The DnaE polymerase from Deinococcus radiodurans features
... Deinococcus radiodurans is a Gram-positive bacterium, belonging to the Deinococcales order, whose members feature outstanding radioresistance [30]. D. radiodurans was indeed first isolated from canned meat samples, exposed to γ -rays in order to obtain a sterile, and hence stable, food supply [31]. ...
... Deinococcus radiodurans is a Gram-positive bacterium, belonging to the Deinococcales order, whose members feature outstanding radioresistance [30]. D. radiodurans was indeed first isolated from canned meat samples, exposed to γ -rays in order to obtain a sterile, and hence stable, food supply [31]. ...
Molecular Evolution of Functional Nucleic Acids
... Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine analog with histamine at the 8 position, instead of the corresponding natural nucleotides [24]. Interestingly, the 20 bases o ...
... Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine analog with histamine at the 8 position, instead of the corresponding natural nucleotides [24]. Interestingly, the 20 bases o ...
Nucleotide Sequence Preservation of Human
... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
Ziembinska
... Denaaturing Gradient Gel Electrophoresis DGGE is based on PCR products separation due to its sequence, not DNA fragment size. DNA isolation before the procedure is necessary. It enables fingerprints obtainment, useful in different samples comparison. Cutting DNA band off the gel in order to sequence ...
... Denaaturing Gradient Gel Electrophoresis DGGE is based on PCR products separation due to its sequence, not DNA fragment size. DNA isolation before the procedure is necessary. It enables fingerprints obtainment, useful in different samples comparison. Cutting DNA band off the gel in order to sequence ...
Determination of guanine-plus-cytosine content of
... stained with a combination of DNA-specific fluorochromes, Hoechst 33258 and chromomycin A3, which bind to AT- and GC-rich regions of DNA, respectively. A linear relationship (r = 0.99) was demonstrated between the log of the ratio of chromomycin A3 to Hoechst 33258 fluorescence and the log of the % ...
... stained with a combination of DNA-specific fluorochromes, Hoechst 33258 and chromomycin A3, which bind to AT- and GC-rich regions of DNA, respectively. A linear relationship (r = 0.99) was demonstrated between the log of the ratio of chromomycin A3 to Hoechst 33258 fluorescence and the log of the % ...
Rolling circle transcription on smallest size double stranded DNA
... DNA nanotechnology is the application of DNA as a material to construct nano scale patterns or structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural guides for other mo ...
... DNA nanotechnology is the application of DNA as a material to construct nano scale patterns or structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural guides for other mo ...
Analysis of Flanking Sequences from Dissociation
... to 45% of all useful insertions sequenced (356/790; see Table 1), which is comparable to the fraction of the sequence of the Arabidopsis genome available from public databases at the time of analysis. Figure 2 shows the distribution of these insertions. For purposes of illustration, we used a public ...
... to 45% of all useful insertions sequenced (356/790; see Table 1), which is comparable to the fraction of the sequence of the Arabidopsis genome available from public databases at the time of analysis. Figure 2 shows the distribution of these insertions. For purposes of illustration, we used a public ...
Analysis of Flanking Sequences from Dissociation
... to 45% of all useful insertions sequenced (356/790; see Table 1), which is comparable to the fraction of the sequence of the Arabidopsis genome available from public databases at the time of analysis. Figure 2 shows the distribution of these insertions. For purposes of illustration, we used a public ...
... to 45% of all useful insertions sequenced (356/790; see Table 1), which is comparable to the fraction of the sequence of the Arabidopsis genome available from public databases at the time of analysis. Figure 2 shows the distribution of these insertions. For purposes of illustration, we used a public ...
Fusobacterium pseudonecrophorurn Is a Synonym for Fusobacten
... (12) fail to discriminate among Fusobacterium species. For example, cat strains of F. alocis cannot be distinguished from F. simiae or F. necrophorum on the basis of these phenotypic tests (11). Furthermore, many of the phenotypic tests considered discriminatory of species (12) depend for their accu ...
... (12) fail to discriminate among Fusobacterium species. For example, cat strains of F. alocis cannot be distinguished from F. simiae or F. necrophorum on the basis of these phenotypic tests (11). Furthermore, many of the phenotypic tests considered discriminatory of species (12) depend for their accu ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
... each other (Desjardins and Morais, 1990; 199 1). In contrast to the vertebrates, invertebrates have undergone significant rearrangements in mtDNA gene order. Multiple inversions and translocations involving numerous loci are evident when the mtDNA genomes are compared between insects, sea urchins an ...
... each other (Desjardins and Morais, 1990; 199 1). In contrast to the vertebrates, invertebrates have undergone significant rearrangements in mtDNA gene order. Multiple inversions and translocations involving numerous loci are evident when the mtDNA genomes are compared between insects, sea urchins an ...
Biochemistry
... (The capacity of the various polymerases to synthesize different RNAs was shown with the toxin-amanitin. At low concentrations of toxin-amanitin synthesis of mRNAs are affected but not rRNAs nor tRNAs. At high concentrations, both mRNAs and tRNAs are affected. These observations have allowed the ide ...
... (The capacity of the various polymerases to synthesize different RNAs was shown with the toxin-amanitin. At low concentrations of toxin-amanitin synthesis of mRNAs are affected but not rRNAs nor tRNAs. At high concentrations, both mRNAs and tRNAs are affected. These observations have allowed the ide ...
D-loop - BioMed Central
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
Powerpoint show
... 3. Determination of region in which polymorphic markers are tightly linked – no recombinants 4. Contig assembly and sequence analysis of region 5. Compare polymorphisms in candidate gene between normal and disease chromosomes to establish all affected family members have mutation 6. Test expression ...
