Down-regulation of acetolactate synthase compromises Ol
... allele in the resistant genotype encodes an isoform of the enzyme with altered kinetic properties compared with the isoform in susceptible genotypes. This altered SHMT enzyme is likely associated with perturbation of the folate pathway resulting in nutritional deficiency for nematodes. Overexpressio ...
... allele in the resistant genotype encodes an isoform of the enzyme with altered kinetic properties compared with the isoform in susceptible genotypes. This altered SHMT enzyme is likely associated with perturbation of the folate pathway resulting in nutritional deficiency for nematodes. Overexpressio ...
BioMEDIA ASSOCIATES LLC HIDDEN BIODIVERSITY Series Feeding of microorganisms
... feed on organic substances previously produced by some other organism, either autotrophic or heterotrophic, and therefore, they have adopted certain strategies to capture their food. ...
... feed on organic substances previously produced by some other organism, either autotrophic or heterotrophic, and therefore, they have adopted certain strategies to capture their food. ...
Quick Overview of Bioinformatics - What's New?
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
Metabolic Interactions of aPurple Sulfur Bacterium and a Facultative
... transmission electron microscopy, the cells have a single polar flagellum. Spectral analysis of PSBI revealed peaks at 590, 800, and 850 nm, all of which are indicative of bacteriochlorophyll a. PSBI rapidly utilized sulfide, and sulfide depl eted cultures (no internal granules) ...
... transmission electron microscopy, the cells have a single polar flagellum. Spectral analysis of PSBI revealed peaks at 590, 800, and 850 nm, all of which are indicative of bacteriochlorophyll a. PSBI rapidly utilized sulfide, and sulfide depl eted cultures (no internal granules) ...
Detailed Supporting Information
... SI Text 4: Intracellular flux measurements Glycogen glucose and RNA ribose stable isotope studies: RNA ribose and glycogen glucose were isolated by acid hydrolysis of cellular RNA after Trizol purification of cell extracts. Total RNA amounts were assessed by spectrophotometric determination, in trip ...
... SI Text 4: Intracellular flux measurements Glycogen glucose and RNA ribose stable isotope studies: RNA ribose and glycogen glucose were isolated by acid hydrolysis of cellular RNA after Trizol purification of cell extracts. Total RNA amounts were assessed by spectrophotometric determination, in trip ...
NucleoSpin 96 Flash Plasmid and Large-Construct DNA
... medium (with appropriate antibiotic; for example 100 μg/mL ampicillin) to each well of the Square-well Block. To avoid cross-contamination due to spillage during incubation, do not exceed a total culture volume of 1.5 mL. Inoculate each well with a single bacterial colony. Cover the Square-well Bloc ...
... medium (with appropriate antibiotic; for example 100 μg/mL ampicillin) to each well of the Square-well Block. To avoid cross-contamination due to spillage during incubation, do not exceed a total culture volume of 1.5 mL. Inoculate each well with a single bacterial colony. Cover the Square-well Bloc ...
(Heterobasidion annosum) in
... pressed sequence tags (ESTs) was performed at three stages (1, 5 and 15 days post inoculation; d.p.i.) during pathogen development corresponding to adhesion/hyphal growth, cortical invasion and vascular colonization, respectively, with corresponding host responses in the form of recognition, active ...
... pressed sequence tags (ESTs) was performed at three stages (1, 5 and 15 days post inoculation; d.p.i.) during pathogen development corresponding to adhesion/hyphal growth, cortical invasion and vascular colonization, respectively, with corresponding host responses in the form of recognition, active ...
the role of earthworm gut-associated microorganisms
... The group of prion diseases also known as transmissible spongiform encephalopathy (TSE) includes the most well-known human prion diseases (kuru, Creutzfeldt-Jakob disease (CJD), and its variants (familial, sporadic, latrogenic, fatal familial insomnia and the new variant of CJD (vCJD) (Will et al. ( ...
... The group of prion diseases also known as transmissible spongiform encephalopathy (TSE) includes the most well-known human prion diseases (kuru, Creutzfeldt-Jakob disease (CJD), and its variants (familial, sporadic, latrogenic, fatal familial insomnia and the new variant of CJD (vCJD) (Will et al. ( ...
1 Rhizosphere Biology – an Overview
... microbes and their ratio. The chemical and physical nature of the root zone is quite different from the soil away from the root zone and the biology of this complex zone has been studied extensively. The term ‘Rhizosphere’ was proposed by Hiltner (1904). The phenomenon of accumulation of microorgani ...
... microbes and their ratio. The chemical and physical nature of the root zone is quite different from the soil away from the root zone and the biology of this complex zone has been studied extensively. The term ‘Rhizosphere’ was proposed by Hiltner (1904). The phenomenon of accumulation of microorgani ...
Lecture 6 - U of L Class Index
... Positive (the most common in eukaryotes, but can be found in bacteria as well): an activator protein is needed to activate the gene expression ...
... Positive (the most common in eukaryotes, but can be found in bacteria as well): an activator protein is needed to activate the gene expression ...
