post-transcription
... makes many mRNAs. Can we determine which part of the genome encodes for each mRNA by making a DNA:RNA hybrid? Experiment: Isolate Adenovirus genomic DNA, isolate one adenovirus mRNA, hybridize and then look by EM at where the RNA hybridizes (binds) to the genomic DNA. Surprise: The RNA is generated ...
... makes many mRNAs. Can we determine which part of the genome encodes for each mRNA by making a DNA:RNA hybrid? Experiment: Isolate Adenovirus genomic DNA, isolate one adenovirus mRNA, hybridize and then look by EM at where the RNA hybridizes (binds) to the genomic DNA. Surprise: The RNA is generated ...
PDF - FEMS Microbiology Letters
... showed 85% identity to Ent. faecalis tyrosine decarboxylase. The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 5 ...
... showed 85% identity to Ent. faecalis tyrosine decarboxylase. The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 5 ...
Brooker Genetics 5e Sample Chapter 16
... it stimulates its own expression. Furthermore, if the gene that encodes this transcription factor is present in two copies in the cell, both copies will be activated because the transcription factor is a diffusible protein and many of these proteins are made when the gene is expressed. This pattern ...
... it stimulates its own expression. Furthermore, if the gene that encodes this transcription factor is present in two copies in the cell, both copies will be activated because the transcription factor is a diffusible protein and many of these proteins are made when the gene is expressed. This pattern ...
A structural determinant in the uracil DNA glycosylase superfamily
... pairs and single-stranded uracil-containing DNA (2,5). Escherichia coli MUG, which belongs to family 2, is named for its UDG activity on mismatched T/U, G/U and C/U base pairs (8–11). In addition to its UDG activity on mismatched base pairs, E. coli MUG is also a robust xanthine DNA glycosylase (12) ...
... pairs and single-stranded uracil-containing DNA (2,5). Escherichia coli MUG, which belongs to family 2, is named for its UDG activity on mismatched T/U, G/U and C/U base pairs (8–11). In addition to its UDG activity on mismatched base pairs, E. coli MUG is also a robust xanthine DNA glycosylase (12) ...
Molecular Characterization of NADH-Dependent
... RNase H activity. This screening produced numerous hybridizing recombinant phage that contained inserts longer than those previously obtained, including one which was of a size that made it a candidate for a full-length cDNA clone (-7.2 kb). The complete nucleotide sequence of this insert (7212 bp) ...
... RNase H activity. This screening produced numerous hybridizing recombinant phage that contained inserts longer than those previously obtained, including one which was of a size that made it a candidate for a full-length cDNA clone (-7.2 kb). The complete nucleotide sequence of this insert (7212 bp) ...
emboj200897-sup
... substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for all wild type Etk and mutant samples used in this study. The fully dephosphorylated protein sa ...
... substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for all wild type Etk and mutant samples used in this study. The fully dephosphorylated protein sa ...
supplementary methods
... BGT147 has a hybrid intron 2 that contains the 5' A-globin region, Oct-1 site and 3' globin region created in several steps. First, the 5' A intron 2 was PCR amplified using B54 (S) and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI sit ...
... BGT147 has a hybrid intron 2 that contains the 5' A-globin region, Oct-1 site and 3' globin region created in several steps. First, the 5' A intron 2 was PCR amplified using B54 (S) and B54 Dra (AS) primers (Table S1) using BGT54 DNA as template. B54 Dra (AS) primer was used to create a DraI sit ...
Document
... 9.4 How the DNA Molecule Replicates The two DNA strands are held together by weak hydrogen bonds between complementary base pairs A and T C and G ...
... 9.4 How the DNA Molecule Replicates The two DNA strands are held together by weak hydrogen bonds between complementary base pairs A and T C and G ...
Sequencing breakthroughs for genomic ecology and
... and to identify unculturable microorganisms from their DNA sequence (reviewed by Handelsman 2004) have all allowed great advances in their respective fields and in many cases had much broader impacts on society. However, all these techniques are based on the analysis of a tiny fragment of an organis ...
... and to identify unculturable microorganisms from their DNA sequence (reviewed by Handelsman 2004) have all allowed great advances in their respective fields and in many cases had much broader impacts on society. However, all these techniques are based on the analysis of a tiny fragment of an organis ...
The Living World - Chapter 9 - McGraw Hill Higher Education
... 9.4 How the DNA Molecule Replicates The two DNA strands are held together by weak hydrogen bonds between complementary base pairs A and T C and G ...
... 9.4 How the DNA Molecule Replicates The two DNA strands are held together by weak hydrogen bonds between complementary base pairs A and T C and G ...
Answers - Study of Life
... Replacement of a lysine with a glycine in a protein could result in all of the following EXCEPT: A. a change in the quaternary structure of the protein B. a change in the secondary structure of the protein C. a loss of catalytic activity of the protein D. a loss of a negatively charged side chain E. ...
... Replacement of a lysine with a glycine in a protein could result in all of the following EXCEPT: A. a change in the quaternary structure of the protein B. a change in the secondary structure of the protein C. a loss of catalytic activity of the protein D. a loss of a negatively charged side chain E. ...
