EXCELAPURE 96-Well UF PCR Purification Kit
... Prepare the vacuum manifold1 according to manufacturer's instructions. ...
... Prepare the vacuum manifold1 according to manufacturer's instructions. ...
lect 5- Cloning Vectors
... • Some plasmids are represented by 10 to 100 copies per host ,these are called high copy number plasmids. • Some plasmids maintain 1 to 4 copies per cell and are called low copy number plasmids. • Seldom does the population of plasmids in a bacterium make up more than approx 0.1 to 5.0 percent of th ...
... • Some plasmids are represented by 10 to 100 copies per host ,these are called high copy number plasmids. • Some plasmids maintain 1 to 4 copies per cell and are called low copy number plasmids. • Seldom does the population of plasmids in a bacterium make up more than approx 0.1 to 5.0 percent of th ...
Resistance to the Pseudomonas syringae Effector
... coiled-coil domain (Martin et al., 2003; Meyers et al., 2003; Nimchuk et al., 2003). While the distinction is not absolute, genetically TIR-NBS-LRR (TNL) and coiled-coil-NBS-LRR (CNL) defense pathways can be separated based on the general requirement of TNL proteins for a functional ENHANCED DISEASE ...
... coiled-coil domain (Martin et al., 2003; Meyers et al., 2003; Nimchuk et al., 2003). While the distinction is not absolute, genetically TIR-NBS-LRR (TNL) and coiled-coil-NBS-LRR (CNL) defense pathways can be separated based on the general requirement of TNL proteins for a functional ENHANCED DISEASE ...
CRISPR/Cas9 as a tool for creation of p53 knock
... the CRISPR/Cas9 system to a target gene is outlined in Figure 3: after unique gene sequences on the target gene are identified, a gRNA strand complementary to a target sequence forms an endonuclease by associating with Cas9. This complex seeks out the DNA sequences complementary to the guide strand ...
... the CRISPR/Cas9 system to a target gene is outlined in Figure 3: after unique gene sequences on the target gene are identified, a gRNA strand complementary to a target sequence forms an endonuclease by associating with Cas9. This complex seeks out the DNA sequences complementary to the guide strand ...
Mechanisms of antimicrobial defense in avian eggs
... microorganisms detected in the interior of infected eggs are Gram-negative bacteria (i.e., family Enterobacteriaceae), which are more resistant to the chemical protection of egg proteins like lysozyme (Board 1966). Comprehensively cataloging the microbial communities on and in eggs using both cultur ...
... microorganisms detected in the interior of infected eggs are Gram-negative bacteria (i.e., family Enterobacteriaceae), which are more resistant to the chemical protection of egg proteins like lysozyme (Board 1966). Comprehensively cataloging the microbial communities on and in eggs using both cultur ...
Biology Review
... an imaginary axis, forming a double helix In the DNA double helix, the two backbones run in opposite 5′ → 3′ directions from each other, an arrangement referred to as antiparallel One DNA molecule includes many genes The nitrogenous bases in DNA pair up and form hydrogen bonds: adenine (A) alw ...
... an imaginary axis, forming a double helix In the DNA double helix, the two backbones run in opposite 5′ → 3′ directions from each other, an arrangement referred to as antiparallel One DNA molecule includes many genes The nitrogenous bases in DNA pair up and form hydrogen bonds: adenine (A) alw ...
Members of the IclR family of bacterial transcriptional regulators
... Defining the IclR family A specific profile for the IclR family allows easy identification of IclR family members in different DNA and protein databases The IclR family is named after the member of this group that has been most comprehensively characterized at the genetic and biochemical level – the ...
... Defining the IclR family A specific profile for the IclR family allows easy identification of IclR family members in different DNA and protein databases The IclR family is named after the member of this group that has been most comprehensively characterized at the genetic and biochemical level – the ...
Relationship between codon biased genes, microarray expression
... pneumoniae. Predicted highly expressed (PHE) genes included those encoding glycolytic and fermentative enzymes, sugar-conversion systems and carbohydrate-transporters. Additionally, some genes required for infection that are involved in oxidative metabolism and hydrogen peroxide production were PHE. ...
