Minireview Alpha Satellite and the Quest for the Human Centromere
... within extensive phylogenies that contain both closely related and distant species that are accessible to experimental manipulation. Lessons learned from centromere studies may be relevant to understanding other chromosomal functions, such as homolog pairing, long-distance regulation of gene express ...
... within extensive phylogenies that contain both closely related and distant species that are accessible to experimental manipulation. Lessons learned from centromere studies may be relevant to understanding other chromosomal functions, such as homolog pairing, long-distance regulation of gene express ...
Analysis of the first polar body: preconception genetic
... (Figures 2 and 3); one was PI type M (Figure 3, slot 2B), two were PI type Z (Figure 2, upper polar body, and Figure 3, slot 2C) and two were heterozygous due to crossing over (Figure 2, lower polar body; Figure 3, slot IB). The analysis of two polar bodies revealed no hybridization with either prob ...
... (Figures 2 and 3); one was PI type M (Figure 3, slot 2B), two were PI type Z (Figure 2, upper polar body, and Figure 3, slot 2C) and two were heterozygous due to crossing over (Figure 2, lower polar body; Figure 3, slot IB). The analysis of two polar bodies revealed no hybridization with either prob ...
Background for the Recombinant DNA Lab
... amplify the region of DNA surrounding the SNP (Fig. 1). We need to use PCR so we will have many copies of the particular DNA we are interested in; we do not want to try to experiment with the entire chromosome (remember how big it was!). We need many copies in order to be able to visualize the DNA o ...
... amplify the region of DNA surrounding the SNP (Fig. 1). We need to use PCR so we will have many copies of the particular DNA we are interested in; we do not want to try to experiment with the entire chromosome (remember how big it was!). We need many copies in order to be able to visualize the DNA o ...
PCR-based Markers and Cut Flower Longevity in Carnation
... with different phenotypes (Arus, 2000). With this aim, we started to analyze ethylene biosynthesis and regulation gene sequences using specific primer sets. Amplification products were obtained in four individuals using 22 primer pairs (Table 2). For three primer pairs (n. 8, 11 and 19) the agarose ...
... with different phenotypes (Arus, 2000). With this aim, we started to analyze ethylene biosynthesis and regulation gene sequences using specific primer sets. Amplification products were obtained in four individuals using 22 primer pairs (Table 2). For three primer pairs (n. 8, 11 and 19) the agarose ...
MOLLECULAR BIOLOGY COURSE
... bands that appear when the chromosome is stained in a certain way. The position is usually designated by two digits (representing a region and a band), which are sometimes followed by a decimal point and one or more additional digits (representing sub-bands within a light or dark area). The number i ...
... bands that appear when the chromosome is stained in a certain way. The position is usually designated by two digits (representing a region and a band), which are sometimes followed by a decimal point and one or more additional digits (representing sub-bands within a light or dark area). The number i ...
The DpnI/DpnII pneumococcal system, defense against foreign
... dsDNA once integrated into the heteroduplex? This could be useful during transformation of homologous DNA with high numbers of methylation sites. In this situation, were the replication fork to pass over the me +/0 heteroduplex before DpnM had rendered every site me +, me0 dsDNA could be produced pr ...
... dsDNA once integrated into the heteroduplex? This could be useful during transformation of homologous DNA with high numbers of methylation sites. In this situation, were the replication fork to pass over the me +/0 heteroduplex before DpnM had rendered every site me +, me0 dsDNA could be produced pr ...
Amino acid
... Major "groove" not really groove Narrow minor groove Base pairs nearly perpendicular to helix axis GpC repeat, not single base-pair – GpC stack: good base overlap – CpG: less overlap. • Zigzag backbone due to C sugar conformation compensating for G glycosidic bond conformation • Conformations: – G; ...
... Major "groove" not really groove Narrow minor groove Base pairs nearly perpendicular to helix axis GpC repeat, not single base-pair – GpC stack: good base overlap – CpG: less overlap. • Zigzag backbone due to C sugar conformation compensating for G glycosidic bond conformation • Conformations: – G; ...
The Close Relationship Between the A and B Genomes in Avena L
... satellited. Thus, this set of chromosomes was designated as B genome (Rajhathy and Morrison, 1959). Although four different genomes (A, B, C, and D) have been designated to Aena species, two of them are present in the diploid level (A and C), three at the tetraploid level (AB and AC), and three at ...
