Dynamic DNA nanotechnology using strand displacement reactions
... kinetics of a toehold composed of roughly equal numbers of all 4 nucleotides. The gray region spanned by the green and red traces roughly show the range of potential kinetics based on toehold length. The progress of strand displacement reactions is typically assayed using fluorescence, via either re ...
... kinetics of a toehold composed of roughly equal numbers of all 4 nucleotides. The gray region spanned by the green and red traces roughly show the range of potential kinetics based on toehold length. The progress of strand displacement reactions is typically assayed using fluorescence, via either re ...
Carcinoembryonic Antigens - The Journal of Cell Biology
... al., 1987). Use of the CEA partial cDNA insert, LV7, as probe identified multiple hybridizing RNA bands with approximate molecular sizes of 3.9, 3.7, 2.2, and 1.8 kb (Fig. 1, lane a). This pattern also recurs in the colon adenocarcinoma cell line HT-29 (Fig. 1, lane b), which in addition expresses m ...
... al., 1987). Use of the CEA partial cDNA insert, LV7, as probe identified multiple hybridizing RNA bands with approximate molecular sizes of 3.9, 3.7, 2.2, and 1.8 kb (Fig. 1, lane a). This pattern also recurs in the colon adenocarcinoma cell line HT-29 (Fig. 1, lane b), which in addition expresses m ...
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... with markings to assist residue counting. The low-complexity and high-complexity segments are in lower- and upper-case characters respectively. ...
... with markings to assist residue counting. The low-complexity and high-complexity segments are in lower- and upper-case characters respectively. ...
Gene flow reverses an adaptive cline in a coevolving host
... 2002; Mizoguchi et al. 2003; Morgan et al. 2005). Unlike most field systems, laboratory experiments with microbes allow for direct control over dispersal and the environment in which the organisms interact. In many field systems, environmental gradients are multivariate—for example, latitudinal grad ...
... 2002; Mizoguchi et al. 2003; Morgan et al. 2005). Unlike most field systems, laboratory experiments with microbes allow for direct control over dispersal and the environment in which the organisms interact. In many field systems, environmental gradients are multivariate—for example, latitudinal grad ...
475 S07 background questions
... 123. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 124. Describe two techniques to introduce recombinant DNA into eukaryotic cells. 125. Describe the polymerase chain reaction (PCR) and explain the advantages and limitations of ...
... 123. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 124. Describe two techniques to introduce recombinant DNA into eukaryotic cells. 125. Describe the polymerase chain reaction (PCR) and explain the advantages and limitations of ...
Bacterial Transformation with Green Fluorescent Protein
... You will be transforming a bacterium, E. coli, which lives in the human gut. Genetically, E. coli is relatively simple and well understood. Its genetic material consists mostly of one large circle of DNA between 3‐5 million base pairs in length. Also present are small loops of DNA called plasmids, ...
... You will be transforming a bacterium, E. coli, which lives in the human gut. Genetically, E. coli is relatively simple and well understood. Its genetic material consists mostly of one large circle of DNA between 3‐5 million base pairs in length. Also present are small loops of DNA called plasmids, ...
the Gene Ontology
... GO annotation relies on computational methods (rapid) functional literature exists for many genes/proteins prior to genome sequencing GO annotation does not rely on a completed genome sequence! ...
... GO annotation relies on computational methods (rapid) functional literature exists for many genes/proteins prior to genome sequencing GO annotation does not rely on a completed genome sequence! ...
8-Cell and Molecular Biology (Transcription)
... for a wide variety of processes in the cell. For example: One of those RNAs, the template carried by the enzyme telomerase Table 6.1 Alberts 5th Ed • Each transcribed segment of DNA is called a transcription unit ...
... for a wide variety of processes in the cell. For example: One of those RNAs, the template carried by the enzyme telomerase Table 6.1 Alberts 5th Ed • Each transcribed segment of DNA is called a transcription unit ...
Strategies for Attaching Oligonucleotides to Solid Supports
... The surface density of the oligonucleotide probe is expected to be an important parameter of any array. A low surface coverage will yield a correspondingly low hybridization signal and decrease the hybridization rate. Conversely, high surface densities may result in steric interference between the c ...
... The surface density of the oligonucleotide probe is expected to be an important parameter of any array. A low surface coverage will yield a correspondingly low hybridization signal and decrease the hybridization rate. Conversely, high surface densities may result in steric interference between the c ...
- Bridgewater College WordPress
... belonged to the phylum Micrococcus and the genus Luteus. Since the 16S rDNA results were unsuccessful for this isolate, tests were performed based upon a dichotomous key (10). Since the bacterium was in fact Gram positive, occurred in tetrad-occurring groups, was catalase positive, and had a yellow ...
