Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes
... provided by R. Mauer and C. Miller. Plasmids were mobilized by bacteriophage P22 transduction as described previously (35). All transduction recipients used in initial clone identification carried three mutations: a recA mutation was included to prevent recombination, a metE mutation rendered methio ...
... provided by R. Mauer and C. Miller. Plasmids were mobilized by bacteriophage P22 transduction as described previously (35). All transduction recipients used in initial clone identification carried three mutations: a recA mutation was included to prevent recombination, a metE mutation rendered methio ...
the function and synthesis of ribosomes
... solved by X-ray crystallography with atomic resolution1–4. The analyses used ribosomes from the thermophilic bacterium Thermus thermophilus for the 30S subunit and the intact 70S ribosome, and the archaeon Haloarcula marismortui for the 50S subunit. Structural analyses followed and built on electron ...
... solved by X-ray crystallography with atomic resolution1–4. The analyses used ribosomes from the thermophilic bacterium Thermus thermophilus for the 30S subunit and the intact 70S ribosome, and the archaeon Haloarcula marismortui for the 50S subunit. Structural analyses followed and built on electron ...
Cut, Print: Our Emerging Understanding of Alternative Splicing
... different proteins (Black 2000). Though only 49 of the Drosophila DSCAM exon combinations have been observed thus far, this example illustrates the sheer capacity for proteomic dynamism from a single alternatively spliced gene. ...
... different proteins (Black 2000). Though only 49 of the Drosophila DSCAM exon combinations have been observed thus far, this example illustrates the sheer capacity for proteomic dynamism from a single alternatively spliced gene. ...
Darwin Core Standards and Beyond. Making Data and Media
... Locality: Provide a descriptive locality, even if you have geographic coordinates. Write the description from specific to general, including a specific locality, offset(s) from a reference point, and administrative units such as county, state, and country. The locality should be as specific, succinc ...
... Locality: Provide a descriptive locality, even if you have geographic coordinates. Write the description from specific to general, including a specific locality, offset(s) from a reference point, and administrative units such as county, state, and country. The locality should be as specific, succinc ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
From writing to reading the encyclopedia of life
... pseudogenes [33] or from bacterial and fungal endosymbionts [34]. Pseudogenes have proven an infrequent problem because they are typically shorter [35] and are present in lower copy numbers than the barcode regions targeted for analysis. Sequences from bacterial endosymbionts are encountered more co ...
... pseudogenes [33] or from bacterial and fungal endosymbionts [34]. Pseudogenes have proven an infrequent problem because they are typically shorter [35] and are present in lower copy numbers than the barcode regions targeted for analysis. Sequences from bacterial endosymbionts are encountered more co ...
document
... range of host cells in Enterococcus and other bacteria). Large family found in these Gram positive bacteria with broad host range. Carry Integration / excision determinants and plasmid transfer genes. INTEGRATE EXCISE -TRANSFER ON PLASMID (not covered in detail here). ...
... range of host cells in Enterococcus and other bacteria). Large family found in these Gram positive bacteria with broad host range. Carry Integration / excision determinants and plasmid transfer genes. INTEGRATE EXCISE -TRANSFER ON PLASMID (not covered in detail here). ...
- Wiley Online Library
... represents the dynamics of the net direct effects of the microbial community on individual plant types. Expected dynamics of feedback.—The growth consequences of soil community feedback can be positive or negative. For example, if the presence of plant species A causes an accumulation of microbes th ...
... represents the dynamics of the net direct effects of the microbial community on individual plant types. Expected dynamics of feedback.—The growth consequences of soil community feedback can be positive or negative. For example, if the presence of plant species A causes an accumulation of microbes th ...
Full Text - Journal of Pharmaceutical, Chemical and
... was isolated from G. biloba leaves by PCR technology. The open reading frame (ORF) of GbANR was 1023 bp, encoding 340 amino acids, and the molecular weight was 37.38 kDa, with the isoelectric point of 5.87. Bioinformatics analysis showed that the deduced GbANR shared more than 50% identity with ANR ...
... was isolated from G. biloba leaves by PCR technology. The open reading frame (ORF) of GbANR was 1023 bp, encoding 340 amino acids, and the molecular weight was 37.38 kDa, with the isoelectric point of 5.87. Bioinformatics analysis showed that the deduced GbANR shared more than 50% identity with ANR ...
Compiling DNA strand displacement reactions using a functional
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
Protocol for RiboShredder™ RNase Blend
... RiboShredder™ RNase Blend is a proprietary blend of potent RNases that completely degrade unwanted RNA in DNA purification procedures. Unlike other RNase cocktails, RiboShredder RNase Blend completely degrades all RNA. RiboShredder RNase Blend uses recombinant, highly purified ribonucleases and thus ...
... RiboShredder™ RNase Blend is a proprietary blend of potent RNases that completely degrade unwanted RNA in DNA purification procedures. Unlike other RNase cocktails, RiboShredder RNase Blend completely degrades all RNA. RiboShredder RNase Blend uses recombinant, highly purified ribonucleases and thus ...
Bacteria in Permafrost
... However, determination of phylogenetic diversity of a bacterial community from soil DNA started by Zhou et al. (1997) has come only now to the active phase. In that study, no dominant clones were found; all 43 environmental clones were different with most of the phylotypes from Proteobacteria (60.5% ...
