chain termination method, the first automated method to be employed • Modified nucleotides called dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with ...
... DNA Sequencing • Relatively short DNA fragments can be sequenced by the dideoxy chain termination method, the first automated method to be employed • Modified nucleotides called dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with ...
Biology
... 27. (1pt) The “guardian angel gene” codes for a protein that will destroy the cell that it is in, if it turns cancerous. If the mutation in the previous question occurs in p53, how many of the cells resulting from mitosis of this cell will carry the mutation? ...
... 27. (1pt) The “guardian angel gene” codes for a protein that will destroy the cell that it is in, if it turns cancerous. If the mutation in the previous question occurs in p53, how many of the cells resulting from mitosis of this cell will carry the mutation? ...
Chromocurvus halotolerans gen. nov., sp. nov., a
... added singly at a concentration of 1 g/l: acetate, bactopeptone, butyrate, casamino acids, citrate, ethanol, formate, fructose, glucose, glutamate, glycerol, glycolate, lactate, malate, methanol, propionate, pyruvate, succinate, sucrose, and yeast extract. Autotrophy was tested by measuring growth i ...
... added singly at a concentration of 1 g/l: acetate, bactopeptone, butyrate, casamino acids, citrate, ethanol, formate, fructose, glucose, glutamate, glycerol, glycolate, lactate, malate, methanol, propionate, pyruvate, succinate, sucrose, and yeast extract. Autotrophy was tested by measuring growth i ...
Using virus-induced gene silencing to identify novel regulators of
... A commonly used virus for VIGS is TRV. TRV is an effective virus for VIGS because the symptoms of infection are mild, the virus often infects large areas, and this particular virus can be used for silencing in growing plants, reducing the amount of time needed for host growth (9). TRV is a bipartite ...
... A commonly used virus for VIGS is TRV. TRV is an effective virus for VIGS because the symptoms of infection are mild, the virus often infects large areas, and this particular virus can be used for silencing in growing plants, reducing the amount of time needed for host growth (9). TRV is a bipartite ...
MPA Monitoring Metrics: Kelp and Shallow Rock Ecosystems (0
... Monitoring metrics for the Kelp and Shallow Rock Ecosystem (0-30M) Feature in the South Coast region are provided on the next page. A summary list of the monitoring metrics is provided, including the metrics for the Ecosystem Feature Checkup (orange) and Assessment (green) options. Additional inform ...
... Monitoring metrics for the Kelp and Shallow Rock Ecosystem (0-30M) Feature in the South Coast region are provided on the next page. A summary list of the monitoring metrics is provided, including the metrics for the Ecosystem Feature Checkup (orange) and Assessment (green) options. Additional inform ...
Influence of fermentable carbohydrates or protein on large intestinal
... diets containing high fCP. Dietary inclusion of fCHO reduced for the abundance of some clostridia, amines, and NH3 in the colon associated with high fCP but did not affect the mucosal response (Pieper et al., 2012). Analysis of metabolomic profiles from colon contents and urine revealed a distinct c ...
... diets containing high fCP. Dietary inclusion of fCHO reduced for the abundance of some clostridia, amines, and NH3 in the colon associated with high fCP but did not affect the mucosal response (Pieper et al., 2012). Analysis of metabolomic profiles from colon contents and urine revealed a distinct c ...
European workshop on Phenotype MicroArrays
... The title of this year’s event “Florence Conference on Phenotype MicroArray Analysis of Cells” reflects our aspiration to provide an opportunity for researchers working in different fields of biology to share, compare and evaluate multiple types of Phenotype MicroArray data, and to show the numerous ...
... The title of this year’s event “Florence Conference on Phenotype MicroArray Analysis of Cells” reflects our aspiration to provide an opportunity for researchers working in different fields of biology to share, compare and evaluate multiple types of Phenotype MicroArray data, and to show the numerous ...
吴冬茵
... Two methods are used to evaluate the experimental results: the receiver operating characteristic (ROC) scores. the median rate of false positives (M-RFP) scores. The fraction of negative test sequences that score as high or better than the median score of the positive sequences. ...
... Two methods are used to evaluate the experimental results: the receiver operating characteristic (ROC) scores. the median rate of false positives (M-RFP) scores. The fraction of negative test sequences that score as high or better than the median score of the positive sequences. ...
Classification Chapter 18 - Forest Hills School District
... All of the classification methods discussed so far are based on physical similarities and differences. Even organisms with very different anatomies can share common traits. EX: All living things use ______________to pass on DNA and RNA information and control growth. http://sbchem.sunysb.edu/msl/dn ...
... All of the classification methods discussed so far are based on physical similarities and differences. Even organisms with very different anatomies can share common traits. EX: All living things use ______________to pass on DNA and RNA information and control growth. http://sbchem.sunysb.edu/msl/dn ...
Biochemical Defects Associated with Cancer
... Loss of mismatch repair (MMR) is linked to significantly increased cancer risk in a condition known as Lynch Syndrome. This disorder is characterized by a predisposition to early onset of colorectal and other internal cancers. Knowing who is at risk for cancer because of MMR deficiency can influence ...
... Loss of mismatch repair (MMR) is linked to significantly increased cancer risk in a condition known as Lynch Syndrome. This disorder is characterized by a predisposition to early onset of colorectal and other internal cancers. Knowing who is at risk for cancer because of MMR deficiency can influence ...
Avian Infectious Bronchitis Virus (IBV)
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
Clostridium hiranonis sp. nov., a human intestinal bacterium with
... JCM 3814T and C. glycolicum JCM 1401T were used as the reference strains because they were closely related to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 we ...
... JCM 3814T and C. glycolicum JCM 1401T were used as the reference strains because they were closely related to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 we ...
NIHMS88703-supplement-2
... Overlap between liver signature genes from mouse models and a macrophage-enriched metabolic network (MEMN) Based on the co-expression networks constructed from liver and adipose tissues collected from a mouse cross between C57BL/6J (B6) and C3H/HeJ on an apolipoprotein E null background (BXH/apoE), ...
... Overlap between liver signature genes from mouse models and a macrophage-enriched metabolic network (MEMN) Based on the co-expression networks constructed from liver and adipose tissues collected from a mouse cross between C57BL/6J (B6) and C3H/HeJ on an apolipoprotein E null background (BXH/apoE), ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Word - The Open University
... Plasmids are small circular pieces of DNA found in bacteria. They are from 10 to 1000 times smaller than the main circular DNA loop (bacterial chromosome) which forms the genomic DNA. ...
... Plasmids are small circular pieces of DNA found in bacteria. They are from 10 to 1000 times smaller than the main circular DNA loop (bacterial chromosome) which forms the genomic DNA. ...
Bioinformatics Assignment
... Each sequence has a score (bits) , which tells you how closely aligned the 2 sequences are. By clicking on the Score, it will take you down to a match. Each match appears as follows: Query: 1 Sbjct: 300 (or some other number) Query is the sequence you submitted for analysis. Sbjct is the match that ...
... Each sequence has a score (bits) , which tells you how closely aligned the 2 sequences are. By clicking on the Score, it will take you down to a match. Each match appears as follows: Query: 1 Sbjct: 300 (or some other number) Query is the sequence you submitted for analysis. Sbjct is the match that ...