... satellited. Thus, this set of chromosomes was designated as B genome (Rajhathy and Morrison, 1959). Although four different genomes (A, B, C, and D) have been designated to Aena species, two of them are present in the diploid level (A and C), three at the tetraploid level (AB and AC), and three at ...
Specific function of a plastid sigma factor for ndhF
... predominant role in the transcription of photosynthesis-related genes (10). The switch from predominant NEP to predominant PEP activity during plant development might be regulated by glutamyl-tRNA (11). In Arabidopsis, cDNAs encoding six different sigma-like transcription factors that could be invol ...
... predominant role in the transcription of photosynthesis-related genes (10). The switch from predominant NEP to predominant PEP activity during plant development might be regulated by glutamyl-tRNA (11). In Arabidopsis, cDNAs encoding six different sigma-like transcription factors that could be invol ...
Two-Exon Skipping Due to a Point Mutation in p67
... which supported the superoxide generating activity of neutrophil membrane in vitro, that from the patient did not support activity. Normal levels of the large and small subunits of cytochrome bSsRwere found in the patient’s neutrophils by Western blots. Cytosol of the patient’s neutrophils and EBV-t ...
... which supported the superoxide generating activity of neutrophil membrane in vitro, that from the patient did not support activity. Normal levels of the large and small subunits of cytochrome bSsRwere found in the patient’s neutrophils by Western blots. Cytosol of the patient’s neutrophils and EBV-t ...
Agarose Gel Electrophoresis
... dyes and molecular weight markers. Parameters affecting the migration of DNA through agarose gels Agarose concentration. Molecules of linear, duplex DNA (form III) travel through gel matrices at a rate that is inversely proportional to the log of their molecular weight (Helling et al., 1974). The mo ...
... dyes and molecular weight markers. Parameters affecting the migration of DNA through agarose gels Agarose concentration. Molecules of linear, duplex DNA (form III) travel through gel matrices at a rate that is inversely proportional to the log of their molecular weight (Helling et al., 1974). The mo ...
A systematic genome-wide analysis of zebrafish protein
... Since the publication of the human reference genome, the identities of specific genes associated with human diseases are being discovered at an enormous rate. A central problem is that the biological activity of these genes is often unclear. Detailed investigations in vertebrate model organisms, typ ...
... Since the publication of the human reference genome, the identities of specific genes associated with human diseases are being discovered at an enormous rate. A central problem is that the biological activity of these genes is often unclear. Detailed investigations in vertebrate model organisms, typ ...
In the near future, EU thresholds for adventitious
... characteristics of the real-time and seed pool methods, the result for real-time PCR having a very low standard deviation, and therefore a sharp curve, compared to the seed-pool binomial distribution which is more dispersed - despite this a good degree of confidence (P = 8%) could be placed on the r ...
... characteristics of the real-time and seed pool methods, the result for real-time PCR having a very low standard deviation, and therefore a sharp curve, compared to the seed-pool binomial distribution which is more dispersed - despite this a good degree of confidence (P = 8%) could be placed on the r ...
Beads on a string Bowater Biochem Soc Trans 2012
... remodelling around promoter sites was also the subject of the final talk by Tom Owen-Hughes [21], using high-throughput sequencing of yeast strains to align transcription start sites and nucleosome positions. The results indicate that ISW1- and Chd1-containing remodelling enzymes maintain positioned ...
... remodelling around promoter sites was also the subject of the final talk by Tom Owen-Hughes [21], using high-throughput sequencing of yeast strains to align transcription start sites and nucleosome positions. The results indicate that ISW1- and Chd1-containing remodelling enzymes maintain positioned ...
FEBS Letters
... In order to identify and analyze the dxs gene from cyanobacteria, analysis of conserved regions in the dxs gene of E. coli [9] and related sequences of hypothetical proteins in Bacillus subtilis and Synechocystis sp. PCC 6803 was performed. This led us to design suitable oligonucleotides (1for, 1rev ...
... In order to identify and analyze the dxs gene from cyanobacteria, analysis of conserved regions in the dxs gene of E. coli [9] and related sequences of hypothetical proteins in Bacillus subtilis and Synechocystis sp. PCC 6803 was performed. This led us to design suitable oligonucleotides (1for, 1rev ...
