Chromium Incorporated in RNA and DNA
... the cells investigated (data not shown). The number of Cr atoms bound to DNA molecules were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µ ...
... the cells investigated (data not shown). The number of Cr atoms bound to DNA molecules were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µ ...
Avian infectious laryngotracheitis
... In the subacute form, the onset of illness is slower and respiratory signs may extend over some days before deaths are seen. The morbidity is high but the mortality is lower than in the peracute form, between 10% and 30%. Post-mortem findings are less severe and consist of mucoid exudate with or wi ...
... In the subacute form, the onset of illness is slower and respiratory signs may extend over some days before deaths are seen. The morbidity is high but the mortality is lower than in the peracute form, between 10% and 30%. Post-mortem findings are less severe and consist of mucoid exudate with or wi ...
Biotechnology Explorer - Bio-Rad
... explore mechanisms of gene regulation (Appendix D) and genetic selection. And, the entire process is observable with an inexpensive long-wave UV lamp or with the provided pen-light. In order for your students to gain the most from this experiment, they should know what a gene is and understand the r ...
... explore mechanisms of gene regulation (Appendix D) and genetic selection. And, the entire process is observable with an inexpensive long-wave UV lamp or with the provided pen-light. In order for your students to gain the most from this experiment, they should know what a gene is and understand the r ...
Ribosome engineering to promote new crystal forms
... conserved: the hidden surface area varies between 140 and 460 Å2 and the relative position of the C-terminal domain of L9 differs by up to 20 Å. In the three most extensive packing interactions, with more than 400 Å2 hidden surface area (Table 1), the position of L9-C differs by about 4 Å (Fig. ...
... conserved: the hidden surface area varies between 140 and 460 Å2 and the relative position of the C-terminal domain of L9 differs by up to 20 Å. In the three most extensive packing interactions, with more than 400 Å2 hidden surface area (Table 1), the position of L9-C differs by about 4 Å (Fig. ...
Cyclohexanone - Health and Safety Laboratory
... into diethyl ether and analysed by gas chromatography with flame ionisation detection or selected ion monitoring mass spectrometry. ...
... into diethyl ether and analysed by gas chromatography with flame ionisation detection or selected ion monitoring mass spectrometry. ...
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND
... Deoxyribonucleic acid, or DNA, is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information and DNA is often compared to a set of blueprints, since it contains th ...
... Deoxyribonucleic acid, or DNA, is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information and DNA is often compared to a set of blueprints, since it contains th ...
A Primer on Gene Regulation
... A simple experimental procedure for measuring the binding constant is the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end ...
... A simple experimental procedure for measuring the binding constant is the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end ...
In-situ Protocol for larval/pupal/adult tissues
... DIG-POD-HRP (used 1:500-1000). Both work but sheep has a higher background signal in our hands. The DIG antibodies are fairly specific and give good signal after 2 hours at room temperature. However, they can be incubated at 4°C overnight without negative effects. Primary antibodies for other detect ...
... DIG-POD-HRP (used 1:500-1000). Both work but sheep has a higher background signal in our hands. The DIG antibodies are fairly specific and give good signal after 2 hours at room temperature. However, they can be incubated at 4°C overnight without negative effects. Primary antibodies for other detect ...
Assessing biodiversity in arable farmland by means of indicators: an
... were valuable tools to assess functional diversity (genetic structure, abundance vs level of expression of functional microbial genes). The development of multiparametric indices integrating these data is now needed to better understand the relationships between soil microbial diversity and function ...
... were valuable tools to assess functional diversity (genetic structure, abundance vs level of expression of functional microbial genes). The development of multiparametric indices integrating these data is now needed to better understand the relationships between soil microbial diversity and function ...
PBI 6 Features on Teacher`s Map 2-08.qxp
... sequence is immediately upstream from the cleavage and polyadenylation site. Nucleotides 63742 to 63743: Cleavage and polyadenylation site Just 5’ to the cleavage/polyadenylation site, the dinucleotide CA is the preferred sequence. Though this sequence is not required, in β-globin, the consensus seq ...
... sequence is immediately upstream from the cleavage and polyadenylation site. Nucleotides 63742 to 63743: Cleavage and polyadenylation site Just 5’ to the cleavage/polyadenylation site, the dinucleotide CA is the preferred sequence. Though this sequence is not required, in β-globin, the consensus seq ...
A Research Strategy to Understand the Mechanisms that Govern
... 4. Regulation of target gene expression After identifying the target gene, the next step is to clarify the mechanism responsible for the change in the position-specific expression level. Inserting transposons into the promoter region of the target gene causes a decline in transcription activity whil ...
... 4. Regulation of target gene expression After identifying the target gene, the next step is to clarify the mechanism responsible for the change in the position-specific expression level. Inserting transposons into the promoter region of the target gene causes a decline in transcription activity whil ...
Chemical Synthesis of Oligonucleotides
... Scale refers to the amount of starting material which is composed solely of the 3’-most nucleotide of a sequence attached to a solid support used to make the oligonucleotide. Yield refers to the amount of final product recovered after all the synthesis, processing, and purification steps associated ...