... pneumoniae. Predicted highly expressed (PHE) genes included those encoding glycolytic and fermentative enzymes, sugar-conversion systems and carbohydrate-transporters. Additionally, some genes required for infection that are involved in oxidative metabolism and hydrogen peroxide production were PHE. ...
Clinical Case Example - Montana State University Extended University
... Tips for Your Project Read this over before the ID virtual lab meeting. During the virtual meetings we will answer questions about testing a hypothesis and on your experimental design. 1. You can work in groups or work by yourself. 2. Develop a hypothesis to be tested. Think of a topic you are inter ...
... Tips for Your Project Read this over before the ID virtual lab meeting. During the virtual meetings we will answer questions about testing a hypothesis and on your experimental design. 1. You can work in groups or work by yourself. 2. Develop a hypothesis to be tested. Think of a topic you are inter ...
Biosensors - Portal UniMAP
... Biosensors using living immobilized microbes play an important part in environmental testing Yeasts such as Trichosporon cutaneum and Artxula adenivorans are the most frequently used species to measure the organic pollution ...
... Biosensors using living immobilized microbes play an important part in environmental testing Yeasts such as Trichosporon cutaneum and Artxula adenivorans are the most frequently used species to measure the organic pollution ...
Intact PTH
... Intact PTH For In Vitro Diagnostic Use The MicroVue PTH EIA measures the amount of intact Parathyroid Hormone (PTH) in experimental samples. PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor co ...
... Intact PTH For In Vitro Diagnostic Use The MicroVue PTH EIA measures the amount of intact Parathyroid Hormone (PTH) in experimental samples. PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor co ...
Multiple Manner Transposons in Flatworms and Hydras Are Related
... the major phyla and classes of invertebrates beyond the Insecta were successfully examined (Table 1). Only the three planarians examined and the two hydra species were positive, and each was positive with all four combinations of PCR primers, that is, they yielded at least one ...
... the major phyla and classes of invertebrates beyond the Insecta were successfully examined (Table 1). Only the three planarians examined and the two hydra species were positive, and each was positive with all four combinations of PCR primers, that is, they yielded at least one ...
BLAST: Basic Local Alignment Search Tool Meng Cao, Arthur Lee
... compare DNA, it is not without its shortcomings. The basic premise behind the algorithm is that it searches for segments of DNA that are likely to be the most similar, rather than comparing each individual section with every other one. This innovation increases the speed with which DNA can be search ...
... compare DNA, it is not without its shortcomings. The basic premise behind the algorithm is that it searches for segments of DNA that are likely to be the most similar, rather than comparing each individual section with every other one. This innovation increases the speed with which DNA can be search ...
Genome-wide analysis of the GRAS gene family in
... development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flow ...
... development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flow ...
The buccale puzzle: The symbiotic nature of endogenous infections
... conditional disease in that fermentable carbohydrates, primarily sucrose, are necessary for the formation of lactic acid (15). Lactic acid is the end product of glycolytic bacterial metabolism. When a pH of less than 5.5 is reached, surface demineralization usually occurs with eventual cavitation. T ...
... conditional disease in that fermentable carbohydrates, primarily sucrose, are necessary for the formation of lactic acid (15). Lactic acid is the end product of glycolytic bacterial metabolism. When a pH of less than 5.5 is reached, surface demineralization usually occurs with eventual cavitation. T ...
2006_1_ivaskik207KBJun 26 2006 10:25:31 AM
... generally lower than those found in undisturbed habitats. Agricultural activities such as plowing, several tillage operations, fertilizing and application of chemical pesticides have dramatical effect on invertebrate animals. Any management practices applied to soils are likely to have some (positiv ...
... generally lower than those found in undisturbed habitats. Agricultural activities such as plowing, several tillage operations, fertilizing and application of chemical pesticides have dramatical effect on invertebrate animals. Any management practices applied to soils are likely to have some (positiv ...