Molecular Evolution of the CMT1A-REP Region: A Human
... of the two CMT1A-REP elements (including flanking Alu elements). Predicted sizes of amplification products are based on the sequence for human CMT1A-REP elements (Kiyosawa and Chance 1996). Results for primer set C1/D1, which amplifies the centromeric boundary of the distal REP, are shown in figure ...
... of the two CMT1A-REP elements (including flanking Alu elements). Predicted sizes of amplification products are based on the sequence for human CMT1A-REP elements (Kiyosawa and Chance 1996). Results for primer set C1/D1, which amplifies the centromeric boundary of the distal REP, are shown in figure ...
Precise insertion and guided editing of higher plant
... these reasons, multiple Cpf1 nucleases were tested in our plant transformation system and assays performed to monitor both HDR and NHEJ repair genome edits. To test the capability of each Cpf1 enzyme to generate targeted gene insertions via HDR, a screen was developed that would result in a visual p ...
... these reasons, multiple Cpf1 nucleases were tested in our plant transformation system and assays performed to monitor both HDR and NHEJ repair genome edits. To test the capability of each Cpf1 enzyme to generate targeted gene insertions via HDR, a screen was developed that would result in a visual p ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
... d. a heat-resistant DNA polymerase The correct answer is d— A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is ...
... d. a heat-resistant DNA polymerase The correct answer is d— A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is ...
Antisense derivatives of U7 small nuclear RNA as
... stable small nuclear ribonucleoprotein (snRNP), U7 snRNA expression cassettes, with their small size, will fit into all types of gene therapy vectors so that they can be efficiently targeted to many different tissues and cell types. Toxic side effects have not been observed, and the desired antisens ...
... stable small nuclear ribonucleoprotein (snRNP), U7 snRNA expression cassettes, with their small size, will fit into all types of gene therapy vectors so that they can be efficiently targeted to many different tissues and cell types. Toxic side effects have not been observed, and the desired antisens ...
Milestone2
... The GC content of a genome is the percentage of nucleotides in the genome that are either guanines or cytosines. Different genomes have widely varying GC contents. For example, the genomes of the bacteria Anaeromyxobacter have a GC content of about 75%, whereas the genomes of the bacteria Buchnera h ...
... The GC content of a genome is the percentage of nucleotides in the genome that are either guanines or cytosines. Different genomes have widely varying GC contents. For example, the genomes of the bacteria Anaeromyxobacter have a GC content of about 75%, whereas the genomes of the bacteria Buchnera h ...
PraderWilli syndrome resulting from an unbalanced translocation
... Genomic DNA was extracted from peripheral blood lymphocytes using a Puregene1 DNA isolation kit (Gentra Systems, Minneapolis, MN) according to the manufacturer’s instructions. Methylation analysis for Prader–Willi and Angelman syndromes was performed by methylation polymerase chain reaction (mPCR) a ...
... Genomic DNA was extracted from peripheral blood lymphocytes using a Puregene1 DNA isolation kit (Gentra Systems, Minneapolis, MN) according to the manufacturer’s instructions. Methylation analysis for Prader–Willi and Angelman syndromes was performed by methylation polymerase chain reaction (mPCR) a ...
Applied Microbiology and Biotechnology
... (Stanley et al. 1983). When M. capsulatus is used for SCP production, pMMO formation is preferred because this enzyme allows a more efficient carbon to biomass conversion than sMMO (Jørgensen and Degn 1987). Besides methane, both sMMO and pMMO oxidise several other substrates (Colby and Dalton 1978; ...
... (Stanley et al. 1983). When M. capsulatus is used for SCP production, pMMO formation is preferred because this enzyme allows a more efficient carbon to biomass conversion than sMMO (Jørgensen and Degn 1987). Besides methane, both sMMO and pMMO oxidise several other substrates (Colby and Dalton 1978; ...
Enhanced Detection of Longer Insertions and Deletions in Clinical
... found in the human genome and substantial amount of research, such as that by the International HapMap Consortium, has been focused on accurately mapping and identifying SNVs for human genetic variation studies [8]. However, in spite of being the second most common type of genomic alterations [9], i ...
... found in the human genome and substantial amount of research, such as that by the International HapMap Consortium, has been focused on accurately mapping and identifying SNVs for human genetic variation studies [8]. However, in spite of being the second most common type of genomic alterations [9], i ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).