NIH Guidelines for Research Involving rDNA Molecules (NIH
... History of the Guidelines • The NIH Guidelines were implemented in response to public and scientific concern over the emerging science of rDNA technologies in the early 1970’s. • By 1976, NIH had published the first set of guidelines which have been amended over time to allow for greater public acc ...
... History of the Guidelines • The NIH Guidelines were implemented in response to public and scientific concern over the emerging science of rDNA technologies in the early 1970’s. • By 1976, NIH had published the first set of guidelines which have been amended over time to allow for greater public acc ...
document
... excitation light was successfully removed by proper setting of the SPAD activation start timing. Thanks to its single-photon sensitivity and time-resolved measurement capability, faint fluorescence signals were successfully detected from Cy5 labeled DNA molecules. The molecules were placed directly ...
... excitation light was successfully removed by proper setting of the SPAD activation start timing. Thanks to its single-photon sensitivity and time-resolved measurement capability, faint fluorescence signals were successfully detected from Cy5 labeled DNA molecules. The molecules were placed directly ...
Comparative Analysis of Diversity and Similarity Indices with Special
... parameters associated with species abundance and community composition and to assess changes in biological communities due to environmental stress has long been an important aspect of theoretical and applied ecological research [9], [11]. Community structure is a diverse and well developed field. Th ...
... parameters associated with species abundance and community composition and to assess changes in biological communities due to environmental stress has long been an important aspect of theoretical and applied ecological research [9], [11]. Community structure is a diverse and well developed field. Th ...
Disinfectant Regulation, Technologies, Sterility and Validation
... Chemical neutralization – neutralizing the active Dilution - generally not effective alone (alcohols) Filtration – separating the active from the organism Issues Antimicrobial activity of neutralizer (toxicity) Mechanical separation causing damage to cells Validation of neutralization is r ...
... Chemical neutralization – neutralizing the active Dilution - generally not effective alone (alcohols) Filtration – separating the active from the organism Issues Antimicrobial activity of neutralizer (toxicity) Mechanical separation causing damage to cells Validation of neutralization is r ...
Promoter Analysis of the Mouse Sterol Regulatory Element
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
The Origin of Felsic Lavas in the East African Rift Gabriel Akec
... the Turkana (N. Kenya), Ethiopia, and Tanzania, we accept the role of continuous fractional crystallization starting from transitional basaltic parents, and (possibly with role of crustal contamination) for lavas from Turkana and Ethiopia (Peccerillo et al., 2003). This hypothesis is able to explai ...
... the Turkana (N. Kenya), Ethiopia, and Tanzania, we accept the role of continuous fractional crystallization starting from transitional basaltic parents, and (possibly with role of crustal contamination) for lavas from Turkana and Ethiopia (Peccerillo et al., 2003). This hypothesis is able to explai ...
Pursuing DNA Catalysts for Protein Modification
... activities (removal and attachment of phosphoryl groups to side chains). Through all of these efforts, we have learned the importance of careful selection design, including the frequent need to develop specific “capture” reactions that enable the selection process to provide only those DNA sequences t ...
... activities (removal and attachment of phosphoryl groups to side chains). Through all of these efforts, we have learned the importance of careful selection design, including the frequent need to develop specific “capture” reactions that enable the selection process to provide only those DNA sequences t ...
Metabolomics of Genetically Modified Crops
... Despite its important economic potential, authorization and commercialization of GMOs has been always controversial within the scientific community and the public sector. Several aspects of GMOs, including risk assessment, marketing, labeling, and traceability are strictly regulated in the European ...
... Despite its important economic potential, authorization and commercialization of GMOs has been always controversial within the scientific community and the public sector. Several aspects of GMOs, including risk assessment, marketing, labeling, and traceability are strictly regulated in the European ...
Application Note #14 - GE Healthcare Life Sciences
... proteinases. Mass spectrometry has recently proven to be an important methodology for characterizing peptides and proteins, particularly when combined with other techniques, such as gel electrophoresis and amino acid sequence analysis [4]. The coupling of SDS-denaturing gel separation of proteins wi ...
... proteinases. Mass spectrometry has recently proven to be an important methodology for characterizing peptides and proteins, particularly when combined with other techniques, such as gel electrophoresis and amino acid sequence analysis [4]. The coupling of SDS-denaturing gel separation of proteins wi ...
Acoustophoretic sample preparation for PCR in sepsis diagnostics Master’s thesis
... This study was part of an effort to develop a polymerase chain reaction (PCR)-based diagnostic system for detecting and identifying bacteria in blood in sepsis. The blood sample is first prepared by separating the substances that inhibit PCR in blood with acoustophoresis, using ultrasound to direct ...
... This study was part of an effort to develop a polymerase chain reaction (PCR)-based diagnostic system for detecting and identifying bacteria in blood in sepsis. The blood sample is first prepared by separating the substances that inhibit PCR in blood with acoustophoresis, using ultrasound to direct ...