24.5 Nucleic Acids
... DNA typing uses the variation in the DNA of individuals as a basis for creating DNA profiles to identify a person from samples of his or her hair, skin cells, or body fluid. • Because DNA sequences, like fingerprints, are unique for each individual, DNA typing has also been called DNA fingerprinting ...
... DNA typing uses the variation in the DNA of individuals as a basis for creating DNA profiles to identify a person from samples of his or her hair, skin cells, or body fluid. • Because DNA sequences, like fingerprints, are unique for each individual, DNA typing has also been called DNA fingerprinting ...
14_chapter 4
... All the 59 isolated purple bacterial strains (Table 3.2) could not be subjected to the laborious polyphasic characterization, which includes large numbers (about 150) of tests. Hence, for detailed polyphasic characterization, 9 strains (JA194T, JA276T, JA296T, JA248T, JA430T, JA310T, JA531T, JA349T, ...
... All the 59 isolated purple bacterial strains (Table 3.2) could not be subjected to the laborious polyphasic characterization, which includes large numbers (about 150) of tests. Hence, for detailed polyphasic characterization, 9 strains (JA194T, JA276T, JA296T, JA248T, JA430T, JA310T, JA531T, JA349T, ...
Functional genomics analysis of foliar condensed tannin and
... Hybridization was carried out in an automated hybridization station (Tecan, Maennedorf, Switzerland) at 40 °C for 36 h (Sartor et al. 2004) using equal amounts of Cy3- and Cy5-labeled targets. Microarray data analysis The slides were scanned with a GenePix 4000B Scanner (Axon Instruments, Union City ...
... Hybridization was carried out in an automated hybridization station (Tecan, Maennedorf, Switzerland) at 40 °C for 36 h (Sartor et al. 2004) using equal amounts of Cy3- and Cy5-labeled targets. Microarray data analysis The slides were scanned with a GenePix 4000B Scanner (Axon Instruments, Union City ...
Leishmania donovani - Oxford Academic
... strand cleavage and religation reactions. These two lobes are covalently linked through a continuous helical chain on one side of the DNA molecule labeled the ‘connector’, and touch each other in a small contact region, similar to the ‘lip’ structure in the human topoisomerase I (32). The coiled-coi ...
... strand cleavage and religation reactions. These two lobes are covalently linked through a continuous helical chain on one side of the DNA molecule labeled the ‘connector’, and touch each other in a small contact region, similar to the ‘lip’ structure in the human topoisomerase I (32). The coiled-coi ...
Introductory Mycology BI 432/532 Lecture 2: Overview of
... Plantarum. (B) Detail from classic illustration of spore germination in Tilletia caries by Prévost (1807). (C) Carl Ludwig Willdenow (1765–1812), the scientist who coined the term ‘hypha’ in 1810. (D) Illustration of spore germination and hyphal development by Oscar Brefeld (1872). (E) Max Otto Rein ...
... Plantarum. (B) Detail from classic illustration of spore germination in Tilletia caries by Prévost (1807). (C) Carl Ludwig Willdenow (1765–1812), the scientist who coined the term ‘hypha’ in 1810. (D) Illustration of spore germination and hyphal development by Oscar Brefeld (1872). (E) Max Otto Rein ...
Identifying producers of antibacterial compounds by
... These modifications can affect pharmacology and target affinity and, in the case of antibiotics, mitigate resistance. One of the main challenges in the field is the identification of microbes with the biosynthetic capacity to produce desired compound classes. It has been estimated, for example, that ...
... These modifications can affect pharmacology and target affinity and, in the case of antibiotics, mitigate resistance. One of the main challenges in the field is the identification of microbes with the biosynthetic capacity to produce desired compound classes. It has been estimated, for example, that ...
Journal of Molecular Microbiology and Biotechnology
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
RNA polymerase
... • Does NOT require a primer; can start RNA synthesis at a site on the DNA template ...
... • Does NOT require a primer; can start RNA synthesis at a site on the DNA template ...