... belonged to the phylum Micrococcus and the genus Luteus. Since the 16S rDNA results were unsuccessful for this isolate, tests were performed based upon a dichotomous key (10). Since the bacterium was in fact Gram positive, occurred in tetrad-occurring groups, was catalase positive, and had a yellow ...
Diagnostic protocol for
... cultivars (Goto, 1992). Attacks of Phyllocnistis citrella, the citrus leaf miner increases the susceptibility of leaves to citrus canker. Xcc can survive in diseased plant tissues, as an epiphyte on host and non-host plants, and as a saprophyte on straw mulch or in soil. However, overwintering lesio ...
... cultivars (Goto, 1992). Attacks of Phyllocnistis citrella, the citrus leaf miner increases the susceptibility of leaves to citrus canker. Xcc can survive in diseased plant tissues, as an epiphyte on host and non-host plants, and as a saprophyte on straw mulch or in soil. However, overwintering lesio ...
Vectors for Even Larger Genomic DNA Inserts
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
Diversity, Sources, and Detection of Human Bacterial Pathogens in
... outbreaks of known etiology in marine animals, and (iii) testing of marine environments for the presence of pathogen populations. In particular, surveys of environmental microbial communities based on 16S ribosomal RNA (rRNA) gene sequence diversity have revealed a large number of organisms closely ...
... outbreaks of known etiology in marine animals, and (iii) testing of marine environments for the presence of pathogen populations. In particular, surveys of environmental microbial communities based on 16S ribosomal RNA (rRNA) gene sequence diversity have revealed a large number of organisms closely ...
Chronic nitrogen deposition alters the structure and function of
... plot and divided the sum by the total collected area (individuals/m2). The second method employed the sum of the abundance within each plot divided by the total dried mass of the six subsamples (individuals/g dried litter). We also calculated the density-by-area abundance for the microarthropod grou ...
... plot and divided the sum by the total collected area (individuals/m2). The second method employed the sum of the abundance within each plot divided by the total dried mass of the six subsamples (individuals/g dried litter). We also calculated the density-by-area abundance for the microarthropod grou ...
Module Document
... while trying to supply themselves with sufficient food and available oxygen, overcoming competition and predation, and producing gametes for reproduction. They are unable to control the physical environment, and none of the biological challenges is easily met. Any additional physical or biological s ...
... while trying to supply themselves with sufficient food and available oxygen, overcoming competition and predation, and producing gametes for reproduction. They are unable to control the physical environment, and none of the biological challenges is easily met. Any additional physical or biological s ...
Mass spectrometry - 123seminarsonly.com
... to overlapping bands, or to indistinguishable smears representing multiple unresolved components. Bands in different lanes that end up at the same distance from the top contain molecules that passed through the gel with the same speed, which usually means they are approximately the same size. There ...
... to overlapping bands, or to indistinguishable smears representing multiple unresolved components. Bands in different lanes that end up at the same distance from the top contain molecules that passed through the gel with the same speed, which usually means they are approximately the same size. There ...
PowerPoint 演示文稿 - Xiamen University
... (c) This merodiploid has one wild-type operon (top) and one operon (bottom) with a mutant repressor gene (lS) those product (yellow) cannot bind inducer. The mutant repressor therefore binds irreversibly to both operators and renders both operons uninducible. This mutation is therefore dominant. Not ...
... (c) This merodiploid has one wild-type operon (top) and one operon (bottom) with a mutant repressor gene (lS) those product (yellow) cannot bind inducer. The mutant repressor therefore binds irreversibly to both operators and renders both operons uninducible. This mutation is therefore dominant. Not ...
Chemistry written examination 1 2008–2011
... ii. label the DNA fragments according to their size. 1 + 1 = 2 marks Total 5 marks ...
... ii. label the DNA fragments according to their size. 1 + 1 = 2 marks Total 5 marks ...
Microplastic pollution in the surface waters of the Laurentian Great
... et al., 2010). Polyethylene and polypropylene microbeads, used in many consumer facial cleansers, have been identified as potential contributors to marine pollution (Gregory, 1996; Fendall and Sewell, 2009). Textile laundering facilities are also potential sources of microplastic fibers (Browne et al. ...
... et al., 2010). Polyethylene and polypropylene microbeads, used in many consumer facial cleansers, have been identified as potential contributors to marine pollution (Gregory, 1996; Fendall and Sewell, 2009). Textile laundering facilities are also potential sources of microplastic fibers (Browne et al. ...