... 3. Determination of region in which polymorphic markers are tightly linked – no recombinants 4. Contig assembly and sequence analysis of region 5. Compare polymorphisms in candidate gene between normal and disease chromosomes to establish all affected family members have mutation 6. Test expression ...
The Wiskott-Aldrich Syndrome and X-Linked
... severity of WAS-associated symptoms was scored from 1 to 5 , based on the following criteria. A score of 1 was given to patients with thrombocytopenia and small sized platelets, but without any other symptoms or clinical findings. Patients with platelet abnormalities and a history of mild, transient ...
... severity of WAS-associated symptoms was scored from 1 to 5 , based on the following criteria. A score of 1 was given to patients with thrombocytopenia and small sized platelets, but without any other symptoms or clinical findings. Patients with platelet abnormalities and a history of mild, transient ...
genetic mapping and dna sequencing of the loblolly pine genome
... from the four maps is shown in Fig. 1. There are markers that mapped on both the maternal and paternal linkage group of one but not both pedigrees. These are designated as fully informative markers and are used to join maternal and paternal linkage groups; examples included 2536a (QTL map) and 2564c ...
... from the four maps is shown in Fig. 1. There are markers that mapped on both the maternal and paternal linkage group of one but not both pedigrees. These are designated as fully informative markers and are used to join maternal and paternal linkage groups; examples included 2536a (QTL map) and 2564c ...
24.5 Nucleic Acids
... The translation of a base sequence of DNA in a gene into the amino acid sequence of a peptide begins with the initiation code word and runs continuously until a termination code word is reached. • The termination code word signals a stop to the addition of amino acids in the production of the peptid ...
... The translation of a base sequence of DNA in a gene into the amino acid sequence of a peptide begins with the initiation code word and runs continuously until a termination code word is reached. • The termination code word signals a stop to the addition of amino acids in the production of the peptid ...
Comparative Sequence Analysis of a Region on Human
... Structure, Molecular Cloning, and FISH Analysis of Mouse Genomic DNA To obtain mouse genomic DNA homologous to the region of interest on chromosome 13q14 (Corcoran et al., 1998), a mouse genomic PAC library was screened with a series of probes as described under Materials and Methods. A total of 22 ...
... Structure, Molecular Cloning, and FISH Analysis of Mouse Genomic DNA To obtain mouse genomic DNA homologous to the region of interest on chromosome 13q14 (Corcoran et al., 1998), a mouse genomic PAC library was screened with a series of probes as described under Materials and Methods. A total of 22 ...
Comparison of the Detection Limits of the Culture and PCR Methods
... Objectives: The aim of this study was to compare the detection limits of the PCR and culture methods for the diagnosis of Campylobacter spp., Yersinia spp., Clostridium perfringens, and Clostridium difficile in human stool samples. Methods: Healthy human stool and sterile phosphate-buffered saline ( ...
... Objectives: The aim of this study was to compare the detection limits of the PCR and culture methods for the diagnosis of Campylobacter spp., Yersinia spp., Clostridium perfringens, and Clostridium difficile in human stool samples. Methods: Healthy human stool and sterile phosphate-buffered saline ( ...
A novel gene encoding a 54 kDa polypeptide is
... rapidly and unambiguously from environmental samples. Since methane could also be of recent geological origin, methane-utilizing bacteria were not considered in our investigation. The conventional method for detection of specific microorganisms is selective plating. Although easy to use, this method ...
... rapidly and unambiguously from environmental samples. Since methane could also be of recent geological origin, methane-utilizing bacteria were not considered in our investigation. The conventional method for detection of specific microorganisms is selective plating. Although easy to use, this method ...
Analysis of aptamer sequence activity relationshipsw
... aptamer design in a manner analogous to the Quantitative Structure–Activity Relationship (QSAR) assessments used during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinit ...
... aptamer design in a manner analogous to the Quantitative Structure–Activity Relationship (QSAR) assessments used during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinit ...
Stochastic processes and Markov chains (part II)
... factors and motifs are known. Hence,, a Not all transcription high occurrence of a particular sequence pattern in the upstream regions of a gene may indicate that it has a regulatory function f (e.g., ...
... factors and motifs are known. Hence,, a Not all transcription high occurrence of a particular sequence pattern in the upstream regions of a gene may indicate that it has a regulatory function f (e.g., ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... gel could be stored in a plastic bag and refrigerated after the experiment, there may be limits. Depending on buffer during electrophoresis in order to generate a suitable electric current and to reduce the heat generated by electric current can be considered as limitations of electrophoretic techni ...
... gel could be stored in a plastic bag and refrigerated after the experiment, there may be limits. Depending on buffer during electrophoresis in order to generate a suitable electric current and to reduce the heat generated by electric current can be considered as limitations of electrophoretic techni ...
High-resolution mapping of the leaf rust disease resistance gene Lr1
... The leaf rust disease resistance gene 1 (Lr1) described by Ausemus et al. (1946) was identified in the hexaploid wheat cultivar Malakoff (Dyck and Samborski 1968) and is present in a number of wheat cultivars (Knott 1989; McIntosh 1988). Plants containing the Lr1 resistance gene show a typical hyper ...
... The leaf rust disease resistance gene 1 (Lr1) described by Ausemus et al. (1946) was identified in the hexaploid wheat cultivar Malakoff (Dyck and Samborski 1968) and is present in a number of wheat cultivars (Knott 1989; McIntosh 1988). Plants containing the Lr1 resistance gene show a typical hyper ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).