... However, determination of phylogenetic diversity of a bacterial community from soil DNA started by Zhou et al. (1997) has come only now to the active phase. In that study, no dominant clones were found; all 43 environmental clones were different with most of the phylotypes from Proteobacteria (60.5% ...
620 024. B.Sc. Microbiology Course Structure under CBCS
... The passing minimum for CIA shall be 40% out of 25 marks [i.e. 10 marks] The passing minimum for University Examinations shall be 40% out of 75 marks [ i.e. 30 marks] ...
... The passing minimum for CIA shall be 40% out of 25 marks [i.e. 10 marks] The passing minimum for University Examinations shall be 40% out of 75 marks [ i.e. 30 marks] ...
Sequence, expression, and characterization of the first archaeal ATP
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
IV RNA Synthesis: Transcription
... increase in the DNA. This means not only that a large amount of amplified DNA can be produced in just a few hours, but that only a few molecules of target DNA need be present in the sample to start the reaction. The reaction is so specific that, with primers of 15 or so nucleotides and high annealing ...
... increase in the DNA. This means not only that a large amount of amplified DNA can be produced in just a few hours, but that only a few molecules of target DNA need be present in the sample to start the reaction. The reaction is so specific that, with primers of 15 or so nucleotides and high annealing ...
Evolutionary consequences of restricted gene flow among natural
... of crosses were obtained: (1) within-population (control) crosses, where both male and female were taken from the same population; (2) within-region crosses, where the male was taken from one and the female taken from the other population within either the central or the southern California region; ...
... of crosses were obtained: (1) within-population (control) crosses, where both male and female were taken from the same population; (2) within-region crosses, where the male was taken from one and the female taken from the other population within either the central or the southern California region; ...
Salman Razzaque - The Gene Patent Problem: Recommendations for Congress
... Another problem of patents in academic research is that it hinders the basic characteristics of research. Namely, scientific objectivity and peer review are undermined or eliminated with gene patents (Barton 2009). Scientific research has been described as a linear process where several different re ...
... Another problem of patents in academic research is that it hinders the basic characteristics of research. Namely, scientific objectivity and peer review are undermined or eliminated with gene patents (Barton 2009). Scientific research has been described as a linear process where several different re ...
RNA polymerase II
... a monocistronic message. That is, it is transcribed from a single gene and codes for only a single protein. – The word cistron is another name for a gene. ...
... a monocistronic message. That is, it is transcribed from a single gene and codes for only a single protein. – The word cistron is another name for a gene. ...
Ecological functioning of bacterial chitinases in soil - NIOO-KNAW
... as the chitin has an equivalent structure. I also tested the chiA gene diversity and abundance, as well as the dynamics of the chitinolytic bacterial community in response to the dynamics of the saprotrophic fungal biomass in the potato rhizosphere in order to test the function of bacterial chitinas ...
... as the chitin has an equivalent structure. I also tested the chiA gene diversity and abundance, as well as the dynamics of the chitinolytic bacterial community in response to the dynamics of the saprotrophic fungal biomass in the potato rhizosphere in order to test the function of bacterial chitinas ...
RNA Isolation and Technology Applications
... Microarrays for Gene Expression Profiling • Microarrays enable researchers to examine the expression levels of thousands of genes in a single experiment • As many as 50,000 or more unique DNA sequences are spotted on to a glass slide – Each spot represents one unique, known gene sequence ...
... Microarrays for Gene Expression Profiling • Microarrays enable researchers to examine the expression levels of thousands of genes in a single experiment • As many as 50,000 or more unique DNA sequences are spotted on to a glass slide – Each spot represents one unique, known gene sequence ...
Document
... Now not all genes are going to be silenced for the life of the cell/organism… Ex. The genes coding for enzymes that make glycogen in the liver… If the blood glucose concentration is low, the liver will be releasing glucose, not building glycogen from it. Therefore, the genes should be off. Likewise ...
... Now not all genes are going to be silenced for the life of the cell/organism… Ex. The genes coding for enzymes that make glycogen in the liver… If the blood glucose concentration is low, the liver will be releasing glucose, not building glycogen from it. Therefore, the genes should be off. Likewise ...
April Fools Paper slide
... Single nucleotide mismatches between the crRNA and target sequence abolishes DNA cleavage • Cleavage efficiency was tested with an array of crRNAs with a single base mismatch from the target • Mismatches up to 11 bp 5’ of the PAM site abolished cleavage • Mismatches farther upstream retained effici ...
... Single nucleotide mismatches between the crRNA and target sequence abolishes DNA cleavage • Cleavage efficiency was tested with an array of crRNAs with a single base mismatch from the target • Mismatches up to 11 bp 5’ of the PAM site abolished cleavage • Mismatches farther upstream retained effici ...
Multistep Small-Molecule Synthesis Programmed by
... purification and isolation of intermediate products, yet common methods used to purify reaction products are not suitable for synthesis on the molecular biology (picograms to micrograms) scale. To address the first of these challenges, we implemented three distinct strategies for linking chemical re ...
... purification and isolation of intermediate products, yet common methods used to purify reaction products are not suitable for synthesis on the molecular biology (picograms to micrograms) scale. To address the first of these challenges, we implemented three distinct strategies for linking chemical re ...