... Scale refers to the amount of starting material which is composed solely of the 3’-most nucleotide of a sequence attached to a solid support used to make the oligonucleotide. Yield refers to the amount of final product recovered after all the synthesis, processing, and purification steps associated ...
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education
... Structure of the Holoenzyme-DNA Complex Crystal structure of T. aquaticus holoenzyme-DNA complex as an open promoter complex reveals: – DNA is bound mainly to s-subunit – Interactions between amino acids in region 2.4 of s and -10 box of promoter are possible – 3 highly conserved aromatic amino aci ...
... Structure of the Holoenzyme-DNA Complex Crystal structure of T. aquaticus holoenzyme-DNA complex as an open promoter complex reveals: – DNA is bound mainly to s-subunit – Interactions between amino acids in region 2.4 of s and -10 box of promoter are possible – 3 highly conserved aromatic amino aci ...
the free amino acids in the sediments of toronto harbor
... Dissolved free amino’ acids were extracted from a minimum of four core samples of sediment per month, obtained frojm Hanlan’s Point or Keating Channel, Toronto Harbor, with the K.B. pneumatic corer ( Brinkhurst et al. 1969). The upper 6 cm of each core was extruded and placed in a Mason jar to which ...
... Dissolved free amino’ acids were extracted from a minimum of four core samples of sediment per month, obtained frojm Hanlan’s Point or Keating Channel, Toronto Harbor, with the K.B. pneumatic corer ( Brinkhurst et al. 1969). The upper 6 cm of each core was extruded and placed in a Mason jar to which ...
Pol /Primase, Pol ε Pol ε α MIT Department of Biology 7.28, Spring
... replication, propose a function for the proteins in your purified fraction. Be sure to explain how these functions explain the results of your studies of 3H-thymidine incorporation by the mutant strain. To reiterate first part of question (I thought it was a little confusing): So you go back to your ...
... replication, propose a function for the proteins in your purified fraction. Be sure to explain how these functions explain the results of your studies of 3H-thymidine incorporation by the mutant strain. To reiterate first part of question (I thought it was a little confusing): So you go back to your ...
- UEA Digital Repository
... 2.13 Cloning of PCR products ..................................................................................59 2.14 RFLP analysis of cloned sequences from 13C-MMA-SIP incubations .............60 2.15 DNA sequencing and phylogenetic analysis .....................................................60 ...
... 2.13 Cloning of PCR products ..................................................................................59 2.14 RFLP analysis of cloned sequences from 13C-MMA-SIP incubations .............60 2.15 DNA sequencing and phylogenetic analysis .....................................................60 ...
Transfer of DNA from Genetically Modified Organisms (GMOs)
... manipulated genetic material from GMOs to humans. This ability has been evaluated in a series of experiments. However, no toxic or documented genetic effects have been reported. At present, it is tested if recombinant DNA constructions can be administrated to humans in order to obtain vaccine respon ...
... manipulated genetic material from GMOs to humans. This ability has been evaluated in a series of experiments. However, no toxic or documented genetic effects have been reported. At present, it is tested if recombinant DNA constructions can be administrated to humans in order to obtain vaccine respon ...
The Science of Transgenics
... Ex. – modified EPSP synthase gene (encodes a protein that functions even when plant is treated with Roundup) Transgenic – an organism containing a transgene introduced by technological (not breeding) methods ...
... Ex. – modified EPSP synthase gene (encodes a protein that functions even when plant is treated with Roundup) Transgenic – an organism containing a transgene introduced by technological (not breeding) methods ...
A Simple Introduction to NCBI BLAST
... of the model fruit fly Drosophila melanogaster) contains region(s) with sequence similarity to any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of ...
... of the model fruit fly Drosophila melanogaster) contains region(s) with sequence similarity to any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of ...
Supplemental Methods
... 75% ACN in PBS/CHAPS per well. The total elapsed time during the wash steps was approximately 10 min (i.e., shorter than the half off-time of the anti-peptide antibodies). Finally, the bead-antibody complex reached the elution plate where the target peptides were eluted in 13 L of 0.1% formic acid. ...
... 75% ACN in PBS/CHAPS per well. The total elapsed time during the wash steps was approximately 10 min (i.e., shorter than the half off-time of the anti-peptide antibodies). Finally, the bead-antibody complex reached the elution plate where the target peptides were eluted in 13 L of 0.1% formic acid. ...
The Protozoa
... of life’s various functions must be performed within the individual protozoan, some morphological and physiological features are unique to protozoan cells. In some species the cytoplasm immediately under the plasma membrane is semisolid or gelatinous, giving some rigidity to the cell body. It is ter ...
... of life’s various functions must be performed within the individual protozoan, some morphological and physiological features are unique to protozoan cells. In some species the cytoplasm immediately under the plasma membrane is semisolid or gelatinous, giving some rigidity to the cell body. It is ter ...