A0708 - ICES
... 1025 phytoplankters (average diameter: 2 µm) that exist at any one instant in the euphotic zone (average depth: 100 m). Using the Earth and the Moon as reference points, he calculated that the string of phytoplankton cells would extend from the Earth to the Moon and back (i.e. 770 000 km roundtrips) ...
... 1025 phytoplankters (average diameter: 2 µm) that exist at any one instant in the euphotic zone (average depth: 100 m). Using the Earth and the Moon as reference points, he calculated that the string of phytoplankton cells would extend from the Earth to the Moon and back (i.e. 770 000 km roundtrips) ...
INDECO: Results of the investigation of modelling methods and
... functional one. Such a relationship cannot be tested using solely descriptive data. Consider the example of a freshwater outfall that discharges into an estuary. Away from the outfall, the concentration of many different contaminants decreases and species richness increases. Hypothetically, the loss ...
... functional one. Such a relationship cannot be tested using solely descriptive data. Consider the example of a freshwater outfall that discharges into an estuary. Away from the outfall, the concentration of many different contaminants decreases and species richness increases. Hypothetically, the loss ...
Methods for identifying microRNA binding motifs
... Functional importance of miRNA binding in post-transcriptional gene regulation drives effort in identifying conserved binding motifs But non-canonical motifs dominate over those based on seed sequence Experimental approaches based on RISC complex formation and immunoprecipitation help isolate miRNA- ...
... Functional importance of miRNA binding in post-transcriptional gene regulation drives effort in identifying conserved binding motifs But non-canonical motifs dominate over those based on seed sequence Experimental approaches based on RISC complex formation and immunoprecipitation help isolate miRNA- ...
A Pneumocystis carinii multi-gene family with
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...
Comparison of good-and bad-quality cork: application of high
... information obtained from ESTs is a practical means of gene discovery. Transcriptome obtained by high-throughput or EST sequencing offers an efficient means of generating functional genomic data for non-model organisms (Parchman et al., 2010) because it provides functional information that often cor ...
... information obtained from ESTs is a practical means of gene discovery. Transcriptome obtained by high-throughput or EST sequencing offers an efficient means of generating functional genomic data for non-model organisms (Parchman et al., 2010) because it provides functional information that often cor ...
The effect of extrinsic mortality on genome size evolution in
... Mortality has a significant role in prokaryotic ecology and evolution, yet the impact of variations in extrinsic mortality on prokaryotic genome evolution has received little attention. We used both mathematical and agent-based models to reveal how variations in extrinsic mortality affect prokaryoti ...
... Mortality has a significant role in prokaryotic ecology and evolution, yet the impact of variations in extrinsic mortality on prokaryotic genome evolution has received little attention. We used both mathematical and agent-based models to reveal how variations in extrinsic mortality affect prokaryoti ...
The costs of environmental degradation in the Marine Strategy
... context of the Marine Strategy Framework Directive (MSFD). The MSFD represents the environmental component of the European integrated marine approach (2008/56/EC) and establishes a legislative framework for community action in the area of marine environmental policy. The ultimate aim is to design a ...
... context of the Marine Strategy Framework Directive (MSFD). The MSFD represents the environmental component of the European integrated marine approach (2008/56/EC) and establishes a legislative framework for community action in the area of marine environmental policy. The ultimate aim is to design a ...
The Microbiology of Bad Breath and Periodontitis
... oral malodor to periodontitis: Evidence of independence in discrete subpopulations. Journal of Periodontology 1994; 65:37-46. Rosenberg Mel. The science of Bad Breath. Scientific American 2002; 72-79. Bruce P,Boches S, Galvin J. Bacterial Diversity in human subgingival plaque. Journal of Bacteriolog ...
... oral malodor to periodontitis: Evidence of independence in discrete subpopulations. Journal of Periodontology 1994; 65:37-46. Rosenberg Mel. The science of Bad Breath. Scientific American 2002; 72-79. Bruce P,Boches S, Galvin J. Bacterial Diversity in human subgingival plaque. Journal of Bacteriolog ...
DEPARTMENT OF BIOLOGY UNDERGRADUATE COURSE Animal
... macroevolution and molecular systematics will be consulted and discussed. (Instructor: T. Y. Chiang) Macroevolution: 2 credits This class, for Master and Ph.D. graduate students, is focused on the evolution of organisms at the species and higher taxonomic levels. Several topics are discussed: Darwin ...
... macroevolution and molecular systematics will be consulted and discussed. (Instructor: T. Y. Chiang) Macroevolution: 2 credits This class, for Master and Ph.D. graduate students, is focused on the evolution of organisms at the species and higher taxonomic levels. Several topics are discussed: Darwin ...
Soil microbes and their contribution to soil services
... Saprotrophic fungi produce a wide range of enzymes, including amylases, proteases, lipases, and phosphatases. These enzymes are produced by hyphae at the front of the mycelium as it grows through its substrate. From a single germinated spore, the mycelium will often grow radially outwards creating a ...
... Saprotrophic fungi produce a wide range of enzymes, including amylases, proteases, lipases, and phosphatases. These enzymes are produced by hyphae at the front of the mycelium as it grows through its substrate. From a single germinated spore, the mycelium will often grow radially outwards creating a ...