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Manual - The University of Iowa
Manual - The University of Iowa

... the partially (or fully) deleted E3 region. With a portion of the E3 deleted, an additional gene cassette can be added. The VVC offers several very convenient viral backbone plasmids with and without reporters for investigators to choose from. No cloning of these plasmids is necessary unless you hav ...
limiting resources and the regulation of diversity in phytoplankton
limiting resources and the regulation of diversity in phytoplankton

A Rapid UPLC™ MS/MS Method for Determining Specific
A Rapid UPLC™ MS/MS Method for Determining Specific

... buffer was added. The tubes were mixed thoroughly at which time the samples were ready for analysis1. ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv

... enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransferase (AT3) gene is proposed as a gene encode CS enzyme [10]. This statement is supported by Le ...
Next-Generation Sequencing: an overview of technologies and
Next-Generation Sequencing: an overview of technologies and

... Next-gen sequencing technologies • With massively parallel sequencing new methods for sequencing template preparation is required • Current NGS platforms utilize clonal amplification on solid supports via two main methods: – emulsion PCR (emPCR) – bridge amplification (DNA cluster generation) ...
Under Our Feet: Soil Microorganisms as Primary Drivers of Essential
Under Our Feet: Soil Microorganisms as Primary Drivers of Essential

... themselves into fibrous strings called hyphae. The hyphae then form groups called mycelium which are less than 0.8mm wide but can get as long as several metres. They are helpful, but could also be harmful, to soil organisms. Fungi are helpful because they have the ability to break down nutrients tha ...
Molecular evolution of paclitaxel biosynthetic genes TS and
Molecular evolution of paclitaxel biosynthetic genes TS and

What Are You Drinking, TEA?
What Are You Drinking, TEA?

... I expect to find several different micro organisms that are in tea. The reason for this is because tea is exposed to heat for long periods of time.  I expect to find several different fascinating organisms  The benefits of this experiment will better the knowledge of tea and its content. ...
Eric Davis Project Advisor:  Breck Bowden
Eric Davis Project Advisor: Breck Bowden

... critical breeding habitat for globally pressured amphibian species and support diverse invertebrate communities. These systems may also be particularly efficient in methylating Hg, as they are characterized by low pH conditions and high amounts of dissolved organic carbon and these parameters have b ...
Raven/Johnson Biology 8e Chapter 14 - Answers 1.
Raven/Johnson Biology 8e Chapter 14 - Answers 1.

... b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of proteins will not alter DNA. c. The nonpathogenic cells remain nonpathogenic. If the DNA is digested, it will not be transferred and no transformation will occur. 2. Imagine that you have identified the sequence 5′-TTATAAAGC ...
Raven/Johnson Biology 8e
Raven/Johnson Biology 8e

... b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of proteins will not alter DNA. c. The nonpathogenic cells remain nonpathogenic. If the DNA is digested, it will not be transferred and no transformation will occur. 2. Imagine that you have identified the sequence 5′-TTATAAAGC ...
Alu Background Information
Alu Background Information

... Most Alu mutations are "fixed," meaning that both of the paired chromosomes have an insertion at the same locus (position). However, a number of human-specific Alus are dimorphic – an insertion may be present or absent on each of the paired chromosomes of different people. These dimorphic Alus inser ...
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration

Ribosomes: Cashing in on crystals
Ribosomes: Cashing in on crystals

... revealed its overall shape (at approximately 15–20 Å resolution), the 5 Å map shows its knarled surface, complete with numerous nooks and crannies (Figure 1). The electron-dense phosphate backbone of rRNA is distinctive at 5 Å resolution, enabling Ban et al. [1] to model more than 300 base pairs of ...
Non-homologous Recombination of Deoxyribonucleoside Kinases
Non-homologous Recombination of Deoxyribonucleoside Kinases

Brief Rapid Communications
Brief Rapid Communications

... therapy. First, this vector can only accept transgenes less than '4.5 kb in length. Second, current techniques do not allow the convenient production of large amounts of rAAV. Finally, although our studies have demonstrated efficient transduction of cardiomyocytes after 15 minutes of coronary artery ...
Editorial Novel Computing Technologies for Bioinformatics and
Editorial Novel Computing Technologies for Bioinformatics and

... In the past, many computing technologies have been proposed and utilized to accelerate biologists/chemists to analyze biological and chemical data, such as homology detection, evolutionary analysis, function prediction, computer-aided drug design, and cheminformatics. Leveraging a power of these tec ...
VITREOUS BIOPSY FOR THE DIAGNOSIS OF UVEITIS
VITREOUS BIOPSY FOR THE DIAGNOSIS OF UVEITIS

Identification of a Fluorescent Protein from Rhacostoma Atlantica
Identification of a Fluorescent Protein from Rhacostoma Atlantica

A unique segment of the hepatitis B virus group A genotype
A unique segment of the hepatitis B virus group A genotype

Appendix – Biology for Bioinformatics Fig A1.1 A typical
Appendix – Biology for Bioinformatics Fig A1.1 A typical

... Fig. A1.8 Formation of peptide bond and rotational freedom in it ...
Transfer of genetic material between the chloroplast and nucleus
Transfer of genetic material between the chloroplast and nucleus

... nuclear genome data reported that the plant nuclear genome is in equilibrium between frequent integration and rapid elimination of the chloroplast genome and that the pericentromeric regions play a significant role in facilitating the chloroplast–nuclear DNA flux. This equilibrium between integratio ...
“The only UNG useful in RT-PCR”
“The only UNG useful in RT-PCR”

National University of Life and Environmental Sciences of Ukraine
National University of Life and Environmental Sciences of Ukraine

... The science of microbiology is the study of microorganisms and their activities. It is concerned with their form, structure, reproduction, physiology, metabolism and identification. It includes the study of their distribution in nature, their relationship to each other and to other living things, th ...
cloning of gs1 gene encodes glutamine synthetase 1 and
cloning of gs1 gene encodes glutamine synthetase 1 and

... - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 transgenic plants have been reported in many researches. In this research, in order to prepare for generating GS1 ...
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